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All Journal Buletin Peternakan Microbiology Indonesia
Ali Wibowo
Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Pengaruh Penggunaan Kerabang Telur dalam Ransum terhadap Kadar Kalsium di Mukosa Usus Halus, Plasma Darah, Tulang Tibia dan Ekskreta Ayam Petelur Adi Ratriyanto; Tri Yuwanta; Ali Wibowo
Buletin Peternakan Vol 25, No 1 (2001): Buletin Peternakan Vol. 25 (1) Februari 2001
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v25i1.1424

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Pengaruh Penambahan Vitamin C dalam Ransum Menggunakan Tepung Darah terhadap Absorpsi Fe pada Ayam Broiler Stelly Novaria Rumerung; Ali Wibowo
Buletin Peternakan Vol 23, No 2 (1999): Buletin Peternakan Vol. 23 (2) Mei 1999
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v23i2.1649

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Penentuan Daya Tetas dengan Menggunakan Metode Gravitasi Spesifik pada Tingkat Berat Inisial Ayam Kampung yang Berbeda Ali Wibowo; Tri Yuwanta; Jafendi H. P. Sidadolog
Buletin Peternakan Vol 18, No 4 (1994): Buletin Peternakan Vol. 18 (4) Desember 1994
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v18i4.1719

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Mutagenic Improvement of Xylanase Production from Xylanolytic Bacteria and its Phylogenetic Analysis CHUSNUL HANIM; LIES MIRA YUSIATI; MUHAMMAD NUR CAHYANTO; ALI WIBOWO
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.232 KB) | DOI: 10.5454/mi.7.2.2

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This study was conducted to obtain xylanolytic mutants that have higher xylanase activity than their wildtype counterparts. A mutant with the best xylanolytic activity was selected and identified based on its 16S rRNA sequence. Its optimum growth condition was also characterized and its phylogenetic relations to other xylanolytic bacteria were analzsed. Wild type xylanolytic alkalophlic bacteria were grown in medium containing xylan as a substrate. Mutation was performed using ethidium bromide (EtBr) or ethyl methanesulfonate (EMS) atconcentrations 50, 100, and 150 mg mL-1 and times of exposure 30, 60, 90, and 120 min for each treatment. Twenty two mutants were obtained from EtBr and 24 mutants from EMS mutageneses. The mutants were analyzed for their capability to secrete xylanase into xylan medium containing xylose or glucose or glycerol. Growth optimizations of the mutant were done in media with pH range 6-11 and temperature range 30 to 60 °C. Mutant number 19, which was obtained by treatment using 50 mg mL-1 EMS for 120 min, had the highest xylanase activity (15.057 U g-1). This activity was obtained at optimum growth conditions: pH 9.5 and temperature 55 °C. Chromosomal DNA of this mutant was extracted and amplified by PCR using 16S rRNA gene specific primers. The amplified fragments were sequenced by dideoxynucleotide chain terminator method. The phylogenetic analysis based on 16S rRNA gene sequence showed that mutant 19 was closed to an anaerobic xylanase producing bacteria.
Co-Authors Adi Ratriyanto Chusnul Hanim Jafendi H. P. Sidadolog Lies Mira Yusiati Muhammad Nur Cahyanto Stelly Novaria Rumerung Tri Yuwanta