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Paulus, James JH
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Earth & Planetary Sciences

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Uji Potensi Ekstrak Kasar Teripang Laut Holothuria atra Untuk Anti Kanker Dengan Menggunakan Metode Brine Shirmp Lethality Test Baramuli, Yunita; Kawung, Nickson; Paulus, James JH; Rumengan, Inneke FM; Wagey, Billy Th; Manginsela, Fransin
JURNAL PESISIR DAN LAUT TROPIS Vol 9, No 3 (2021): JURNAL PESISR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36520

Abstract

The aim of this research is to test the potency of Sea cucumber crude extract Holothuria atra using the shrimp larvae Artemia salina Leach. The sample were take from waters of tateli at Minahasa District. Sample preparation and the potential test were done at biology moleculer and marien pharmacy laboratorium of Faculty of Fhiseries and Marine Science Sam Ratulangi University. The sample macerated once with ethanol for 24 hours. Extract of the sample were conentrated using rotary evaporator. Using the  Brine Shrimp Lethality Test (BSLT) method  with shrimp larvae Artemia salina, Leach. As the sample dry weight  of the sample is 420 g after macerated with ethanol then decreased to 18 g after evaporated. Probit analysis of larva mortalityt data as the indicator of anti-cancer potential shown the value LC5030.01 mg/l. Bioactive compounds of sea cucumber H. arta has potency to be develop as anti-cancer material. Keywords: Anti cancer, Sea cucumber, Holothuria atra, Artemia salina leach   
BAKTERI RESISTEN MERKURI PADA ALGA COKLAT Padina australis DARI PERAIRAN KIMA BAJO SULAWESI UTARA Van Gobel, Sri Winisari; Mantiri, Desy MH; Paulus, James JH; Rompas, Rizald Max; Rumampuk, Natalie D; Londong, Sammy
JURNAL PESISIR DAN LAUT TROPIS Vol 9, No 3 (2021): JURNAL PESISR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36522

Abstract

Alga laut jenis Padina australis merupakan spesies alga coklat divisi Phaeophyta (alga cokelat) yang umumnya tersebar di perairan laut. Spesies ini mampu bertahan hidup di perairan yang mempunyai kualitas air rendah. Tujuan penelitian ini adalah untuk mengisolasi bakteri resisten merkuri pada alga P. australis, mengetahui bentuk sel dan karakteristik bakteri. Metode yang digunakan adalah isolasi bakteri secara konvensional dan diuji pada senyawa merkuri klorida (HgCl2.) Hasil uji morfologi bakteri dari alga P. australis adalah Gram positif berbentuk monobasil dan streptobasil serta mempunyai kemampuan resisten terhadap merkuri pada konsentrasi 250, 500 dan 1000 ppm. Uji antibiotik dari bakteri resisten merkuri tersebut dilakukan terhadap antibiotik amoxicillin, cefixime dan doxycycline. Hasil menunjukan bahwa antibiotik amoxicillin mempunyai zona hambat 0,4 mm, antibiotik cefixime dengan zona hambat 0,1 mm. dan doxycycline mempunyai zona hambat tertinggi yaitu 1,8 mm, terlihat bahwa bakteri dapat mengurangi efektifitas suatu obat. Kata Kunci : Alga coklat, bakteri Gram positif, bakteri resisten merkuri, antibiotik
KANDUNGAN KARBON (C) SERASAH MANGROVE DI DESA PONTO KECAMATAN WORI KABUPATEN MINAHASA UTARA Razak, Abd; Sondak, Calvyn FA; Paulus, James JH; Mamangkey, Noldy GF; Rimper, Joice RTSL; Sangari, Joudi RR
JURNAL PESISIR DAN LAUT TROPIS Vol 10, No 1 (2022): JURNAL PESISR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.10.1.2022.39109

Abstract

Mangrove forests have a role as an absorber of carbon dioxide (CO2) from air and sea therefore they play an important role in mitigating climate change. The problems that exist today are the decline in the area of mangrove forests due to the conversion of land functions into ponds, and development in coastal areas. Decrease of mangrove forest areas can lead to reduced its ability to absorb and storage carbon dioxide (CO2). The purposes of this study were to analyze litter carbon content of mangrove leaf litter in the mangrove forest of Pontoh Village, Wori District, North Minahasa Regency and estimate the carbon content (C) in mangrove litter per hectare per year. Based on the results of the analysis, the total average percentage of carbon content in mangrove litter in Ponto Village, Wori District, North Minahasa Regency was 29.71% C/day, and from the estimation results, the average carbon content (C) in mangrove litter is 3.68 tons/ha/year.  Key Word: mangrove, litter, carbon content
Co-Authors Baramuli, Yunita Billy Theodorus Wagey Inneke F. M Rumengan Kawung, Nickson Londong, Sammy Mamangkey, Noldy G.F Manginsela, Fransin Mantiri, Desy M. H Natalie D Rumampuk Razak, Abd Rimper, Joice RTSL Rizald Max Rompas, Rizald Max Sangari, Joudi RR Sondak, Calvyn FA Van Gobel, Sri Winisari