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Afifah Nurul Falih
Universitas 'Aisyiyah Yogyakarta
Humanities Engineering Health Professions Medicine & Pharmacology Nursing Public Health

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Identifikasi Bakteri Patogen Pada Olahan Pangan Saat Arus Mudik Lebaran Di Terminal Tirtonadi Surakarta Afifah Nurul Falih; Nosa Septiana Anindita; Emmanuel Kristanti
Jurnal Kesehatan Vol 14, No.1 (2021): Jurnal Kesehatan
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/jk.v14i1.11056

Abstract

Keamanan pangan pada olahan pangan yang diperjual belikan menjadi perhatian bagi masyarakat terutama akibat yang ditimbulkan. Oleh karena itu, perlu dilakukan tindakan pengawasan melalui identifikasi terkait keberadaan bakteri patogen pada olahan pangan. Tujuan dari penelitian ini adalah mengidentifikasi keberadaan bakteri patogen dalam olahan pangan selama mudik lebaran di Terminal Tirtonadi Surakarta. Metode identifikasi yang digunakan meliputi pengambilan dan pengkayaan sampel, uji konfirmasi, uji penegasan dan analisis data. Hasil yang didapatkan dari penelitian ini yaitu dari 11 sampel olahan pangan, semua sampel hasil yang diperoleh negatif (-) terhadap bakteri Salmonella sp, Vibrio cholera (V.cholera), Staphylococcus aureus (S. aureus), Bacillus cereus (B. Cereus) akan tetapi 6 sampel teridentifikasi positif (+) cemaran Eschericia coli (E.coli).  Sehingga, dapat disimpulkan bahwa dari beberapa jenis bakteri patogen sebagai indikator cemaran olahan bahan pangan, terdapat satu cemaran bakteri patogen yang positif ditemukan dalam 6 sampel olahan pangan  yaitu E.coli.Kata kunci : Bakteri patogen, Escherichia coli,  keamanan pangan. 
The Detection of Genes Encoding Enzyme Fructosyltransferase The Exopolysaccharide (EPS) Produce Potential Probiotic Candidates For Breast Milk (ASI) Afifah Nurul Falih; Nosa Septiana Anindita; Arif Bimantara; Annisa Khumaira
International Journal of Health Science and Technology Vol 4, No 1 (2022): July
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.796 KB) | DOI: 10.31101/ijhst.v4i1.2526

Abstract

Bacteria is one of the microorganisms that we can easily find in nature. Bacteria can be classified into benefical bacteria and harmful bacteria. One of the bacteria classified as benefical bacteria is lactic acid bacteria (LAB). One of the bacteria included in LAB is probiotic bacteria.  Probiotic bacteria can be isolated from several sources, one of which is breast milk. The functionality of probiotics is seen by the production of exopolysaccharides (EPS). EPS is produced through the expression of the fructosyltransferase (ftf) gene. The samples used in this study were 16 isolates of candidate probiotic bacteria from breast milk. The stages of this research included reculture of probiotic isolates, isolation of bacterial genomic DNA, amplification of isolates with 16s rRNA primers, amplification of isolates with primary lactic acid bacteria (LAB) and amplification of isolates with specific primers of ftf gene. The results showed that 10 of 16 probiotic bacterial isolates had the ftf gene indicated by the presence of DNA bands on the electrophoregram with a size 58 bp. 
Co-Authors Annisa Khumaira Arif Bimantara Emmanuel Kristanti Nosa Septiana Anindita