Kreckhoff, Reni L.
Staf Pengajar Pada Program Studi Budidaya Perairan FPIK UNSRAT

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Administrasi oral imunostimulan ragi roti (Saccharomyces cerevisiae) untuk meningkatkan pertumbuhan ikan mas (Cyprinus Carpio L.) Razak, Atiek P.; Kreckhoff, Reni L.; Watung, Juliaan Ch.
e-Journal BUDIDAYA PERAIRAN Vol 5, No 2 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.5.2.2017.16637

Abstract

The research aimed to evaluate the effect of baker’s yeast as immunostimulant on the growth of carp.  Fish was obtained from Board of Freshwater Aquaculture at Tatelu, North Sulawesi Province.  Before running the experiment, the fish was acclimatized for one week in five concrete tanks measuring 2x1x1 m3 each. During acclimatization, fish was fed commercial pellet at 5%/body weight/day, twice a day.  After acclimatizationm, the fish was distributed in 15 aquaria with a density of 25 individuals per aquarium.  The research used Complete Randomized Design with five treatments namely 0, 5, 10, 15, and 20 g yeast/kg of pellet, each with three replications.  Fish was fed with treatment pellets for three weeks consecutively at a dose of 5%/body weight/day, twice a day at 08.00 am and 16.00 pm. Growth of fish was measured at the end of feeding period.  The result showed that the addition of baker’s yeast cells into fish feed significantly influenced fish growth. The best growth was achieved in fish fed pellet supplemented with 5 g yeast/kg of pellet.Keywords : Cyprinus carpio, Saccharomyces cerevisiae, immunostimulant, growth
Suplementasi bakteri probiotik meningkatkan performa pertumbuhan dan efisiensi pakan benih ikan mas (Cyprinus carpio). Mudeng, Cindy C.; Manoppo, Henky; Lantu, Sartje; Kreckhoff, Reni L.; Tumbol, Reiny A.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27222

Abstract

Probiotic from carp intestines and its effects on increasing growth performance and feed efficiency had been investigated. Fish used were carp fingerlings weighing 2.33 g  in average. The fish were taken from the Tatelu Freshwater Aquaculture Fisheries Center.  Probiotics used were isolated from the intestines of carp. Before running the experiment, the fish were acclimatized for 10 days under laboratory conditions. During acclimatization the fish were fed commercial pellets without the addition of probiotics. The dose of feeding was 5% /bw/day, 2 times a day at 09:00 am and 16:00 pm. Furthermore, fish are stocked in 15 aquariums each measuring 60x40x40cm3 with a density of 20 fish/aquarium. Each aquarium was equipped with aeration and water pumps for recirculation. Uneaten feed and feces were removed through siphoning. Water exchange was done every 2-3 days.  After acclimatization, the fish were fed with pellet supplemented with probiotic at  concentration of A: without addition of probiotic; B: 1 x 106 cfu /mL; C: 1 x 107 cfu / mL; D: 1 x 108 cfu / mL; E: 1 x 109 cfu/mL. The fish were fed twice a day with feeding dose of 5% /bw/ day, for 21 days. Data collected consisted of daily growth rates, growth rates, feed efficiency and feed conversion ratio. The results of research showed that the addition of probiotics in feed had a very significant effect on growth, feed efficiency, and feed conversion ratio (P <0.01) with the best results achieved in administering probiotics as much as 1 x 108 cfu / mL. In conclusion, probiotic bacteria originating from the intestines of carp can be used to improve the growth performance of the fish itself.
Penerapan teknologi rekayasa konstruksi wadah budi daya rumput laut bagi masyarakat di Pulau Nain Ngangi, Edwin L. A.; Kreckhoff, Reni L.; Gerung, Grevo S.
e-Journal BUDIDAYA PERAIRAN Vol 7, No 1 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.7.1.2019.28065

Abstract

The Higher Education Community Empowerment Program (PPMUPT) activity aimed to disseminate research results on design engineering and construction materials for verticultural cultivation of seaweed; Seed quality from seaweed cultivation centers in North Sulawesi; and Co-cultivation of seaweed and sea tilapia. The implementation partnered with the Nain Princess and Mekar Laut Groups, and was supported by the Nain Village Government. The three partners expressed their willingness and ability to work together in formulating and finding solutions to problems, as well as planning the implementation stages and all of these activities. In addition, partners continue to contribute throughout the activity. Formulation of problems with partners, are: Is there a technology that can minimize disease attacks on seaweed. The solution is in the form of: a. Engineering design and construction material for cultivation containers, b. Verticultural cultivation of engineering methods. The method applied was the classical and individual approach in the form of class lectures, demonstrations, and practices about the innovation of seaweed cultivation technology. The outputs of the PPMUPT activities were productive economic partners that have increased knowledge, skills, quality, quantity, and types of products. Participants were principally satisfied with this PPMUPT activity, and even 92.86% stated that they were very satisfied in participating in PPMUPT activities regarding the use of strings as ris ropes with seaweed verticulture methods.
Financial analysis of seaweed, Kappaphycus alvarezii, farming business toward farmer’s income development in Minahasa Penninsula, North Sulawesi, Indonesia Kreckhoff, Reni L.; Ngangi, Edwin L. A.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 3 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.3.2018.28066

Abstract

This study was carried out in the present farming sites of Kappaphycus alvarezii in Minahasa penninsula, Jayakarsa village of North Minahasa, Sondaken of South Minahasa, Buku Tengah of  Southeast Minahasa, and Tumpaan of Minahasa Regencies. It was aimed to know the farming system and financially analyze whether the farming business is feasible or not.  Data were collected through observations, questioneers and interviews.  Literature reviews and financial analyses were also done to know the revenue, the feasibility of  seaweed, K. alvarezii, farming business and its development. The farming system in Minahasa penninsula generally used longline method.  It is used because of being resilient to strong wind, cheap, and easily implemented and long lived so that the line could be repeatedly used up to  4-5 years.  Financial analyses found that among 4 study sites, the highest B/C ratio was recorded in North Minahasa, 6.06, followed by South Minahasa, 2.75, then Southeast Minahasa and Minahasa, 1.67, and BEP values were also different among regencies.  Furthermore, NPV, IRR, ROI and PP of all study sites belonged to feasible criteria for development.  Different conditions among regencies resulted from different geographic conditions affected the planting system.
Respon imun benih ikan mas, Cyprinus carpio, yang diberi pakan probiotik Lactobacillus sp. dengan konsentrasi berbeda Pangalila, Novelia; Manoppo, Henky; Tumbol, Reiny A.; Lumenta, Cyska; Kreckhoff, Reni L.; Warouw, Veibe
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27492

Abstract

The research aimed to isolate probiotic bacteria from catfish intestine and assign the concentration of probiotic bacteria which effectively increase the activity of phagocytosis and total leukocytes of carp fingerlings. The fish was taken from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. Fish (average initial weight of 2.27 g) were acclimated for one week in 15 aquaria with a density of 25 fish/aquarium. One week after acclimatization, fish were fed with food added with probiotics with different concentrations, namely 1x106, 1x107, 1x108, 1x109 cfu/mL for four weeks. The dose of feed was 5% /body weight/day and feeding time was at 08.00 and 17.00. Data collected were phagocytosis and total leukocyte count. The results showed that supplementation of probiotic significantly increased phagocytic activity and total leukocytes (p<0.01). The best phagocytic activity and total leukocytes were achived in fish fed diet added with probiotics 1x108 cfu/mL. It can be concluded that supplementation of  probiotic onto feed can increase phagocytic activity and total leukocytes of carp.
Konsentrasi Hambatan Minimum ekstrak Portulaca grandiflora terhadap penyakit Motile Aeromonad Septicaemia Lauluw, Dafid; Kreckhoff, Reni L.; Longdong, Sammy N.J.; Mantiri, Desy M.; Watung, Juliaan Ch.; Tumbol, Reiny A.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20659

Abstract

The objective of this study was to determine the minimum concentration of extract in inhibiting motile motile aeromonad septicaemia. The material used was the stems and leaves of the plant P. grandiflora as much as 10 g. The crushed simplicia was then macerated with aquades with a ratio of 1:10,  then filtered with filter paper and, put in an Erlenmeyer, and evaporated in an incubator at temperature of 30°C. The sensitivity test was performed by placing a paper disc containing extract on the agar medium that had been scratched with bacterial colonies. Clear zone formed around the paper disc was compared with standard classification table for clarifying the inhibition level.  The results showed that stem and leaf extract had inhibition zone against Aeromonas hydrophila bacteria that caused motile aeromonad septicaemia. The best result was obtain at concentration of extract 3.25 mg / L) with an average diameter of 22.67 mm and for leaf extract had a moderate to strong zone for all treatments. These proved that stem and leaf extract of P. grandiflora plant had antibacterial activity and can be used as control of MAS disease caused by A. hydrophila bacteria. Keywords:  Portulaca grandiflora plant, motile aeromonad septicemia,  minimum       inbibition concentration
Efikasi ekstrak tanaman obat Binahong Anredera cordifolia (Ten) Steenis untuk meningkatkan pertumbuhan ikan Nila Oreochromis niloticus Wantah, Monika M.; Longdong, Sammy N.J.; Kreckhoff, Reni L.; Mantiri, Desy M.H.; Tumbol, Reiny A.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20502

Abstract

The aim of this research was to study the effect of Binahong extract on the growth of Nile tilapia. A number of 225 fish measuring 10-12 cm in length with an average weight of 15 g was obtained from Fishery Development Center and Training, Tateli. The fish were put  in oxygenated plastic bags and transported to Laboratory of Aquaculture Technology. The fish was kept in aquarium equipped with an aerator with a density of 15 fish per aquaria. The test material used was the stem of Binahong plant stem, macerated with alcohol 70% for 3 days. The extract was then filtered with whatman filter paper and extract was concentrated using a Rotary Evaporator. The extract was further diluted to obtain  concentrations of 12.5, 25, 50, and 100 mg / mL Binahong  extract as treatment was given to the fish after mixed first into the pellet. The fish was fed with treatment feed for 30 days. Control feed was not supplemented with binahong extract. Growth data was measured at day 30 after treatment. The results showed that the extract of binahong had significant effect on fish growth (p <0.01) with the best result achieved at concentration of 100 mg/mL. In conclusion, supplementation of  binahong extract into fish feed could improve growth of  fish in which the best growth was obtained in fish fed diet containing 100 mg/mL of binahong extract.Keywords: Medicinal plants, tilapia, Anredera cordifolia, growth, maceration
Pertumbuhan benih ikan mas, Cyprinus carpio, yang diberi pakan dengan dosis berbeda pada kolam pekarangan dengan sistim resirkulasi Jasansong, Kalfianus; Salindeho, Indra R. N.; Kreckhoff, Reni L.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27198

Abstract

The research was aimed to find out the optimum dose of feeding regime             to ensure the maximum growth of carp-seeds, Cyprinus carpio, reared in backyard pond with recirculation system. The experiment was carried out in Randomized Block Design with 3 different dose of feeding regimes as treatments, which were 3%, 4% and 5% of the total body weight per day.  Each treatment was triplicate and each repetition functioned as group of fish with different weight.  There were 3 groups which were, fish weighing 1.5–2.4 gram (Group-1), fish weighing 2.5 – 3.4 gram (Group-2) and fish weighing 3.5 – 4.8 gram (Group-3).  Each experimental unit was composed of 8 tested fish, placed in a netting-cage bag measuring 60×30×60 cm (l×w×h) with a mesh-size of 4 mm. Each group was composed of 24 tested fish, hence there were 72 tested fish in total.  Fish were fed twice a day at 07:00 and 17:00.  Tested fish were weighed every week, and the fish weight data were converted into FCR, absolute, relative and daily growth rate. Data were statistically analyzed using JMP statistic-program.  The results showed that, there was no significant difference in absolute, relative and daily growth rate among fish fed 3%, 4% and 5% of total body weight per day.   The best FCR was performed by fish fed 3% of total body weight per day.   Hence, this experiment result suggests that, carp reared in backyard pond with recirculation system should be fed with a dose of 3% of the total body weight each day, as fish might grow well and at the same time the amount of uneaten food, feces and metabolic wastes is minimized at this dose of feeding.
AKTIVITAS ANTIBAKTERI DAN ANTI-UV BEBERAPA ASCIDIAN DARI PERAIRAN PANGALISANG BUNAKEN Macpal, Yuliana; Warouw, Veibe; Sumilat, Deiske A; Paulus, James J.H.; Rumampuk, Natalie D.C.; Kreckhoff, Reni L.
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.26019

Abstract

Ascidians are sesile marine invertebrates that have bioactive compounds such as antibacterial and anti-UV. The purpose of this study is to determine the antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and to test the anti-UV activity of the ascidian water fraction. Antibacterial activity test was carried out using the diffusion method (disc diffusion Kirby & Bauer) and the water fraction of ascidian that showing antibacterial activity tested in a UV spectrophotometer to see the anti-UV activity. Results of the study were obtained 4 types of ascidians extracted from partitioned into water fraction, n-hexane fraction, methanol fraction. All three fractions were tested for antibacterial activity and the result showed that there were antibacterial activities of ascidian extract Clavelina sp. against both test bacteria with inhibition of S. aureus and E. coli with strong categories. Ascidian Phlebobranch sp. showed the presence of antibacterial activity with inhibition of S. aureus and E. coli with very strong categories (16,6 mm). extract Eudistoma sp. showed the presence of antibacterial activity with inhibition S. aureus and E. coli in the medium category (9 mm). The water fraction found active in antibacterial testing is then tested using a UV spectrophotometer for anti UV testing, the result show that water fraction of the four ascidians can absorb UV-B (290-320nm) and UV-A ( 320-400nm).Keywords : Ascidian, Antibacterial, Partition, Anti- UV.
AMPLIFIKASI GEN 16S-rRNA BAKTERI EPIFIT PADA ALGA MERAH Kappaphycus alvarezii Rico Taareluan, Rico; Wantania, Letha L; Ginting, Elvy L; Mangindaan, Remy E.P.; Kumampung, Deislie R.H.; Kreckhoff, Reni Lusia; Wullur, Stnly
JURNAL PESISIR DAN LAUT TROPIS Vol 8, No 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27696

Abstract

Bacteria are microscopic organism found living in marine algae.  So far, species of bacteria in marine algae are not well known.  In this study, epiphytic bacteria in algal species of Kappaphycus alvarezii (red algae) were isolated to amplify their 16S-rRNA gene. Sample K.alvarezii was collected from the island of Nain.  The isolated epiphytic bacteria from the red algae K.alvarezii were grown in Nutrient Broth (NB) media.  DNA extraction was carried out using InnuPREP DNA Mini Kit.  16SrRNA genes was performed using primer pair of 8F and 1492R. Two different character of epiphytic bacteria were successfully isolated from K. alvarezii.  16S-rRNA genes from the two isolates was successfully amplified, indicated by the presence of DNA band in each sample gel electrophoreses at around 1500 bp.Keywords : Amplification, 16S-rRNA Genes, Kappapchycus alvarezii, Bacterial, Epiphytic  Abstrak Bakteri adalah organisme mikroskopis yang ditemukan hidup di alga laut. Sejauh ini, spesies bakteri di alga laut belum dikenal. Dalam penelitian ini, bakteri epifit pada spesies alga Kappaphycus alvarezii (alga merah) diisolasi untuk memperkuat gen 16S-rRNA mereka. Sampel K.alvarezii dikumpulkan dari pulau Nain. Bakteri epifit terisolasi dari alga merah K.alvarezii ditanam dalam media Nutrient Broth (NB). Ekstraksi DNA dilakukan dengan menggunakan InnuPREP DNA Mini Kit. Gen 16S-rRNA dilakukan dengan menggunakan pasangan primer 8F dan 1492R. Dua karakter bakteri epifit yang berbeda berhasil diisolasi dari K. alvarezii. Gen 16S-rRNA dari dua isolat berhasil diamplifikasi, ditunjukkan oleh adanya pita DNA pada setiap sampel gel elektroforesis sekitar 1500 bp. Kata Kunci: Amplifikasi, gen 16S-rRNA, Kappapycus alvarezii, Bakteri, Epifit
Co-Authors Adellin Dwiny Conchita Adnan Wantasen Alex D. Kambey Ali Djamhuri Aylifia S. Somba Billy Theodorus Wagey Cyska Lumenta Cyska Lumenta Dahlia Malau Datangmanis, Adieng A. R. Deiske Adeliene Sumilat, Deiske Adeliene Deiske Sumilat Dhebby Purba Diane J. Kusen Edwin L. A. Ngangi Elvy Ginting Elvy L Ginting Fitje Losung Fransine B. Manginsela Ginting, Elvi L. Grevo S Gerung Hariyani Sambali Henky Manoppo Henky Manoppo Henneke Pangkey Indra R. N. Salindeho Inneke F. M Rumengan Jasansong, Kalfianus Jhonly Solang Joppy Mudeng Juliaan Ch. Watung Kumampung, Deislie R.H. Kurniawan, Putra M. Lauluw, Dafid Leyda H. Mokoginta Lintang, Rosita AJ Macpal, Yuliana Mamuko, Nelsi Mantiri, Desy M. H Massora Dudung Maweikere, Fabiola C. Monijung, Revol D. Montolalu, Gianfranco Mudeng, Cindy C. Natalie D Rumampuk Nego E. Bataragoa Ngangi, Edwin L. A. Novie P.L. Pangemanan Ockstan J. Kalesaran Pangalila, Novelia Pangemanan, Novie P.L. Paulus, James J.H. Prislawati Paputungan Razak, Atiek P. Reiny A. Tumbol Reiny A. Tumbol Reiny A. Tumbol Reiny A. Tumbol Remy E. P Mangindaan Rico Taareluan, Rico Rizald Rompas Rompas, Rizald M. Sambali, Hariyani Sambali, Haryani Sammy N. J. Longdong Sammy N. J. Longdong Sammy N.J. Longdong Sanggaria, Ayu W. Sartje Lantu Sartje Lantu Selvyane Bukasiang Shifa A. Schram Sinaga, Chintya R. Sinubu, Wulan V. Suria Darwisito, Suria Suzanne L Undap Suzanne L. Undap Tindage, Tantri W. Tununu, Riky R. Umasugi, Aisin Undi, Chintia S. Veibe Warouw Veibe Warouw Wantah, Monika M. Wantania, Letha L Winda M. Mingkid Winda M. Mingkid Winda M. Mingkid Winda M. Mingkid, Winda M. Wullur, Stnly