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All Journal JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Preventif Journal Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Prima Medika Sains Community Medicine and Education Journal Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Buletin Kedokteran dan Kesehatan Prima
Adek Amansyah
Sub-Department of Fertility Endocrinological Reproductives Department of Obstetrics and Gynecology
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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The Number of LH Receptor could Predict the Success of Oocyte Maturity in the Process of In Vitro Maturation Amansyah, Adek
Indonesian Journal of Obstetrics and Gynecology Volume. 1, No. 4, October 2013
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Objective: To evaluate the relationship between the number of LH receptor and the success of oocyte maturity in the process of in vitro maturation (IVM). Method: This experimental study was conducted in the Permata Hati Infertility Clinical Laboratory, Dr. Sardjito General Hospital, Yogyakarta, with the samples of 300 oocytes obtained through collecting immature cow’s oocytes from the abattoir and grouped the oocytes into 3 (three) groups based on the pattern of oocyte cumulus cells on the vesicle germinal stage 2 - 8 mm with three layers of cumulus cell. The sample of the cumulus cells from these three groups were taken and the LH receptor examination was done with immunohistochemistry. After that, the IVM process was performed to the three groups and its development for 24 hours was evaluated. Its maturation quality was evaluated with the emergence of the first polar body (1PB) and compared to the other groups and related to the number of LH receptor in the three groups. Result: The result of this study indicated that the oocyte cumulus cells showed a difference of function during IVM process. The maturity rate in this study showed that the number of LH receptor was related to the morphological pattern of oocyte cumulus cells with oocyte maturity. The maturity of the cumulus cells which 100% covered the oocyte was higher than that of the cumulus cells which > 50% and < 30% covered the oocytes, namely, 74% compared to 60% and 12%. The result of this study also showed that the average number of LH receptors in the three groups (A, B, and C) was 183.4, 78.8, and 24.0 respectively. A significant difference was found in the three groups (p < 0.0001). When related to IVM maturity, this difference showed that the bigger number of oocyte cumulus cells influenced the oocyte maturity. Conclusion: The number of LH receptor can be used as a prediction to determine the success of oocyte maturation in the process of in vitro maturation. [Indones J Obstet Gynecol 2013; 1-4:183-7] Keywords: IVM, LH receptor, oocyte cumulus cell
The Number of LH Receptor could Predict the Success of Oocyte Maturity in the Process of In Vitro Maturation Amansyah, Adek
Indonesian Journal of Obstetrics and Gynecology Volume. 1, No. 4, October 2013
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v1i4.363

Abstract

Objective: To evaluate the relationship between the number of LH receptor and the success of oocyte maturity in the process of in vitro maturation (IVM). Method: This experimental study was conducted in the Permata Hati Infertility Clinical Laboratory, Dr. Sardjito General Hospital, Yogyakarta, with the samples of 300 oocytes obtained through collecting immature cow’s oocytes from the abattoir and grouped the oocytes into 3 (three) groups based on the pattern of oocyte cumulus cells on the vesicle germinal stage 2 - 8 mm with three layers of cumulus cell. The sample of the cumulus cells from these three groups were taken and the LH receptor examination was done with immunohistochemistry. After that, the IVM process was performed to the three groups and its development for 24 hours was evaluated. Its maturation quality was evaluated with the emergence of the first polar body (1PB) and compared to the other groups and related to the number of LH receptor in the three groups. Result: The result of this study indicated that the oocyte cumulus cells showed a difference of function during IVM process. The maturity rate in this study showed that the number of LH receptor was related to the morphological pattern of oocyte cumulus cells with oocyte maturity. The maturity of the cumulus cells which 100% covered the oocyte was higher than that of the cumulus cells which > 50% and < 30% covered the oocytes, namely, 74% compared to 60% and 12%. The result of this study also showed that the average number of LH receptors in the three groups (A, B, and C) was 183.4, 78.8, and 24.0 respectively. A significant difference was found in the three groups (p < 0.0001). When related to IVM maturity, this difference showed that the bigger number of oocyte cumulus cells influenced the oocyte maturity. Conclusion: The number of LH receptor can be used as a prediction to determine the success of oocyte maturation in the process of in vitro maturation. [Indones J Obstet Gynecol 2013; 1-4:183-7] Keywords: IVM, LH receptor, oocyte cumulus cell
FAKTOR-FAKTOR YANG MEMPENGARUHI KEBERHASILAN TERAPI DIABETES MELLITUS GESTASIONAL DI PUSKESMAS PADANG BULAN SELAYANG II TAHUN 2020 Hasibuan, Ahmad Hasanuddin; Amansyah, Adek; Lina, Juliana
(Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Vol 6, No 1 (2021): JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/jimkesmas.v6i1.16225

Abstract

AbstrakPenelitian ini Untuk mencari Faktor yang dapat ..mempengaruhi..keberhasilan..terapi pada..penderita..diabetes..mellitus. gestasional.Penelitian ini adalah penelitian deskriftif, yang mengambilresponden di Puskesmas Padang Bulan Selayang II pada bulan April – September 2020. Populasi adalah ibu hamilyang menderita diabetes mellitus gestasional sebanyak 25 Responden, analisis yang digunakan adalah analisisunuvariat dan bivariat. Dari uji..chi-square.. tingkat signifikansi sebesar 0,003 dimana signifikansi < 0,05.Menunjukkan ada pengaruh..perilaku terhadap Keberhasilan Terapi Diabetes Mellitus Gestasional.Hasil..menunjukkan..tingkat..signifikansi..sebesar 0,013 dimana nilai sig persepsi kerentanan < 0,05.’Menunjukkan..ada..pengaruh persepsi..kerentanan dengan Keberhasilan Terapi Diabetes Mellitus Gestasional...Tingkat..signifikansi sebesar 0,002,,untuk..persepsi,,keseriusan < =0,05. Ada pengaruh persepsi keseriusandengan Keberhasilan Terapi Diabetes Mellitus Gestasional...uji chi-square.. tingkat signifikansisebesar..0,000.signifikansi dorongan < 0,05. Menunjukkan..ada pengaruh bermakna dari dorongan denganKeberhasilan Terapi Diabetes Mellitus Gestasional. Hasil uji chi-square menunjukkan tingkat signifikansi 0,024signifikansi ɑ <..0,05. Memiliki pengaruh..antara..pengetahuan dengan..Keberhasilan Terapi Diabetes MellitusGestasional. Prilaku, persepsi kerentanan, persepsi keseriusan, dorongan dan pengetahuan memiliki pengaruhterhadap keberhasilan terapi diabetes mellitus gestasional.Kata Kunci : Prilaku, Persepsi kerentanan, persepsi keseriusan, dorongan, pengetahuan, diabetes mellitusgestasional.
DAYA HAMBAT EKSTRAK ETANOL BIJI KETUMBAR (CORIANDRUM SATIVUM L.) TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS Pratama, Irza Haicha; Amansyah, Adek; Zai, Zulvan; Sitanggang, Anggi K T
Preventif Journal Vol 6, No 1 (2021): Preventif Journal
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/epj.v6i1.23230

Abstract

AbstrakBiji ketumbar (Coriandrum Sativum L) adalah tanaman yang diperkenalkan dari daerah Mediterranean, dan Timur Tengah, di Negara Indonesia biji ketumbar pada umumnya di gunakan sebagai bumbu masakan. Biji ketumbar juga banyak dimanfaatkan untuk penyakit diabetes, anti-inflamasi, antioxidant, anti fungal, antibiotik baik bakteri maupun jamur. Pada penelitian ini ekstraksi biji ketumbar dilakukan dengan cara maserasi menggunakan pelarut etanol 70%. Ada beberapa tingkatan konsentrasi yang digunakan yaitu 50%, 75%, 100%, dengan control positif gentamicin 10 mcg dan control negatif Aquadest steril. Teknik pengujian dilakukan sebanyak 5 kali pengulangan dengan metode difusi cakram. Pada hasil skrining fitokimia ekstrak biji ketumbar didapatkan adanya senyawa flavonoid, saponin, tannin, alkaloid, fenolik, glikosida, dan triterpenoid. Dari hasil pengujian ekstrak biji ketumbar dengan konsentrasi 100% didapatkan hasil bahwa ekstrak biji ketumbar sangat berpotensial menghambat pertumbuhan bakteri Staphylococcus aureus dengan hasil rata-rata diameter 8,20 mm dengan daya hambat tergolong besar
CORRELATION BETWEEN BLOOD GLUCOSE LEVEL AND BODY MASS INDEX AMONG PREGNANT WOMEN WITH EXCESS BODY WEIGHT Surbakti, Betharia Lorenza Br; Amansyah, Adek; Lina, Juliana
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 7 No. 1 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (946.987 KB) | DOI: 10.22487/htj.v7i1.136

Abstract

Amount of 207 million (83.6%) women who give birth simultaneously are diagnosed with GDM in 2019. Prevalence of GDM reaches 1.9-3.6% in Indonesia. Amount of 40-60% of GDM will suffer from postpartum Non-Dependent Insulin Diabetes Mellitus. Hence, this study was aimed to look for the distribution of blood sugar levels among pregnant women who are overweight. This research was performed among pregnant women in the Obstetrics and Gynecology polyclinic of Royal Prima Medan Hospital against 41 people as the sample with a cross-sectional model (Longitudinal study). The aspects evaluated in this study were age, gestational age, BMI, occupation, income, recent education, history of gestational DM, Macrosomia, and obstetrics. All research data was analyzed with SPSS 25 by spearman correlation. The trend of random et blood sugar levels of pregnant women as respondents was 100 mg/dL, while the BMI tendency was 31.72 kg/m2. Based on these parameters, they had a correlation with each other among overweight pregnant women (Value P = 0.007) that was moderate (R = 0.417). Blood sugar levels were moderate correlated with BMI among pregnant women with excess body weight.
DAYA HAMBAT EKSTRAK ETANOL BIJI KETUMBAR (CORIANDRUM SATIVUM L.) TERHADAP PERTUMBUHAN BAKTERI PSEUDOMONAS AERUGINOSA Sitanggang, Anggi Kristina Theresia; Zai, Zulvan Jaya Putra; Pratama, Irza Haicha; Amansyah, Adek
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 7 No. 3 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (885.697 KB) | DOI: 10.22487/htj.v7i3.470

Abstract

Biji ketumbar (Coriandrum Sativum L) memupakan tanaman yang berasal dari Mediterranean, dan Timur Tengah, masyarakat Indonesia umumnya di manfaat kan sebagai bumbu masak. Biji ketumbar sering juga digunakan untuk penyakit diabetes, diuretic, hypolipidemia, anti- fungal, antibiotic baik jamur maupun bakteri, antioxidan, dan anti-inflamasi. Ekstraksi biji ketumbar dilakukan dengan cara maserasi menggunakan pelarut etanol 70%. Konsentrasi yag digunakan 50%, 75%, dan 100%, dengan control positif gentamisin 10mcg (OXOID) serta control negative (aquadest steril). Pengujian dilakukan dua kali pengulangan dengan metode difusi cakram. Skrining fitokimia ekstrak biji ketumbar menunjukan adanya senyawa alkaloid, saponin, tannin, flavonoid, fenolik, Triterpenoid, dan glikosida. Hasil pengujian menunjukan bahwa ekstrak biji ketumbar mampu menghambat petumbuhan bakteri pseudomonas aeruginosa. Ekstrak biji ketumbar yang mampu menghambat pertumbuhan bakteri pseudomonas aeruginosa adalah konsentrasi 100% dan rata-rata diameter 8,20 mm dengan daya hambat tergolong besar.
Detection of glibenclamide adulteration in herbal remedies for diabetes mellitus using TLC Budi, Andre; Razoki, Razoki; Harahap, Halimah; Amansyah, Adek; Edlin, Edlin; Tarigan, Susiani
Buletin Kedokteran & Kesehatan Prima Vol. 3 No. 1 (2024): March
Publisher : Fakultas Kedokteran, Kedokteran Gigi, dan Ilmu Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/bkkp.v3i1.5287

Abstract

The widespread use of jamu, a traditional Indonesian herbal medicine, has been accompanied by a surge in adulteration with synthetic drugs to enhance efficacy and sales. This study aimed to detect the presence of glibenclamide, a common adulterant, in antidiabetic jamu product using TLC. This study employed three herbal medicine samples marketed for lowering blood glucose levels, with glibenclamide BPFI serving as a reference standard. Thin-layer chromatography, employing a mobile phase consisting of chloroform, cyclohexane, ethanol, and glacial acetic acid (9:9:1:1), was utilized for the analysis. Thin-layer chromatography (TLC) analysis revealed a standard Rf value of 0.4 for glibenclamide, producing a distinct blue-violet spot when visualized with 10% sulfuric acid. The results indicated that the tested herbal medicine samples did not contain the pharmaceutical substance glibenclamide. This conclusion was drawn from the absence of a corresponding Rf value for glibenclamide in the herbal samples when compared to the reference standard.
Hubungan jumlah paritas terhadap usia menopause Grasiah, Joster; Amansyah, Adek; Pratama, Irza Haicha; Djohan, Djohan
Jurnal Prima Medika Sains Vol. 4 No. 1 (2022): Juni
Publisher : Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jpms.v4i1.2260

Abstract

Semakin meningkatnya jumlah wanita berusia 59 tahun di Indonesia, menjadi penting untuk mecegah menopause pada usia yang terlalu dini. Jumlah paritas merupakan salah satu faktor yang penting untuk mencegah menopause lebih cepat. Oleh karena itu penelitian ini bertujuan untuk mengetahui hubungan antara paritas terhadap menopause pada pasien di Rumah Sakit Umum Royal Prima. Penelitian ini merupakan penelitian cross-sectional pada 30 pasien menopause di Rumah Sakit Umum Royal Prima yang dipilih dengan simple random sampling. Analisa data yang dilakukan untuk menilai pengaruh jumlah paritas terhadap usia menopause adalah uji Kruskal-Wallis dan korelasi spearman rho. Hasil penelitian menunjukkan bahwa rata-rata pasien menopause berada pada usia 67.10 tahun dengan kecenderungan mengalamai menopause pada usia 54 tahun. Terdapat perbedaan yang signifikan terhadap usia menopause pada masing-masing kelompok paritas (p= 0,023). Selain itu, hasil analisa statistik lainnya juga menunjukkan bahwa terdapat korelasi yang bersifat positif antara jumlah paritas terhadap usia menopause (p= 0.05; koefisien korelasi: 0.874). Oleh karena itu, jumlah paritas secara signifikan mempengaruhi usia menopause, dimana pasien dengan jumlah paritas yang banyak cenderung mengalami menopause yang lebih lama.
The Role of Physical Activity in Menopausal Women Amansyah, Adek; Naurah Al’Ariqoh; Meldawati
Community Medicine and Education Journal Vol. 5 No. 1 (2024): Community Medicine and Education Journal
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/cmej.v5i1.441

Abstract

Menopause is the permanent cessation of the menstrual cycle due to decreased ovarian function, resulting in a decrease in follicles. A person is said to be experiencing menopause if they do not experience menstruation for 12 months. Symptoms that occur during menopause include somatic, psychological, and urogenital symptoms. Exercise has many benefits for body health. Exercise, such as regular physical activity, is considered to reduce complaints during menopause. This study aims to identify the relationship between various physical activities and menopausal symptoms in women aged 50-60 years in the working area of the Ujunggading Health Center. This research uses a correlation research design with a cross-sectional approach model with a sample size of 75 respondents using the nonprobability sampling method, namely using purposive sampling, data collection using GPAQ and MRS questionnaires, and data analysis using correlation tests. The results of the research from 75 respondents showed that the frequency distribution of the characteristics of respondents with the highest occupation was as a housewife, namely 23 people (30.7%), the highest education was junior high school, namely 20 people (26.7%), and the highest duration of menopause was 5-7 years, namely 36 people (48.0%). Shows that many respondents have physical activity in the moderate category 54 (72%), and many respondents experience menopausal symptoms in the moderate category 37 (49.3%). There is a relationship between physical activity and menopausal symptoms with a p-value = 0.000 (p<0.05). There is a relationship between physical activity and menopausal symptoms where the higher the physical activity carried out, the less menopausal symptoms are felt, menopausal symptoms can be reduced by routinely doing activities and exercising for 15 to 60 minutes every day.
Co-Authors Budi, Andre Djohan Djohan Edlin, Edlin Grasiah, Joster Harahap, Halimah Hasibuan, Ahmad Hasanuddin Lina, Juliana Meldawati Naurah Al’Ariqoh Pratama, Irza Haicha Razoki, Razoki Sitanggang, Anggi K T Sitanggang, Anggi Kristina Theresia Surbakti, Betharia Lorenza Br Tarigan, Susiani Zai, Zulvan Zai, Zulvan Jaya Putra