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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak dan Veteriner Policy Brief Pertanian, Kelautan, dan Biosains Tropika

M Agil

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Author-ID : 221081

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Social Sciences Veterinary

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Articles

Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp.) semen obtained by electroejaculation ., Yudi; Yusuf, T.L.; Purwantara, B; Sajuthi, D.; Agil, M
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor). Semen was collected from 2 anesthetized males (4-10 years) by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes), and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa). The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05) during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 Â± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program. Key Words: Anoa, Seminal Plasma, Extended Semen, Frozen Semen, Electroejaclator

Morfologi dan Biometri Spermatozoa Anoa (Bubalus Sp.) yang Diwarnai dengan Pewarna William’s dan Eosin-Nigrosin Yudi Yudi; T L Yusuf; B Purwantara; M Agil; T Wresdiyati; D Sayuthi; Aditya Aditya; J Manansang; R Sudarwati; Y T Hastuti
Media Peternakan Vol. 33 No. 2 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Anoa is Indonesia endemic fauna that included into Appendix I by CITES. Anoa breeding efforts have not succeed, due to their agressivity, soliter, wildness and monogamous. The aim of this experiment was to observe morphology and biometry of anoa's sperm stained using William's (W) and eosin-nigrosin (EN). Semen was collected from two male anoas by electroejaculator. Slides was made for morphology and biometry observation of ejaculate. The results showed that abnormal sperm morphology of the mature anoa (10 years) was 32.27%-35.00%, meanwhile the young one (3 years) was 24.03%-31.89%. In mature anoa, measurement for head width, and midpiece and tail length of sperm were not signficantly different between W and EN staining, but significantly different (P < 0.05) for head and total length. In the young anoa, sperm biometry for head width was not significanly different between W and EN staining. In both W and EN staining, biometry for head width and total length were not significantly different between mature and young anoas, but significantly different for head, midpiece and tail length. According to sperm morphology, semen from the young anoa was slightly better than those of the mature anoa. Staining method and age could affect to biometry of the anoa's sperm.

Successful Intracervical Insemination and Characteristics of Anoa (Bubalus sp.) Parturation Behavior in Captivity . Judi; T L Yusuf; B Purwantara; D Sajuthi; M Agil; J Manansang; R Sudarwati; Y T Hastuti; B Huoso; A Widianti; S Prastiti
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Anoa, which is the endemic animal in Indonesia, its population is unevitably decreasing, therefore anoa included in the list of endangered species by International Union for Conservation of Nature (IUCN). The experiment was aimed to apply the artificial insemination (AI) technique for anoa and to examine their parturition behavior. The experiment involved 2 males and 5 females anoa at Taman Safari Indonesia Bogor. Semen was collected by electroejaculator, then evaluated and diluted in Tris-egg yolk extender. The anesthetized estrous females were inseminated intracervically with extended semen (100x106 sperm/1.0 mL). Females which did not show estrous signs during 2-3 cycles after AI were predicted pregnant, and therefore confirmed by ultrasonography. As a comparison, another predicted-pregnant female after natural mating was scanned. The results showed that intracervical AI resulted pregnancy in one female, and the gestation period of anoa was ranged from 313 d (AI) to 324 d (natural mating). There were three stages of parturition process was observed: Stage 1 was characterized by the abdominal contraction and amniotic membrane rupture, Stage 2 was characterized by the fetal expulsions, and Stage 3 was characterized by the placental expulsions and ingestion of placenta by the dam. The duration of each stage of parturition was 6-8 h (stage 1), 30-60 min (stage 2), and 15-180 min (stage 3). It is concluded that AI technique was applicable to anoa in captivity, and the parturation behavior of anoa was comparable to buffalo and cattle. However, the fetal delivery occurred when the dam was in standing position and the dam ingested the placenta.

Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp.) semen obtained by electroejaculation Yudi .; T.L. Yusuf; B Purwantara; D. Sajuthi; M Agil
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor). Semen was collected from 2 anesthetized males (4-10 years) by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes), and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa). The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05) during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program. Key Words: Anoa, Seminal Plasma, Extended Semen, Frozen Semen, Electroejaclator

Pengembangan Uji Berbasis Molekuler Protamine-1 (Prm1) Sebagai Penentu Tingkat Fertilitas untuk Proses Seleksi dan Kebijakan Pengafkiran Sapi Pejantan Unggul di Indonesia B Purwantara; BP Pardede; M Agil; I Supriatna; NWK Karja; RI Arifiantini; C Sumantri
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 4 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.4.4.367-374

Standar kualitas semen yang berperan penting dalam keberhasilan inseminasi buatan (IB) sebagai upaya peningkatan populasi dan mutu ternak sapi di Indonesia terbukti tidak seutuhnya mampu menentukan tingkat fertilitas sapi pejantan di Indonesia. Studi terbaru membuktikan bahwa meskipun sudah memenuhi standar kualitas semen yang sudah ditetapkan, namun tiga dari enam ekor sapi pejantan di Indonesia masih tergolong fertilitas rendah (<60%). Potamine-1 (PRM1) merupakan molekul pada inti spermatozoa yang telah terbukti memiliki hubungan erat dengan fungsi fertilitas dan kualitas spermatozoa pada berbagai sapi pejantan di Indonesia. Hal tersebut mendasari usulan pengembangan uji berbasis molekuler PRM1 sebagai penentu tingkat fertilitas yang diharapkan dapat digunakan untuk proses seleksi dan kebijakan pengafkiran sapi pejantan unggul di Indonesia. Melalui policy brief ini diharapkan usulan pengembangan uji tersebut dapat lebih tersampaikan dengan segala solusi yang diberikan, termasuk terkait pendekatan uji berbasis molekuler yang paling tepat digunakan, kajian-kajian lanjutan, dan berbagai harapan dukungan dari segala pihak demi terwujudnya pengembangan uji PRM1.

Co-Authors . Judi A Widianti Aditya Aditya B Huoso B Purwantara B Purwantara BP Pardede C Sumantri D Sajuthi D Sayuthi D. Sajuthi D. Sajuthi I Supriatna J Manansang NWK Karja R Sudarwati RI Arifiantini S Prastiti T L Yusuf T Wresdiyati T.L. Yusuf T.L. Yusuf Y T Hastuti Yudi . Yudi Yudi

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