Garuda - Garba Rujukan Digital

Comparison of Formulation Methods to Produce Nano-Chitosan as Inhibitor Agent for Bacterial Growth Nugraheni, Prihati Sih; Soeriyadi, Alexander H.; Ustadi, Ustadi; Sediawan, Wahyudi Budi; Budhijanto, Wiratni
Journal of Engineering and Technological Sciences Vol 51, No 3 (2019)
Publisher : ITB Journal Publisher, LPPM ITB

Chitosan is known as an antibacterial agent. The effective surface area ratio of chitosan can be increased by modification into nanoparticles. Nano-chitosan can be prepared with several simple methods, i.e. precipitation, ionic gelation, or the polyelectrolyte complex method. This study compared these three methods in terms of the targeted product characteristics, i.e. stability of the average nanoparticle size as well as the colloidal dispersion, and the antibacterial characteristics. All three methods resulted in nanoparticle formation, but in the precipitation method significant zeta potential reduction was observed due to the presence of negative ions from the alkali that neutralized the chitosan amine group. The ionic gelation method yielded higher zeta potential and higher inhibition of bacterial growth than those yielded by the polyelectrolyte complex method. Ionic gelation and the polyelectrolyte complex method resulted in much better colloidal dispersion stability than the precipitation method, where a significant particle size increase was observed after one week of storage. This result indicates that both ionic gelation and the polyelectrolyte complex method can be used for forming nano-chitosan for the purpose of food preservation. However, for fishery products it is advisable to use the polyelectrolyte complex method because the TPP usually used in ionic gelation is not allowed to be applied to fish.

Antioxidant Activity and Consumer Preference of Yoghurt Enriched with Sargassum polycystum Extract Amir Husni; Maria Madalena; U. Ustadi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 18 No 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Research on the addition of antioxidants to yogurt with ethanolic extract ofSargassum polycystum has been done. This study aims were to determine the effect ofethanolic extract of S. polycystum into the yoghurt to the antioxidant activity and thelevel of consumer acceptance as well as determine the effect of ethanolic extract ofS. polycystum of chemical composition on yoghurt. S. polycystum was extracted usingethanol 96%. The ethanolic extract were inforfified into yoghurt drink and tested theantioxidant activity and the chemical compositionas well as consumer acceptance test.Fermented yoghurt fortified with ethanolic extract of S. polycystum of 0.00; 0.22; 0.44;0.66, 0.88, and 1.10%. The results showed that the addition of ethanolic extract was notsignificant (p>0.05) on pH, but significant (p<0.05) to total lactic acid, antioxidant activity,chemical composition (proximate), and consumer acceptance.Keywords: Antioxidant activity, ethanol cextract, Sargassum polycystum, consumeracceptance, yoghurt

Development of Alginate Extraction Method from Seaweed Turbinaria ornata Rani Laksanawati; U. Ustadi; Amir Husni
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Indonesia has a lot of high potential seaweed as a source of an alginate, but the extraction method had been used was not suitable. The aim of this research is to develop alginate extraction method from Turbinaria ornata seaweed with calcium method and compare the cost of extraction with an acid method as a control. In this research, various concentration of calcium chloride (CaCl2) was used in the separation of alginate from the extracted filtrate. The concentration of CaCl2 used varies from 0.50; 0.75; 1.00; 1.25; and 1.50 M. For comparison, alginate extraction with acid (control) method was used. Alginate quality parameters observed included alginate yield, product color degree, and gel viscosity. The results showed that the yield of alginate produced by calcium method was 36.89; 44.00; 56.00; 52.89 and 53.11% and for control 22.45%. From the degree of product color, CaCl2 concentration did not significantly affect the color of alginate but was darker when compared to the product extracted by the acid method. The viscosity of alginate produced by calcium method was 27,69; 26,57; 24.50; 22.41 and 19.92 cP while for control 32.88 cP. The extraction of Na-alginate with calcium method can decrease the need for Na-alginate extraction cost by 85% from the amount of Na-alginate extraction cost requirement by the acid method.

Comparison of Formulation Methods to Produce Nano-Chitosan as Inhibitor Agent for Bacterial Growth Prihati Sih Nugraheni; Alexander H. Soeriyadi; Ustadi Ustadi; Wahyudi Budi Sediawan; Wiratni Budhijanto
Journal of Engineering and Technological Sciences Vol. 51 No. 3 (2019)
Publisher : Institute for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.eng.technol.sci.2019.51.3.9

Chitosan is known as an antibacterial agent. The effective surface area ratio of chitosan can be increased by modification into nanoparticles. Nano-chitosan can be prepared with several simple methods, i.e. precipitation, ionic gelation, or the polyelectrolyte complex method. This study compared these three methods in terms of the targeted product characteristics, i.e. stability of the average nanoparticle size as well as the colloidal dispersion, and the antibacterial characteristics. All three methods resulted in nanoparticle formation, but in the precipitation method significant zeta potential reduction was observed due to the presence of negative ions from the alkali that neutralized the chitosan amine group. The ionic gelation method yielded higher zeta potential and higher inhibition of bacterial growth than those yielded by the polyelectrolyte complex method. Ionic gelation and the polyelectrolyte complex method resulted in much better colloidal dispersion stability than the precipitation method, where a significant particle size increase was observed after one week of storage. This result indicates that both ionic gelation and the polyelectrolyte complex method can be used for forming nano-chitosan for the purpose of food preservation. However, for fishery products it is advisable to use the polyelectrolyte complex method because the TPP usually used in ionic gelation is not allowed to be applied to fish.

Penggunaan Ekstrak Rumput Laut Padina sp. untuk Peningkatan Daya Simpan Filet Nila Merah yang Disimpan pada Suhu Dingin Amir Husni; Ustadi Ustadi; Andi Hakim
agriTECH Vol 34, No 3 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The aim of the research was to determine effect of Padina sp. extract on shelf life of red nile ﬁ llet during storage at low temperature. Red nile ﬁ llet was soaked up into Padina sp. extract solution at various concentration of 0; 0.5%; 1%; 1.5%; and 2% for 30 minutes, then was stored in chilling room for 10 days. The observation was carried out every 2 days. The parameters observed were pH, Total Plate Count (TPC), Total Volatile Base-Nitrogen (TVB-N), and organoleptic still be tests. The results showed that the different concentration of Padina sp. extract yielded the signiﬁ cant effect (P<0,05) on pH, TPC, TVB-N and organoleptic of the red nile ﬁ llet. The treatment of Padina sp. extract on red nile ﬁ llet can still be consumed up to 8 days of storage based on TPC, TVB, and organoleptic namely odor and texture. The treatment 1% of Padina sp. extract was the best treatment in maintaining shelf life of red nile ﬁ llet stored at low temperature.ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak Padina sp. terhadap daya simpan ﬁ let nila merah selama penyimpanan pada suhu dingin. Filet nila merah direndam selama 30 menit dalam larutan ekstrak Padina sp. dengan konsentrasi: kontrol; 0,5%; 1%; 1,5%; dan 2%, kemudian disimpan pada chilling room selama 10 hari dengan selang waktu pengamatan setiap 2 hari. Parameter yang diamati meliputi: pH, Total Plate Count (TPC), Total Volatile Base-Nitrogen (TVB-N), dan organoleptik. Hasil penelitian menunjukkan bahwa penggunaan ekstrak Padina sp. yang berbeda memberikan pengaruh nyata (P<0,05) terhadap nilai pH, kandungan bakteri total, kandungan TVB-N, dan nilai organoleptik keseluruhan ﬁ let nila merah selama 10 hari penyimpanan pada suhu dingin. Filet nila merah yang diberi ekstrak Padina sp. masih layak dikonsumsi sampai hari ke-8. Penggunaan ekstrak Padina sp. 1% merupakan perlakuan terbaik dalam mempertahankan kesegaran ﬁ let nila merah yang disimpan pada suhu dingin.

Pengembangan Metode Ekstraksi Alginat dari Rumput Laut Sargassum sp. sebagai Bahan Pengental Amir Husni; Subaryono Subaryono; Yudi Pranoto; Taswir Taswir; Ustadi Ustadi
agriTECH Vol 32, No 1 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Indonesia has a lot of seaweed that have high potential as a producer of alginate, but the method of extraction has not been as expected. The objective of this study to develop a method of extraction of sodium alginate from seaweed Sargassum through the calcium alginate pathway. This study used different variations of the concentration of calcium chloride. The concentration of calcium chloride used varied 0.5, 0.75 and 1 M. As a control, the extraction of alginate performed through alginic acid pathway which was developed at the Center for Research Product Processing and Biotechnology of Marine and Fisheries, Jakarta. Quality parameters were observed including alginate yield, product appearance, viscosity and gel strength. The result showed that the yield of alginate produced successively for 32.67; 44.67 and 53.33 % and 31.67 % for controls. In appearance, the concentration of calcium chloride did not significantly affect the alginate product appearance, but darker when compared with the product extracted through the alginic acid. Viscosity alginate produced successively 149, 131 and 144 cP, while 304 cP for control. In general, the alginate gel strength extracted through of calcium alginate pathway is lower than the alginic acid pathway.ABSTRAKIndonesia mempunyai banyak rumput laut yang berpotensi tinggi sebagai penghasil alginate, namun metode ekstraksinya belum sesuai yang diharapkan. Penelitian ini bertujuan untuk mengembangkan metode ekstraksi natrium alginat dari rumput laut Sargassum melalui jalur kalsium alginat. Dalam penelitian ini digunakan berbagai variasi konsentrasi kalsium klorida yang digunakan pada pemisahan alginat dari filtrat hasil ekstraksinya. Konsentrasi kalsium klorida yang digunakan divariasi 0,5; 0,75 dan 1 M. Sebagai kontrol dilakukan ekstraksi alginat melalui jalur asam alginat yang dikembangkan di Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jakarta. Parameter kualitas alginat yang diamati meliputi rendemen alginat, kenampakan produk, viskositas dan kekuatan gel yang dihasilkan. Hasil pengamatan menunjukkan bahwa rendemen alginat yang dihasilkan berturut-turut sebesar 32,67; 44,67 dan 53,33 % dan untuk kontrol 31,67 %. Secara kenampakan, konsentrasi kalsium klorida tidak terlalu mempengaruhi kenampakan produk alginat yang dihasilkan, tetapi lebih gelap jika dibandingkan dengan produk hasil ekstraksi melalui jalur asam alginat. Viskositas alginat yang dihasilkan berturut-turut 149, 131 dan 144 cP, sementara untuk kontrol 304 cP. Secara umum kekuatan gel alginat yang dihasilkan dari jalur kalsium alginat lebih rendah dibandingkan dengan yang diekstrak melalui jalur asam alginat.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search