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Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp.) semen obtained by electroejaculation ., Yudi; Yusuf, T.L.; Purwantara, B; Sajuthi, D.; Agil, M
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.463 KB) | DOI: 10.14334/jitv.v16i1.633

Abstract

The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor). Semen was collected from 2 anesthetized males (4-10 years) by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes), and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa). The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05) during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program. Key Words: Anoa, Seminal Plasma, Extended Semen, Frozen Semen, Electroejaclator
The viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extender ., Yudi; Arifiantini, I; Purwantara, B; Yusuf, T.L
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.697 KB) | DOI: 10.14334/jitv.v13i1.593

Abstract

The objective of the experiment was to study the motility and viability of spermatozoa of fresh semen, and the quality of extended semen with different sperm concentration in Dimitropoulos-modified extender. Semen was collected using artificial vagina from three 4-8 year old stallions (different breed). Semen characteristics and quality was evaluated macro- and microscopically. For longevity evaluation, semen was stored at room and chilled temperature, and was evaluated for motility and viability every 3 h. Prior extension, semen was centrifugated at 3000 rpm for 20 minutes. The condensed sperm was re-suspended in Dimitropoulos (DV) supplemented with 50 mM fructose with the concentration of 200, 100 and 50 x 106 spz/mL. All samples were stored at room and chilled temperature, and was evaluated for motility and viability every 3 h and 12 h for room and chilled temperature. Results of the experiments indicated that fresh semen characteristics was fairly good. For longevity evaluation, semen with motility of 48.33 and 10.42% was observed at 3 h and 12 h after the onset of storage. The extended-semen with 50 x 106 spz/mL showed significantly higher in term of motility and viability (P<.05) than that with 200 x 106 spz/mL, but for that of 100 x 106 spz/mL. It is recommended that sperm concentration should be 50 x 106 spz/ml for a long period storage with reasonable good quality. Key words: Stallion, Semen, Sperm Concentration, Dimitropoulos
Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp.) semen obtained by electroejaculation Yudi .; T.L. Yusuf; B Purwantara; D. Sajuthi; M Agil
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.463 KB) | DOI: 10.14334/jitv.v16i1.633

Abstract

The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor). Semen was collected from 2 anesthetized males (4-10 years) by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes), and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa). The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05) during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program. Key Words: Anoa, Seminal Plasma, Extended Semen, Frozen Semen, Electroejaclator
The viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extender Yudi .; I Arifiantini; B Purwantara; T.L Yusuf
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.697 KB) | DOI: 10.14334/jitv.v13i1.593

Abstract

The objective of the experiment was to study the motility and viability of spermatozoa of fresh semen, and the quality of extended semen with different sperm concentration in Dimitropoulos-modified extender. Semen was collected using artificial vagina from three 4-8 year old stallions (different breed). Semen characteristics and quality was evaluated macro- and microscopically. For longevity evaluation, semen was stored at room and chilled temperature, and was evaluated for motility and viability every 3 h. Prior extension, semen was centrifugated at 3000 rpm for 20 minutes. The condensed sperm was re-suspended in Dimitropoulos (DV) supplemented with 50 mM fructose with the concentration of 200, 100 and 50 x 106 spz/mL. All samples were stored at room and chilled temperature, and was evaluated for motility and viability every 3 h and 12 h for room and chilled temperature. Results of the experiments indicated that fresh semen characteristics was fairly good. For longevity evaluation, semen with motility of 48.33 and 10.42% was observed at 3 h and 12 h after the onset of storage. The extended-semen with 50 x 106 spz/mL showed significantly higher in term of motility and viability (P<.05) than that with 200 x 106 spz/mL, but for that of 100 x 106 spz/mL. It is recommended that sperm concentration should be 50 x 106 spz/ml for a long period storage with reasonable good quality. Key words: Stallion, Semen, Sperm Concentration, Dimitropoulos
Co-Authors B Purwantara B Purwantara D. Sajuthi D. Sajuthi I Arifiantini M Agil T.L Yusuf T.L. Yusuf T.L. Yusuf