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PENENTUAN NILAI SPF (Sun Protecting Factor) EKSTRAK ETANOL DAUN KALAKAI (Stenochlaena palustris (Burm F.) Bedd) Weni Puspita; Heny Puspasari; Athiah Masykuroh; Rika Fitria
Jurnal Komunitas Farmasi Nasional Vol. 3 No. 1 (2023): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

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Abstract

Tanaman Kalakai (Stenochlaena palustris (Burm F.) Bedd) adalah tanaman paku-pakuan yang tumbuh di daerah rawa gambut yang secara umum disebut lahan basah. Berdasarkan studi empiris daun dan batang digunakan oleh suku dayak untuk mengobati anemia, penambah ASI pada ibu menyusui, pereda demam, mengobati sakit kulit, dan juga sebagai pencuci perut. Daun kalakai muda memiliki kandungan metabolit sekunder yaitu flavanoid yang efektif sebagai antioksidan dan menangkal radikal bebas. Penelitian ini bertujuan mengetahui apakah ekstrak etanol daun kalakai muda (Stenochlaena palustris (Burm.F.) Bedd) memiliki aktivitas perlindungan tabir surya. Ekstrak etanol daun kalakai muda dibuat larutan induk dengan konsentrasi 1000 ppm, kemudian dilakukan pengenceran dengan variasi konsentrasi 50 ppm, 75 ppm, 100 ppm, 125 ppm, dan 150 ppm. Penentuan nilai SPF dilakukan dengan mengukur absorbansi dari ekstrak etanol daun kalakai muda menggunakan spektrofotometri UV-Vis dengan panjang gelombang antara ±290-320 nm. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kalakai muda dengan kategori proteksi ultra berpotensi sebagai tabir surya. Nilai SPF yang diperoleh pada tiap konsentrasi 50 ppm, 75 ppm,100 ppm, 125 ppm dan 150 ppm secara berturut-turut adalah 9,43 (proteksi maksimal); 9,47 (proteksi maksimal); 13,32 (proteksi maksimal); 18,25 (proteksi ultra) dan 21,22 (proteksi ultra).
ANTIBACTERIAL ACTIVITY OF SILVER NANOCOLLOID GEL BIOSINTHESIZED BY Alocasia macrorrhizos PLANT EXTRACT AGAINST Staphylococcus aureus : UJI AKTIVITAS ANTIBAKTERI GEL NANOKOLOID PERAK HASIL BIOSINTESIS MENGGUNAKAN EKSTRAK TANAMAN KELADI SARAWAK (Alocasia macrorrhizos (L.)) TERHADAP BAKTERI Staphylococcus aureus Athiah Masykuroh; Ika Ristia Rahman; Hairunnisa; Nine Okta Meliadlina; Putry Fajaria Hardiani
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

An antibacterial activity test has been conducted against Staphylococcus aureus bacteria on silver nanocolloids gel biosynthesized using Alocasia macrorrhizos plant extract which contains phenolic derivative compounds : polyphenols, flavonoids and cyanogenic glycosides. The AgNO3 concentration used in the silver nanocolloid biosynthesis process is 0.05 M;0,10 M and 0,15 M. The silver nanocolloids was diluted in the amount of 50% from the previous concentration and innovated into a gel form (formula I, II and III) then tested on Staphylococcus aureus bacteria. The measurement of of the clear zone formed around the disc paper was carried out to determine it’s antibacterial activity. Only the third variation meet the requirements of the physical evaluation test results as a gel form. All concentrations of silver nanocolloid gel preparations can inhibit the growth of Staphylococcus aureus bacteria as shown in observations obtained by the average diameter of the inhibition zone for formula I of 12.55 mm, formula II of 12.64 mm and formula III of 12.71 mm which is the zone of resistance formed was induced strong category (10-20 mm). The significance value with the One way ANOVA test is 0.000 (p<0.05), means that the average value of each variation is significantly different.