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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia Buletin Peternakan International Journal of Renewable Energy Development Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati agriTECH Indonesian Journal of Biotechnology Bioeksperimen: Jurnal Penelitian Biologi JITEK (Jurnal Ilmiah Teknosains) Indonesian Food and Nutrition Progress Jurnal Teknologi Pertanian (Agricultural Technology Journal) Canrea Journal: Food Technology, Nutritions, and Culinary Journal Jurnal Pengendalian Pencemaran Lingkungan (JPPL)
Muhammad Nur Cahyanto
Departemen Teknologi Pangan Dan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Jalan Flora, Bulaksumur, Yogjakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Veterinary Other

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Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa Suyanto, Eko; Soetarto, Endang Sutariningsih; Cahyanto, Muhammad Nur
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: Maret 2015
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Lipase memiliki manfaat penting di bidang industri. Tujuan penelitian ini adalah untuk mendapatkan kapang lipolitik yang mampu tumbuh dan menghasilkan aktivitas lipase tinggi pada limbah ampas kelapa menggunakan metode solid state fermentation. Isolat kapang uji dipurifikasi kemudian dilakukan skrining dan seleksi kapang lipolitik dan dilanjutkan dengan produksi lipase menggunakan substrat ampas kelapa yang sebelumnya diukur kandungan biokimia. Hasil menunjukkan bahwa 8 isolat kapang lipolitik mampu tumbuh baik pada substrat ampas kelapa yang ditunjukkan dengan adanya sporulasi dan perubahan pH medium selama reaksi. Diantara kapang lipolitik tersebut, isolat kapang KLC-333 diketahui menghasilkan aktivitas hidrolisis lipase terbesar yaitu 13,33 U/ml dan volume produksi 46 ml. Biosintesis dan peningkatan produksi lipase dipengaruhi oleh kandungan nutrien di dalam substrat ampas kelapa.
Effects of Dissolved Oxygen Tension and Ammonium Concentration on Polyhydroxybutyrate Synthesis from Cassava Starch by Bacillus cereus IFO 13690 ., Margono; ., Rochmadi; Syamsiah, Siti; Cahyanto, Muhammad Nur
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Attempting to get low price of raw material for producing polyhydroxybutyrate is always studied. Tapioca starch is one of the raw material with low price. The objective of this research was to study the effects of initial ammonium concentration and dissolved oxygen tension (doT) on producing PHB by Bacillus cereus IFO 13690 with tapioca starch as the carbon source. This fermentation was carried out in 5 L fementors with a 2 L working volume, temperature of 30 oC, and agitation of 500 rpm. The pH medium was controlled at 5.6 after it came down from the initial pH of 6.8. Meanwhile, the initial doT was 100 % air saturation and also came down to and maintained at doT of experiment, i.e. 1 , 5 , or 10 % air saturation. The best result was obtained when the initial ammonium concentration was 5 g/L and the doT value maintained at 5 % air saturation. By this conditions, the cell growth reached 5,457 g cell dry weight/L containing PHB of 2.42 % cell dry weigh after 29 hours fermentation.
PEMANFAATAN HASIL FERMENTASI WHEY TAHU MENGGUNAKAN ISOLAT PEDIOCOCCUS ACIDILACTICI F11 SEBAGAI ALTERNATIF KOAGULAN PADA PEMBUATAN TAHU K. Sya’di, Yunan; Rahayu, Endang Sutriswati; Cahyanto, Muhammad Nur
Jurnal Ilmiah Teknosains Vol 1, No 1/November (2015): JITek
Publisher : Jurnal Ilmiah Teknosains

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Abstract

Pembuatan tahu di Indonesia banyak menggunakan kecutan sebagai penggumpal. Kecutan ini memiliki kelemahan karenajenis dan jumlah mikrobia yang berperan dalam fermentasi dapat berbeda dari satu fermentasi dengan fermentasi lainnya.Akibatnya mutu tahu yang dihasilkan dari proses penggumpalannya juga kurang stabil. Penelitian ini bertujuan menggunakanhasil fermentasi whey tahu dengan bakteri asam laktat, Pediococcus acidilactici F11 sebagai alternatif penggumpal pada skalaindustri. Fermentasi dilakukan pada fermentor 125 L selama 5 hari produksi (5 batch) pada skala industri. Populasi akhirbakteri asam laktat dari 5 batch fermentasi kecutan menggunakan Pediococcus acidilactici F11 pada fermentor 125 liter selama16 jam memiliki rata-rata 1,19 x 109 CFU/mL, sedangkan bakteri non BAL dan coliform memiliki rata-rata 1,8 x 102 CFU/mLdan 8,23 CFU/mL. Keasaman dan pH pada akhir fermentasi kecutan sudah memenuhi persyaratan sebagai penggumpaldengan memiliki rata-rata 3,54 g/L dan pH rata-rata 3,94. Berat tahu yang dihasilkan dari koagulan hasil fermentasi whey tahumenggunakan Pediococcus acidilactici F11 lebih tinggi 5,9% dibanding menggunakan koagulan dari kecutan.
Karakterisasi, Identifikasi dan Uji Aktivitas Anti Jamur Minyak Atsiri Daun Jeruk Purut (Citrus hystrix) terhadap Aspergillus Yanti, Rini; Wulandari, Pudji; Pranoto, Yudi; Cahyanto, Muhammad Nur
Jurnal Teknologi Pertanian (Agricultural Technology Journal Vol 8, No 2 (2017): Jurnal Teknologi Pertanian (Teta)
Publisher : Fakultas Pertanian, Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jteta.8.2.2017.18700

Abstract

The objective of the work was to observe the physical properties, identify the components, and determine the antifungal activity of essential oil from lime leaves. Essential oil was extracted from lime leaves by water-steam distillation and determined its physical properties such as specific gravity, refractive index, and solubility in alcohol. The components of the oil were analyzed using GC-MS. Antimicrobial activity was evaluated against five species of Aspergillus, i.e.:A. niger, A.flavus, A.tamarii, A.Parasiticus, and A. candidus. Spores of the fungi were inoculated into plates of potato dextrose agar supplemented with lime leave essential oil at different concentration and incubate at 30oC for 7 days.  Development of colonies was observed during the incubation. The essential oil from lime leaves had specific gravity of 0.8601, refractive index of 1.4523, and soluble in 2 volume and more 90% alcohol.The main compound in the essential oil was citronellal (87.9%). The oil also contained linalool (3.96%), transcaryophylene (2.17%), citronellil acetat (1.79%), and sabinene (1.78%). The oil showed antimicrobial activity against these five species of Aspergillus.Keywords: Lime leaves, essential oil, antifungal, Aspergillus
Produksi Bioetanol Daun Sorghum (Sorghum bicolor L.Moench) Ria Millati, Birgitta Narindri Muhammad Nur Cahyanto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 1 (2016): February 2016
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.374 KB) | DOI: 10.24002/biota.v1i1.712

Abstract

Penelitian ini bertujuan menganalisis  potensi daun sorghum (Sorghum bicolor L.  Moench) sebagai biomassa dalam produksi etanol. Daun sorghum akan digunakan sebagai substrat dalam proses hidrolisis enzimatis dan gula reduksi yang dihasilkan akan digunakan sebagai substrat dalam fermentasi. Hidrolisis dilakukan tanpa perlakuan pendahuluan. Daun sorghum dihancurkan hingga berbentuk serbuk berukuran 30mesh sebelum digunakan sebagai substrat dalam hidrolisis enzimatis menggunakan enzim Celluclast (novozyme) 90FPU/g berat kering. Konsentrasi gula reduksi dan nilai CCR (Cellulose Conversion Ratio) akan digunakan untuk menganalisis  tingkat degradabilitas selulosa daun sorghum. Gula reduksi dalam hidrolisat sebagai hasil proses hidrolisis enzimatis akan difermentasi oleh Saccharomyces cerevisiae FNCC 3012 untuk menghasilkan etanol. Konsentrasi  Sorghum  cerevisiae  yang digunakan adalah 106 CFU/ml dan ditambahkan dalam 10% volume hidrolisat. Pengamatan dalam proses fermentasi dilakukan terhadap konsentrasi gula reduksi dan etanol selama 36 jam. Hasil yang didapatkan menunjukkan bahwa selulosa tidak dapat dihidrolisis enzimatis secara langsung dilihat dari rendahnya nilai CCR. Konsentrasi gula reduksi tertinggi setelah hidrolisis enzimatis adalah 3,18 mg/ml. Konsentrasi etanol tertinggi setelah proses fermentasi adalah 0,23%(v/v).
Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa Suyanto, Eko; Soetarto, Endang Sutariningsih; Cahyanto, Muhammad Nur
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: Maret 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i1.311

Abstract

Lipase memiliki manfaat penting di bidang industri. Tujuan penelitian ini adalah untuk mendapatkan kapang lipolitik yang mampu tumbuh dan menghasilkan aktivitas lipase tinggi pada limbah ampas kelapa menggunakan metode solid state fermentation. Isolat kapang uji dipurifikasi kemudian dilakukan skrining dan seleksi kapang lipolitik dan dilanjutkan dengan produksi lipase menggunakan substrat ampas kelapa yang sebelumnya diukur kandungan biokimia. Hasil menunjukkan bahwa 8 isolat kapang lipolitik mampu tumbuh baik pada substrat ampas kelapa yang ditunjukkan dengan adanya sporulasi dan perubahan pH medium selama reaksi. Diantara kapang lipolitik tersebut, isolat kapang KLC-333 diketahui menghasilkan aktivitas hidrolisis lipase terbesar yaitu 13,33 U/ml dan volume produksi 46 ml. Biosintesis dan peningkatan produksi lipase dipengaruhi oleh kandungan nutrien di dalam substrat ampas kelapa.
Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste Amekan, Yumechris; Wangi, Dyah Sekar A P; Cahyanto, Muhammad Nur; Sarto, Sarto; Widada, Jaka
International Journal of Renewable Energy Development Vol 7, No 2 (2018): July 2018
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.7.2.101-109

Abstract

The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of microbes that induce biogas formation, so this study examined the effect of different inoculum source and its combination of biohydrogen production performance. The hydrogen producing bacteria from fruit waste digester (FW), cow dung digester (CD), and tofu waste digester (TW) enriched under strictly anaerobic conditions at 37OC. Inoculums from 3 different digesters (FW, CD, and TW) and its combination (FW-CD, CD-TW, FW-TW, and FW-CD-TW) were used to test the hydrogen production from melon waste with volatile solids (VS) concentration of 9.65 g/L, 37°C and initial pH 7.05 ± 0.05. The results showed that individual and combined inoculum produced the gas comprising hydrogen and carbon dioxide without any detectable methane. The highest cumulative hydrogen production of 743 mL (yield 207.56 mL/gVS) and 1,132 mL (yield 231.02 mL/gVS) was shown by FW and FW-CD-TW, respectively. Butyric, acetate, formic and propionic were the primary soluble metabolites produced by all the cultures, and the result proves that higher production of propionic acid can decrease hydrogen yield. Clostridium perfringens and Clostridium baratii prominently seen in all single and combination inoculum. Experimental evidence suggests that the inoculum from different biodigesters able to adapt well to the environmental conditions and the new substrate after a combination process as a result of metabolic flexibility derived from the microbial diversity in the community to produce hydrogen. Therefore, inoculum combination could be used as a strategy to improve systems for on-farm energy recovery from animal and plant waste to processing of food and municipal waste.Article History: Received February 5th 2018; Received in revised form May 7th 2018; Accepted June 2nd 2018; Available onlineHow to Cite This Article: Amekan, Y., Wangi, D.S.A.P., Cahyanto, M.N., Sarto and Widada, J. (2018) Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste. Int. Journal of Renewable Energy Development, 7(2), 101-109.https://doi.org/10.14710/ijred.7.2.101-10
Effect of Hydrogen Peroxide on Hydrogen Production from Melon Fruit (Cucumis melo L.) Waste by Anaerobic Digestion Microbial Community Kharisma, Agung Dian; Amekan, Yumechris; Sarto, Sarto; Cahyanto, Muhammad Nur
International Journal of Renewable Energy Development Vol 11, No 1 (2022): February 2022
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2022.40883

Abstract

Biohydrogen (H2) production has the potential to provide clean, environmentally friendly, and cost-effective energy sources. The effect of increasing oxidative stress on biohydrogen production by acid-treated anaerobic digestion microbial communities was studied. The use of varying amounts of hydrogen peroxide (H2O2; 0.1, 0.2, and 0.4 mM) for enhancing hydrogen production from melon fruit waste was investigated. It was found that H2O2 amendment to the H2-producing mixed culture increased hydrogen production. Treatment with 0.4 mM H2O2 increased cumulative H2 output by 7.7% (954.6 mL/L), whereas treatment with 0.1 mM H2O2 enhanced H2 yield by 23.8% (228.2 mL/gVS) compared to the untreated control. All treatments showed a high H2 production rate when the pH was 4.5 – 7.0.  H2O2-treated samples exhibited greater resilience to pH reduction and maintained their H2 production rate as the system became more acidic during H2 fermentation. The application of H2O2 affected the volatile fatty acid (VFA) profile during biohydrogen fermentation, with an increase in acetic and propionic acid and a reduction in formic acid concentration. The H2O2 treatment positively affects H2 production and is proposed as an alternative way of improving H2 fermentation.
AKTIVITAS ANTIOKSIDAN ANTOSIANIN BERAS KETAN HITAM SELAMA FERMENTASI [Antioxidant Activity of Anthocyanin of Black Glutinous Rice During Fermentation] Nanik Suhartatik; Muhammad Nur Cahyanto; Sri Raharjo; Endang S. Rahayu
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.215 KB) | DOI: 10.6066/jtip.2013.24.1.115

Abstract

Anthocyanin is a group of bioactive compound found to be abundant in black glutinous rice. It has been widely studied for their health beneficial effect. Hydrolysis of anthocyanin glycoside into anthocyanidin and sugar by β,D-glucosidase is presumed to be the first step in anthocyanin metabolism. Enzymatic degradation of anthocyanin was reported to produce not only more stable compounds, but also healthier compounds with better bioavailability. Some species of Lactic Acid Bacteria showed β,D-glucosidase activity. The research aims to study the functional property’s change’s of anthocyanin extracted from black glutinous rice as an antioxidant compound after being fermented using Lactobacillus plantarum Mut 7. The results showed that fermentation process did not give a significant effect to the antioxidant activity of black glutinous rice anthocyanin. The antioxidant activity as determined by Radical Scavenging Activity and Ferrous Reducing Activity Power value were 59.2% (6 hours of incubation, 30 mM anthocyanin) and 96.7% (5 hours of incubation, 10 mM anthocyanin). The lactic acid bacterial count increased up to 2 log cycle after being fermented for 5 hours.
Physical, Chemical, and Microbiological Characteristics of Ronto During Storage Rita Khairina; Muhammad Nur Cahyanto; Tyas Utami; Sri Rahardjo
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.783 KB) | DOI: 10.17844/jphpi.v19i3.15112

Abstract

AbstractThe purpose of this study was to determine the physical, chemical, and microbiological changes of ronto during storage at room and cold temperatures. Ronto was prepared by mixing fresh shrimp, salt, and rice with a ratio of 7 : 1 : 2 and fermented in a closed container for 14 days. Ronto produced was stored at room and cold (5oC) temperatures. Samples were withdrawn every 4 weeks and analyzed for its physical, chemical, and microbiological characteristics. The characteristic changes of ronto stored at low temperature were generally slower than those at room temperature. The changes in colour, viscosity, acidity, total volatile bases, and microbial counts were lower in ronto kept at 5oC compared to those in ronto kept at room temperature. The colour of ronto stored at low temperature remained red after 20 weeks, whereas the colour of ronto kept at room temperature turned brownish after 20 weeks.
Co-Authors Akhmad Mustofa Ali Agus Ali Agus Ali Mursyid Wahyu Mulyono Ali Wibowo Amekan, Yumechris Angga Pramana Budi Prasetyo Widyobroto Chusnul Hanim Chusnul Hidayat Damat, Damat Eko Suyanto Eko Suyanto Ellik Setyaningsih Endang S Rahayu Endang S. Rahayu Endang Sutariningsih Soetarto ENDANG SUTARININGSIH SOETARTO Endang Sutriswati Rahayu Endang Sutriswati Rahayu Endang Sutriswati Rahayu Endang Sutriswati Rahayu Endang Sutriswati Rahayu Fanny Arivia Davanti Haryadi Haryadi Haryadi Haryadi Heni Adhianata Hermina Nurdiawati Indrati, Retno Istna Nafi Azzahrani JAKA WIDADA Kharisma, Agung Dian Lies Mira Yusiati Lukitawesa Lukitawesa M. Margono Margono . Margono Margono Marta Tika Handayani Maulina Nurikasari Merkuria Karyantina Mohamad Soejono Mohamad Soejono Nanik Suhartatik Prima Interpares Puji Wulandari R. Rochmadi Rahayu, Novia Aristi Ria Millati Rini Yanti Rini Yanti, Rini Ristanto Utomo Ristiarini, Susana Rita Khairina Rochmadi - Rochmadi Rochmadi Sardjono Sardjono Sari Darmasiwi Sarto Shannora Yuliasari Siti Syamsiah Siti Syamsiah Siti Syamsiah Sri Rahardjo Sri Raharjo Sri Raharjo Sri Raharjo Stefani Amanda Harmani Suci Apsari Pebrianti Suci Wulandari Suci Wulandari Titiek Farianti Djaafar Tyas Utami Tyas Utami Umar Santosa Umar Santoso Wangi, Dyah Sekar A P Wulandari, Pudji Y. Marsono Yelmira Zalfiatri Yudi Pranoto Yunan K. Sya'di Yunan K. Sya’di, Yunan Zaenal Bachruddin Zulafa Noor Zuprizal (Zuprizal)