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All Journal Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture
R.I. Arifiantini
Faculty of Veterinary Medicine, Bogor Agricultural University, Darmaga Campus, Bogor 16680
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Comparison of four diluents for the retriever dogs semen preservation Wicaksono, A.; Arifiantini, R.I.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.22 KB) | DOI: 10.14334/jitv.v14i1.363

Abstract

The quality of chilled semen depends on the composition of diluent. The choice of the buffer, anti-cold shock and nutrition sources can be the first decision in order to choose appropriate diluents. Nowadays a lot of diluent are used for canine semen preservation such as Tris buffer and Citrate buffer. This study was aimed to observe the differences of diluent for preserving Retriever dog spermatozoa. The semen sample collected from four Retriever dogs with three times repetition. The semen was evaluated macro-and microscopically. The semen with >70% sperm motility was divided into four tubes and diluted with diluter 1 (P1), diluter: P2, P3 and P4 (modified P3). The diluted semen was divided into two tubes and each sample was stored at room and 50C temperature. The viability of chilled semen was observed every 3 hours at room temperature and 12 hours at 50C. The result showed that P2 keep the sperm viability better than the other diluents. On 50C at 24 hours storage P2 showed the highest motile and live sperm percentage (46.25 ± 0.22%; 57.11 ± 0.25%). In room temperature at 6 hours P2 showed the highest motile and live sperm percentage (40.94 ± 0.20%; 52.65 ± 0.23%). It is concluded that P2 can keep the sperm viability by 84 hours of 50C and 21 hours at room temperature. Key Words: Diluents, Dog Sperm, Retriever
MOTILITY AND VIABILITY OF FRIESIAN HOLSTEIN SPERMATOZOA IN THREE DIFFERENT EXTENDER STORED AT 5oC Arifiantini, R.I.; Purwantara, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 4 (2010): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.4.222-226

Abstract

The aims of this study was to compare Tris egg yolk and Citrate egg yolk extender andsupplementation of fructose on citrate egg yolk on the quality of Friesian Holstein (FH) bull semenstored at 5 oC. Semen was collected from 5 FH bulls using an artificial vagina. The semen wereevaluated macroscopic and microscopically. The semen divided into three tubes and extended with Trisegg yolk (TEY), Citrate egg yolk (CEY) or Citrate fructose egg yolk (CFEY). Extended semen wasstored at 5 oC and evaluate daily for sperm motility and viability. There was no significant differences(P>0.05) on the sperm viability among three extender, for every time observation during 144 hours ofstorage. This similar finding found on the sperm motility in all extender for 48 hours of storage. Thesperm motility in TEY demonstrated significantly greater (P<0.05) than in CFEY and CEY extender at72 to 120 hours storage. In the end of storage, sperm motility in TEY (35.2 ± 4.1%) and CFEY (33.5 ±2.71%) extender statistically indicated no significant different, and both were greater than CEY. Inconclusion, CFEY support the sperm motility as good as TEY of FH bull.
SPERM MORPHOLOGICAL ASSESSMENTS OF FRIESIAN HOLSTEIN BULL SEMEN COLLECTED FROM THREE ARTIFICIAL INSEMINATION CENTERS IN INDONESIA Purwantara, B.; Arifiantini, R.I.; Riyadhi, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 2 (2010): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.2.90-94

Abstract

Morphologically abnormal sperm in semen has been associated with the sub fertility and sterilityfor many years. This study assessed the sperm morphology of Friesian Holstein bull semen which wascollected from three Artificial Insemination centers in Indonesia. Total of 22 bulls were used in thisstudy; an ejaculate from each bull was examined. Three to four glass slides were prepared for each bullsample; a drop of semen was placed on each glass slide, smeared, and air-dried. The smeared sampleswere stained with carbolfuchsin-eosin (Williams stain). Morphological abnormality types were recordedfrom total of 500 spermatozoa. Results demonstrated that all 22 bulls had low sperm abnormality(<10%). Pearshaped was the most frequently type of sperm abnormality found in the samples(0.81±0.93%); while detached head was the lowest (0.01±0.04%).
EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION Arifiantini, R.I.; Purwantara, B.; Yusuf, T.I.; Sajuthi, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 1 (2010): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.1.68-74

Abstract

s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos(DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethyleneglycol-glycerol (EG+Gly) or dimethilformamide (DMF). Each semen sample was evaluated and dividedequally into six tubes; semen in the three tubes was diluted 1:1 with (SM), while in the remaining tubesthe semen was diluted 1:1 by DV. After being diluted, all tubes were centrifuged at 1006xg for 10minutes. The supernatan discarded, the pellet was rediluted by SM trehalosa or DV trehalose, and addedby G, EG+Gly, or DMF to reach the final sperm concentration of 200x106/ml. The extended semen wasindividually packed in 0.3 ml minitube, equilibrated at 4oC for 2 hours, frozen in liquid nitrogen vaporfor 10 minutes, and then was stored in liquid nitrogen container at -196 oC. After 24 hours, the semenwas thawed at 37 oC for 30 second. There were no significantly different (p>0.05) on the percentages ofmotile and viable sperm in SMT (21.7% and 43.4%, respectively) compared with those extended withDV T extender (26.9% and 50.8%, respectively). DMF demonstrated better results as CPA compared tothe others; and DVTDMF combination had the best protection during cryopreservation in this study.
THE HYPO-OSMOTIC SWELLING TEST IN FRESH GARUT RAM SPERMATOZOA Nalley, W.M.M.; Arifiantini, R.I.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 4 (2013): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.4.212-216

Abstract

The study was aimed to determine the optimal time to evaluate the maximum numbers of swollensperm after expose to hypo-osmotic solution for testing membrane integrity in fresh garut ram semen. Atotal of 24 ejaculate samples from eight sexually mature garut ram were collected using artificial vagina.After macroscopic evaluation, 10 μL undiluted semen was gently mixed in each of the 2 mL hypoosmoticsolution and incubated at 37oC for 1 hour. Two hundred of coil and non-coiled sperm wereobserved from five different fields every 15 minutes. Results demonstrated that the maximumpercentage of coiling sperm positive to HOS test was at 30 minutes and no differences among 8 garutrams used.
Co-Authors A. Wicaksono B. Purwantara D. Sajuthi M. Riyadhi T.I. Yusuf Wilmientje Marlene Mesang Nalley