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All Journal Jurnal Farmasi Indonesia Jurnal Kefarmasian Indonesia Jurnal Insan Farmasi Indonesia
Lukito Mindi Cahyo
Program Studi Farmasi, Fakultas Farmasi, Universitas Setia Budi, Surakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Physics

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Journal : Jurnal Insan Farmasi Indonesia

 1 
ISOLASI DAN IDENTIFIKASI BAKTERI PENGHASIL PROTEASE EKSTRASELULER DARI LIMBAH CAIR INDUSTRI TAHU Wiwi Kusumasari; Ana Indrayati; Lukito Mindi Cahyo
Jurnal Insan Farmasi Indonesia Vol 6 No 1 (2023): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v6i1.1266

Abstract

Tofu waste that is discharged into the aquatic environment has a negative impact on the quality of the water around the tofu liquid waste disposal site. Industrial enzyme production is currently dominated by proteases. Proteases in the pharmaceutical field support the absorption of proteins in the digestive tract, as active cosmetic ingredients. This study aims to isolate, identify protease-producing bacteria from tofu wastewater, determine the total protein content and enzyme activity index of 5 isolates of protease-producing bacteria. Tofu wastewater bacteria were isolated with Skim Milk Agar medium. 5 bacterial isolates were selected which produced the widest clear zones, different colony characters, identified by morphological tests, gram staining tests, and biochemical tests. Five isolates were made into a bacterial suspension isolated from crude protease extract and the protease level was determined using the Bicinchoninic Acid method, and the protease activity was tested. Five bacterial colonies showed that colony 1 and colony 2 included Gram positive bacteria, colonies 3, 4 and 5 included Gram negative bacteria. The value of total protein content in the tofu industrial wastewater from the five colonies respectively: 219.93; 833,267; 421,267; 81,267; and 38.6 µg/µL. The highest proteolytic activity test was in sample 2 of 2.13.
Co-Authors Ana Indrayati Avianti Dewi Purwaningsih Deva Yolanda Dwi Nur Azizah Ghani Nurfiana Fadma Sari Masyitah Novia Tri Wijayanti Wiwi Kusumasari