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All Journal Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Ilmu Ternak dan Veteriner Transformasi
Subiyanto .
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Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Languange, Linguistic, Communication & Media Mathematics Veterinary

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Journal : Jurnal Ilmu Ternak dan Veteriner

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The preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresis Gholib, Djaenudin; ., Subiyanto
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 3 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (695.175 KB) | DOI: 10.14334/jitv.v3i3.117

Abstract

The filtrate antigen ofDermatophilus congolensis was prepared based on the Makinde method, whereas the whole cell antigen was based on the Bida and Kelley method. Filtrate antigen of Dermatophilus congolensis has been tested with positive serum from experimental animals, guinea pigs and sheep by means of immurrodiffitsion test and electrophoresis . Positive serum was produced by inoculation of whole cell antigen of D. congolensis to the animals . The results showed that the immunodiffusion test resulted in one and two precipitation lines with positive serum of sheep and guinea pigs respectively. Electrophoresis SDS-PAGE presented about 8 bands with molecular weight in the range from above 30 kD to more than 94 kD. The bands were then transferred into nitrocellulose membrane and gave positive reaction with positive serum from sheep.   Key words : Antigen, Dermatophilus congolensis, immunodiffusion, electrophoresis
The preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresis Djaenudin Gholib; Subiyanto .
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 3 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (695.175 KB) | DOI: 10.14334/jitv.v3i3.117

Abstract

The filtrate antigen ofDermatophilus congolensis was prepared based on the Makinde method, whereas the whole cell antigen was based on the Bida and Kelley method. Filtrate antigen of Dermatophilus congolensis has been tested with positive serum from experimental animals, guinea pigs and sheep by means of immurrodiffitsion test and electrophoresis . Positive serum was produced by inoculation of whole cell antigen of D. congolensis to the animals . The results showed that the immunodiffusion test resulted in one and two precipitation lines with positive serum of sheep and guinea pigs respectively. Electrophoresis SDS-PAGE presented about 8 bands with molecular weight in the range from above 30 kD to more than 94 kD. The bands were then transferred into nitrocellulose membrane and gave positive reaction with positive serum from sheep.   Key words : Antigen, Dermatophilus congolensis, immunodiffusion, electrophoresis
Co-Authors Anang Najamuddin Djaenudin Gholib Djaenudin Gholib Sulistiono