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All Journal Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture TREUBIA
D. Sajuthi
Department of Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine Bogor Agricultural University, Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education Veterinary

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Molecular characterization of Bovine herpesvirus type 1 Indonesian isolates Saepulloh, Muharam; Wibawan, I.W.T.; Sajuthi, D.; Setiyaningsih, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.846 KB) | DOI: 10.14334/jitv.v14i1.365

Abstract

Different subtypes of bovine herpesvirus 1 (BHV-1) have been associated with different clinical conditions of cattle. For that reason subtypes differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BHV infections. In search for a genomic region that would allow a clear distinction between BHV-1.1 and BHV-1.2 of glycoprotein D (gD) genes of 8 Indonesian isolates were amplified and sequenced. The amino acid sequence alignments revealed that the levels of genomic similarity ranging from 98.8 to 100% among BHV-1 Indonesian isolates and its results were also similar between BHV-1 Indonesia isolates and BHV-1.1 reference, and 98.4 to 98.8% between BHV-1 Indonesian isolates and BHV-1.2 reference. The isolates could be clearly separated into BHV-1.1 and BHV-1.2 after phylogenetic analysis. The results showed that the Indonesian isolates were characterized as BHV-1.1 as agent caused respiratory tract infections in cattle or infectious bovine rhinotracheitis (IBR) disease. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses. Key Words: BHV-1.1, BHV-1.2, Glycoprotein D, Phylogenetic Analysis, IBR
Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp.) semen obtained by electroejaculation ., Yudi; Yusuf, T.L.; Purwantara, B; Sajuthi, D.; Agil, M
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.463 KB) | DOI: 10.14334/jitv.v16i1.633

Abstract

The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor). Semen was collected from 2 anesthetized males (4-10 years) by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes), and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa). The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05) during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program. Key Words: Anoa, Seminal Plasma, Extended Semen, Frozen Semen, Electroejaclator
Extraction and isolation of Ovine Pregnancy-Associated Glycoprotein (ovPAG) from cotyledon placenta of Garut sheep Setiatin, E.T.; Sajuthi, D.; Purwantara, B.; Talib, C.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.806 KB) | DOI: 10.14334/jitv.v14i3.342

Abstract

Pregnancy-associated glycoprotein (PAG) structurally related to aspartic protease, expressed in the outer epithelial cell layer (trophectoderm) of ungulate placenta. Ovine PAG (ovPAG) synthesized by mono- and binucleic trophoblast before complete implantation at Day 14-15. Of this, ovPAG could be used as a marker for early pregnancy. The objective of study was to extract and isolate PAG from placenta of Garut Sheep collected at term and to characterize their molecular weight. The procedures included extraction of protein at neutral pH (cotyledon was thawed, minced, added PBS, blended and centrifuged), acidic (H3PO41M, pH 4,5; centrifuged) and ammonium sulfate (40% and 80% (NH4)SO4, centrifuged) precipitation; gel filtration (Sephadex-G75), anion exchange chromatography (DEAE- cellulose). Cotyledon extract was subjected to Sephadex-G75 and DEAE cellulose, and their fractions were measured their absorbances. Absorbances of Sephadex-G75 and DEAE fractions at peak were assayed for protein concentration (Bichinconinic protein assay). Continuously, these fractions were subjected to monogel SDS-PAGE and stained by Commassie Brilliant Blue. It was four different molecular weights isolating from cotyledon of Garut Sheep, namely 68.8, 36.04, 32.39 and 12.18  kDa. However, after chromatography anion exchange (DEAE-cellulose), only  three bands exist, those were  71,67;  33,64 and 30,86 kDa. Key words: Garut sheep, Pregnancy-Associated Glycoprotein (PAG), Cotyledone, SDS-PAGE, DEAE-cellulose
MORPHOLOGICAL VARIATION IN THE EBONY AND SILVER LEAF MONKEYS [TRACHYPITHECUS AURATUS (E. GEOFFROY, 1812) AND TRACHYPITHECUS CRlSTATUS (RAFFLES, 1821)] FROM SOUTHEAST ASIA MARYANTO, IBNU; MANSJOER, I.; SAJUTHI, D.; SUPRlATNA, J.
TREUBIA Vol 31, No 2 (1997): Vol. 31 No. 2, December 1997
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9082.567 KB) | DOI: 10.14203/treubia.v31i2.615

Abstract

The morphological variation in the ebony and silver leaf monkeys (Trachypithecus auratus and T. cristatus) from Southeast Asia; Thailand, Malaya, Bintan, Sumatra, Serasan-Natuna, Kalimantan and Java were studied using multivariate analysis approaches. The results showed that clinal variation in skull; dental and dentary morphology was found in Java. The skull; dental and dentary characters showed an increase from West to East with Central Java as an intermediate fonn. Consequently, in Java there should be only one subspecies, T. a. auratus. Meanwhile, there are fourmorpholgical groups of Trachipithecus cristatus, those are of Thailand, Malaya-Sumatra-Kalimantan, Bintan and Natuna. The Malayan population, presumably is the same as Bintan population while Sumatra-Kalimantan forms an intermediate.
ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG) Setiatin, E.T.; Sajuthi, D.; Purwantara, B.; Talib, C.; Fathul, F.; Adiati, U.; Praira, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.159-165

Abstract

The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG) which could detectPAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-TrisHCl (DT),DEAE-NaCl 20mM (DN2), DEAE-NaCl 40mM (DN4), DEAE-NaCl 80mM (DN8), DEAE-NaCl160mM (DN16), DEAE-NaCl 320mM (DN32) and DEAE-NaCl 1M (DN1) and NaCl 0.9 % as aplacebo. The 0.5 ml of isolate (purified from ovine cotyledon) was emulsified in equal volume withcomplete and incomplete Freud’s adjuvant. The mixture of each isolate and adjuvant was injected atmutiple sites along the dorsal area of rabbits by subcutaneous route. Blood were collected from marginalear vein, starting before first injection (baseline) and every 14 days. Rabbit anti-ovPAG were measuredusing Modified ELISA Technique. By using Western Blot Technique, DN32 showed the best immuneresponse among others and also could differenciate ovPAG in the urine of pregnant ewes It could beconcluded that ovPAG DN32 is a specific source of rabbit anti-ovPAG production. Protein of ovPAG atmolecular weight 31 kDa is a pregnancy protein marker of garut sheep and could be developed as amajor protein for producing antibodi.
EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION Arifiantini, R.I.; Purwantara, B.; Yusuf, T.I.; Sajuthi, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 1 (2010): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.1.68-74

Abstract

s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos(DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethyleneglycol-glycerol (EG+Gly) or dimethilformamide (DMF). Each semen sample was evaluated and dividedequally into six tubes; semen in the three tubes was diluted 1:1 with (SM), while in the remaining tubesthe semen was diluted 1:1 by DV. After being diluted, all tubes were centrifuged at 1006xg for 10minutes. The supernatan discarded, the pellet was rediluted by SM trehalosa or DV trehalose, and addedby G, EG+Gly, or DMF to reach the final sperm concentration of 200x106/ml. The extended semen wasindividually packed in 0.3 ml minitube, equilibrated at 4oC for 2 hours, frozen in liquid nitrogen vaporfor 10 minutes, and then was stored in liquid nitrogen container at -196 oC. After 24 hours, the semenwas thawed at 37 oC for 30 second. There were no significantly different (p>0.05) on the percentages ofmotile and viable sperm in SMT (21.7% and 43.4%, respectively) compared with those extended withDV T extender (26.9% and 50.8%, respectively). DMF demonstrated better results as CPA compared tothe others; and DVTDMF combination had the best protection during cryopreservation in this study.
Co-Authors B Purwantara B. Purwantara C. Talib D. Setiyaningsih E.T. Setiatin F. Fathul I. MANSJOER, I. I.W.T. Wibawan Ibnu Maryanto J. SUPRlATNA, J. M Agil Muharam Saepulloh R.I. Arifiantini T.I. Yusuf T.L. Yusuf U. Adiati W. Praira Yudi .