Claim Missing Document
Check
Articles

Found 2 Documents
Search

Variasi Karakter Anatomis Talus Padina australis Hauck 1887 (Dictyotales, Phaeophycota) di Pantai Karang Tengah Kabupaten Cilacap Samiyarsih, Siti; Ats’tsaury, Moch Iqbal Sufyan; Insan, Achmad Ilalqisny; Fitrianto, Nur
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v9i4.28208

Abstract

ABSTRAK: Karakter anatomi dapat digunakan untuk menentukan produktivitas kandungan alginat rumput laut P. australis Hauck. Penelitian ini bertujuan mengetahui variasi karakter anatomis talus Padina australis Hauck. Pengambilan sampel dengan purposive random sampling di pantai Karang Tengah, Kabupaten Cilacap. Persiapan mikroskopis dengan metode parafin dengan safranin 1% dalam alkohol 70%. Parameter yang diamati meliputi ketebalan epidermis, ukuran sel medula, ketebalan talus, dan ukuran tetra sporangia. Pengamatan struktur anatomi secara deskriptif dengan membandingkan karakter anatomi P. australis Hauck pada garis konsentris. Data karakter anatomi dianalisis dengan Uji-t dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa variasi karakter anatomi talus dengan garis konsentris 5 memiliki ketebalan epidermis adaxial dan abaxial masing-masing adalah 20,05 μm dan 12,55 μm, sedangkan pada garis konsentris 7 memiliki ketebalan 28,33 μm dan 18 μm. Ketebalan talus dengan garis konsentris 5 dan 7 masing-masing adalah 99,44 μm, 114,77 μm. Diameter meduler dengan garis konsentris 5 dan 7 masing-masing adalah 37,88 μm, 45,5 μm. Diameter rata-rata tetra sporangia masing-masing dengan garis konsentris 5 dan 7 yaitu 25,66 μm, 35,66 μm. Talus P. australis Hauck berdasarkan garis konsentris 5 dan 7 memiliki perbedaan ukuran pada ketebalan epidermis adaxial, ketebalan epidermis abaxial, ketebalan talus, diameter meduler, dan diameter tetra sporangia. Implikasi penelitian ini sebagai database pada data dasar karakter anatomi P. australis yang dikaitkan denggan produktsi alginat dan data dasar taksonomi.  ABSTRACT: Anatomical character data can be used to determine the productivity of P. australis Hauck's seaweed alginate content. Each thallus has a thallus size and the number of varied concentric lines. The aim of the research is to determine the anatomical characteristics of the Padina australis Hauck thallus. Seaweed sampling by purposive random sampling technique in Karang Tengah beach, Cilacap regency. Preparation of microscopic by paraffin method with safranin 1% in 70% alcohol. Parameters observed included thickness of the epidermis, size of a medullary cell, the thickness of thallus, and the size of tetra sporangia. The anatomical character data was analyzed by t-Test with a 95% confidence level. The result of the research showed that the anatomical character of the thallus with concentric line 5 had a thickness of adaxial and abaxial epidermis respectively is 20.05 μm and 12.55 μm, whereas in concentric line 7 had a thickness of 28.33 μm and 18 μm. The thickness of the thallus with concentric lines 5 and 7 respectively is 99.44 μm, 114.77 μm. Medullary diameter with concentric lines 5 and 7 respectively is 37,88 μm, 45,5 μm. The average diameter of tetra sporangia with concentric lines 5 and 7 respectively that is 25,66 μm, 35,66 μm. Thallus of P. australis Hauck based on concentric lines 5 and 7 has differences of sizes on the adaxial epidermal thickness, thickness of the abaxial epidermis, the thickness of the thallus, medullary diameter, and diameter of tetra sporangia. The implications anatomical characters associated with alginate productivity and taxonomic baseline data.
Selection of Soybean (Glycine max) Germplasm Against Biotrophic Fungi Disease Based on Anatomical Resistance Samiyarsih, Siti; Pratiwi, Ade Yuanita Putri; Muljowati, Juni Safitri; Fitrianto, Nur
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25109

Abstract

The obstacle to increasing the soybean production is an infection of rust disease caused by the biotrophic fungus, Phakopsora pachyrhizi. The research objectives were to determine the anatomical resistance and the level of resistance of soybean cultivars against rust disease. The embedding method for observed leaf structural anatomy. The disease severity based on the method of International Working Group on the Soybean Rust (IWGSR) rating system.  The experiment was arranged as a Completely Randomized Design (CRD) with Factorial Pattern and five times repetition. The first factor was soybean cultivars, namely Gepak Kuning, Slamet, Tanggamus, and Wilis. The second factor was P. pachyrhizi inoculation with 0 uredospores/mL (uninoculated) and 104 uredospores/mL (inoculated). The results showed that the soybean cultivars that have thicker cuticle and epidermis, high trichomes and low stomatal density, and low of stomatal conductance have better anatomical resistance to leaf rust disease. Wilis and Slamet cultivars are resistant cultivars, indicated by disease intensity of 20% and 24.6%, respectively. While the Tanggamus is moderately resistant cultivar and Gepak Kuning is a susceptible cultivar, indicated by disease intensity of 56.5% and 85.3%, respectively. The novelty of selection soybean germplasm against biotrophic fungal disease are important and effectiveness in order to increase the crop productivity. These three soybean cultivars potentially serve as genetic sources to develop high yielding soybean cultivars and resistant to rust disease.