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All Journal Biomedical Journal of Indonesia Indian Journal of Forensic Medicine & Toxicology
Hardian
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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Effect of Artemisia vulgaris Extract on P53 Expression and Caspase-8 Expression (Study on Adenocarcinoma Mammae C3H Mice Given Adriamycin- Cyclophosphamide Chemotherapy Regimen) Sugiharto, Jonathan; Hardian; Selamat Budijitno
Biomedical Journal of Indonesia Vol. 7 No. 2 (2021): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i2.300

Abstract

Background: The incidence of breast cancer worldwide is still high. Surgery remains the top choice with other modalities of chemotherapy. radiation. and immunotherapy such as Artemisia vulgaris (AV). Purpose : The study was aimed to demonstrate that administration of AV extract increased the levels of p53 and Caspase-8 in adenocarcinoma mammae. Methods: This study used "Post test only control group design" on 24 females C3H mice that were randomly selected and divided into four groups : group K (control). P1 (chemotherapy). P2 (extract). and P3 (combination). Adenocarcinoma mammae comes from the inoculation of donor mice. Chemotherapy of Adriamycin 0.18 mg and Cyclophosphamide 1.8 mg were given in 2 cycles. AV 13 mg (0.2 ml) was given once daily orally. P53 and Caspase-8 levels were evaluated by imunohistochemical staining. Results: Mean of p53 and Caspase-8 levels were found in groups K. P1. P2. P3 were 22.06+1.73. 37.16+1.20. 24.60+1.08. 39.78+1.19 dan 17.16+1.28. 26.20+1.11. 24.60+1.08. 39.78+1.19. The statistical analysis showed that there were significant differences in the levels of p53 between groups of K vs P1. P3 (p=0.001). K vs P2 (p=0.048). P1 vs P2 (p=0.001). P1 vs P3 (p=0.039). P2 vs P3 (p=0.001). and in Caspase-8 between groups of K vs P1. P3 (p=0.001). K vs P2 (p=0.048). P1 vs P2 (p=0.001). P1 vs P3 (p=0.039). P2 vs P3 (p=0.001). Correlation analysis between p53 and Caspase-8 showed significant correlation (p=0.047 dan r=0.883). Conclusion: Artemisia vulgaris can improve the effectivity of Adriamycin- Cyclophosphamide chemotherapy on C3H mice with adenocarcinoma mammae in terms of elevated levels of P53 and Caspase-8.
Effect Phenytoin Therapy to Fibroblasts and Angiogenesis of Enterocutaneous Fistula in Wistar Rat Muhammad Budiman Irpan Bachtiar; Hermawan; Hardian; Ignatius Riwanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16901

Abstract

Background: Comprehensive wound care in enterocutaneous fistula (ECF) is the therapeutic of choicecurrently, which may increase of closure rate without surgery from 19 to 92%. Phenytoin has been reportedhave anticolagenase effect on wound healing is hoped to improve the ECF closure.Aim: The study was aimed to demonstrate the effect of phenytoin on closure of enterocutaneous fistula seenfrom the number of fibroblasts and angiogenesisMethods: This study was “Randomized Controlled trial with post test only group design” on 18 malewistar rats with ECF, that were divided randomly into three groups: group(K) control, P1(topical phenytoin),P2(oral phenytoin). After 7 days of treatment, they were terminated and histopathological examinations wereperformed to do fibroblast cell counting and the amount of angiogenesis by Hematoxilin Eosin staining.Results: Mean of the number of fibroblastin groups K, P1 and P2 were 69.50 ± 10.07, 155.50 ± 13.50 and182.16 ± 11.85 respectively (One way Anova P=0.001) and mean of the number of angiogenesis in groupsK, P1, and P2 were 95.66 ± 9.72, 178.66 ± 11.75, and 205,16 ± 9.74 respectively (One way Anova P=0.001).Post Hoc Test LSD showed thatthe number of fibroblast of group P1 vs K(P=0.001), P2 vs K(P=0.001),P1 vs P2(P=0.004) and the number of angiogenesis of group P1 vs K(P=0.001), P2 vs K(P=0.001), P2 vsP1(P=0.002).Conclusion: The therapy of topical and oral phenytoin increased the number of fibroblasts and angiogenesisin Wistar rat with ECF. Out come of oral phenytoin therapy better than topical.
Co-Authors Hermawan Ignatius Riwanto Muhammad Budiman Irpan Bachtiar Selamat Budijitno Sugiharto, Jonathan