Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Ence Darmo Jaya Supena
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Bogor Agricultural University, Bogor 16680, West Java Research Center For Bioresources And Biotechnology, Bogor Agricultural University, Bogor 16680, West Java
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Induction of Somatic Embryogenesis in Sengon (Falcataria moluccana) With Thidiazuron and Light Treatments Ari Sunandar; . Dorly; Ence Darmo Jaya Supena
HAYATI Journal of Biosciences Vol. 24 No. 2 (2017): April 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (992.467 KB) | DOI: 10.4308/hjb.24.2.105

Abstract

Falcataria moluccana is important for reforestation and afforestation in Indonesia. However, epidemic of gall rust disease in F. moluccana plantations decreases its productivity. Genetic engineering is an alternative solution to against gall rust disease. Somatic embryogenesis is an efficient in vitro plant regeneration for successful plant improvement through genetic engineering. The objective of this study was to investigate the effect of thidiazuron and light treatments on the induction of somatic embryogenesis of F. moluccana. The effects of thidiazuron concentration (5, 10 or 15 μM) and light (continuous light, 7 days of dark followed by light, or continuous dark) on the induction of somatic embryogenesis in leaf explants were assessed. The highest production of somatic embryos was obtained in 5 μM thidiazuron and dark treatments for 7 days followed by light in Murashige and Skoog medium supplemented with 1.2 g/L proline. Histological analysis in globular and cotyledon stages confirmed that cells had progressed to secondary somatic embryogenesis. This research needs more improvements to become a successful and efficient somatic embryogenesis method and as a potential method for successful plant improvement through genetic engineering in F. moluccana.
Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF SEAWEED Kappaphycus alvarezii USING Gα GENE AND CALLUS CULTURES Erina Sulistiani; Suharsono Suharsono; Ence Darmo Jaya Supena; Miftahudin Miftahudin
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 1 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.176 KB) | DOI: 10.11598/btb.2019.26.1.989

Abstract

Cottonii seaweed (Kappaphycus alvarezii Doty) is one of the most important commercial sources of carrageenans which are widely used in the pharmaceuticals and food industries. A problem in the cultivation of this seaweed is the ice-ice disease, which is caused by extreme changes in environmental conditions such as temperature and seawater salinity. Gene transformation to produce Cottonii seaweed transgenics that are tolerant to environmental stress is a potential solution to this problem. Gα gene encodes for the heterotrimeric G protein α subunit is a gene that plays a role in tolerance to biotic and abiotic environmental stresses. This study aimed to: (a) introduce the Gα gene into the callus cells of K. alvarezii and regenerate transformed callus cells to transgenic plantlets; (b) determine the appropriate concentration of acetosyringone and Agrobacterium tumefaciens strain for gene transfer into the callus of K. alvarezii. The callus cells of K. alvarezii were transformed using Agrobacterium tumefaciens strains LBA4404 and EHA105 carrying the expression vector pGWB502-Gα with a CaMV-35S promoter. The calli and A. tumefaciens were co-cultivated in several concentrations of acetosyringone (20, 40, 60 mg/L). The regeneration of transformed callus cells into transgenic plantlets was successfully performed using the somatic embryogenesis technique. The results showed that the highest percentage of putative transgenic micropropagule formation occurred at the 20-40 mg/L concentration of acetosyringone. Polymerase chain reaction (PCR) analysis on the twenty transgenic plantlets indicated that the Gα gene was successfully introduced into the genomic DNA of all of them. The highest transformation efficiency was in the co-cultivation treatment of 20-40 mg/L acetosyringone (22-28%). The transformation efficiency produced by Agrobacterium tumefaciens EHA105 (23%) was not significantly different from that produced by the LBA4404 (15%).
Co-Authors Dorly Dorly Erina Sulistiani Miftahudin . Suharsono Suharsono Sunandar, Ari