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All Journal Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY BIOEDUKASI Dinamisia: Jurnal Pengabdian Kepada Masyarakat Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Pengabdian dan Peningkatan Mutu Masyarakat (JANAYU) Journal of Comprehensive Science (JCS) Prosiding University Research Colloquium International Journal of Health Science and Technology Hasil Karya Aisyiyah Untuk Indonesia

Annisa Khumaira

Universitas Aisyiyah Yogyakarta

Author-ID : 1556882

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Other

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The design of Indonesian SARS‐CoV‐2 primers based on phylogenomic analysis of the SARS‐CoV‐2 clades Tsania Taskia Nabila; Ata Rofita Wasiati; Afif Pranaya Jati; Annisa Khumaira
Indonesian Journal of Biotechnology Vol 27, No 1 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.66854

Molecular detection needs to be augmented for COVID‐19 detection in Indonesia using the PCR method with primer‐based gene analysis. This is necessary because the RNA of the SARS‐CoV‐2 virus, the causative infectious agent of the pandemic, has been mutated. Therefore, this study aimed to develop a primer design for determining SARS‐CoV‐2 clades in Indonesia using phylogenomic analysis. Data were obtained from 38 GISAID (Global Initiative on Sharing All Influenza Data) viruses and the relationships were analyzed using maximum likelihood (ML) phylogenomic analysis with a substitution model of generalized time‐reversible (GTR) to construct the tree topology. The results showed that the five types of SARS‐CoVs‐2 clades in Indonesia were L, G, GH, GR, and O. It also indicated that the GH region had the highest rate of clade at 50%, with the S clade affecting its formation. Furthermore, the genome sequences of the GH type used to design its primer were based on three genes, namely RdRp, S, and N. The RdRp and N genes were found to be conserved and hardy mutants, while the S gene occurred repeatedly. Several previous studies have stated that the designed primers produced missense mutations compared to another in silico. Therefore, three sets of primers were achieved from the GC contents and clamps, Tm range, and structural secondary indicator standards.

Effects of Peel Extract from Citrus reticulata and Hesperidin, A Citrus Flavonoid, on Macrophage Cell Line Muthi’ Ikawati; Inna Armandari; Annisa Khumaira; Yogi Ertanto
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm30iss4pp260

The extract of Citrus reticulata has been studied for its biological activities, due to its citrus flavonoid content. The extract and its flavonoid compounds exhibit growth inhibition properties in several cancer cell lines and in vivo models. Conversely, the extract can also induce cell proliferation and angiogenesis, and shows estrogenic effects, in vitro and in vivo. Because of the contrasting effects that depend on the concentration or dosage, the precise action of the extract and its flavonoids need to be elucidated in various cell types. The objective of this study is to evaluate the effect of Citrus reticulata peel extract (Citrus extract) and hesperidin, a citrus flavonoid, on the modulation of cell proliferation in the RAW 264.7 macrophage cell line. Cell viability under Citrus extract or hesperidin treatment was assessed by using the MTT assay. The expression of interleukin-10 (IL-10), an anti-inflammatory cytokine, modulated by Citrus extract was also examined by immunostaining. Low concentrations of Citrus extract at 1 and 100 μg/mL were able to induce cell proliferation, though not significantly, as shown by cell viability of 138 and 114%, respectively. At higher concentrations of 500, 750, and 1000 μg/mL, Citrus extract decreased cell viability significantly by up to 64, 46, and 36%, respectively. Accordingly, hesperidin at low (3.1 μg/mL−61.1 μg/mL) or high (152.6 μg/mL−305.3 μg/mL) concentrations increased or reduced cell viability significantly by up to 116−136% or 10−61%, respectively. The value of the 50% inhibitory concentration (IC50) of Citrus extract was more than three times higher (756 μg/mL) than that of hesperidin (203 μg/mL = 332 μM). Additionally, 250 μg/mL of Citrus extract was able to induce IL-10 expression compared with the control. These results demonstrate that Citrus extract and hesperidin exert a biphasic effect on macrophage cells. The future development of Citrus extract as a co-chemotherapeutic, anticancer, or immunomodulatory agent should include careful consideration of its biphasic effect on each cell type.

POTENCY OF ANTICANCER COMPOUNDS FROM THE InaCC LIPI COLLECTION Actinomycetes WERE ANALYZED USING antiSMASH Annisa Khumaira; Tsania Taskia Nabila
BIOEDUKASI Vol 19 No 1 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i1.22747

Every year cancer cases have always increased. According to WHO data, in 2020, there were 19.2 million cases of cancer. Cancer treatment is still developing by exploring medicinal compounds that have a minimal risk to healthy cells but can still kill cancer cells. Secondary metabolites from the phylum Actinomycetes are often explored to determine their usefulness, one of which is an anticancer compound. The InaCC project from LIPI has several collections of Actinomycetes that have never been explored, mainly novel species obtained in the 2011-2016 timeframe. The purpose of this study was to analyze the genomic sequences of the InaCC Actinomycetes collection using antiSMASH to determine the potential of the compounds for anticancer. First, the genome sequence data search was carried out through the NCBI gene bank, then analyzed the sequences using antiSMASH 5.0, after searching for the compounds' potential through publication search using the ChEMBL Database and Google Scholar. In the NCBI database, all bacterial phylum Actinomycetes InaCC 2011-2016 have not found any genomic sequence data to use bacteria with the closest relationship for antiSMASH analysis. The closest relatives' analysis results showed that BGC compounds that can have anticancer properties in the bacterial collection of the phylum Actinomycetes InaCC 2011-2016 include terpenes butyrolactol, furans, alkyresorcinol, desferroxamine, and siderophore. These results indicate that the phylum Actinomycetes bacteria could have BGC anticancer compounds and could be developed to treat cancer. The analysis of this research needs to be confirmed again if each bacterium's genomic sequence is complete.

Deteksi Red Sea Bream Iridovirus (RSIV) dengan Teknik PCR pada Benih Ikan Gurame (Osphronemus gouramy) di Stasiun KIPM Yogyakarta Hamiyawati Qoimatu Dini Alfaruqi; Sutopo Aris Wibowo; Annisa Khumaira
Prosiding University Research Colloquium Proceeding of The 11th University Research Colloquium 2020: Bidang Sains dan Teknologi
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (792.374 KB)

Kebutuhan benih gurame semakin meningkat sejalan dengan pengembangan usaha budidaya gurame yang semakin luas. Hal tersebut berdampak pada peningkatan lalulintas perdagangan benih gurame dan Stasiun KIPM adalah lembaga yang berwenang untuk melakukan pengawasan serta pencegahan apabila terdapat HPI/HPIK. Red sea bream iridovirus (RSIV) merupakan salah satu agen penyakit dari golongan virus yang menyebabkan kematian massal pada budidaya benih gurame. Tujuan dari penelitian ini adalah untuk mendeteksi RSIV pada benih gurame dengan teknik PCR. Metode deteksi menggunakan tahapan molekuler yang mengacu pada metode The Office International des Epizooties (OIE). Amplifikasi DNA virus menggunakan primer spesifik RSIV yaitu primer forward 1F (5’-CTCAACACTCTGGCTCATC-3’) dan primer reverse 1R (5’-GCACCAACACATCTCCTATC-3’) dengan amplikon yang dihasilkan sebesar 570 bp. Produk PCR divisualisasi dengan elektroforesis gel agarose 1%. Hasil penelitian menunjukkan bahwa ketiga sampel benih gurame negatif RSIV. Hal ini ditandai dengan tidak terbentuknya pita DNA pada gel elektroforesis, sehingga benih gurame tersebut aman jika dilalulintaskan.

The Detection of Genes Encoding Enzyme Fructosyltransferase The Exopolysaccharide (EPS) Produce Potential Probiotic Candidates For Breast Milk (ASI) Afifah Nurul Falih; Nosa Septiana Anindita; Arif Bimantara; Annisa Khumaira
International Journal of Health Science and Technology Vol 4, No 1 (2022): July
Publisher : Universitas 'Aisyiyah Yogyakarta

Bacteria is one of the microorganisms that we can easily find in nature. Bacteria can be classified into benefical bacteria and harmful bacteria. One of the bacteria classified as benefical bacteria is lactic acid bacteria (LAB). One of the bacteria included in LAB is probiotic bacteria. Probiotic bacteria can be isolated from several sources, one of which is breast milk. The functionality of probiotics is seen by the production of exopolysaccharides (EPS). EPS is produced through the expression of the fructosyltransferase (ftf) gene. The samples used in this study were 16 isolates of candidate probiotic bacteria from breast milk. The stages of this research included reculture of probiotic isolates, isolation of bacterial genomic DNA, amplification of isolates with 16s rRNA primers, amplification of isolates with primary lactic acid bacteria (LAB) and amplification of isolates with specific primers of ftf gene. The results showed that 10 of 16 probiotic bacterial isolates had the ftf gene indicated by the presence of DNA bands on the electrophoregram with a size 58 bp.

Bacillus sp. Effectivity test as a growth supressor agent from antrachnose disease caused by Colletotrichum sp. on cayenne pepper plant (Capsicum frutescens L.) nita widyaningsih; Wisnu Adhi Susila; Annisa Khumaira
International Journal of Health Science and Technology Vol 3, No 1 (2021): July
Publisher : Universitas 'Aisyiyah Yogyakarta

cayenne pepper plant (Capsicum frutescens L.) is one of the popular vegetables comodity in Indonesia. cayenne pepper plant used as main ingridients for kitchen’s need, sauce industry, chili powder, instant noodle, and pharmaceutical industry. The demand for cayenne pepper plant in Indonesia is considerably high, about 4kg/capita/year. Antrachnose diseease caused by Colletotrichum sp is on of the major problem in cayenne pepper plant cultivation. Colletotrichum sp is from Nectrioidaceae family that has plenty of aservulus beneath the cuticule or on the surface, the diameter is up to 10 nm, black colored and many setae. Several bacteria has antagonist feature either around the root, or endophytes with the roots. Bacillus sp has antagonist feature is consider to treat the Antrachnose disease. Bacillus sp is a marine bactreia that can produce antibiotic to fight pathogen. However, from this study there are two different antagonist activites between two isolate due to different chytinolytic activitiy between the two isolate.

The effect of adding casein hydrolysate as a protein source in the culture of the bacteria Paenibacillus polymyxa at wilker office of food and horticultural plants protection (PTPH) Bojonegoro Nanda Putri Insan Khasana; Miwing Nurul W; Annisa Khumaira
International Journal of Health Science and Technology Vol 3, No 2 (2021): November
Publisher : Universitas 'Aisyiyah Yogyakarta

Agencia (Biological Control Agents) are currently widely used to control pests and diseases or plant-disturbing organisms. One of the biological agents that has many benefits for controlling several types of diseases in both food crops and horticulture is Paenibacillus polymyxa. These bacteria are beneficial in nitrogen fixation, promotion of plant growth, solubilization of soil phosphorus, and production of exopolysaccharides, hydrolytic enzymes, antibiotics, and cytokinins. Paenibacillus polymyxa also produces polymyxin antibiotics and it is known that these bacteria contain the hormone gibberellins. Casein hydrolyzate is one of the growth media that can be used for the growth of microorganisms. The media is a complex mixture of 18 amino acids, vitamins, calcium, phosphate, and several microelements which results in their high price. Casein hydrolyzate is known to be more effective for plant tissue culture. Therefore, the purpose of this study was to determine whether the amino acid content in casein hydrolyzate could affect the growth of bacteria Paenibacillus polymyxa. The simple medium used in this study was soybean boiled water. Soybean as an alternative medium for protein sources to substitute beef extract, beef extract, and bacto peptone for the growth of bacteria Paenibacillus polymyxa. Based on TPC (Total Plate Count) calculations with graded dilution in Paenibacillus polymyxa, it was found that there was no difference in the number of bacteria to the addition of casein. This shows that bacteria can grow optimally even though casein hydrolyzate is not given during the growth process.

Bio-Pack : Biodegradable Packaging Pati Singkong Sebagai Solusi Pencemaran Limbah Plastik Konvensional Dhea Sandra Fitriany; Salma Annaziha; Hiskia Sains Assajuly Syamsuddin; Annisa Khumaira
Journal of Comprehensive Science (JCS) Vol. 2 No. 1 (2023): Journal of Comprehensive Science (JCS)
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/jcs.v2i1.229

Masih banyak pelaku usaha yang menggunakan plastik konvensional sebagai kemasan makanan. Sampah plastik konvensional tentunya memiliki banyak dampak yang akan menimbulkan permasalahan lingkungan. Termasuk pada fauna yang mengalami kematian karena mengkonsumsi plastik ini yang terbuang di alam. Oleh karena itu, kehadiran plastik biodegradable dapat mengatasi masalah ini, dan perlu untuk terus dikembangkan. Plastik biodegradable menggunakan bahan baku dari nabati produk pertanian terbarukan. Bioplastik memiliki sifat biodegradable, termoplastik, dan sifat fleksibel. Selain bahan utama, plasticizer dan struktural bahan penguat diperlukan untuk menghasilkan plastik biodegradable dalam inovasi ini. Tahap produksi dalam pembuatan plastik ini meliputi pencampuran, pemanasan, dan pencetakan. Berdasarkan komponennya, komposisi yang dianggap berhasil diperoleh perbandingan 3:4:3 untuk bahan cuka, gliserol, dan kitosan. Bioplastik kemudian dibentuk menjadi standing pouch dengan sealer. Waktu optimal pada masa degradasi plastik ini kurang lebih selama 7 hari. Plastik biodegradable akan menjadi peluang untuk mengurangi dan menggantikan plastik konvensional di kemasan makanan yang dapat merusak lingkungan.

The influence of variations in starter concentration Lactobacillus plantarum AS4 indigenous from human breast milk to the characteristics of fermented milk Nosa Septiana Anindita; Annisa Khumaira
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 6, No 1 (2023)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2023.006.01.3

The fermentation of milk by Lactobacillus plantarum AS4 can be classified as a functional or nutraceutical food. Lactobacillus plantarum AS4 is a group of Lactic Acid Bacteria (LAB) isolated from human breast milk, which has the ability as a probiotic in vitro. This research aimed to determine the effect of Lactobacillus plantarum AS4 starter concentration on the characteristics of fermented milk produced. Variations concentration starter used were 0, 2, 4, 6, and 8% with an incubation temperature of 37 °C for 24 hours. Fermentation parameters collected were probiotic viability, total bacteria, titrable acidity, lactose, protein, and fat content. The results showed that variations in concentration starter Lactobacillus plantarum strain AS4 significantly affected the characteristics of fermented milk based on the parameters collected. The viability of fermented milk probiotics increased in milk treated with an 8% starter concentration variation (9.91 ±0.02 log CFU/mL) and total bacteria (8.84 ±0.07 log CFU/mL) in the fermented milk product. The levels of lactic acid (1.66 ±0.06%) and protein (4.32±0.03%) were also increased in fermented milk products with a starter variation of 8%. Significantly, fermented milk products with concentrations starter 8% decreased the pH value (4.10 ±0.01), lactose content (2.33±0.01%), and fat content (2.30±0.05%).

Penyuluhan Menghadapi Era New Normal Covid – 19 dan Bisnis di Masa Less Contact Economy di Rumah Baca Komunitas Yogyakarta Annisa Khumaira; Fitri Maulidah Rahmawati
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 2 (2023): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v7i2.4870

Abstract Since Covid-19 was declared a global pandemic, many changes have occurred in people's social life. The need to maintain physical distance has made an impact in various fields, one of which is that there is a recession in various countries, and Indonesia gives a signal that it will experience this, indicated by the country's gross income of -5.32%. In dealing with this, the government has imposed a "new normal", namely the return of community activities by implementing health protocols. Rumah Baca Komunitas (RBK), as a literacy community in Yogyakarta, its members need counseling on health protocols that are implemented during the new normal and business strategies during the occurrence of social restrictions. The results of the evaluation show that the participants feel the impact of social restrictions from an economic perspective. According to the participants, the material presented was useful for the participants to be disciplined in implementing health protocols and always have income despite limited access.

Co-Authors Afif Pranaya Jati Afifah Nurul Falih Arif Bimantara Ata Rofita Wasiati Dhea Sandra Fitriany Dhea Sandra Fitriany Dinar Mindrati Fardhani Fitri Maulidah Rahmawati Fitria Zintya Sari Hamiyawati Qoimatu Dini Alfaruqi Hamiyawati Qoimatu Dini Alfaruqi Hiskia Sains Assajuly Syamsuddin Inna Armandari Khasana, Nanda Putri Insan Mariatil Kabatia Miwing Nurul W Muthi Ikawati Nanda Putri Insan Khasana nita widyaningsih Nosa Septiana Anindita Nurul W, Miwing Salma Annaziha Salma Annaziha Susila, Wisnu Adhi Sutopo Aris Wibowo Tsania Taskia Nabila Tsania Taskia Nabila widyaningsih, nita Wisnu Adhi Susila Yogi Ertanto

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