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All Journal HAYATI Journal of Biosciences Indonesian Journal of Cancer Chemoprevention Universa Medicina Molecular and Cellular Biomedical Sciences (MCBS)
Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay Wahyu Widowati; Diana Krisanti Jasaputra; Teresa Liliana Wargasetia; The Fransiska Eltania; Alya Mardhotillah Azizah; Mawar Subangkit; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Ermi Girsang; Ahmad Faried
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.186

Abstract

Breast cancer (BC) is the number one cause of deaths from cancer in women. Metastasis in BC is caused by immunosurveillance deficiency, including impairment of Natural Killer (NK) cell maturation, low NK activity, and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukin 15 (IL15) against BC cells. Human recombinant IL15 was used to induce NK cells. To evaluate the potential of IL15 in inducing NK cells, we measured the activating and inhibiting receptors (NKG2D, NKG2A), apoptotic potency of NK cells on BC cells (MCF7) using transwell assay. The IL15 inducer on the NK cell were measured NKG2D, NKG2A gene expression with quantitative polymerase chain reaction (qPCR), (GzmB) secretion using ELISA, apoptotic gene expression of MCF7 using qPCR. IL15 increased NKG2D expression 4.01-9.13%, but IL15 could not affect toward NKG2A expression on NK cells. IL15-activated NK cells, inhibited BC cells proliferation, induced apoptotic BC cells 25.89-32.19%, induced apoptotic genes of BC cells bax, p53. IL15 increase NK activating receptor (NKG2D), inhibit BC cells proliferation, induce apoptotic percentage and induce apoptotic gene expression.
Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro Chandra Risdian; Wahyu Widowati; Tjandrawati Mozef; Teresa Liliana Wargasetia; Khie Khiong
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss1pp141-145

Abstract

Various diseases including cancer and cardiovascular diseases are induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in the cells. Piper betle L., commonly called betel, belongs to the Piperaceae family. It originated from South and South East Asia, and has been used as a traditional medicine by people there due to its medicinal properties. In the present study, free radical scavenging activity of ethanolic leaves extract of Piper betle L. together with its different solvent fractions (hexane, ethyl acetate, butanol, and water) were evaluated by DPPH free radical scavenging assay. The ethanolic extract and the ethyl acetate fraction had shown very significant DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity compared to the other fractions. In DPPH free radical scavenging assay, the IC50 value of the ethanolic extract and the ethyl acetate fraction were found to be 17.43µg/mL and 11.53 µg/mL, respectively. The free radical scavenging activity was assumed due to the total phenolic content. The total phenolic content was found to be highest in the ethanol extract (277.68 μg EGCG/mg) and the ethyl acetate fraction (559.38μg EGCG/mg). The results concluded that the ethanolic leaves extract of Piper betle L. and its ethyl acetate fraction have a considerably free radical scavenging activity.Keywords: Piper betle L., Piperaceae, free radical scavenging, DPPH
Annona muricata leaves have strongest cytotoxic activity against breast cancer cells Susi Endrini; Suherman Suherman; Wahyu Widowati
Universa Medicina Vol. 33 No. 3 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.179-184

Abstract

BACKGROUND Plant-derived herbal compounds have a long history of clinical use, better patient tolerance and acceptance. They are freely available natural compounds that can be safely used to prevent various ailments. Plants have been the basis of traditional medicine throughout the world for thousands of years and are providing mankind with new remedies. The objective of this study was to determine the cytotoxicity of soursop (Anona muricata Linn) leaves and pearl grass (Hedyotis corymbosa (L.) Lam.) on the hormone-dependent human breast carcinoma Michigan Cancer Foundation-7 (MCF-7) cell line. METHODS This study used two types of solvents (water and ethanol) in the extraction process and two incubation times (24 hours and 48 hours) in the MTT assays to analyze the cytotoxic effects of both plants. RESULTS Preliminary results showed that the ethanolic extract of soursop leaves (SE) displayed cytotoxic effects against MCF-7 on 24- and 48-hour incubation times with IC50 values of 88.788 μg/ml and 14.678 μg/ml, respectively. Ethanolic pearl grass extract (PE) showed similar results, with IC50 values of 65.011 μg/ ml on 24-hour incubation time and 52.329 μg/ml on 48-hour incubation time against MCF-7 cell line. However, the water extract of both plants displayed lower cytotoxic effect against MCF-7 cell line. CONCLUSION The ethanolic extract of both plants displayed cytotoxic effect against MCF-7. Soursop (Anona muricata Linn) leaves have the strongest cytotoxic activity against MCF-7 breast cancer cell line.
Comparison of Antiaging and Antioxidant Activities of Protocatechuic and Ferulic Acids Ermi Girsang; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Maulidwina Bethasari; Annisa Amalia; Wahyu Widowati
Molecular and Cellular Biomedical Sciences Vol 4, No 2 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1345.012 KB) | DOI: 10.21705/mcbs.v4i2.90

Abstract

Background: Skin-aging is a progressive changes in the skin combine with cumulative extrinsic factors which are mostly caused by free radicals caused by exposure to lots of free radicals molecules from pollutant, wrongly food intake, or too much sun bathing. These free radicals can be tackled by a treatment using antioxidants. Prevention of aging can be done by escalating antioxidant intake. Protocatechuic acid (PCA) and Ferulic acid (FA) have been known for their scavenging properties on free radicals and antiaging activity. Antioxidant and antiaging activity of both compounds have not been compared comprehensively before. Hence, current study was conducted to compare the potential of PCA and FA for their antioxidant and antiaging activities using various methods.Materials and Methods: Antioxidant analysis of PCA and FA was conducted using H2O2 scavenging assay, 2,2’-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazil (DPPH), and ferric reducing antioxidant power (FRAP). Meanwhile, antiaging activities of PCA and FA were examined using inhibitory activities of tyrosinase, collagenase, elastase, hyaluronidase and tyrosinase.Results: IC50 of scavenging activity of ABTS were 125.18 µg/mL (PCA) and 35.55 µg/mL (FA), inhibition activity of collagenase were 126.16 µg/mL (PCA) and 52.85 µg/mL (FA) and inhibition activity of tyrosinase were 246.42 µg/mL (PCA), 253.58 µg/mL (FA).Conclusion: In conclusion, FA has better ABTS scavenging and collagenase inhibition activities compared to PCA. Meanwhile, PCA has better activity of tyrosinase inhibition than FA.Keywords: antioxidant, antiaging, ferulic acid, protocatechuic acid
Allogeneic Mesenchymal Stem Cells and Its Conditioned Medium as a Potential Adjuvant Therapy for COVID-19 Wahyu Widowati; Ahmad Faried; Hanna Sari Widya Kusuma; Yulius Hermanto; Ali Budi Harsono; Tono Djuwantono
Molecular and Cellular Biomedical Sciences Vol 7, No 1 (2023)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v7i1.287

Abstract

Recent research has demonstrated that mesenchymal stem cells (MSCs) potentially benefit and enhance coronavirus disease (COVID-19) recovery. This benefit occurs via a mechanism that promotes viral clearance by phagocytes and macrophages. This action occurs through the innate (increase in IL-10 production and decrease in TNF-α and IL-12 production) and the adaptive immune system (decrease in IL-17 production, promote regulatory T cell proliferation and inhibit effectors T cell proliferation). MSCs are expected to act as an anti-inflammatory in the hyper-inflammatory state of COVID-19. MSCs enhance immune cell replacement that have been overwhelmed or have been lost due to cytokine storm. Although vaccines are the answer to this pandemic, MSCs can improve COVID-19 patients, especially in patients with chronic illnesses. The focus on keeping death-rates low is a great opportunity for MSCs-based therapy for severe or critically ill patients. MSCs and conditioned medium have the potential to serve as adjunctive therapy in preventing the body's overactive defense response or the so-called cytokine storm caused by COVID-19.Keywords: adjuvant therapy, COVID-19, mesenchymal stem cells, secretome
Co-Authors Ahmad Faried Ahmad Faried Ali Budi Harsono Alya Mardhotillah Azizah Annisa Amalia Chandra Risdian Chrismis Novalinda Ginting Diana Krisanti Jasaputra Ermi Girsang Hanna Sari Widya Kusuma I Nyoman Ehrich Lister Khie Khiong Maulidwina Bethasari Subangkit, Mawar Suherman Suherman Susi Endrini Teresa L. Wargasetia The Fransiska Eltania Tjandrawati Mozef Tono Djuwantono Yulius Hermanto