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All Journal JURNAL INFO KESEHATAN INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal teknologi Kesehatan Borneo Jurnal EduHealth JURNAL ILMIAH GLOBAL EDUCATION Innovative: Journal Of Social Science Research Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa
Ari Nuswantoro
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Number of Bacterial Colonies After Washing Hands with Antiseptic Soap and Regular Soap: A Study on Department of Health Analyst Students in Poltekkes Kemenkes Pontianak Ari Nuswantoro; Maulidiyah Salim; Slamet; Dinasti Aprillia
Jurnal teknologi Kesehatan Borneo Vol 1 No 2 (2020): Jurnal Teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v1i2.27

Abstract

Hand washing is a healthy activity carried out by many parties. Getting used to wash your hands properly will increase health. The soap on the market is plain hand washing soap and antiseptic handwashing soap. The second type is claimed to be more effective in reducing the amount of bacterial contamination than plain soap because it contains antimicrobial ingredients to increase product sales. This study aims to determine the differences in the number of bacterial colonies on the palms of students who wash their hands with antiseptic soap and plain soap among students of Analyst Department of Pontianak Health Polytechnic, Minister of Health. This research was quasi-experimental, with 24 respondents. Each respondent was asked to wash hands properly twice, the first with plain soap that does not contain an antiseptic (A) and the second with soap containing antiseptic (B). After examining the number of bacteria on the respondent’s thumb, the average number of bacterial colonies got in treatments A and B was 45,5 and 38,8 CFU, respectively. Mann-Whitney U statistical test results got a probability value of 0.391> 0.05, which means there is no difference in the number of bacterial colonies in the palms that washing hands with plain soap and with antiseptic soap. This study revealed the fact that the number of bacterial colonies in the group that washed hands with antiseptic soap was lower than those who washed their hands with ordinary soap.
Potential of Methanol Extract of Bungli Fruit Seed (Oroxylum Indicum) in Inhibiting the Growth of Escherichia coli Ari Nuswantoro; Natasya Intan Ramadhani
Jurnal teknologi Kesehatan Borneo Vol 2 No 1 (2021): Jurnal Teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v2i1.29

Abstract

Bungli (Oroxylum indicum) has been used for generations in Asia as traditional medicine. The seeds of the bungli are commonly used as traditional medicine to treat typhoid, cough, hypertension, internal fever, fever and diarrhea. The active compounds in this plant are known to have anti-inflammatory, antirheumatic, antifungal and antibacterial activities. Our research on methanol extract of bungli fruit seeds showed its potential to inhibit the growth of Escherichia coli bacteria that causes diarrhea. Bungli seed extract samples with a concentration of 25% showed an inhibition zone with a diameter of 9.22 mm, while at a concentration of 50% the diameter was 11.16 mm and 75% had a diameter of 12.88 mm. This size is indeed smaller than chloramphenicol (26 mm), but this potential can be greater if the extraction is carried out more intensively to extract the active compound, the sample concentration is increased, and the part or age of the plant with the highest content of the active compound is used; so that the Bungli plant is expected to be an alternative in overcoming Escherichia coli infection.
The Effectiveness of Chocolate in Reducing the Number of Methicillin-Resistant Staphylococcus aureus Colonies in Rattus norvegicus Edy Suwandi; Ari Nuswantoro; Sugito Sugito; Desi Wahyumarniasari; Muhammad Reza Setiawan; Dinasti Aprillia; Devi Nurfitri Bintang
JURNAL INFO KESEHATAN Vol 19 No 2 (2021): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (682.278 KB) | DOI: 10.31965/infokes.Vol19.Iss2.545

Abstract

Chocolate has long been understood to provide positive emotions and a good mood if consumed in moderation. Chocolate contains prebiotics naturally from its constituent ingredients produced during the production process. Prebiotics, frequently oligosaccharides, are substances which cannot be metabolized by the human digestive system but can be employed by a group of bacteria in the gut, understood as probiotics. The positive relationship among them provides benefits for the host in eliminating pathogens. One of the well-known pathogens which frequently cause infection either in the community or in hospitals is methicillin-resistant Staphylococcus aureus (MRSA). Since it was first identified in 1960, MRSA has caused health problems until today. Research conducted on two groups of Rattus norvegicus infected with MRSA and then fed chocolate revealed a decrease in the average number of bacterial colonies on the skin compared to the control group. In the group fed chocolate at a dose of 50 mg/day, the bacterial colonies decreased to 1.28 x 108 CFU/cm2 in 7 days, lower than in the control group (1.46 x 108 CFU/cm2) at the same time. While those fed 75 mg/day chocolate decreased to 2.70 x 107 CFU/cm2 and the three groups were significantly different (0.000<0.05). Prebiotics fermented by probiotics release short-chain fatty acids (SCFA), which compete with the pathogens for attaching to the epithelial wall so that pathogens lose space and nutrients to survive. However, the adverse effect of chocolate may occur because it contains sugar which is a nutrient for bacteria, but if the balance of normal flora and adequate intake of prebiotics are administered, the pathogen could be eliminated.
Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples Ari Nuswantoro; Jessica Ningtyas Berlianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2683

Abstract

Synthetic anticoagulants such as heparin, citric, and ethylenediaminetetraacetic acid (EDTA) are commonly used to prevent blood clots. In contrast, its widespread use in clinical laboratories is still constrained by price, its toxic nature, and its short shelf life. Therefore, an alternative material that is relatively cheap, non-toxic, and easy to obtain and process in a ready-to-use form is needed. Garlic contains allicin and ajoene, which are anti-platelet and anti-thrombogenic. This study’s aim is to explore the potential of garlic filtrate as an alternative anticoagulant.  Blood from 16 individuals was used and separated into four groups: non-anticoagulant, 50 μL/mL garlic filtrate, 100 μL/mL garlic filtrate, and 150 μL/mL heparin, for a total of 64 treatments. The Lee and White method showed that non-anticoagulated blood had normal clotting times (mean 8 minutes and 56 seconds), whereas heparin plasma and garlic filtrate plasma had longer clotting times (more than 20 minutes); and this is statistically different based on the ANOVA test with a significance value (p) of 0.000 < 0.05. On spectrophotometric measurements, the levels of calcium ions in heparinized plasma and serum were 8.66 mg/dL and 8.52 mg/dL, respectively, while in garlic plasma filtrate of 50 μL/mL and 100 μL/mL were 4.13 mg/dL and 3.58 mg/dL, respectively; this is also statistically different based on the ANOVA test with a significance value of 0.000 < 0.05. The differences indicate that garlic filtrate can extend clotting time and reduce calcium ions therefore it is worth reviewing as an alternative anticoagulant.
Combination of Aloe Vera and Chocolate to Reduce The Number of The Colony of Staphylococcus Aureus Edy Suwandi; Ari Nuswantoro; Sugito Sugito
Jurnal teknologi Kesehatan Borneo Vol 3 No 2 (2022): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v3i2.197

Abstract

Aloe vera (AV) and chocolate are good examples of functional foods because they contain prebiotics that can help the host defend itself against harmful bacterial infections such as Staphylococcus aureus. Many studies have used AV or chocolate alone to help fight infection, but not much is known about using both together. This study aims to count the number of colonies of S. aureus infected on mice’s skin (Mus musculus) after being given AV and cocoa in certain doses per kilogram of body weight. A total of 35 healthy male mice were infected with S. aureus in the superficial area of ​​the nape of the skin. After that, they were divided into seven groups, each consisting of 5 mice, and given the following treatments: given 50 mg/kg BW AV (group A), 50 mg/kg BW brown (B), 50 mg/kg BW combination of AV and chocolate (C), given 75 mg/kg BW AV (D), given 75 mg/kg BW brown (E), given 75 mg/kg BW combination of AV and chocolate (F), and control (without prebiotic administration), K). Mice that were given 75 mg/kg BW AV/chocolate/AV+chocolate had a better ability to reduce the number of bacteria than those that were given 50 mg/kg BW AV/chocolate/AV+chocolate. In addition, rats that were given AV and chocolate together had a better ability to reduce the number of bacteria than those that were given either only AV or chocolate alone. Prebiotics are both found in AV and chocolate but with different substances. This causes mice given a combination of prebiotics to be considered better than those given only one type of prebiotic source.
Pengaruh Penambahan Lidah Buaya (Aloe barbadensis Miller) pada Minyak Goreng Bekas terhadap Kadar Bilangan Asam Maulidiyah Salim; Gervacia Jenny Ratnawati; Ari Nuswantoro
Jurnal Vokasi Kesehatan Vol 7, No 1 (2021): JANUARI 2021
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.639 KB) | DOI: 10.30602/jvk.v7i1.833

Abstract

Minyak goreng bekas memiliki kadar bilangan asam yang tinggi dan dapat menyebabkan kerusakan minyak tersebut. Tujuan dari penelitian ini adalah untuk mengetahui apakah ada pengaruh penambahan lidah buaya (Aloe barbadensis Miller) pada minyak goreng bekas terhadap kadar bilangan asam. Desain penelitian yang digunakan adalah eksperimen semu, menggunakan Teknik purposive sampling. Sampel adalah minyak goreng yang telah mengalami proses penggorengan secara berulang yang dibagi menjadi ke dalam 4 kelompok perlakuan dan masing-masing 6 pengulangan sehingga total sampel menjadi 24. Kadar asam lemak bebas diperiksa dengan menggunakan metode alkalimetri. Nilai rata-rata kadar bilangan asam pada kelompok yang terdiri dari tanpa penambahan lidah buaya, dengan penambahan lidah buaya 25 gram, dengan penambahan lidah buaya 50 gram, dan dengan penambahan lidah buaya 75 gram berturut-turut adalah 0,17%, 0,15%, 0,11%, dan 0,09%.  Hasil uji regresi linier sederhana memberikan nilai probabilitas 0,000 (p < 0,05) sehingga dinyatakan ada pengaruh penambahan lidah buaya pada minyak goreng bekas terhadap kadar bilangan asam.
Lipid Profile of Prolanis Patients in Pontianak City Ari Nuswantoro; Dinasti Aprillia; Juliana Cristyaningsih
Jurnal teknologi Kesehatan Borneo Vol 4 No 1 (2023): Jurnal teknologi Kesehatan Borneo (In progress)
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v4i1.209

Abstract

Perubahan lingkungan, teknologi dan gaya hidup memicu naiknya kasus penyakit tidak menular, seperti diabetes melitus (DM) dan hipertensi (HT), yang bersifat kronis dan membawa risiko kematian terutama di negara berkembang seperti Indonesia. Dua penyakit tersebut dapat dideteksi dengan menilai profil lipid individu dimana kondisi dislipidemia dapat dianggap mengarah pada risiko terjadinya penyakit kardiovaskuler. Pemerintah Indonesia meluncurkan program Prolanis terhadap pasien DM dan HT yang salah satu kegiatannya adalah pemeriksaan profil lipid yang meliputi kadar kolesterol, trigliserida, HDL-kolesterol, dan LDL-kolesterol. Dari hasil pemeriksaan ini dapat dinilai rasio trigliserida terhadap HDL-kolesterol, yang merupakan prediktor paling kuat terhadap penyakit kardiovaskuler. Penelitian ini mengambil data pemeriksaan profil lipid dari 337 pasien Prolanis di Kota Pontianak. Hasil penelitian menunjukkan bahwa pasien DM didominasi oleh orang dengan hiperkolesterolemia (63,03%), trigliserida normal (56,36%), HDL-kolesterol normal (91,51%), LDL-kolesterol tinggi (83,03%), dan rasio trigliserida/HDL-kolesterol normal (60%). Demikian pula pada pasien HT, hiperkolesterolemia dan LDL-kolesterol tinggi dialami sebagian besar pasien (69,76 dan 84,30%), sedangkan trigliserida normal, HDL-kolesterol normal, dan rasio Trigliserida/HDL-kolesterol normal lebih mendominasi dengan persentase 61,61%, 94,19%, dan 68,03%. Hasil penelitian ini menunjukkan bahwa baik pasien DM maupun HT memiliki kecenderungan profil lipid yang normal dan menuju dislipidemia.
PROFILE OF RENAL FUNCTION LABORATORY EXAMINATIONS IN PROLANIS PATIENTS IN PONTIANAK CITY Ari Nuswantoro; Dinasti Aprillia; Indah Purwaningsih; Rizki Zanuba Arifah
Jurnal Laboratorium Khatulistiwa Vol 5, No 2 (2022): Mei 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i2.979

Abstract

Diabetes mellitus (DM) and hypertension is one of the chronic diseases that cause health problems in Indonesia. The government organizes a Management Program Chronic Disease (Prolanis) in order to manage patients with both of these diseases. One of the activities carried out is laboratory tests of urea and serum creatinine. This study aims to describe the number of DM and hypertension sufferers as well as serum urea and creatinine levels in Prolanis participants which checked themselves into the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak. The study was conducted using a total sampling method by looking at the medical record data of Prolanis participants, who will check themselves to the Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak, from January – December 2021 so that data were obtained from 333 patients consisting of 84 men and 349 women with an age range of 26 – 91 years. The results showed that 163 patients were diagnosed with DM and the remaining 170 people suffered from hypertension. The average value of urea levels to male patients is 36.4 mg/dL (14 – 113 mg/dL) and to women it is 27.3 mg/dL (11 – 80 mg/dL). The average value of creatinine levels to men is 1.3 mg/dL (0.5 – 5.3 mg/dL) and to women is 0.8 mg/dL (0.4 – 1.9 mg/dL). Urea and creatinine levels are theoretically associated with the incidence of DM and hypertension.
IDENTIFIKASI DAN PROFIL RESISTENSI BAKTERI PADA MINUMAN AIR TAHU DAN AIR TEBU DI WILAYAH KOTA PONTIANAK Aditia Aditia; Ari Nuswantoro; Herlinda Djohan; Bagus Muhammad Ihsan; Nurul Uq'ma; Anisya Nur Shafitri; Affifatuzahara Affifatuzahara; Fahma Ajeng Al-qorifah; Benedikta Anjelica
Jurnal Ilmiah Global Education Vol. 4 No. 4 (2023): JURNAL ILMIAH GLOBAL EDUCATION, Volume 4 Nomor 4, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/jige.v4i4.1860

Abstract

Tofu and sugarcane juice drinks are in great demand because they taste delicious and affordable. These drinks can be contaminated with pathogenic bacteria due to hygiene and poor environment. Contamination of pathogenic bacteria that are resistant to antibiotics is a serious health threat.  The objective of this study is to identify and provide antibiotic resistance bacteria from tofu and sugarcane juice drinks overview. This Study is a descriptive-observation with a cross-sectional design. Bacterial identification is carried out by culture, while resistance testing is carried out by Kirby-Bauer. Penicillin, amoxicillin, ampicillin, ciprofloxacin, chloramphenicol, gentamicin, tetracycline, and vancomycin were used. 43 samples of tofu water drink and 70 samples of sugarcane juice drink were selected by purposive sampling. Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, and Klebsiella pneumoniae founds in tofu drinks, while Enterobacter aerogenes, Salmonella typhi, Shigella dysenteriae, and Escherichia coli founds in sugarcane juice. Staphylococcus aureus resistant to penicillin (100%), Salmonella typhi resistant to penicillin (66.6%), Enterobacter aerogenes resistant to tetracycline (70%), Klebsiella pneumoniae resistant to penicillin (100%), Shigella dysenteriae resistant to ampicillin and gentamicin (100%), and Escherichia coli resistant to vancomycin (100%). Pathogenic-resistant bacteria in tofu and sugarcane juice drinks show the risk of spreading resistant bacteria which can trigger bacterial resistance with a wider spectrum.
Pengukuran Nilai MPN Pada Air Minum Isi Ulang Di Wilayah Kota Pontianak Yang Disterilisasi Dengan Portabel Ultraviolet Dan Portabel Filtrasi Nur Reza; Ari Nuswantoro; Ratih Indrawati; Bagus Muhammad Ihsan
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v3i6.7005

Abstract

Air merupakan kebutuhan utama manusia untuk bertahan hidup sedangkan sumber air bersih alami semakin banyak yang tercemar. Sebagai alternatif, masyarakat mendapatkan air minum dari depot air minum isi ulang (DAMIU) yang menggunakan metode sterilisasi portabel ultraviolet (UV) dan portabel filtrasi. Namun demikian, bakteri coliform masih ditemukan pada air minum karena air baku yang tercemar, alat yang digunakan tidak steril, dan minimnya wawasan masyarakat mengenai sanitasi dan higien DAMIU. Tujuan penelitian ini menghitung nilai most probable number (MPN) pada DAMIU di Kota Pontianak. Penelitian ini berbentuk deskriptif eksploratif berdesain cross-sectional. Data diperoleh dengan melakukan pemeriksaan MPN terhadap 36 sampel DAMIU. Hasil menunjukkan 14 (77,78%) DAMIU metode portabel ultraviolet dan 17 DAMIU (94,44%) portabel filtrasi menunjukkan nilai MPN di atas batas yang ditentukan. Banyaknya DAMIU yang tidak sesuai persyaratan kualitas air minum layak konsumsi sesuai Permenkes menunjukkan adanya risiko penularan penyakit yang disebabkan oleh coliform melalui air minum DAMIU di Kota Pontianak.
Co-Authors Aditia Aditia Affifatuzahara Affifatuzahara Anisya Nur Shafitri Azura azura Bagus Muhammad Ihsan Benedikta Anjelica Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprillia Dinasti Aprillia Dinasti Aprillia Edy Suwandi Edy Suwandi Fahma Ajeng Al-qorifah Gervacia Jenny Ratnawati Herlinda Djohan Indah Purwaningsih Jessica Ningtyas Berlianti Juliana Cristyaningsih Maulidiyah Salim Natasya Intan Ramadhani Nur Reza Nurul Uq'ma P Purwanto Ratih Indrawati Rizki Zanuba Arifah Slamet Sri Tumpuk Sugito Sugito Sugito Sugito