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Response size of Digestive organs of Broiler that are fed containing Jack bean (Canavalia ensiformis) different levels of protein with protease enzyme supplementation Mahardhika, Brahmadhita Pratama; Ridla, Muhammad; Mutia, Rita; Naja, Miftakhun
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 31, No 2 (2021): August 2021
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2021.031.02.06

Abstract

This research was conducted to evaluate the use of Jack bean (Canavalia ensiformis), the level of protein diet, and the supplementation of protease enzymes to the size response of the starter phase of Broiler chicken digestive organs. Birds used in this study were 48 broilers of male chickens which were distributed into 3 observation factors and 3 replications (each replication consisted of 2 samples). The observation factor is Factor 1 are ingredients (basal diet based on soybean meal and a diet containing Jack bean), the second factor is the level of protein (22% and 19.5%) and the third factor is enzyme treatment (without enzymes and the addition of proteases). The design used was a completely randomized factorial design (RALF) and analysis of variance. The results showed that the decrease in protein level (from 22% to 19.5%) significantly high (P <.0.01) increased the length of the jejunum but could be reduced to produce the same length as basal feed (PK 22%) with the addition of the protease enzyme. The use of Jack bean significantly (P <0.05) increases the length of the jejunum but is reduced by the addition of the protease enzyme to the same as the basal diet produced by soybean meal. The addition of protease enzymes significantly reduced ileum weight and total small intestine weight (P <0.05) and significantlydecreased jejunum length (P <0.01). There was no interaction between ingredient, level of protein, and protease enzymes on size response of digestibility broiler organ.
Utilization of Jack Bean Seed (Canavalia ensiformis) with Treatments of Protease Enzyme Fed to Broiler in Starter Period Brahmadhita Pratama Mahardhika; Muhammad Ridla; Rita Mutia; Iyan Yuliana; Miftakhun Naja
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 31, No 3 (2021): December 2021
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2021.031.03.03

Abstract

The achievement of this study was to evaluate the use of jack bean seed and protease enzymes in different protein levels in the Broiler ration on performance and metabolizable energy. Two hundred and forty broilers were distributed into 3 object factors and 3 replications. The first factor was the difference of the ingredients (soybean meal-based basal ration and jack bean seed-contained rations), the second factor was the level of protein (22% and 19.5%) and the third factor was the enzyme treatment (without enzymes and with the inclusion of the proteases). A total of 2 birds from each treatment were assigned as a sample for metabolizable energy assay. The experimental design used was a factorial completely randomized design (FCRD). The data were analyzed using Analysis of Variance (ANOVA). The results showed that adding jack bean seed into the Broiler starter’s ration significantly (P <0.01) decreased the body weight gain (BWG) but it could be improved with the inclusion of protease enzyme. The metabolizable energy of the ration containing jack bean seed was significantly higher (P<0.01) than in the basal ration. The use of a low protein level ration (19.5%) significantly (P <0.01) showed the decrease in BWG, decrease in true metabolizable energy, and true corrected-nitrogen metabolizable energy but could be improved with the addition of protease enzyme. The use of the protease enzyme could improve the performance of broiler chickens fed with a low protein ration that contained Jack bean seed (Canavalia ensiformis).
The comparison of crude fat analysis between separated soxhlet method and one extractor soxhlet method for several feed ingredients Sofyan Sofyan; Eneh Maesaroh; Ratih Windyaningrum; Brahmadhita Pratama Mahardhika
Jurnal Teknologi dan Manajemen Pengelolaan Laboratorium Vol 3 No 2 (2020): Jurnal Teknologi Dan Manajemen Pengelolaan Laboratorium (Temapela)
Publisher : Labor Dasar dan Sentral Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/temapela.3.2.60-64.2020

Abstract

Determination of crude fat content (ether extract) using a separate Soxhlet method requires an extraction time of 4 to 6 hours to achieve 5-6 circulation for 1 sample of material. Given the fact that the analysis and extraction time are not time efficient, therefore an alternative method is needed with good accuracy and faster testing time. One alternative is the single-extractor Soxhlet method. The aims of this study were to evaluate the comparison of crude fat extraction results in the separated Soxhlet method and the Soxhlet method in one extractor for several feed ingredients. For the fat testing, this study used two methods, namely the separate method and the Soxhlet method of testing single extractor. The experiments were done by a completely randomized design with 2 treatments and 3 replications. The data was analyzed using the Student T-test. The results showed that the separate Soxhlet method and the single extractor Soxhlet method had no significant difference to the crude fat content of the concentrate feed ingredients with T> 0.05. The use of single extractor Soxhlet showed significant result with T< 0.01 which lower ​​than the separate Soxhlet method in forage samples on the crude fat concentration. Given the outcomes, the use of single extractor Soxhlet is more efficient than the separate Soxhlet method in terms of analysis time of a large number of samples.