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All Journal Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research)
Wa Ode Santa Monica
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Agriculture, Biological Sciences & Forestry Veterinary

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The The identification of gram-negative bacteria and their effects on liver histopathology of Amazon Sailfin Catfish (Pterygoplichthys pardalis) in Lapompakka and Sidenreng Lakes, Wajo. Hairul Afzan; Andi Magfirah Satya Apada; Andi Rianti Rhasinta; Wa Ode Santa Monica
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 5 N0. 2, JULY 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v5i2.19105

Abstract

Amazon Sailfin Catfish (Pterygoplicththys pardalis) or commonly known as Plecos is originated from Amazon River in South America Continent. The aims of this study are to identify and isolate pathogenic gram-negative bacteria in the liver of the plecos from Lapompakka and Sidenreng Lakes and to observe the histopathological changes in liver identified with gram-negative bacteria infection. 10 samples were collected from both lakes with each 5 representing each lake. Identification and Isolation of gram-negative bacteria was performed through culture of pleco's liver swab results in MacConkey media using gram staining method and were continued by biochemical testing using VITEK 2 System. The acquired results indicated that there were 8 different species of gram-negative bacteria isolated and all of them were pathogenic to the plecos. The species of bacteria includes Klebsiella pneumoniae, E.coli, proteus sp., Acinetobacter baumannii, Aeromonas sobria, Enterobacterb cloacae complex, Citrobacter freundii, Enterobacter aerogenes. Specimen preparation for liver histopathology was carried out by 10% neutral buffered formalin (NBF) fixation, graded ethanol series dehydration, paraffin wax embedding, 4 μm sectioning, Hematoxylin and Eosin Staining. After that, the specimen was observed under a microscope. Data analysis employed qualitative descriptive method. From the observation, liver damages such as necrosis, inflammatory cell infiltration, hemorrhage, and cell hyperthrophy were identified. Liver damages were most probably caused by the infection of gram-negative bacteria. Keywords : Sidenreng Lake, Lapompakka Lake, Liver, Histopathology, Amazon Sailfin Catfish
Effectiveness of Ginger Ointment (Zingiber officinale roscoe) on Incision Wound Healing in White Rats (Rattus norvegicus) Abdul Wahid Jamaluddin; Ayu Lestari; Wa Ode Santa Monica
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 5 No. 1, JANUARY 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v5i1.13060

Abstract

Ginger (Zingiber officinale roscoe) is one of the most popular plants to be processed into herbal medicine. This plant contains active compounds in the form of gingerols, shogaols, flavonoids and saponins, which have anti-inflammatory, anti-tumor, antioxidant and antimicrobial effects. This study aims to determine the effectiveness of ginger extract ointment on incision wound healing time in white rats (Rattus norvegicus) by observing the percentage of wound healing and changes in wound morphology. This study used a laboratory experimental method using 24 white rats as test animals which were divided into four groups, namely two treatment groups (10% and 20% ginger extract ointment) and two control groups (one negative control group (no treatment) and one positive control group (Oxyfresh Soothing Pet Gel®)). The rats were anesthetized and shaved, then an incision was made on the dorsal part of the rat. The treatment was given twice a day for 14 days. The results showed that there were differences in the comparison of the percentage of wound area between the negative control group (without treatment), the positive control (Oxyfresh Soothing Pet Gel®), the treatment groups (ginger extract ointment 10% and 20%). The conclusion of the study was that the treatment group with 10% ginger extract ointment was more effective in accelerating the wound healing process. Keywords: Ginger extract,  Incision, wound healing, ointment, white rat.
Haematological Profile of Fracture Rabbit with Horse Bone Graft Implant Zulfikri Mustakdir; Wa Ode Santa Monica; Ulfa Desianti Liding; Muhammad Zulfadhillah Sinusi
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, January 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.27073

Abstract

Fractures are commonly find in the world of veterinary practitioners. Fractures do not only occur as a result of accidents, but can also occur due to bone diseases or due to an imbalance nutrition in animals. One method that can be used to increase the healing process is through bone implantation. Bone graft is a material to speed up the bone healing process because it can provide mechanical support and signals to the body's molecules to maximize tissue growth. Bone implants that are often used and commonly found are bovine bone implants. Another implant material that we can use is horse bone. Bone grafts from horse bones have not been widely studied. Horse bone has the potential to be used as bone graft material because it has a more compact and harder structure. The aim of this study was to asseess the haematological profile of fracture rabbit with horse bone graft. The experimental animal used was a rabbit. A total of 12 rabbits were divided into three groups, namely the control group, the group with horse bone graft, and the group with commercial bone graft. Horse bone graft is obtained from horse bone waste through sintering and hydroxyapatite synthesis processes. Blood collection was performed pre-surgery, week 2 and week 6 postoperatively to see the response and description of blood cells against bone graft. The results of hemoglobin and erythrocyte examinations showed no significant difference between the three groups (P<0.05). Hemoglobin level in rabbits implanted with horse bone grafts was still at normal levels, except for the erythrocyte levels which were slightly below normal after surgery.
Co-Authors Abdul Wahid Jamaluddin Andi Magfira Satya Apada Andi Rianti Rhasinta Ayu Lestari Hairul Afzan Muhammad Zulfadhillah Sinusi Ulfa Desianti Liding Zulfikri Mustakdir