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PREPARASI DAN KARAKTERISASI KITIN YANG TERKANDUNG DALAM EKSOSKELETON KUMBANG TANDUK RHINOCEROS BEETLE (XYLOTRUPES GIDEON L) DAN KUTU BERAS (SITOPHILUS ORYZAE L) Komariah, Komariah; Astuti, Luki
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.139 KB)

Abstract

ABSTRAK   Penelitian ini bertujuan mengkarakterisasi kitin hasil preparasi dari eksoskeleton kumbang tanduk Rhinoceros Beetle (Xylotrupes gideon L) dan kutu beras (Sitophilus oryzae). Penelitian terbagi menjadi penelitian pendahuluan yaitu preparasi dan uji proksimat, dan penelitian utama yang terdiri dari proses pembuatan dan karakterisasi kitin. Proses pembuatan kitin diawali dengan uji demineralisasi (HCL 1 N, 90°C), deproteinisasi (NaOH 3 N, 90°C), dan dekalorisasi (NaOCl 4%, suhu kamar). Karakterisasi meliputi tekstur, rendemen, kelarutan, kadar air, kadar abu, kadar nitrogen, kadar mineral dan derajat deasetilasi. Kadar abu dan kadar air ditentukan dengan gravimetri, kadar protein dengan kjedahl. Uji Kelarutan dengan asam asetat 2%, kadar mineral dengan spektrofotometer, sedangkan derajat deasetilasi dianalisis dengan menggunakan First Derivative Ultra Violet Spektrofotometry. Berdasarkan hasil perhitungan memperlihatkan karakteristik kitin dari eksoskeleton Rhinoceros Beetle (Xylotrupes gideon) menghasilkan kadar abu 0,98%, kadar air 8,37%, kadar nitrogen 3,02%, derajat deasetilasi 44,84%. Karakteristik kitin dari eksoskeleton Sitophilus oryzae menghasilkan kadar abu 2,00%, kadar air 8,00%, kadar nitrogen 3,57%, derajat deasetilasi 28,60 %. Kitin dari kedua sempel tidak larut dalam asam asetat 2%, berwarna putih dan tidak berbau. Hasil yang diperoleh sesuai dengan kriteria mutu kitin.   Kata Kunci: kitin, kadar air, kadar abu, kadar nitrogen, kelarutan dan derajat deasetilasi.
Comparison between probiotic lozenges and drinks towards periodontal status improvement of orthodontic patients Natasia Melita Kohar; Victor Emmanuel; Luki Astuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 3 (2015): September 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.095 KB) | DOI: 10.20473/j.djmkg.v48.i3.p126-129

Abstract

Background: Fixed orthodontic appliances may interfere with daily oral hygiene procedure, causing more abundant plaque accumulation, therefore increasing the risk of periodontal disease. Probiotic methods represent a breakthrough approach in maintaining oral health and preventing periodontal disease. Purpose: The aim of this study was to compare the effect between probiotic lozenges containing Lactobacillus reuteri and probiotic drinks containing Lactobacillus casei strain Shirota towards periodontal status of orthodontic patients. Method: Fixed orthodontic patients (n=30) from Faculty of Dentistry, Trisakti University Dental Hospital were included in this clinical trial. Periodontal status consisting of Plaque Index (PlI), Interdental Hygiene Index (HYG), and Papillary Bleeding Index (PBI) were then recorded from each patient. All patients received the phase one of periodontal treatment, as well as plaque control instruction. The subjects (n=10/gp) were randomly assigned to one of three groups; control group; probiotic lozenges group (Biogaia®); and probiotic drinks group (Yakult®). For 14 days, the probiotic groups were instructed to use the probiotic. Periodontal index improvement (PlI, HYG, and PBI) was found in all groups after 14 days research periode. These indices were then analyzed using Kruskal-Wallis analysis test. Result: It was found that L. reuteri and L. casei strain Shirota may improve periodontal status in fixed orthodontic patients. The best results were obtained from probiotic lozenges group. However, the results were not statistically significant (p>0.05). Conclusion: It was concluded that probiotics consumption containing L. reuteri and L. casei strain Shirota may slightly improve periodontal status in fixed orthodontic patients.
PREPARASI DAN KARAKTERISASI KITIN YANG TERKANDUNG DALAM EKSOSKELETON KUMBANG TANDUK RHINOCEROS BEETLE (Xylotrupes gideon L) DAN KUTU BERAS (Sitophilus oryzae L) Komariah Komariah; Luki Astuti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 9, No 1 (2012): Prosiding Seminar Nasional IX Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK   Penelitian ini bertujuan mengkarakterisasi kitin hasil preparasi dari eksoskeleton kumbang tanduk Rhinoceros Beetle (Xylotrupes gideon L) dan kutu beras (Sitophilus oryzae). Penelitian terbagi menjadi penelitian pendahuluan yaitu preparasi dan uji proksimat, dan penelitian utama yang terdiri dari proses pembuatan dan karakterisasi kitin. Proses pembuatan kitin diawali dengan uji demineralisasi (HCL 1 N, 90°C), deproteinisasi (NaOH 3 N, 90°C), dan dekalorisasi (NaOCl 4%, suhu kamar). Karakterisasi meliputi tekstur, rendemen, kelarutan, kadar air, kadar abu, kadar nitrogen, kadar mineral dan derajat deasetilasi. Kadar abu dan kadar air ditentukan dengan gravimetri, kadar protein dengan kjedahl. Uji Kelarutan dengan asam asetat 2%, kadar mineral dengan spektrofotometer, sedangkan derajat deasetilasi dianalisis dengan menggunakan First Derivative Ultra Violet Spektrofotometry. Berdasarkan hasil perhitungan memperlihatkan karakteristik kitin dari eksoskeleton Rhinoceros Beetle (Xylotrupes gideon) menghasilkan kadar abu 0,98%, kadar air 8,37%, kadar nitrogen 3,02%, derajat deasetilasi 44,84%. Karakteristik kitin dari eksoskeleton Sitophilus oryzae menghasilkan kadar abu 2,00%, kadar air 8,00%, kadar nitrogen 3,57%, derajat deasetilasi 28,60 %. Kitin dari kedua sempel tidak larut dalam asam asetat 2%, berwarna putih dan tidak berbau. Hasil yang diperoleh sesuai dengan kriteria mutu kitin.   Kata Kunci: kitin, kadar air, kadar abu, kadar nitrogen, kelarutan dan derajat deasetilasi.
Karakteristik Sosiodemografi Penderita Diabetes Melitus Yang Mengalami Kelainan Periodontal: A Scoping Review Annisa Nur Diana; Luki Astuti; Lia Hapsari Andayani
Jurnal Kedokteran Gigi Terpadu Vol. 3 No. 2 (2021): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.385 KB) | DOI: 10.25105/jkgt.v3i2.12662

Abstract

Background: Diabetes mellitus and periodontal disease are a multifactorial diseases which have a bidirectional relationship. Diabetes mellitus can increase the risk of periodontal diseases while periodontal disease can interfere the glycemic control in diabetic patients by increasing insulin resistance. The epidemiology of both diseases are known to be related by the individuals characteristics of sociodemographic such as gender, age, educational level, socioeconomic and lifestyle. Objective: The aim of this study was to see the sociodemographic characteristics of diabetes mellitus patients with periodontal disease based on gender, age, educational level, socioeconomy, and lifestyle. Method: This type of research is a scoping review using Pubmed and Google Scholar as an electronic databases. Journals with observational study design, such as cross-sectional, case-control, and cohort in English and being published since 2019 were included in the inclusion criteria. Results: 6 journals were obtained according to the inclusion criteria. The increased risk of periodontal disease have a variation in gender, age, and socioeconomic variables. Meanwhile, the variables of educational level and smoking lifestyle showed that there was no variation in the increased risk of periodontal disease. Conclusion: Characteristic of sociodemographic in diabetes mellitus patients suffering periodontal disease have a variations in each variables.
Suplementasi asam folat untuk mengurangi pembesaran gingiva akibat terapi fenitoin: a scoping review Alma Rizkita Nabila; Luki Astuti; Lia Hapsari Andayani
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.292 KB) | DOI: 10.25105/jkgt.v4i1.14478

Abstract

Background: Epilepsy is a neurological condition that around 70 million people worldwide have. Phenytoin is the most common anti-epileptic therapy for controlling epilepsy and preventing its recurrence. The side effect of phenytoin therapy includes gingival overgrowth which often causes psychological and aesthetical problems, interferes with normal mastication, and worsen the gingival inflammation. There are surgical and non-surgical options to treat phenytoin-induced gingival overgrowth, one of the non-surgical options to treat phenytoin-induced gingival overgrowth. One of the non-surgical treatment for phenytoin-induced gingival overgrowth is folic acid supplementation, which has been studied to prevent the adverse side effects of phenytoin therapy despite the variative results. Objective: To discover the current theory depiction about the effect of folic acid supplementation on gingival overgrowth due to phenytoin therapy. Method: The type of the research is descriptive observational research and method of scoping review. Journals obtained using PCC (population, concept, and context) with population of patients on phenytoin therapy, using concept of preventing phenytoin-induced gingival overgrowth using agent supplementation, and context of folic acid. The inclusion and exclusion criteria filtered by boolean search on two databases, PubMed and Google Scholar from 1987 to 2020. Results: A total of 5 journals found and all of them are included in a table with the results of the studies discussing about folic acid supplementation in phenytoin-induced gingival overgrowth. Conclusion: Folic acid supplementation was found to delay the onset, reduce the incidence and severity as well as the risk of recurrence after gingivectomy on phenytoin-induced gingival overgrowth.
PEMBERDAYAAN MASYARAKAT DALAM MENINGKATKAN KESEHATAN DAN MENCEGAH INFEKSI RONGGA MULUT SELAMA PANDEMI COVID-19 Yessy Ariesanti; Ferry Sandra; Luki Astuti; M Novo P Lubis; Caesary Cloudya P; Irvan Septrian S P R
Jurnal Abdi Masyarakat Indonesia (JAMIN) Vol 3 No 1 (2021): JURNAL ABDI MASYARAKAT INDONESIA (JAMIN)
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1072.143 KB) | DOI: 10.25105/jamin.v3i1.8205

Abstract

Pandemi Covid-19 berdampak pada pelayanan kesehatan gigi dan mulut. Kunjungan ke dokter gigi selama pandemi disarankan untuk kasus-kasus darurat seperti infeksi. Edukasi pada masyarakat diperlukan untuk menjaga kesehatan dan mencegah infeksi pada rongga mulut. Bahan alam seperti larutan air garam memiliki efek antiseptik dan rebusan daun sirih memiliki efek bakterisid, antioksidan dan antijamur sehingga dapat digunakan untuk menjaga kesehatan dan mengatasi infeksi ringan rongga mulut. PkM ini bertujuan untuk pemberdayaan masyarakat dengan memberikan pendidikan dan latihan menjaga kesehatan dan mencegah infeksi rongga mulut dengan cara menyikat gigi yang benar, penggunaan obat kumur larutan air garam dan rebusan daun sirih. Kegiatan diikuti oleh 31 anggota komunitas Pemuda Pancasila sebagai kader di Kabupaten Bogor yang terdiri dari pelajar SMA/sederajat, D4 dan S1 dengan pekerjaan wirausaha, wiraswasta dan karyawan. Kegiatan dilakukan secara daring (Zoom), diawali pre-test, pemaparan materi pendidikan dan latihan, kemudian diakhiri post-test. Hasil pre-test dan post-test menunjukan peningkatan pengetahuan peserta dengan rata-rata nilai 56 menjadi 81. Usia dewasa (74%) dan remaja (26%), pendidikan terakhir SMA/sederajat (74%), D4 (3%), S1 (23%), belum bekerja (55%), wirausaha (6%), wiraswasta (29%) dan karyawan (10%). Kesimpulan: Pendidikan dan latihan untuk menjaga kesehatan gigi dan mulut dapat diterapkan oleh komunitas sebagai kader masyarakat Kabupaten Bogor.
Penatalaksanaan altered passive eruption dengan crown lenghtening (Laporan Kasus) Luki Astuti
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 2 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i2.15524

Abstract

Aesthetic treatments related to the perfect smile are important in contemporary clinical dentistry. The disharmony of the smile, often called the gummy smile, can be caused by vertical maxillary growth, dentoalveolar extrusion, short upper lip, hyperactivity of the upper lip, altered passive eruption (APE), or a combination of such factors. Some studies mention the possibility of APE being a risk factor for periodontal disease. In some cases, excessive gingival can interfere with oral hygiene procedures and result in plaque accumulation and inflammation of the gingival margin. The management of APE is by periodontal crown lengthening surgery. The selection of the crown lengthening technique is adjusted to the classification, thickness, and number of keratinized gingiva, as well as the location of the cementoenamel junction (CEJ) or bone peak against the gingival margin.
Keterkaitan antara Halitosis dengan Bakteri Penyebab Periodontitis Luki Astuti; Olivia Nauli Komala
Jurnal Kedokteran Gigi Terpadu Vol. 5 No. 1 (2023): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v5i1.17174

Abstract

Halitosis, often known as foul breath, refers to an unpleasant odor that emanates from the mouth cavity when inhaling or talking. Halitosis can have a negative impact on a person's quality of life, including symptoms of depression or anxiety, low self-esteem, embarrassment, prejudice, and social isolation. Bad breath can be caused by both intra oral and extra oral factors. Bacterial growth in the oral cavity is excellent for the production of volatile sulfur compounds (VSC). The posterior dorsum of the tongue, gingival sulcus, periodontal pockets, and gingival embrasures are all places where bacterias can accumulate. Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, and Treponema dentiocola are known to actively produce VSC through interactions with specific substrates such as cysteine, methionine, tryptophan, arginine, and lysine to produce hydrogen sulfide (H2S) and methylmercaptan (CH3SCH3). However, the presence of VSC does not indicate the presence of periodontitis, as any space in the oral cavity that can cause the retention of bacteria/biofilms can become a site for bacterial decomposition, thereby facilitating the growth of VSC. Tongue cleansing is proven to help reduce the formation of VSC.
Pendidikan dan Pelatihan Komunitas WKRI Ranting Birgitta untuk Peningkatan Kesehatan Rongga Mulut Firstine Kelsi Hartanto; Rahmi Amtha; Trijani Suwandi; Komariah; Luki Astuti; Andri Sutanto; Caroline Septiani; Jacinda Jesslyn; Nikita Theodorus; Elisabeth Sudarmi; Indrayadi Gunardi
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 2 No. 1 (2023): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v2i1.17260

Abstract

The oral cavity is a mirror of a person's health that can show local and systemic health problems. Dental and oral health problems, especially caries, periodontal disease, and cancer are reported as diseases and disorders in dental and oral health that are still experienced by the population in the world and Indonesia. It is known from a preliminary survey, that family members of WKRI community partner Ranting Birgitta have quite a lot of oral health problems but cannot freely come to the dentist for treatment during the pandemic. The aim of this community service was (1) to increase the knowledge about the problem of caries, bleeding gums, bad breath and the prevention (2) to increase the knowledge of causes and manifestations of various disorders that arise due to systemic medications and how to overcome them. The activity was carried out offline through the educational materials in the form of presentations, demonstrations, and posters to members of the WKRI community of Birgitta branches and guests, on February 5, 2023, at Grha St. Mikael, Kranji, Bekasi. A total of 43 people (32 participants and 11 PkM teams) participated in this activity. The pre-test and post-test results attended by 29 participants showed a significant increase in knowledge by 1.2 times (p<0.001) of the material presented, with an average pre-test score of 54.66 and 78.10 for the average post-test score. Overall, the activity went smoothly. It is highly suggested that similar activities can be held again with a wider scope of participants.
MANIFESTASI LESI YANG SANGAT TERKAIT DENGAN HIV/AIDS PADA JARINGAN PERIODONTAL Luki Astuti; Olivia Nauli Komala
JURNAL PENELITIAN DAN KARYA ILMIAH LEMBAGA PENELITIAN UNIVERSITAS TRISAKTI Vol. 8 No. 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pdk.v8i2.15895

Abstract

Infeksi Human Immunodeficiency Virus (HIV) merupakan infeksi kronis yang ditandai dengan fase asimtomatik selama bertahun-tahun. HIV mengakibatkan penderita kehilangan daya tahan tubuh sehingga mudah terinfeksi. Apapila penderita tidak mendapatkan perawatan, HIV dapat berlanjut menjadi Acquired Immuno Deficiency Syndrome (AIDS) yang ditandai dengan berkembangnya berbagai infeksi, kanker, atau manifestasi klinis jangka panjang. Selain penyakit sistemik yang timbul pada pasien HIV/AIDS, lesi oral juga sering ditemukan akibat perkembangan dari infeksi oportunistik. Menurut klasifikasi EC Clearinghouse, lesi pada jaringan periodontal termasuk kedalam kelompok 1 yaitu lesi yang sangat terkait dengan HIV/AIDS. Lesi periodontal tersebut antara lain linear gingival erythema (LGE), necrotizing periodontal disease yang terdiri dari necrotizing ulcerative gingivitis dan necrotizing ulcerative periodontitis (NUG, NUP). Dokter gigi sebagai pemberi pelayanan kesehatan gigi layaknya memiliki pengetahuan yang baik mengenai manifestasi HIV/AIDS pada jaringan periodontal. Manifestasi oral seringkali merupakan indikator paling awal dari infeksi HIV dan dapat meramalkan perkembangan HIV/AIDS.