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Utilization Pemanfaatan Ekstrak Kulit Ubi Jalar Ungu Sebagai Pengganti Crystal Violet pada Pewarnaan Gram Andi Sri Nurul Hidayanti; Sulfiani Sulfiani; Nuramaniyah Taufiq
Jurnal Sehat Mandiri Vol 16 No 2 (2021): Jurnal Sehat Mandiri, Volume 16, No.2 Desember 2021
Publisher : Poltekkes Kemenkes Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.063 KB) | DOI: 10.33761/jsm.v16i2.364

Abstract

Purple sweet potato has the high anthocyanin content. The anthocyanin content in purple sweet potato skin is higher than the anthocyanin in the tubers, namely 52.84-729.74 mg/100g while the tubers are 110.51 mg/100g. Natural dyes that have the potential to be extracted include anthocyanins. This research aimed to test anthocyanin dyes in purple sweet potato peel extract as the substitute for crystal violet in gram staining. The type of research used Quasi Experiments. This research was conducted in the Microbiology Laboratory of the Hasanuddin University Medical Faculty. In this research, extraction was carried out by using the solvent of Ethanol : Acetic Acid : Water (25: 1: 5). Furthermore, gram staining was carried out by using purple sweet potato peel extract using a concentration of 60%, 70%, 80%, 90%, 100% and crystal violet as positive control. The results of this research indicate that the staining used purple sweet potato peel extract concentrations of 60%, 70%, 80%, 90% in gram-positive Staphylococcus bacteria isolates showed poor bacterial staining results because the bacteria were not purple when compared to crystal violet dye. Where as at the concentration of 100% it showed good bacterial staining results because the bacteria was purple. Meanwhile, gram-negative e.coli bacteria isolates at the concentration of 60%, 70%, 80%, 90%, 100% showed good bacterial staining results because the bacteria were red. Suggestions for further research to do maceration longer.
Pengaruh Waktu Perendaman Perasan Jeruk Nipis (Citrus aurantium) Terhadap Penurunan Kadar Formalin pada Udang Rebon Rosdiana Mus; Sulfiani Sulfiani; Nisfa Musdalifah
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 8, No 2 (2021): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v8i2.14073

Abstract

Formalin sebagai antibacterial agent pada makanan yang mengandung banyak protein. Salah satu makanan yang dicurigai mengandung formalin yaitu udang rebon karena memiliki kandungan protein yang tinggi. Senyawa alami yang dapat menurunkan kadar formalin pada udang seperti senyawa saponin dapat ditemukan dalam jeruk nipis (Citrus aurantium). Tujuan penelitian yaitu menentukan kadar formalin pada udang rebon sebelum dan setelah perendaman dengan perasan jeruk nipis berdasarkan lama perendaman 80, 100, dan 120 menit dan mengetahui pengaruh waktu perendaman untuk menurunkan kadar formalin pada udang rebon. Metode penelitian yaitu metode semi kuantitatif (Strip Formaldehid) dan metode kuantitatif (Spektrofotometri UV-Vis). Hasil dari penelitian ini yaitu pada sampel A1 (80 menit) = 0,0321% b/v, sampel A2 (100 menit) = 0,0308% b/v dan sampel A3 (120 menit) = 0,0298% b/v. Penurunan kadar formalin paling tinggi yaitu pada perendaman selama 120 menit dengan konsentrasi awal yaitu 0,0826% b/v turun menjadi 0,0298% b/v setelah perendaman dengan perasan jeruk nipis 6% dengan penurunan kadar formalin sebanyak 0,0528% b/v. Semakin lama waktu perendaman maka semakin menurun kadar formalin pada udang rebon karena senyawa saponin yang terkandung di dalam jeruk nipis mampu berikatan dengan air dan formalin.
PEDULI COVID-19 BAGI ANAK-ANAK OTG (ORANG TANPA GEJALA) DI TEMPAT KARANTINA DUTA COVID-19 ALMADERA HOTEL Andi Meinar Dwi Rantisari; Sulfiani Sulfiani
Batara Wisnu : Indonesian Journal of Community Services Vol. 1 No. 1 (2021): Batara Wisnu | Januari - April 2021
Publisher : Gapenas Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.362 KB) | DOI: 10.53363/bw.v1i1.39

Abstract

During the COVID-19 outbreak, a social phenomenon emerged that could potentially exacerbate the situation: the emergence of social stigma and discrimination against COVID-19 patients, not to mention children with OTG status in quarantine. This situation can trigger anxiety and stress, which can have an impact on their immunity. In addition, children with OTG status in the quarantine may be vulnerable to stress because environmental changes and activity restrictions can make children easily psychologically depressed. The purpose of this Activity is to provide donations to OTG children at the Covid 19 Ambassador Quarantine at the Almadera Makassar Hotel and as a form of support for the government in tackling the COVID 19 pandemic in Indonesia, especially in the city of Makassar, South Sulawesi. The steps taken in this Activity began with Opening, Providing donations in storybooks, toys, and milk, and Distract Activity which involved the COVID 19 Ambassador Task Force Team of the South Sulawesi Provincial Health Office and lecturers at Megarezky University. Based on the results of the implementation of the community service program that this Activity went well and smoothly. This is known from the level of attendance and Activity of the participants. The total participants were 19 people, OTG children, and volunteers from the COVID 19 Task Force who served at the Almadera Hotel. The participants who attended were very enthusiastic. The result of this Activity is that it is hoped that it can increase awareness of the survivors of COVID 19 so that they can increase their enthusiasm and immunity so that they recover quickly and return home
PEMERIKSAAN GOLONGAN DARAH PELAJAR TAMAN KANAK-KANAK ISLAM TERPADU (TKIT) AL QALAM MAKASSAR Sulfiani Sulfiani; Basmiati Basmiati; Nuralam Sobak; Riski Handayani
Batara Wisnu : Indonesian Journal of Community Services Vol. 1 No. 1 (2021): Batara Wisnu | Januari - April 2021
Publisher : Gapenas Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.586 KB) | DOI: 10.53363/bw.v1i1.73

Abstract

Knowing blood type is an important part for every human being. Many of us do not know what type of blood type we have. This case becomes very crucial when the individual experiences a disaster. The action of handling the patient becomes late, because this individual does not yet know the type of blood group he has. Knowing your blood type helps the body stay healthy, plan for the future and prevent complications. The implementation method in this community service activity begins with the planning stage by conducting site surveys and interviews with the school, the preparation stage by preparing equipment in the form of tools, materials and other equipment and the implementation stage by opening and checking blood groups and distributing blood group identity cards. This activity was well received by the school and students. The number of students who carried out the examination was 26 people with a percentage of 42.31% male students and 57.69% female students. The results of the blood group examination obtained by the students of TKIT Al-Qalam Makassar are having blood group A (42.31%), blood type B (26.92%), blood type AB (7.69%) and blood type O (42, 31%).
Pemanfaatan Ekstrak Bunga Mawar Merah (Rosa hybrida) Asal Desa Bonto Majannang Kabupaten Bantaeng sebagai Indikator Formalin pada Ikan Asin Sulfiani Sulfiani; Sukmawati Sukmawati
Jurnal Abdidas Vol. 1 No. 5 (2020): Vol 1 No 5 October Pages 306-486
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdidas.v1i5.99

Abstract

Banyaknya penggunaan pengawet bahan makanan yang seharusnya tidak diperuntukkan untuk bahan pangan seperti formalin menjadi keresahan bagi masyarakat. Penggunaan pengawet formalin pada umumnya digunakan pada bahan makanan sumber protein seperti pada ikan asin. Selama ini indikator yang digunakan untuk deteksi formalin yaitu menggunakan strip formaldehid dan larutan kalium permanganat yang dapat diperoleh di toko bahan kimia yang biaya cukup mahal. Salah satu alternatif lain untuk deteksi adanya formalin dapat menggunakan zat pewarna alami dari senyawa antosianin. Senyawa antosianin dapat diperoleh pada ekstrak bunga mawar merah. Di Desa Majannang Kabupaten Bantaeng hampir semua warga memiliki tanaman bunga mawar merah yang hanya dijadikan sebagai penghias taman. Untuk itu dengan kandungan yang terdapat pada bunga mawar merah dapat berfungsi sebagai zat pewarna alami antosianin yang dapat digunakan sebagai indikator deteksi formalin. Metode yang digunakan untuk pembuatan indikator formalin yaitu dengan cara ekstraksi dingin (maserasi) menggunakan larutan etanol 96% selama 3 hari untuk memperoleh ekstrak kental antosianin kemudian dibuat menjadi strip formalin menggunakan kertas saring whatman. Hasil yang diperoleh menggunakan indikator kertas formalin ekstrak bunga mawar merah yang diujikan ke sampel ikan asin yaitu indikator kertas yang telah dibuat berwarna merah muda dan jika di celupkan ke dalam sampel postif, indikator kertas tersebut warnaya tetap merah dan jika sampel negatif mengandung formalin maka indikator berubah warna ungu. Sehingga dapat disimpulkan bahwa indikator kertas ekstrak bunga mawar merah dapat dijadikan sebagai indikator formalin.
THE ANALYSIS OF LEAD CONTENT (Pb) IN CRABS (portunus pelagicus) IN THE COASTAL WATERS OF MA’RANG SUB-DISTRICT OF PANGKEP DISTRICT sulfiani ani Hasan
Jurnal Inovasi Penelitian Vol 1 No 6: Nopember 2020
Publisher : Sekolah Tinggi Pariwisata Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47492/jip.v1i6.188

Abstract

The waters of Ma'rang Subdistrict, Pangkep Regency, have the potential to be contaminated with heavy metal lead (Pb), which is used as a place for fishing and marine products. The use of gasoline by boats as a means of transportation for looking for small crabs (Portunus Pelagicus), farming seaweed, or other marine products are thought to cause heavy metal lead (Pb) in these waters. The purpose of this study was to determine the contamination of the heavy metal lead (Pb) in the crab (Portunus Pelagicus) and to determine the content of the heavy metal lead (Pb) in the crab (Portunus Pelagicus). The method of this research was the Atomic Absorption Spectrophotometer (AAS) by analyzing the lead (Pb) content of the crab (Portunus Pelagicus) obtained in the waters around the jetty in the coastal area of ​​Ma'rang District, Pangkep Regency. The results showed that the lead content (Pb) in the crab (Portunus Pelagicus) in the coastal waters of Ma'rang District, Pangkep Regency, for samples from Bawa Salo Pier, the lead content was 0.0059 μg/g, the sample from Pungkalawaki Pier obtained a lead range of 0.0796 μg/g, samples from Dusunge Wharf obtained lead levels of 0.0236 μg / g, samples from Jennae Wharf obtained lead levels of 0.0064 μg/g, and samples from Kessi Wharf obtained lead levels reaching 0.0944 μg/g. Based on BPOM Regulation Number 5 of 2018, the lead content obtained from crabs (Portunus Pelagicus) is still below the threshold of 0.20 μg/g
Analysis Of Lead Levels (Pb) In Public Transport Drivers’hairbyusing Automic Absorption Spectrophotometer (AAS) Method Wini Alimudin; Sulfiani Sulfiani; Julia F. N
Jurnal Media Analis Kesehatan Vol 12, No 2 (2021): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mak.v12i2.2336

Abstract

Lead levels (Pb) is a heavy metal that is toxic to humans. There are many people do their activites on the highway, one of them is a public transport driver. Public transport driversare works who have a considerable by using an Atomic Absorption Spectrophotometer (AAS). The aim of research was todetermine the levels of lead (Pb) in the hair of public transport  driver. The number of samples was 11 respondents of public transport drivers by using accidental sampling technique. Based on the research results found that the concentration of heavy metal lead (Pb) in hair of public transport drivers. The results shows that sample code (A) with thelength of working was 20 years, the Pb level : 65.10 µg/g; sample (B) with thelength of working was 30 years, Pb level : 91.44 µg/g; sample (C) with thelength of working was 15 years, Pb level : 39.11 µg/g; sample (D) with thelength of working was 14 years, Pb level : 39.37 µg/g; sample (E) with thelength of working was 3 years, Pb level : 12.20 µg/g; sample (F)with thelength of working was 7 years Pb level : 30.26 µg/g; sample (G) with thelength of working was 9 years Pb level : 15.90 µg/g; sample (H) with thelenght working was 15 years Pb level : 40.98 µg/g; sample (I) with thelenght working was 10 years Pb level : 74.55 µg/g; sample (J) with thelenght working was 10 years, Pb level : 36.50 µg/g; sample (K) with thelenght working was 6 years, Pb level : 18.60 µg/g. It can be concluded that the lead levels (Pb) in all research samples have Pb levels exceeding the threshold value, where the maximum tolerance limit for lead levels in hair based on WHO is ≤ 12 µg/g.
Identifikasi Jamur Dermatofita Penyebab Tinea unguium Pada Kuku kaki Petani di Dusun Ballakale Desa Aska Kecamatan Sinjai selatan Kabupaten Sinjai: Identifikasi Jamur Dermatofita Penyebab Tinea unguium Pada Kuku kaki Petani di Dusun Ballakale Desa Aska Kecamatan Sinjai selatan Kabupaten Sinjai Nurfadillah, Hartati, Sulfiani
Kampurui Jurnal Kesehatan Masyarakat Vol. 3 No. 2 (2021): Kampurui Jurnal Kesehatan Masyarakat
Publisher : Fakultas Kesehatan Masyarakat Universitas Dayanu Ikhsanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55340/kjkm.v3i2.498

Abstract

Tinea unguium is an infection of the nail plate caused by the fungus Dermatophyta. Themajority of the livelihood in Dusun Ballakale Desa Aska Kecamatan Sinjai selatan Kabupaten Sinjai is farming where the habit of farmers in this area is to work on rice fields that are in direct contact with soil, water and mud for a long time without wearing protective equipment, one of which is boots to protect the feet. Nails that are in direct contact with soil or mud for a long time and are rarely cleaned can trigger fungal growth. The purpose of this study was to determine the presence or absence of a dermatophyte fungus that causes tinea unguium on farmers' toenails and to determine the type of dermatophyte fungus that causes tinea unguium on farmers' toenails. The research method used is categorical descriptive with 10 samples examined. Meanwhile, the examination method in this research is using direct microscopic method with KOH 20 % and culture using Sabouraud Dextrose Agar (SDA) media. The results of the direct examination with KOH 20 % obtained 2 positive samples with the discovery of hyphae. And the results of the culture examination showed dermatophyte fungi in 5 samples of the Trichophyton rubrum type melanoid, Microsporum audouinii, and Epidermophyton floccosum, 3 samples of the non-dermatophyte fungus Aspergillus flavus, and 2 samples were not identified.
Pengaruh Penambahan Zat Kapur dan Lama Perendaman Terhadap Kadar Sianida pada Singkong (Manihot Esculanta Crantz) Sulfiani Ani Hasan; Nuramaniyah Taufiq
Jurnal Sehat Mandiri Vol 17 No 2 (2022): Jurnal Sehat Mandiri, Volume 17, No.2 Desember 2022
Publisher : Poltekkes Kemenkes Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33761/jsm.v17i2.815

Abstract

Cassava (Manihot esculenta Crantz) or also known as cassava has a high carbohydrate content and has become the third staple food source after maize and rice. Cassava also contains linamarin and lotaustralin poisons which belong to the cyanogenic glycoside group. Cyanogenetic glycosides are compounds found in plant foodstuffs and are potentially very toxic because they decompose and release hydrogen cyanide. This study aims to determine the cyanide acid content in cassava before being soaked in lime solution and to determine the cyanide acid content in cassava after soaking in lime solution with concentrations of 0.25%, 0.50%, 0.75%, 1% and 2% for 2 hours, 4 hours, and 6 hours. Using argentometry method. Soaking with lime concentrations of 0.25%, 0.50%, 0.75%, 1% and 2% does not affect the cyanide acid levels, which affects the cyanide levels, namely the long soaking time which is 2 hours can reduce the cyanide acid levels. up to 50%, soaking time of 4 hours reduced 62.5% and soaking time of 6 hours reduced up to 75% the level of cyanide acid contained in cassava. Based on the research, it can be concluded that the soaking time greatly affects the decrease in cyanide levels found in cassava. Suggestions should try measurements using instrumentation methods.
Formulation and Test of Antibacterial Activity Gel Mask Peel Off Stem and Suruhan Leaves (Peperomia Pellucida L.) Extract Against Propionibacterium Acne Nurfiddin Farid; Andi Meinar Dwi Rantisari Thayeb; Sulfiani Sulfiani; Adriandy Saleh
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.849 KB)

Abstract

Stems and leaves of suruhan (Peperomia Pellucida L.) contain compounds belonging to the class of flavonoids, alkaloids, tannins, phenols, terpenes and essential oils which have potential as antibacterials. This study aimed to find out whether the stem and leaf extracts of suruhan (Peperomia pellucida L.) can be formulated into a gel peel-off mask preparation that is physically and chemically stable, and can inhibit the acne-causing bacteria Propionibacterium acne. This study used a laboratory experimental method, namely the formulation of Peel off gel mask preparations of stem and leaf extracts with different base concentration variations. Testing the characteristics of the preparation which includes organoleptic test, homogeneity, spreadability test, pH test, drying time test, viscosity test using the cycling test method and for antibacterial activity test using the well diffusion method. The results showed that the gel peel-off mask preparation of suruhan stem and leaf extract (Peperomia pellucida L.) fulfilled the requirements for the characteristics of the preparation both physically and chemically, and could inhibit the growth of acne-causing bacteria (Propionibacterium acne) at a concentration of 2% (15.1 mm), 3%, (17.8 mm) and 4% (19.4 mm) in the zone of strong inhibition. It can be concluded that formulation gel mask preparations Peel off stems and leaves of suruhan (Peperomia pellucida L.) can provide antibacterial activity against Propionibacterium acne with respective inhibition zones of 15.1 mm, 17.8 mm and 19.4 mm which are equally strong in inhibiting Propionibacterium acne bacteria