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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Veteriner Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production ARSHI Veterinary Letters LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmu Peternakan Terapan Jurnal Nukleus Peternakan

Wilmientje Marlene Mesang Nalley

Fakultas Peternakan Universitas Nusa Cendana Kupang

Author-ID : 315961

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Veterinary

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Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature Mesang-Nalley, W. Marlene; Handarini, R; Purwantara, B
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

The successful Â sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in Â Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydratesÂ in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots.Â Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 Â± 0.63 ml, pH 7.03Â±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 Â± 7.4%. The average of sperm concentration was 842.35 Â± 258.14x106 ml-1, the viable sperm was 78.11 Â± 3.61%, the sperm abnormality was 7.31 Â± 2.98%. The percentages of sperm motility on TEYG (18.00 Â± 17.63%) and TEYS (21.83 Â± 15.92%) were higher compare to TEYF (4,00 Â± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 Â± 20.06) and TEYS (24.00 Â± 22.59%) (P<0.05) were significantly higher compare to TEYF (4.00 Â± 0.00%).Â It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be usedÂ for artificial insemination after 12 hour storage. Key Words: Liquid Semen, Deer, Room Temperature, Carbohydrate

Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3â5oC Rizal, Muhammad; ., Herdis; Surachman, Maman; Mesang-Nalley, W. Marlene
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3â5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control). Supernatant of the second tube was removed (treatment B or without seminal plasma). Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C). Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3â5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) was evaluated every day during storage at 3â5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6%) was significantly (P<0.05) higher than that of: treatment B (31, 44.8 and 45.2%) and treatment A (11, 15.6 and 14.8%). In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3â5oC for three days, and it prevent semen from coagulation. Key Words: Seminal Plasma, Priangan Ram, Spermatozoa Viability, PE Buck

The Viability of Local Ram Semen in Tris Buffer With Three Different Egg Yolks Nalley, WMM; Arifiantini, RI
ANIMAL PRODUCTION Vol 13, No 1 (2011): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.782 KB)

Egg yolk consisted of lecithin and phospholipids are one of the most commonly used components that will protect spermatozoa against cold shock during cooling and freezing.Â The aim of this study was to evaluate the effect of different hen egg yolk on Tris extender on the freezability of local ram semen. Semen from six sexually matured local rams was collected weekly using artificial vagina. Collected semen was evaluated macroscopically and microscopically and extendedÂ using tris extender consisted of 20% (v/v) regularÂ egg yolk (TRCEY), native egg yolk (TNCEY), omega-3 hen egg yolk (TOEY) and 6% (v/v) glycerol. Those were packed in 0.25 ml straws, equilibrated at 5oC for 3 hours, frozen and stored in nitrogen tank for 24 hours, and thawed at 37oC for 30 second. The result of the experiment showed that there were no significant differences on the sperm motility and the number of living sperm. Percentage of plasma membrane intact in TOEY (60.3%) was significantly higher compared to TREY (56.9%) and TNEY (55.6%). In conclusion, the addition of omega 3 egg yolk in Tris extender protects plasma membrane better than the regular or native hen egg yolk. (Animal Production 13 (1):39-44 (2011)Â Key Words: ram semen, egg yolk, frozen semen

THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN Nalley, W.M.M.; Handarini, R.; Yusuf, T.L.; Purwantara, B.; Semiadi, G.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.91-96

The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY)diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer usingelectroejaculator. Immediately after collection the semen was evaluated macroscopic andmicroscopically. After initial evaluation, the semen was divided into three tubes and extended with Trisegg yolk with three different glycerol concentrations, which were 10% (TGEY10); 12% (TGEY12) and14% (TGEY14). The sperm motility, viability, acrosome intact and membrane intact were evaluated inraw semen, after equilibration and after thawing. The results showed that there were no differences(p>0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact inTGEY10 (52.50±5.89%) and TGEY14 (51.50±4.12 %) were higher (p<0.05) than in TGEY12(49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deersemen cryopreservation.

THE HYPO-OSMOTIC SWELLING TEST IN FRESH GARUT RAM SPERMATOZOA Nalley, W.M.M.; Arifiantini, R.I.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 4 (2013): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.4.212-216

The study was aimed to determine the optimal time to evaluate the maximum numbers of swollensperm after expose to hypo-osmotic solution for testing membrane integrity in fresh garut ram semen. Atotal of 24 ejaculate samples from eight sexually mature garut ram were collected using artificial vagina.After macroscopic evaluation, 10 μL undiluted semen was gently mixed in each of the 2 mL hypoosmoticsolution and incubated at 37oC for 1 hour. Two hundred of coil and non-coiled sperm wereobserved from five different fields every 15 minutes. Results demonstrated that the maximumpercentage of coiling sperm positive to HOS test was at 30 minutes and no differences among 8 garutrams used.

Deer Frozen Semen Quality in Tris Sucrose and Tris Glucose Extender with Different Glycerol Concentrations W M. M Nalley; R Handarini; R I Arifiantini; T L Yusuf; B Purwantara; G Semiadi
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

In order to improve Timor deer (Cervus timorensis) frozen semen quality, the influence of sugar and glycerol concentration on semen characteristics of sperm was investigated. The semen was collected from five sexually mature Timor deer using an electroejaculator. The semen was evaluated and divided into six equal tubes and diluted with Tris sucrose glycerol 10% (TSG10); Tris sucrose glycerol 12% (TSG12); Tris sucrose glycerol 14% (TSG14); Tris glucose glycerol 10% (TGG10); Tris glucose glycerol 12% (TGG12); and Tris glucose glycerol 14% (TGG14). The diluted semen was packed in 0.3 ml minitub straw, equilibrated at 5 oC for 4 hours and frozen on liquid nitrogen vapor for 10 minutes. The total of forward motility, viability, acrosome integrity and membrane integrity were assessed in fresh, after equilibration and after thawing. Results demonstrated that the percentage of sperm motility in TSG10was higher (P (63.93±7.23%). The sperm in TSG10 and TSG14 extender were superior in acrosome as well as in membrane integrity. It was concluded that Tris Sucrose with 10% glycerol protected Timor deer sperm better than other combinations.

Tampilan Estrus dan Angka Kebuntingan Sapi Bali Pascapemberian Ekstrak Pituitari Wilmientje Marlene Nalley; Petrus Kune; Thomas Mata Hine
Acta VETERINARIA Indonesiana Vol. 5 No. 2 (2017): Juli 2017
Publisher : IPB University

Tujuan penelitian ini adalah untuk menguji potensi ekstrak pituitari sebagai sumber hormon alami yang murah dan aplikatif untuk meningkatkan produktivitas induk sapi bali. Kegiatan penelitian diawali dengan koleksi kelenjar pituitari dari rumah potong hewan dan diawetkan menggunakan aseton konsentrasi dengan bertingkat. Kelenjar pituitari kemudian dilarutkan dengan aquabidest menggunakan mortar hingga larut, larutan kelenjar pituitari disentrifugasi, dan supernatan disimpan sebagai ekstrak pituitari (EP). Dua belas ekor sapi bali dibagi ke dalam 4 kelompok. Kelompok I dosis 0 ml (P0, kontrol), kelompok II 10 mg/kg berat badan (BB) (P1), Kelompok III 20 mg/kg BB (P2), dan kelompok IV 30 mg/kg BB (P3). Variabel penelitian adalah tampilan estrus, tingkat ovulasi, angka kebuntingan dan service per conception. Hasil penelitian menunjukkan pemberian 20 mg/kg BB EP pada induk sapi bali mampu memperpendek siklus estrus (P<0,05) yaitu 11,67 hari dibandingkan dengan kontrol 27 hari; meningkatkan angka kebuntingan hingga 100% vs 0%, dan menurunkan service per conception yaitu 1,00 vs 3,33. Tidak terdapat perbedaan dalam ovulatiton rate dalam intensitas estrus antar perlakuan (P>0,05). Kesimpulan penelitian ini adalah esktrak pituitari dapat meningkatkan produktivitas induksi sapi bali, dengan dosis terbaik yaitu 20 mg/kg BB.

PROFIL HORMON TESTOSTERON RUSA TIMOR (Cervus timorensis) JANTAN DALAM SATU SIKLUS RANGGAH (Testosterone Hormone Profile in One Antler Cycle of Timor Deer (Cervus timorensis) stag) R. Handarini; W. M. Nalley
Media Konservasi Vol 13 No 3 (2008): Media Konservasi
Publisher : Department of Forest Resources Conservation and Ecotourism - IPB University

The growth and replacement of stags antler on temperate species is physiologically linked to the seasonal rhythms of reproductive hormones. The objective of this research was to determine the pattern of testosterone hormone secretion in timor stags during one annual antler cycle which could be used to determine the active reproductive period of the stag. This research was conducted for 18 months in four adult timor stags (4 – 6 years of age). Blood samples were collected at three weeks intervals for analysis of testosterone hormone using the radioimmunoassay (RIA) method during one antler cycle (i.e. velvet, hard antler and casting stage). Results of this research showed significant difference (P

Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature W. Marlene Mesang-Nalley; R Handarini; B Purwantara
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The successful sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots. Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63%) and TEYS (21.83 ± 15.92%) were higher compare to TEYF (4,00 ± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06) and TEYS (24.00 ± 22.59%) (P<0.05) were significantly higher compare to TEYF (4.00 ± 0.00%). It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used for artificial insemination after 12 hour storage. Key Words: Liquid Semen, Deer, Room Temperature, Carbohydrate

Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC Muhammad Rizal; Herdis .; Maman Surachman; W. Marlene Mesang-Nalley
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control). Supernatant of the second tube was removed (treatment B or without seminal plasma). Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C). Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) was evaluated every day during storage at 3–5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6%) was significantly (P<0.05) higher than that of: treatment B (31, 44.8 and 45.2%) and treatment A (11, 15.6 and 14.8%). In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3–5oC for three days, and it prevent semen from coagulation. Key Words: Seminal Plasma, Priangan Ram, Spermatozoa Viability, PE Buck

Co-Authors A. B. Lawa A. Kaka A. N. Banamtuan Agus Saputra Aleksander Kaka Alfred Nubatonis Aloysius Marawali Anak Agung Gede Sugianthara Arnoldianus Harbin B Purwantara B Purwantara B. Purwantara Bambang Purwantara Biqshierz V.W Koelima Burhanuddin * Burhanuddin burhanuddin Demianus Ferdinand Souhoka Fian K. Djita Franky M S Telupere G Semiadi G. Semiadi Gaina, Cynthia Dewi GONO SEMIADI Henderiana Belli Hendriana L.L. Belli Herdis . Heri Armadianto Heri Armadianto I Made Adi Sudarma Imanuel Benu J A Tamoes Jernih Blegur Johny N. Kihe kirenius uly Kirenius Uly Kirenius Uly Maman Surachman Maman Surachman Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria F. T. Leyn Maria G. Mega Maria M. Bria Mariana Nenobais Maritje Aleonor Hilakore Maxs Urias Ebenhaizar Sanam, Maxs Urias Melianus Fafo Michel Johan Matatula MUHAMMAD RIZAL N.G.A Mulyantini S.S Petrus Kune Petrus Kune Petrus Kune R Handarini R I Arifiantini R. Handarini R. Handarini R. Iis Arifiantini R.I. Arifiantini Reni Ratni Dapawole RI Arifiantini, RI Ristika Handarini Satria J.S. Rihileo Selsiana Dongkot Sofia Marlize Soleman Manaze Weiri Sinda T L Yusuf T.L. Yusuf Thomas Hine Thomas Mata Hine Tree May Thiessen Outang Tuty Laswardi Yusuf Twen O. Dami Dato Ulrikus R. Lole Wirona Adu Yohanis Umbu Daku Waluwanja

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