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All Journal AQUATIC SCIENCE & MANAGEMENT JURNAL PESISIR DAN LAUT TROPIS
Paulus, James J.H.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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AKTIVITAS ANTIBAKTERI DAN ANTI-UV BEBERAPA ASCIDIAN DARI PERAIRAN PANGALISANG BUNAKEN Macpal, Yuliana; Warouw, Veibe; Sumilat, Deiske A; Paulus, James J.H.; Rumampuk, Natalie D.C.; Kreckhoff, Reni L.
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.26019

Abstract

Ascidians are sesile marine invertebrates that have bioactive compounds such as antibacterial and anti-UV. The purpose of this study is to determine the antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and to test the anti-UV activity of the ascidian water fraction. Antibacterial activity test was carried out using the diffusion method (disc diffusion Kirby & Bauer) and the water fraction of ascidian that showing antibacterial activity tested in a UV spectrophotometer to see the anti-UV activity. Results of the study were obtained 4 types of ascidians extracted from partitioned into water fraction, n-hexane fraction, methanol fraction. All three fractions were tested for antibacterial activity and the result showed that there were antibacterial activities of ascidian extract Clavelina sp. against both test bacteria with inhibition of S. aureus and E. coli with strong categories. Ascidian Phlebobranch sp. showed the presence of antibacterial activity with inhibition of S. aureus and E. coli with very strong categories (16,6 mm). extract Eudistoma sp. showed the presence of antibacterial activity with inhibition S. aureus and E. coli in the medium category (9 mm). The water fraction found active in antibacterial testing is then tested using a UV spectrophotometer for anti UV testing, the result show that water fraction of the four ascidians can absorb UV-B (290-320nm) and UV-A ( 320-400nm).Keywords : Ascidian, Antibacterial, Partition, Anti- UV.
Accumulation of heavy metals (As, Cd, Pb, Hg) on brown algae, Padina australis, cultivated in Kima Bajo Waters, North Minahasa Regency Hidayat, Sarif; Mantiri, Desy M.H.; Paulus, James J.H.; Lasut, Markus T.; Rumampuk, Natalie D.C.; Undap, Suzanne; Sumilat, Deiske A.
AQUATIC SCIENCE & MANAGEMENT Vol 9, No 1 (2021): APRIL
Publisher : AQUATIC SCIENCE & MANAGEMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.9.1.2021.32470

Abstract

Although heavy metals have relatively low levels in water column and sediments, they will not be degraded and can even be absorbed and accumulated by marine algae. Research on accumulation of heavy metals on algae was done in Kima Bajo Waters, North Minahasa Regency. The research objective was to evaluate the accumulation of four heavy metals, i.e. arsenic (As), cadmium (Cd), plumbum (Pb), and mercury (Hg), on brown macroalgae Padina australis. The alga was introduced and cultivated in the research area using bottom method. The same method was also used in the experiment. One-week acclimatization process was done prior the experiment. Sampling of alga’s thallus was carried out every two weeks to measure the concentration of metals. The experiment was terminated after six weeks. Bottom sediment was also collected at the beginning of the experiment to measure the metal concentration. The results showed that the heavy metals (As, Cd, Pb, and Hg) were accumulated in the algae. The highest concentration of Cd, Pb, and Hg accumulated in the algae was 0.15 mg/kg wet weight, 3.5 mg/kg wet weight, and 0.009 mg/kg wet weight, respectively. All the concentrations were higher than the initial concentration of each metal. Accumulation of As was also occurred with the highest concentration (1,9 mg/kg wet weigh) occurred at fourth weeks; however, it was lower than the initial concentration. The accumulation varied according to type of metals and time; accumulation of Cd, Pb, and Hg were occurred on second weeks of cultivations and As on the fourth weeks. It can be concluded that cultivated alga can accumulate heavy metals.Indonesian title: Akumulasi logam berat (As, Cd, Pb, Hg) pada alga coklat, Padina australis, yang dibudidaya di Perairan Kima Bajo, Kabupaten Minahasa Utara
Co-Authors Deiske Adeliene Sumilat, Deiske Adeliene Kreckhoff, Reni L. Macpal, Yuliana Mantiri, Desy M. H Markus T. Lasut Natalie D Rumampuk Sarif Hidayat, Sarif Suzanne L Undap Veibe Warouw