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All Journal Jurnal Planta Tropika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Scripta Biologica BioEksakta : Jurnal Ilmiah Biologi Unsoed JURPIKAT (Jurnal Pengabdian Kepada Masyarakat)

Juni Safitri Muljowati

Faculty Of Biology, University Of Jenderal Soedirman, Purwokerto, Indonesia

Author-ID : 609675

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Veterinary

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Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Cancer Cells Ekowati, Nuraeni; Mumpuni, Aris; Muljowati, Juni Safitri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7546

Pleurotus ostreatus is a common mushroom cultivated in Indonesia, and potential properties of bioactive compounds for medicinal mushroom. This study was aimed at obtaining P.ostreatus extract bioactive compounds potential in inhibiting the proliferation of cervical cancer cells (HeLa) and evaluating the HeLa cell proliferation kinetics and HeLa cell death mechanisms. The research was beneficial in making this product can be easily applied in a more controlled industrial scale. Anticancer activity test through a cytotoxic test using the MTT [3- (4,5-dimetiltiazol-2-yl) -2.5-diphenyl tertrazolium bromide], the kinetics proliferation of HeLa cells and HeLa cell death mechanism was performed. Linear regression analysis was used to analyze the data. Ethyl acetate extract of P. ostreatus isolated from Madiun showed the best results with IC 50 = 107.59 g / ml. HeLa cell proliferation kinetics analysis showed that the application of bioactive compounds 100 g / ml resulted in an increase of in death of HeLa cells along with length of incubation time. An important finding was that HeLa cells death by apoptosis was greater than by necrosis. In conclusion, the extracts of P. ostreatus has the potential to inhibit the growth of HeLa cells.

The Structural Resistanceâs Anatomy of Sweet Potato Leaves to Fungal Pathogen Sphaceloma batatas Samiyarsih, Siti; Juwarno, Juwarno; Muljowati, Juni Safitri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12116

Anatomical characters can be used as instructions to the structural resistance of plants to pathogen attack. Various pathogens attack sweet potato plants, such as the Sphaceloma batatas fungus that causes scurvy disease (scab). The aims of this research t test the structural resistance of sweet potato plants based on leaf anatomical character and intensity of disease attack due to the inoculated of S. batatas. The research was conducted from June to November 2016, using the Completely Randomized Design Experimental (RAL) method with a factorial pattern. The first factor was ten sweet potato cultivars and the second factor was the inoculum of S. batatas fungus each treatment with five replications. The character of leaf anatomy observed was thick of cuticle, thick of mesophyll, size and number of stomata and number of trichomes per 1 mm2 leaf area. Based on the research result, it was concluded that the inoculation of the fungus of S. batatas caused the decrease of stomata length and width on ten sweet potato cultivars. The highest intensity of disease attack was 14.33% and correlated with stomata length (r = 0.49). The anatomical structural resistance to scurvy can be used as a basis for determining crosses for obtaining superior sweet potato cultivars. The benefit of the research is to advise the community to cultivate sweet potatoes that have structural resistance to scurvy, such as cuticle and thick mesophyll, high trichomata density.

Selection of Soybean (Glycine max) Germplasm Against Biotrophic Fungi Disease Based on Anatomical Resistance Samiyarsih, Siti; Pratiwi, Ade Yuanita Putri; Muljowati, Juni Safitri; Fitrianto, Nur
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25109

The obstacle to increasing the soybean production is an infection of rust disease caused by the biotrophic fungus, Phakopsora pachyrhizi. The research objectives were to determine the anatomical resistance and the level of resistance of soybean cultivars against rust disease. The embedding method for observed leaf structural anatomy. The disease severity based on the method of International Working Group on the Soybean Rust (IWGSR) rating system.Â The experiment was arranged as a Completely Randomized Design (CRD) with Factorial Pattern and five times repetition. The first factor was soybean cultivars, namely Gepak Kuning, Slamet, Tanggamus, and Wilis. The second factor was P. pachyrhizi inoculation with 0 uredospores/mL (uninoculated) and 104 uredospores/mL (inoculated). The results showed that the soybean cultivars that have thicker cuticle and epidermis, high trichomes and low stomatal density, and low of stomatal conductance have better anatomical resistance to leaf rust disease. Wilis and Slamet cultivars are resistant cultivars, indicated by disease intensity of 20% and 24.6%, respectively. While the Tanggamus is moderately resistant cultivar and Gepak Kuning is a susceptible cultivar, indicated by disease intensity of 56.5% and 85.3%, respectively. The novelty of selection soybean germplasm against biotrophic fungal disease are important and effectiveness in order to increase the crop productivity. These three soybean cultivars potentially serve as genetic sources to develop high yielding soybean cultivars and resistant to rust disease.

Histopathological Evaluation of Soybean (Glycine max (L.) Merr.) Strains Resistance to Sclerotium rolfsii Disease Siti Samiyarsih; Chindy Ayu Erlina; Juni Safitri Muljowati; Nur Fitrianto
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 10, No 1 (2022)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i1.8907

Sclerotinia infection of stem and leaf of soybean Glycine max (L.) Merr. caused by the fungal pathogen of Sclerotium rolfsii has recently become more important in the Indonesian soybean production area. This study aimed to evaluate the level of resistance and intensity of infection by S. rolfsii in four soybean strains. The research was arranged in a factorial completely randomized design. The observed variables include the anatomy characteristics of leaves and stems of soybean and disease intensity caused by S. rolfsii. The data were analyzed quantitatively with the Analysis of Variance (ANOVA) at 95% and 99% confidence level, followed by the Least Significant Difference Test (Fisher’s LSD) at the level of 5%. Soybean leaves and stem anatomy inoculated by S. rolfsii showed a decrease in the stomatal density, epidermis thickness, and mesophyll thickness as well as a damaged cuticle, damaged stem epidermis, and swollen stem cortex. Four strains inoculated by S. rolfsii showed a higher disease intensity of 40%-80% compared to the resistant cultivar ('Dering') and susceptible cultivar ('Wilis'), showing disease intensity of 20% and 40%, respectively.

Pelatihan Pelatihan Budidaya Jamur Tiram Skala Rumah Tangga Untuk Mendukung Penyediaan Menu Harian Yang Bergizi Nuniek Ina Ratnaningtyas; Nuraeni Ekowati; Oedjiono; Juni Safitri Muljowati; Arif Rahman Hikam
JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Vol 2 No 3 (2021)
Publisher : Politeknik Piksi Ganesha Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37339/jurpikat.v2i3.599

Pelatihan budidaya Jamur Tiram Putih (Pleurotus ostreatus) di Rt.01//Rw06 dan Rt. 02/Rw01 Kelurahan Sumampir Purwokerto Utara ini, bertujuan memberikan keterampilan tambahan guna menambah penghasilan keluarga dan menunjang penyediaan menu harian bergizi. Menggunakan metode partisipatif dengan mengkombinasikan kegiatan ceramah, praktek, pembuatan demplot, dan pendampingan. Khalayak sasaran dilatih membuat rumah jamur, rak tempat baglog, memanen jamur, dan mengolah hasil budidaya menjadi jamur krispi, sop dan kerupuk. Baglog media tanam jamur yang digunakan berasal dari hasil produksi mitra pelaksana kegiatan. Jamur hasil budidaya memiliki kualitas yang baik, putih bersih, satu rumpun dapat mencapai berat 100g, ukuran tudung jamur antara 7-18cm. Hasil panen harian bervariasi antara 0,5-12kg, sehingga cara pemasaran yang diterapkan tergantung hasil panennya. Saat hasil panen berjumlah banyak, dijual di pasar tradisional, saat hasil panen sedikit, dikonsumsi sendiri atau dijual kepada tetangga sekitar rumah. Kegiatan pelatihan ini berdampak positif bagi warga di sekitar khalayak sasaran, karena dapat menunjang menu harian mereka.

Pengaruh Cercospora sp. terhadap Kandungan Asam Askorbat pada MekanismePatogenisitas Bercak Daun Tanaman Cabai : Kajian secara In vitro dan In planta Nasriyatun Yuliawati; Aris Mumpuni; Juni Safitri Muljowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Red chili is a vegetable commodity that has high economic value in Indonesia. Leaf spot disease caused by the fungus Cercospora sp. is one of the limiting factors in red chili production. The occurrence of leaf spot disease is determined by the success of the pathogenesis by the fungus Cercospora sp. In addition, red chilies that are resistant to leaf spot disease have higher ascorbic acid content than vulnerable red chilies. The purpose of this study was to determine the ability to grow pathogens Cercospora sp. on the medium which was given ascorbic acid and know the effect of inoculation of the pathogen Cercospora sp. against ascorbic acid content in red chili leaves (C. annuum L.). This study used an experimental design with a completely randomized design (CRD). In vitro tests carried out consisted of PDA and PDB medium which were given ascorbic acid with a concentration of 0 mg.l-1, 0,25 mg.l-1, 0,5 mg.l-1, 0,75 mg.l-1 and 1,0 mg.l-1. In planta testing was using hot chili red chili varieties, large red chili varieties and curly red chili varieties. The treatments that were tested included calculation of disease intensity and ascorbic acid content in red chili leaves. In vitro test the main parameters observed were the diameter colony of the fungus Cercospora sp. dan mycelium dry weight. In planta test the main parameters observed were the intensity of the disease, while the supporting parameters were the incubation period of the disease, the content of ascorbic acid in the red chili leaves, temperature and humidity. In vitro test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). In planta test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). The results showed that the pathogen Cercospora sp. able to grow well on the PDA medium and GDP medium which were given ascorbic acid. Inoculation of pathogen Cercospora sp. can increase ascorbic acid content in red chili leaves.

PENGARUH Colletotrichum coccodes TERHADAP KANDUNGAN ASAM ASKORBAT PADA MEKANISME PATOGENISITAS ANTRAKNOSA TANAMAN CABAI: KAJIAN SECARA IN VITRO DAN IN PLANTA fadhila meilasari; Juni Safitri Muljowati; Aris Mumpuni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Patogen Colletotrichum coccodes merupakan salah satu patogen yang dapat menginfeksi tanaman cabai dan menyebabkan penyakit antraknosa terutama pada bagian buah dan daun. Tanaman dengan kandungan asam askorbat tinggi memiliki ketahanan yang lebih tinggi terhadap serangan patogen. Tanaman cabai yang tahan memiliki kandungan asam askorbat yang lebih tinggi dibandingkan dengan tanaman cabai toleran maupun rentan. Tujuan penelitian ini adalah mengetahui kemampuan tumbuh patogen C. coccodes pada medium yang diberi asam askorbat dan mengetahui pengaruh inokulasi patogen C. coccodes terhadap kandungan asam askorbat pada daun cabai. Penelitian dilakukan di Laboratorium Mikologi dan Fitopatologi, Laboratorium Lingkungan, & Greenhouse Fakultas Biologi Universitas Jenderal Soedirman, Purwokerto. Penelitian ini menggunakan dua uji yaitu uji in vitro dan uji in planta dengan metode eksperimental Rancangan Acak Lengkap (RAL), Uji in vitro menggunakan A) Medium PDA diberi asam askorbat; B) Medium PDB diberi asam askorbat dengan perlakuan penambahan asam askorbat sebanyak 0 mg.L-1 (kontrol); 0,25 mg.L-1; 0,50 mg.L-1; 0,75 mg.L-1; dan 1 mg.L-1, diulang sebanyak lima kali. Variabel bebas yang digunakan yaitu berbagai dosis asam askorbat, variabel terikatnya adalah pertumbuhan patogen C. coccodes. Parameter utama yaitu diameter koloni dan bobot kering miselium. Uji in planta menggunakan tiga varietas cabai (V1: Cabai merah hot chili; V2 Cabai merah keriting; V3: Cabai merah besar), uji A) Uji intensitas penyakit pada tanaman dan B) Uji kandungan asam askorbat. Masing-masing kelompok uji diulang sebanyak lima kali. Variabel bebas yang digunakan adalah varietas cabai merah, variabel terikatnya adalah nilai kerusakan tanaman berdasarkan kategori yang diamati pada waktu pengamatan yang ditentukan. Parameter utama yaitu intensitas penyakit, dan parameter pendukung yaitu periode masa inkubasi, kandungan asam askorbat pada daun cabai, temperatur, kelembaban dan pH tanah. Data uji in vitro dan uji in planta yang diperoleh dianalisis menggunakan analisis ragam (ANOVA) dengan tingkat kepercayaan 95%, dan perlakuan yang memberikan perbedaan nyata dilanjutkan dengan Uji Beda Nyata Jujur (BNJ) pada tingkat kepercayaan 95%. Hasil penelitian pada uji in vitro menujukkan bahwa patogen C. coccodes memiliki kemampuan tumbuh yang baik pada medium PDA dan medium PDB dengan penambahan asam askorbat. Hasil penelitian pada uji in planta, inokulasi patogen C. coccodes pada daun cabai merah dapat meningkatkan kandungan asam askorbat pada tanaman cabai merah Kata kunci : Colletotrichum coccodes, Cabai Merah, Antraknosa, Asam askorbat.

Pemberian Inokulum Fungi Mikoriza Arbuskula (Fma) Campuran terhadap Kemunculan Penyakit Busuk Pangkal Batang Sclerotium pada Tanaman Cabai Rawit dan Cabai Merah Wira Dhyaksa Pradana; Uki Dwiputranto; Juni Safitri Muljowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Cayenne pepper (Capsicum frutescens) and red chili (Capsicum annuum), including vegetables and fruit are widely consumed by the public, and also have many benefits. At present, the market demand for cayenne pepper and red chili is very high, so equalization must be made from the production sector. The conventional way of handling such as the administration of pesticides or other chemicals is less effective because it causes side effects that have a large enough impact, so an alternative technique is used that is to use Arbuscular Mycorrhizal Fungi (FMA) thus, research on the administration of Arbuscular Mycorrhoid Fungi Inoculum is thus carried out. (FMA) Mixture of Sclerotium Stem Rot Rotation in Capsicum frutescens and Capsicum annuum. The purpose of this study was to determine the effect of mixed FMA inoculums in suppressing the intensity of sclerotium stem rot rot disease in cayenne pepper (Capsicum frutescens) and red chili peppers (Capsicum annuum) and to determine the optimal dose of mixed AMF in suppressing the intensity of sclerotium stem rot rot on cayenne plants (Capsicum frutescens) and red chili plants (Capsicum annuum). This study used a Completely Randomized Design (CRD) with different doses of mixed AMF inoculums (0, 10, 15, 20, 25 g FMA with zeolite / plant carrier medium). The results of this study indicate that the treatment of mixed AMF inoculums on the intensity of sclerotium stem rot disease in cayenne and red chili plants can reduce the intensity of Sclerotium stem rot disease by 22% and in red chili plants by 11%.

HUBUNGAN INTENSITAS PENYAKIT KARAT DENGAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max (L.) Merr.) PADA BEBERAPA VARIETAS BERBEDA Maman Maman; Juni Safitri Muljowati; Rochmatino Rochmatino
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The severity of plant disease is closely related to the development of the disease itself, including rusts caused by pathogenic fungi. This research aimed to determine the difference of rusts disease severity and to determine the correlation between rusts disease severity with the productivity of different varieties of soybean. The material used was inoculum of rusts pathogenic fungi, Phakopsora pachyrhizi Syd., and four varieties of soybean, i.e., Slamet, Lokon, Bromo, and Ringgit. This research was a split-plot design experimental with subplot was four different varieties of soybean, and the main plot was the control without the treatment of inoculation (In0). The spores suspension density of inoculum of P. pachyrhizi Syd applied was 104 spores/ml (In1). The main parameters observed in this study were the height of plants, the number of pods per plant, the number of seeds per plant, the wet and dry weight of the plant, and the total weight of 100 grains seed. The supporting parameters were the disease severity, the soil acidity, the humidity, and the air temperature. The results showed the plant with lowest rusts disease severity was Slamet variety (18,38%), and the highest was Ringgit variety (35,92%). The correlation test showed the increment of rusts disease severity reduced the number of pods and dry weight of the plant in Lokon variety.

EFEKTIVITAS PELET BIOFUNGISIDA Trichoderma harzianum MENGENDALIKAN Fusarium sp. PENYEBAB PENYAKIT REBAH SEMAI PADA BIBIT TANAMAN CAISIM (Brassica rapa var. parachinensis L) Rubiati Fadhilah; Juni Safitri Muljowati; Endang Sri Purwati
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

This research aimed to determine the effect of the interaction of the dosage with the application model of Trichoderma harzianum bio-fungicide pellet to the effectiveness of damping-off disease control caused by Fusarium sp. on the green mustard, and also to determine the most effective dosage and application model to control that disease. This research was experimental with the factorial completely randomized design. The factors were: the dosage of T. harzianum bio-fungicide pellet, i.e., 0 g (D0), 12,5 g (D1), 25 g (D2), 37,5 g (D3), 50 g (D4), 62,5 g (D5), and 75 g (D6); and the application model of pellet, i.e., T1, T2, T3 dan T4. This research analyzed the data using the test of variety difference with 5% and 1% degree of errors, followed by least significance difference test. The results showed the interaction between dosage and application model did not affect the effectiveness of the damping-off disease control on the green mustard seedling. The dosage of T. harzianum pellets and the application model of T. harzianum pellets independently gave a significant effect on the effectiveness of biological control agent of T. harzianum toward Fusarium sp. on the green mustard seedling. The dosage of 37,5 g per 50 green mustard individuals was the most efficient amount to control the damping-off disease. The 7-day continuous application and incubation of pellet to the seeding medium before pathogen inoculation and planting were better than any other application models.

Co-Authors Ade Winda Pradana Arif Rahman Hikam Aris Mumpuni Aris Mumpuni Chindy Ayu Erlina Eddy Tri Sucianto Endang Sri Purwati fadhila meilasari Fitrianto, Nur Iman Budisantoso Juwarno, Juwarno Maman Maman Nasriyatun Yuliawati Nuniek Ina Ratnaningtyas Nur Fitrianto Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Oedjiono Pratiwi, Ade Yuanita Putri Purnomowati Purnomowati Rochmatino Rochmatino Rubiati Fadhilah Siti Samiyarsih Siti Samiyarsih Siti Samiyarsih Uki Dwiputranto Uki Dwiputranto Wira Dhyaksa Pradana

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