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All Journal MEDIA MEDIKA INDONESIANA Jurnal Kedokteran Brawijaya Jurnal Kedokteran Diponegoro Biomedical Journal of Indonesia Care : Jurnal Ilmiah Ilmu Kesehatan Medica Hospitalia
Selamat Budijitno
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Effectiveness of Artemisia Vulgaris as Supplementation Against Chemotherapy of Mammae Adenocarcinoma to Reduce Expression of NF-κB and CD 34 (Study in C3H Mice Given Chemotherapy Adriamycin - Cyclophosphamide) Saputro, Bayu Teguh; Selamat Budijitno
Biomedical Journal of Indonesia Vol. 7 No. 2 (2021): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i2.292

Abstract

Intoduction: Breast cancer is a significant healthcare problem worldwide. Surgery remains the treatment of choice combined with other modalities such as chemotherapy, radiation, and immunotherapy such as Artemisia vulgaris (AV). Selective cytotoxicity of AV is intended as a supplementation to Adriamycin-Cyclophosphamide, improving the response rate of chemotherapy in adenocarcinoma mammae. Method: This study used a "Post-test only control group design" on 24 females C3H mice that were randomly selected and divided into four groups: group K (control), P1 (chemotherapy), P2 (extract), and P3 (combination). Adenocarcinoma mammae come from the inoculation of donor mice. Chemotherapy of Adriamycin 60 mg / m 2 and Cyclophosphamide 600 mg / m 2 were given in two cycles. AV 13 mg (0.2 ml) was given once daily orally. NF-κB expression and CD34were evaluated using imunohistochemical staining. Result: The expression of NF-κB and microvascular density of CD 34 were obtained in groups of K, P1, P2, P3 Statistical analysis showed significant decrease in the expression of NF-κB between groups K and P1, P2, P3. Correlation analysis between NF-κB expression with CD 34 was found to have significant correlation (p = 0,039 and r = 0,897). Conclusion:Artemisia vulgaris can reduce angiogenesis by decreasing NF-κB expression and the microvascular density CD34 of adenocarcinoma mammae of C3H mice treated with Adriamycin-Cyclophosphamide chemotherapy and can improve the effectivity.
Effect of Artemisia vulgaris Extract on P53 Expression and Caspase-8 Expression (Study on Adenocarcinoma Mammae C3H Mice Given Adriamycin- Cyclophosphamide Chemotherapy Regimen) Sugiharto, Jonathan; Hardian; Selamat Budijitno
Biomedical Journal of Indonesia Vol. 7 No. 2 (2021): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i2.300

Abstract

Background: The incidence of breast cancer worldwide is still high. Surgery remains the top choice with other modalities of chemotherapy. radiation. and immunotherapy such as Artemisia vulgaris (AV). Purpose : The study was aimed to demonstrate that administration of AV extract increased the levels of p53 and Caspase-8 in adenocarcinoma mammae. Methods: This study used "Post test only control group design" on 24 females C3H mice that were randomly selected and divided into four groups : group K (control). P1 (chemotherapy). P2 (extract). and P3 (combination). Adenocarcinoma mammae comes from the inoculation of donor mice. Chemotherapy of Adriamycin 0.18 mg and Cyclophosphamide 1.8 mg were given in 2 cycles. AV 13 mg (0.2 ml) was given once daily orally. P53 and Caspase-8 levels were evaluated by imunohistochemical staining. Results: Mean of p53 and Caspase-8 levels were found in groups K. P1. P2. P3 were 22.06+1.73. 37.16+1.20. 24.60+1.08. 39.78+1.19 dan 17.16+1.28. 26.20+1.11. 24.60+1.08. 39.78+1.19. The statistical analysis showed that there were significant differences in the levels of p53 between groups of K vs P1. P3 (p=0.001). K vs P2 (p=0.048). P1 vs P2 (p=0.001). P1 vs P3 (p=0.039). P2 vs P3 (p=0.001). and in Caspase-8 between groups of K vs P1. P3 (p=0.001). K vs P2 (p=0.048). P1 vs P2 (p=0.001). P1 vs P3 (p=0.039). P2 vs P3 (p=0.001). Correlation analysis between p53 and Caspase-8 showed significant correlation (p=0.047 dan r=0.883). Conclusion: Artemisia vulgaris can improve the effectivity of Adriamycin- Cyclophosphamide chemotherapy on C3H mice with adenocarcinoma mammae in terms of elevated levels of P53 and Caspase-8.
Effect of Artemisia vulgaris Extract for Increasing IL-12 and Granzyme Expression (Study on Mammary Adenocarcinoma C3H Mice Given Adriamycin- Cyclophosphamide Chemotherapy Regimen) Hardanto, Gery Rifano; Selamat Budijitno
Biomedical Journal of Indonesia Vol. 7 No. 3 (2021): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i3.539

Abstract

Background: The incidence of breast cancer worldwide is still high. Surgery remains the top choice with other modalities of chemotherapy, radiation, and immunotherapy such as Artemisia vulgaris (AV). The study was aimed to demonstrate that administration of AV extract increased p53 expression and the apoptotic index in mammary adenocarcinoma. Methods: This study used a "Post-test only control group design" on 24 females C3H mice that were randomly selected and divided into four groups: group K (control), P1 (chemotherapy), P2 (extract), and P3 (combination). Mammary adenocarcinoma comes from the inoculation of donor mice. Chemotherapy of Adriamycin 0,18 mg and Cyclophosphamide 1,8 mg were given in 2 cycles. AV 13 mg (0.2 ml) was given once daily orally. IL-12 and granzyme expression were evaluated by immunohistochemical staining. Results : Mean of IL-12 and Granzyme expression were found in groups K, P1, P2, P3 were 50,40 ± 1,56, 60,28 ± 1,54, 53,48 ± 1,35, 75,40 ± 1,46 dan 14,96 ± 0,61, 24,86 ± 1,21, 17,14 ± 1,02, 26,62 ± 0,70. The statistical analysis showed that there were significant differences in the IL-12 between groups of K vs P1 (0,001), P1 vs P2 (0,001), K vs P2 (0,028), P1 vs P3 (0,001), K vs P3 (0,001), P2 vs P3 (0,001) and in granzyme expression between groups of K vs P1 (0,001), P1 vs P2 (0,001), K vs P2 (0,010), P1 vs P3 (0,047), K vs P3 (0,001), P2 vs P3 (0,001). Correlation analysis between IL-12 and Granzyme expression were found significant correlation (p = 0,001 and r = -0,911). Conclusion: Artemisia vulgaris can improve the effectivity of Adriamycin-Cyclophosphamide chemotherapy on C3H mice with mammary adenocarcinoma in terms of elevated IL-12 and Granzyme expression.
Hubungan Antara Tingkat Depresi pada Penderita Kanker Payudara Lanjut Lokal dengan Kadar Kortisol Serum Darah Selamat Budijitno
Medica Hospitalia : Journal of Clinical Medicine Vol. 3 No. 2 (2015): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.897 KB) | DOI: 10.36408/mhjcm.v3i2.220

Abstract

Latar belakang : Stress neurologis, melalui sitokin-sitokin internal di dalam sawar otak akan menyebabkan sekresi kortisol oleh kelenjar adrenal melalui HPA axis. Tujuan penelitian ini adalah membuktikan bahwa terdapat korelasi antara pengaruh depresi terhadap kadar kortisol pada penderita tumor payudara stadium III B. Metode : Penelitian ini merupakan penelitian klinis CrossSectional, yang dilakukan pada 40 pasien wanita penderita kanker payudara duktal invasif stadium lanjut lokal (stadium IIIA, IIIB, dan IIIC). Tingkat depresi diukur dengan quesioner standar Beck Depression Inventory (BDI). Kadar kortisol dihitung dengan metode ELISA, dan diambil dari serum sampel pada jam 09.0010.00. Hasil : Kadar kortisol rerata 254,98; SD 48,65 ng/ml, hasil BDI minimum 4, maksimal 9, dengan median 7. Berdasarkan hasil Spearman corellation terdapat hubungan bermakna antara nilai BDI dengan kadar kortisol (p<0,001, r=0,868). Simpulan : Sesuai dengan teori yang ada, kadar kortisol berhubungan erat dengan ekspresi kortisol. Proses sitokin-sitokin internal di otak pada pasien dengan IDC masih berlangsung sesuai teori, tetapi hal ini masih perlu diteliti lebih lanjut lagi. Pada penelitian ini juga terbukti quesioner BDI mempunyai korelasi yang kuat untuk pengukuran tingkat depresi yang dilihat dari parameter kortisol.
Perbandingan Kadar Estrogen Serum dan TGF β-1 Plasma pada Penderita BPH-Non BPH di Atas 50 Tahun dan Usia Muda Eriawan Agung Nugroho; Selamat Budijitno
MEDIA MEDIKA INDONESIANA 2013:MMI VOLUME 47 ISSUE 1 YEAR 2013
Publisher : MEDIA MEDIKA INDONESIANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.887 KB)

Abstract

ABSTRACT Comparison of estrogen serum and TGF β-1 plasma levels in benign prostate hyperplasia (BPH) and non BPH patients, among 50-years old and young patients.Background: Benign prostatic hyperplasia (BPH) is often found in older men. This disease affects an estimated 70% of men over 60 years, increased by 90% in men over 80 years age. Many scientists tried explores the hypothesis of the mechanism of occurrence of BPH. The purpose of study compared the levels of estrogen and transforming growth factor (TGF) ß-1 in plasma related to the development of BPH, in BPH patients and non-BPH over 50 years and young age.Methods: A clinical analysis study was carried out on subjects who were divided into 3 groups: patients with BPH >50 years, non-BPH >50 years and younger non-BPH aged 30-40 years. T-test was used to analyze the data. Serum estrogen and plasma TGF β-1 were assessed using ELISA method.Results: There was no significant difference (p=0.129) between the level of estrogen in BPH patients >50 years (140.091±43.649) and non BPH patients >50 years (63.69±18.757) but there was a significant difference (p=0.015) between level of plasma TGF ß-1 BPH patients >50 years (10.47±4.507) and non BPH patients >50 years of age (25.8±16.1103). No significant differences (p=0.348) were found between serum estrogen level of non-BPH >50 years (63.69±18.757) and the young age (57.17±10.2748), and between plasma TGF ß-1 non-BPH group >50 years (25.80±16.1103) and the younger age group (31.4±17,576)(p=0.496).Conclusion: There is difference in the level of TGF ß-1 between younger and older age groups. Elderly men are recommended for early prostate examination, because at the age of 50 years there is already a tendency of enlarged prostate gland. Levels of transforming growth factor β-1 can predict prostate enlargement.Keywords: Estrogen, TGF ß-1, benign prostate hyperplasia (BPH)ABSTRAKLatar belakang: Benign prostate hyperplasia (BPH) sering ditemukan pada pria usia lanjut. Penyakit ini terjadi pada 70% pria di atas 60 tahun, dan meningkat hingga 90% pada pria di atas 80 tahun. Banyak ilmuwan mencoba mengetengahkan hipotesis faktor yang berpengaruh terjadinya BPH. Tujuan penelitian ini membandingkan tingkat estrogen dan transforming growth factor (TGF) ß-1 dalam plasma terkait pembentukan BPH.Metode: Penelitian klinik analitik dilakukan pada subyek yang dibagi 3 kelompok, pasien dengan BPH >50 tahun, pasien non-BPH berusia >50 tahun dan muda non-BPH berusia 30-40 tahun. Analisis data dengan uji beda kadar estrogen serum dan TGF β-1 plasma ketiga kelompok. Kadar TGF ß-1 dan estrogen diukur dengan metoda ELISA.Hasil: Serum estrogen pasien BPH usia >50 tahun (140,091±43,649) lebih tinggi tetapi tidak berbeda bermakna (p=0,129) dibandingkan non BPH usia >50 tahun (63,69±18,757). Estrogen serum non-BPH usia >50 tahun (63,69±18,757) dibandingkan usia muda (57,17±10,2748) tidak berbeda bermakna (p=0,348). TGF ß-1 plasma pasien BPH usia >50 tahun (10,47±4,507) lebih rendah (p=0,015) dibandingkan pasien non BPH usia >50 tahun (25,8±16,1103). TGF ß-1 plasma kelompok non-BPH >50 tahun (25,80±16,1103) dibandingkan kelompok usia muda (31.04±17,576) tidak berbeda bermakna (p=0,496).Simpulan: Ada perbedaan antara tingkat TGF β-1 pada kelompok yang lebih tua. Laki-laki usia tua disarankan lebih dini melakukan pemeriksaan prostat, usia >50 tahun cenderung didapatkan pembesaran kelenjar prostat. Pemeriksaan kadar TGF -1 dapat memprediksi risiko pembesaran kelenjar prostat.
EFEKTIFITAS ALOE VERA (ALOE BARBADENSIS MILLEER) DALAM PENANGANAN HAND FOOT SYNDROME PADA PASIEN KANKER PAYUDARA YANG MENDAPAT KEMOTERAPI CAPECITABINE PER ORAL Paramita Nindya Hapsari; Yan Wisnu Prajoko; Selamat Budijitno
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 7, No 2 (2018): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.053 KB) | DOI: 10.14710/dmj.v7i2.20674

Abstract

Latar Belakang: Kemoterapi merupakan salah satu terapi yang sudah banyak dipakai untuk pengobatan kanker yang terbukti dapat meningkatkan survival rate pasien. Namun, kemoterapi dapat memberikan efek negatif bagi penggunanya. Salah satunya ialah Hand foot syndrome. Hand foot syndrome adalah efek samping kemoterapi yang sering ditemukan dan perlu penanganan yang tepat. Selain urea cream, aloe vera juga dapat melindungi kulit karena bekerja sebagai pelembab dan anti inflamasi.Tujuan: Membuktikan pengaruh aloe vera dalam penanganan hand foot syndrome pada pasien kanker payudara yang mendapat kemoterapi capecitabine per oral.Metode: Penelitian ini merupakan penelitian eksperimental dengantwo groupspretest-posttest design. Penelitian dilakukan di RSUP Dr. Kariadi, Semarang. Sampel penelitiandibagi menjadi dua kelompok, yaitu kelompok perlakuan dan kelompok kontrol (n=33) yang diberikan pretest, lalu pemberian gel aloe vera, kemudiandiberikanposttest. Skor hand foot syndrome setelah diberikan gel aloe vera dianalisis dengan uji Kendall’s tau b dan Mann Whitney, skor kualitas hidup di analisis dengan Paired T test, dan uji korelasi antara grade hand foot syndrome dengan kualitas hidup pasien kanker payudara menggunakan uji Spearman. Hasil: Baik pada kelompok kontrol maupun kelompok perlakuan, masing-masing mengalami perbaikan skor hand foot syndrome dengan hasil pada kelompok kontrol (p=0,025) dan kelompok perlakuan (p=0,008)Kesimpulan: Terdapat perbaikan skor hand foot syndrome pada pasien kanker payudara yang mendapat kemoterapi capecitabine per oral yang diberikan gel aloe vera.
RIWAYAT USIA PERTAMA MENARCHE ≤12 TAHUN BERHUBUNGAN DENGAN KEJADIAN KANKER PAYUDARA PADA WANITA USIA SUBUR Siwi Ariana; Selamat Budijitno; Suhartono Suhartono
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 8, No 2 (2020)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.914 KB) | DOI: 10.33366/jc.v8i2.1230

Abstract

Breast cancer is a continuous cell growth that spreads without control in breast tissue. Young women especially those who are in reproductive ages with breast cancer tend to have more malignant cancer and worse prognosis. The purpose of this study is to prove history of first age menarche ≤12 years associated with the incidence of breast cancer in reproductive ages. Research design analytic observational study used a control case study design. The population in this study were reproductive women aged 15-49 years who were examined at the Oncology clinic in Tugurejo General Hospital with anatomical pathology results. The samples in this study were 42 cases and 42 controls with consecutive sampling technique. The data were analyzed univaraiate anda bivariate by chi-square. Results research history of first age menarche ≤12 years releted of breast cancer with value p= 0,005; OR= 4,015; 95% CI= 1,618-9,966. Conclusion history of the first age of menarche ≤12 years is related to the incidence of breast cancer in women of childbearing age. The researchers can add new variables about risk factors for breast cancer.
Effect of Artemisia vulgaris Extract on Granzyme Expression and Tumor Mass Diameter (Study of Adriamycin Cyclophosphamide Chemotherapy in Adenocarcinoma Mammae C3H Mice Model) Gery Rifano Hardanto; Selamat Budijitno; Hardian Hardian
Jurnal Kedokteran Brawijaya Vol. 31 No. 4 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.04.1

Abstract

Breast cancer incident continues to increase globally. The surgical management can be combined with other therapeutic modalities, including chemotherapy, radiation, and immunotherapy, such as Artemisia vulgaris (AV). This study aimed to determine the effect of AV extract on Granzyme expression and tumor mass diameter growth of C3H mice with adenocarcinoma mammae. Twenty-four female C3H mice were randomly divided into groups of K (control), P1 (AC chemotherapy), P2 (AV extract), and P3 (combination). Adenocarcinoma mammae were inoculated from donor mice. Two cycles of chemotherapy by Adriamycin 0.18 mg and Cyclophosphamide 1.8 mg were given intravenously, while Artemisia vulgaris 13 mg (0.2 ml) was given orally once per day. Granzyme expression was assessed using immunohistochemical staining, while tumor mass diameter growth was measured using tumor calipers. There was a significant negative correlation between and tumor mass diameter growth (p=0,001 and r=-0,911). Artemisia vulgaris increases the apoptotic effects of Adriamycin-Cyclophosphamide chemotherapy by increasing Granzyme expression and decreasing tumor mass diameter growth in adenocarcinoma mammae C3H mice.
Co-Authors Eriawan Agung Nugroho Gery Rifano Hardanto Gery Rifano Hardanto Hardian Hardian Hardian Paramita Nindya Hapsari Saputro, Bayu Teguh Siwi Ariana Sugiharto, Jonathan Suhartono Suhartono Yan Wisnu Prajoko