<![CDATA[Abstrak __________________________________________________________________________________________ Telah dilakukan penelitian tentang isolasi, identifikasi, dan elusidasi struktur senyawa alkaloid dalam ekstrak metanol-asam nitrat dari biji mahoni bebas minyak (Swietenia macrophylla King). Biji mahoni diambil minyaknya dengan cara ekstraksi soxhlet menggunakan pelarut petroleum eter selama 8 jam. Isolasi senyawa alkaloid menggunakan metanol dan larutan asam nitrat 10%. Sebanyak satu gram ampas biji mahoni bebas minyak dicampur dengan 1 mL larutan asam nitrat 10%, kemudian ditambah 5 mL metanol dalam erlenmeyer bertutup disertai pengocokan selama 5 menit pada suhu 60oC menggunakan penangas air, setelah itu disaring, ditambah 1 mL larutan amonia 10%, kemudian disaring, dan dipekatkan. Ekstrak kemudian dianalisis dengan KLT, kemudian dipisahkan dengan kromatografi kolom. Sebagai penyerap pada kolom digunakan silika gel 40, panjang kolom 27 cm, diameter 2,8 cm, eluen yang digunakan adalah kloroform:metanol (95:5), dan jumlah tetesan 15-20 per menit. Setiap fraksi yang diperoleh dari kromatografi kolom dianalisis dengan KLT menggunakan lampu UV. Uji alkaloid menggunakan pereaksi Dragendorff 130 dan 132. Fraksi yang positif merupakan fraksi yang mengandung alkaloid. Kemudian fraksi ini dianalisis lebih lanjut untuk identifikasi struktur dengan GC, spektrometer IR, UV, dan 1HNMR. Hasil analisis menunjukkan bahwa dalam ekstrak diperkirakan terdapat senyawa alkaloid yakni 3,4,5-trietil-6-metoksi-2-metil-1,2-dihidro-piridin.  Abstract __________________________________________________________________________________________ It has been conducted a research on the isolation, identification and structural elucidation of alkaloid compounds in methanol-nitric acid extract of the oil-free mahogany seeds (Swietenia macrophylla, King). The oil was taken from mahogany seed by Soxhlet extraction and using petroleum ether solvent for 8 hours, while the isolation of alkaloid compound used methanol and 10 % nitric acid solution. A gram of oil-free mahogany slag was mixed with 1 mL of 10 % nitric acid solution, then it was added with 5 mL of methanol in a cap erlenmeyer and stirred for 5 min at 60 °C by using water bath, after that it was filtered and added by 1 mL of 10 % ammonia solution, then it was filtered and concentrated. The extract was analyzed by TLC and separated by column chromatography. As absorbent, it used silica gel column 40 with length of 27 cm, diameter 2.8 cm, while the eluent that was used was chloroform : methanol (95:5), and the number of droplets was 15-20 per minute. Each fraction obtained from column chromatography was analyzed by TLC using UV light. While, alkaloids test was analyzed by using Dragendorff reagent 130 and 132. Positive fraction is the fraction containing alkaloids, then this fraction was analyzed further more to identify the structure by using GC, IR spectrometer, UV and 1HNMR. The analysis showed that the extract was estimated that there are alkaloid compounds consisting of 3,4,5-triethyl-6-methoxy-2-methyl-1,2-dihydro-pyridine.]]>
