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Ethnobotany Medicinal Plants of Dawan Ethnic in North Central Timor Regency Noviana Mery Obenu; Emilia Juliyanti Bria
Biotropika: Journal of Tropical Biology Vol. 9 No. 3 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2021.009.03.09

Abstract

The use of medicinal plants is still maintained for generations by the Dawan Tribe in North Central Timor Regency. The loss of knowledge about the use of plants as an alternative treatment of various diseases used by local communities can be caused by the impact of modernization. Therefore, it is necessary to carry out the process of documenting this knowledge. The purpose of this research is to take an inventory of medicinal plants, to know the plant organs used for medicine and how to use the medicine. The method used in this study is a qualitative description method, with several field data collection techniques and identification of medicinal plant species. Data collection techniques include observation, interviews, and exploration. The results of this study indicate that there are 44 species of medicinal plants used, from 25 plant families. The medicinal plant family with a high number of species is the Zingiberaceae family (13.6%). Based on the habitus, 44 species of medicinal plants used consisted of 23 species from trees, 2 species from lianas, 9 species from herbs, 7 species from shrubs, and 3 species from shrubs. In addition, based on plant organs the most widely used as medicine out of 44 plant species, namely the part of the leaf organ (23.91%). Meanwhile, based on the method of use of the 44 species of plants used for medicinal ingredients, the method of drinking has the highest percentage (33.33%) and is usually practiced by the Dawan Tribe.
Ekstraksi dan Identifikasi Komposisi Metabolit Fraksi Diklorometana dan Aquades Ektrak Metanol Daun Sirsak (Annona muricata Linn) Noviana Mery Obenu
Saintek Lahan Kering Vol 2 No 1 (2019): JSLK Juni 2019
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.971 KB) | DOI: 10.32938/slk.v2i1.717

Abstract

Soursop is one of the many plants found in East Nusa Tenggara (NTT). Some parts of the soursop plant are known to have been widely used as traditional medicine or as natural pesticides. The use of plants as a treatment or as a natural pesticide cannot be separated from the bioactive compounds contained therein. These compounds will be successfully isolated depending on the choice of solvent. This research was conducted with the aim of knowing the composition of the metabolites contained in each solvent used. The steps of this study include preparation of soursop leaf samples, extraction by maceration with methanol solvents, partitioning of methanol extract with dichloromethane and water solvents and identification of metabolite compositions with high-performance liquid chromatography (HPLC) instruments. The compounds identified in HPLC dichloromethane solvents are thought to be phenol, polyketide, tannin and alkaloid metabolites. Whereas HPLC identified compounds in water solvents are thought to be polar compounds which are bound to a glycoside group
Identification Chemical Compositions of Lemongrass Plant (Cymbopogon nardus L.) Dawan Tribe, Oenenu Village, North Central Timor Regency Noviana Obenu; Eduardus Edi; Risna E. Adu
Jurnal Akademika Kimia Vol. 10 No. 2 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.673 KB) | DOI: 10.22487/j24775185.2021.v10.i2.pp93-97

Abstract

Lemongrass (Cymbopogon nardus L.) is a local natural resource used by the Dawan tribe as an alternative for mosquito repellent. The utilization of these plants cannot be separated from the content of compounds found in these plants. Therefore, it is necessary to analyze the content of bioactive compounds from lemongrass (Cymbopogon nardus L.). This research method includes sample preparation, isolation and identification of compounds, and antioxidant activity analysis. The results of this study that there were three main components of citronella oil, namely geraniol, citronellol, and citronellal, with a higher percentage of geraniol, namely 18.82%. The compounds contained in lemongrass oil have a weak antioxidant activity with IC50 681.48 mg/mL.
Pengaruh Perlakuan Awal Ampas Biji Jewawut (Setaria italica L.) dengan Microwave Irradiation Untuk Produksi Bioetanol Sefrinus Maria Dolfi Kolo; Noviana Mery Obenu; Natalia Tige Rohy
ALCHEMY Jurnal Penelitian Kimia Vol 18, No 2 (2022): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.18.2.59819.183-192

Abstract

Produksi energi terbarukan termasuk bioetanol menjadi alternatif penganti bahan bakar fosil. Salah satunya dari ampas jewawut karena memiliki kandungan selulosa sebesar 32,41% sehingga sangat potensial dan ekonomis sebagai sumber energi baru terbarukan. Penelitian ini bertujuan mengetahui morfologi permukaan, suhu dan konsentrasi H2SO4 optimum pada proses hidrolisis menggunakan microwave dan kadar bioetanol dari hidrolisat ampas biji jewawut. Penelitian ini terdiri atas empat tahapan yaitu proses hidrolisis menggunakan microwave, fermentasi, distilasi dan pengujian kadar bioetanol baik secara kualitatif maupun kuantitatif menggunakan metode berat jenis dan kromatografi gas. Hidrolisis dilakukan melalui variasi suhu 75℃, 100℃, 125℃, 150℃, dan 175℃ dan konsentrasi H2SO4 0,5%, 1%, 2%, 5% dan 7%. Hasil penelitian menunjukkan bahwa morfologi permukaan sampel sebelum hidrolisis memiliki permukaan yang datar, kasar dan kaku namun setelah dihidrolisis permukaan sampel menjadi rapuh dan halus. Analisa gula pereduksi menggunakan pereaksi DNS (Dinitrosalisilat) diperoleh suhu optimum microwave yaitu pada suhu 150℃ dengan kadar gula pereduksi sebesar 25,3 g/L dan konsentrasi H2SO4 optimum pada 5% dengan kadar gula pereduksi sebesar 32,8 g/L. Uji kualitatif dari hasil fermentasi dan distilasi menunjukkan pada sampel mengandung bioetanol yang ditandai dengan terjadinya perubahan warna kalium dikromat dari warna jingga menjadi hijau kebiruan. Kadar bioetanol yang diperoleh dengan metode berat jenis sebesar 5% dan 6,08% dari analisa dengan kromatografi gas.Pretreatment Effect of Barley Seed Dregs (Setaria italica L.) with Microwave Irradiation for Bioethanol Production. Renewable energy production, including bioethanol, is an alternative to fossil fuels. One of the alternative sources is barley dregs because it has a cellulose content of 32.41%; thus, it is very potential and economical as a new renewable energy source. This study aims to determine the surface morphology, temperature, and optimum H2SO4 concentration in the hydrolysis process using a microwave and the bioethanol content of the hydrolyzed barley seed dregs. The research comprised four steps: hydrolysis, fermentation, distillation, and qualitatively and quantitatively analysis of bioethanol levels using specific gravity and gas chromatography methods. Hydrolysis was carried out by varying the temperature of 75℃, 100℃, 125℃, 150℃, and 175℃, and the concentration of H2SO4 was 0.5%, 1%, 2%, 5%, and 7%. The results showed that the surface morphology of the sample before hydrolysis had a flat, rough and rigid surface; however, after hydrolysis, the sample's surface became brittle and smooth. Analysis of reducing sugar using DNS reagent (dinitrosalicylate) obtained the optimum microwave temperature at 150℃ with a reducing sugar content of 25.3 g/L and an optimum concentration of H2SO4 at 5% with a reducing sugar content of 32.8 g/L. The qualitative test of the fermentation and distillation results shows the samples containing bioethanol marked by the changes in potassium dichromate colors from orange to bluish-green. The bioethanol content obtained by the specific gravity method was 5% and 6.08% from analysis by gas chromatography.
Permainan Sains Menggunakan Bahan Sederhana bagi Siswa/i SMP Negeri II Koko Kecamatan Kota Kefamenanu, Kabupaten TTU Noviana Obenu; Janrigo Klaumegio Mere; Gebhardus D. Gelyaman; Emilia Juliyanti Bria; Yoseph Satrio Akoit; Patrisius Maryanto Bria
Jurnal Abdi Masyarakat Indonesia Vol 2 No 6 (2022): JAMSI - November 2022
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.506

Abstract

Pendidikan yang baik adalah pendidikan yang dapat meningkatkan daya literasi, daya belajar, inovasi dan kreatifitas siswa. Salah satu cara dalam penyampaian proses belajar mengajar yang lebih optimal adalah dengan penyiapan media belajar yang menarik. Media belajar dapat dilakukan dengan pertunjukkan permaianan sains. Kegiatan pengabdian ini dilaksanakan di SMP Negeri II Koko dengan dihadiri siswa/I kelas VII, VIII, dan IX. Materi pengabdian berupa permainan sains berupa Pesan rahasia, Magnet listrik, Peristiwa melayang tenggelam dan terapung, Gunung meletus dan Listrik buah. Kegiatan permainan atau demonstrasi sains ini dilakukan untuk memperkenalkan kepada siswa-siswi pembelajaran sains yang lebih menarik. Kegiatan ini dilaksanakan dengan metode pertunjukkan, ceramah dan diskusi. Dapat disimpulkan bahwa siswa – siswi berinteraksi secara aktif, tidak kaku, penuh semangat ditandai dengan banyaknya pertanyaan yang muncul, dan siswa sangat antusias dalam menjawab pertanyaan yang diberikan oleh tim pengabdi serta tingginya minat siswa untuk terlibat di dalam praktik demonstrasi sains.
PELATIHAN PEMBUATAN HERBARIUM KERING SEBAGAI MEDIA PEMBELAJARAN DI SEKOLAH MENENGAH PERTAMA NEGERI 2 KEFAMENANU Emilia Juliyanti Bria; Noviana Mery Obenu; Janrigo Kalumegio Mere
Jurnal Pasopati Vol 5, No 1 (2023)
Publisher : Fakultas Teknik Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/pasopati.2023.17369

Abstract

Media pembelajaran merupakan salah satu komponen penting dalam proses belajar-mengajar. Salah satu media pembelajaran yang mendukung pembelajaran berbasis lingkungan di tingkat sekolah menengah pertama khususnya metapelajaran IPA adalah herbarium. Tujuan pengabdian ini adalah memberikan pengetahuan tentang herbarium dan keterampilan bagaimana teknik membuat herbarium secara sederhana bagi siswa di SMP Negeri 2 Kefamenanu. Metode yang digunakan adalah tutorial dan pendampingan secara kelompok kepada siswa. Hasil kegiatan ini menunjukkan bahwa siswa-siswi sangat antusias dalam mengikuti pelatihan yang ditunjukkan dengan diperolehnya pengetahuan dan keterampialn baru tentang herbarium dan teknik membuatnya secara sederhana. Selain itu, diperoleh juga sebelas spesies tumbuhan yang teridentifikasi nama ilmiahnya yang dibuat herbarium dari lingkungan sekolah dan dapat dijadikan sebagai perangkat pembelajaran dalam menunjang tercapainya tujuan pembelajaran.Kata kunci : pelatihan, herbarium, media pembelajaran, Kefamenanu
Optimasi Proses Hidrolisis Rumput Laut Ulva Reticulata dengan Pelarut HNO3 untuk Produksi Bioetanol Sefrinus Maria Dolfi Kolo; Noviana Mery Obenu; Lusitania Kefi; Felicitas F.Fuel
Jurnal Riset Kimia Vol. 14 No. 1 (2023): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v14i1.574

Abstract

One alternative to make biofuels and replace petroleum-based fuels is to convert non-food ingredients from Ulva reticulata seaweed into bioethanol. Seventy percent of the earth's surface is covered by microalgae and seaweeds that can be converted into bioethanol. Ulva seaweed contains 50.3% carbohydrates in the form of heteropolysaccharides  such as glucose, arabinose, rhamnose and xylose. Optimization of the seaweed hydrolysis catalyzed by HNO3 using Microwave irradiation was done by varying acid concentration (1, 3, 5, 7%), hydrolysis time (30, 40, 50, and 60 minutes), and hydrolysis temperature (75, 100, 125, 150°C). Fermentation was carried out by varying inoculum concentrations (6, 8, and 10% (v/v)) for 5, 6 and 7 days at a temperature of 30°C and a pH of 4.5. Analysis of the surface texture of the sample was carried out by Scanning Electron Microscopy (SEM). The analysis of reducing sugars concentration was carried out using the dinitrosalicylate (DNS) method. Ethanol analysis was carried out by Gas Chromatography (GC). The results of SEM analysis showed that prior to hydrolysis, the surface morphology of the powder was still compact and intact. Whereas after being hydrolyzed with HNO3 it was seen that the surface texture of the powder suffered significant damage. The hydrolysis results showed that the optimum conditions during the pretreatment of U. reticulata powder was at acid concentration of 7%, reaction time of 50 minutes, reaction temperature of 150°C and 250 watts of power which gave hydrolysate with reducing sugar concentration of 86.5 g/L. Fermentation of the hydrolysate using yeast Saccharomyces cerevisiae produced bioethanol with concentration of 37.2% as analyzed using a gas chromatograph.
Analisis Fitokimia Ekstrak Pelarut Kulit Akar Tumbuhan “At Anonse” (Annona reticulata L.) Maria Kasilda Elu; Oktovianus Kasa; Maria Aprista Manikin; Noviana Mery Obenu; Eduardus Edi
Saintek Lahan Kering Vol 6 No 1 (2023): JSLK JUNI 2023
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/slk.v6i1.2188

Abstract

Medicinal plants are plants that can be used as medicine or medicinal ingredients to treat various diseases. One of the plants used as a medicinal plant is Annona reticulata L. This study aims to determine the content of secondary metabolites in the solvent extract of the root bark of the plant “At Anonse” (Annona reticulata L.). The stages of this research were sample preparation, extraction, and phytochemical analysis. Extraction was carried out by maceration using n-hexane, ethyl acetate, and methanol as solvents. Phytochemical analysis was carried out qualitatively based on changes in color, precipitate and foam. The results of the phytochemical analysis test showed that the groups of compounds contained in the root bark of the plant “At Anonse” were triterpenoids, tannins, flavonoids, phenolics, alkaloids and saponins.
ANALISIS FITOKIMIA EKSTRAK POLAR KULIT AKAR TUMBUHAN “At Anonse” (Annona reticulata L.) Maria Kasilda Elu; Noviana Mery Obenu; Marselina Theresia Djue Tea
Journal of Chemical Science and Application Vol 1 No 2 (2023): Natural Resources
Publisher : Program Studi Kimia Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jcsa.v1i2.4229

Abstract

Penelitian mengenai analisis fitokimia ekstrak polar kulit akar tumbuhan “At Anonse” (Anonna reticulata L.) telah dilakukan. Penelitian ini bertujuan untuk mengetahui kandungan senyawa metabolit sekunder dan uji aktivitas antioksidan pada ekstrak polar kulit akar tumbuhan “At Anonse” (Annona reticulata L.). Tahapan penelitian ini yakni preparasi sampel, ekstraksi, skrining fitokimia dan uji aktivitas antioksidan. Ekstraksi dilakukan secara maserasi menggunakan pelarut metanol dan uji aktivitas antioksidan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Hasil penelitian diperoleh uji skrining fitokimia menunjukkan golongan senyawa yang terkandung dalam kulit akar tumbuhan “At Anonse” adalah golongan triterpenoid, flavonoid, fenolik, alkaloid dan saponin.
ANALISIS FITOKIMIA EKSTRAK POLAR DAUN TUMBUHAN “At Anonse” (Annona reticulata L.) Kresensia Siki; Noviana Mery Obenu; Eduardus Edi
Journal of Chemical Science and Application Vol 1 No 2 (2023): Natural Resources
Publisher : Program Studi Kimia Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jcsa.v1i2.4233

Abstract

Tumbuhan obat adalah tumbuhan yang telah diidentifikasi berdasarkan pengamatan manusia yang memiliki senyawa yang bermanfaat untuk mencegah dan menyembuhkan penyakit. Penelitian ini bertujuan untuk mengetahui kandungan senyawa pada ekstrak polar daun tumbuhan “At Anonse” (Annona reticulata L.). Tahapan penelitian ini meliputi pengambilan sampel, preparasi sampel, skrining fitokimia. Ekstraksi dilakukan secara maserasi menggunakan pelarut metanol. Hasil penelitian diperoleh uji skrining fitokimia menunjukkan golongan senyawa yang terkandung dalam daun tumbuhan “At Anonse” adalah golongan flavonoid, saponin, alkaloid, tanin, fenolik, triterpenoid dan steroid.