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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Tropical and Infectious Disease
Prakoeswa, Cita Rosita
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Health Professions Medicine & Pharmacology Public Health

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Histoid Leprosy Rinasari, Umi; Sawitri, Sawitri; Listiawan, M. Yulianto; Prakoeswa, Cita Rosita; Agusni, Indropo; Santoso, Rachmat; Izumi, Shinzo
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 1 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.114 KB) | DOI: 10.20473/ijtid.v1i1.3719

Abstract

Histoid Leprosy is a variant of lepromatous leprosy with characteristic clinical and histopathological features. Usually it is occured in lepromatous patients who relaps after dapsone monotherapy, in those with dapsone resistance , sometimes even after multidrug treatment, or at times, de novo with characteristic clinical and histopathological features. A 36 years old male, originated from Papua, visited to the skin outpatient clinic with translucent shiny nodules on the left elbow and thumb for the last 18 months. The nodules were multiple, painless and firm. There were nasal congestion, tickening of ear lobes and loss of eye brows. Patient did not have any history of previous antileprotic treatment. Routine blood examination was normal. Bacteriological examination of slit skin smear revealed acid-fast bacilli of Bacterial Index 4+ and Morfologic Index 10%. Histopathology of skin suggested lepromatous leprosy of histoid type with characteristic interlacing bundles of spindle shaped cells. Anti-PGL1 antibody (ELISA) revealed high titer of IgM (>5.300 u/ml) and also IgG anti PGL-1 (>5.300 u/ml). Polymerase chain reaction examination test to detect M.leprae was positive and direct sequencing of M.leprae isolate shows no mutation, which means no resistancy to MDT treatment. Treatment with MDTWHO regiment give clinical improvements and the histoid lesions disappered after 3 months treatment.The histoid form of leprosy in this case developed without any prior treatment of anti leprotic drugs ( de novo ). Some theoretical aspects of the patho-mechanism of histoid leprosy are discussed.
Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
Co-Authors Aulia, Syifa DINAR ADRIATY INDROPO AGUSNI ISWAHYUDI ISWAHYUDI Linda Astari, Linda M. Yulianto Listiawan Rahmatari, Bandaru RATNA WAHYUNI Rinasari, Umi Santoso, Rachmat Sawitri Sawitri Sepling Paling SHINZO IZUMI Siti Kurniawati