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All Journal JURNAL PESISIR DAN LAUT TROPIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Kawung, Nickson
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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PENEMPELAN LARVA SIPUT LAUT (HALIOTIS VARIA) : SUATU PERCOBAAN DI LABORATORIUM Ompi, Medy; Kawung, Nickson; Calvyn, Calvyn; Sondak, F. A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.816 KB) | DOI: 10.24002/biota.v15i3.2597

Abstract

Settlement of abalone (Haliotis varia) focusing on substrate quality and larval behavior were studied. Four types of substrate, shell and mucous, algae Gracilaria sp and Halimeda sp, and plastic petri-dish were used to test whether the larva might respond and settle on those substrates. At the same time, the mortality of larvae was also determined during the settlement. Without petri-dish, substrate of shell and mucous, algae, as well as polyvinyl chloride (PVC) plate were tested to find out substrate preferences when settlements occured. The result showed that variation of substrata, shell and mucous, algae and plastic ?petri-dish? possessed a stimuli responded by larva to settle. Shell and mucous as well as substrate algae were attractive among of substrata, since high of settlement on it were recorded. High larval mortality on shell and mucous as well as on substrata of algae were recorded. Substrate of shell and mucous and alga were favorable to substrata when settling. Several factors affecting settlement of larval abalone were discussed.
Uji Potensi Ekstrak Kasar Teripang Laut Holothuria atra Untuk Anti Kanker Dengan Menggunakan Metode Brine Shirmp Lethality Test Baramuli, Yunita; Kawung, Nickson; Paulus, James JH; Rumengan, Inneke FM; Wagey, Billy Th; Manginsela, Fransin
JURNAL PESISIR DAN LAUT TROPIS Vol 9, No 3 (2021): JURNAL PESISR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36520

Abstract

The aim of this research is to test the potency of Sea cucumber crude extract Holothuria atra using the shrimp larvae Artemia salina Leach. The sample were take from waters of tateli at Minahasa District. Sample preparation and the potential test were done at biology moleculer and marien pharmacy laboratorium of Faculty of Fhiseries and Marine Science Sam Ratulangi University. The sample macerated once with ethanol for 24 hours. Extract of the sample were conentrated using rotary evaporator. Using the  Brine Shrimp Lethality Test (BSLT) method  with shrimp larvae Artemia salina, Leach. As the sample dry weight  of the sample is 420 g after macerated with ethanol then decreased to 18 g after evaporated. Probit analysis of larva mortalityt data as the indicator of anti-cancer potential shown the value LC5030.01 mg/l. Bioactive compounds of sea cucumber H. arta has potency to be develop as anti-cancer material. Keywords: Anti cancer, Sea cucumber, Holothuria atra, Artemia salina leach   
Co-Authors Baramuli, Yunita Billy Theodorus Wagey Calvyn, Calvyn Inneke F. M Rumengan Manginsela, Fransin Medy Ompi Paulus, James JH Sondak, F. A.