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All Journal HAYATI Journal of Biosciences El-Hayah : Jurnal Biologi Indonesian Journal of Biotechnology
Rohmawati, Uun
Laboratory Of Microbiology, Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Malang, Semarang No.5 Malang 65145, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Comparison of Secondary Metabolite Content of Pteris vittata L. in Baluran National Park and Malang and Its Effect on Environment Sulasmi, Eko Sri; Rohmawati, Uun; Amin, Achmad Makin
El-Hayah : Jurnal Biologi Vol 7, No 1 (2018): EL-HAYAH (VOL 7, NO 1 September 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i1.7240

Abstract

Fern is cosmopolitan plants which are almost scattered in all parts of the world, one of which is found in Baluran National Park and in Malang. The potential and benefits of these ferns are quite important for agriculture and medicine because of the chemical compounds they have, especially in Pteris vitatta L. This study aimed to analyze secondary metabolites contained in P. vittata L. in Baluran National Park, Situbondo and in Malang. The samples used were leaves and rhizome P. vittata extracted using methanol 96%, followed by a qualitative test of the content of alkaloids, flavonoids, terpenoids, polyphenols, tannins, saponins, using phytochemical screening methods with several reagents. The results showed that the leaves and Rhizome P. vittata L.. in Baluran National Park, Situbondo were positively containing secondary metabolites of terpenoids, polyphenols, tannins, saponins, and alkaloids (Dragendorf and Bouchardat reagents), whereas flavonoids were not present in all samples. However, the results of P. vitatta L. phytochemical screening around Malang State University positively contained flavonoids, polyphenols, terpenoids, alkaloids (Wagner and Dragendorf reagents). The difference in results from these two places is because the secondary metabolite content in plants is affected by stressful environmental conditions such as soil texture where it is grown or is affected by the precursors of the secondary metabolites of the metabolites.
Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis Achmad Rodiansyah; Ainul Fitria Mahmudah; Mastika Marisahani Ulfah; Uun Rohmawati; Dwi Listyorini; Eko Agus Suyono; Sitoresmi Prabaningtyas
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.136

Abstract

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.
Isolation and characterization of α ‐amylase encoding gene in Bacillus amyloliquefaciens PAS Achmad Rodiansyah; Sitoresmi Prabaningtyas; Mastika Marisahani Ulfah; Ainul Fitria Mahmuda; Uun Rohmawati
Indonesian Journal of Biotechnology Vol 26, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.61425

Abstract

Amylolytic bacteria are a source of amylase, which is an essential enzyme to support microalgae growth in the bioreactor for microalgae culture. In a previous study, the highest bacterial isolate to hydrolyze amylum (namely PAS) was successfully isolated from Ranu Pani, Indonesia, and it was identified as Bacillus amyloliquefaciens. That bacterial isolate (B. amyloliquefaciens PAS) also has been proven to accelerate Chlorella vulgaris growth in the mini bioreactor. This study aims to detect, isolate, and characterize the PAS’s α‐amylase encoding gene. This study was conducted with DNA extraction, amplification of α‐amylase gene with polymerase chain reaction (PCR) method with the specific primers, DNA sequencing, phylogenetic tree construction, and protein modeling. The result showed that α‐amylase was successfully detected in PAS bacterial isolate. The α‐amylase DNA fragment was obtained 1,468 bp and that translated sequence has an identity of about 98.3% compared to the B. amylolyquefaciens α‐amylase 3BH4 in the Protein Data Bank (PDB). The predicted 3D protein model of the PAS’s α‐amylase encoding gene has amino acid variations that predicted affect the protein’s structure in the small region. This research will be useful for further research to produce recombinant α‐amylase.
Co-Authors Achmad Rodiansyah Ainul Fitria Mahmuda Ainul Fitria Mahmudah Amin, Achmad Makin Dwi Listyorini Eko Agus Suyono Eko Sri Sulasmi Mastika Marisahani Ulfah Mastika Marisahani Ulfah Sitoresmi Prabaningtyas