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All Journal HAYATI Journal of Biosciences Majalah Kedokteran Bandung Global Medical and Health Communication Molecular and Cellular Biomedical Sciences (MCBS)
I Nyoman Ehrich Lister
Faculty of Medicine, Universitas Prima Indonesia, Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay Wahyu Widowati; Diana Krisanti Jasaputra; Teresa Liliana Wargasetia; The Fransiska Eltania; Alya Mardhotillah Azizah; Mawar Subangkit; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Ermi Girsang; Ahmad Faried
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.186

Abstract

Breast cancer (BC) is the number one cause of deaths from cancer in women. Metastasis in BC is caused by immunosurveillance deficiency, including impairment of Natural Killer (NK) cell maturation, low NK activity, and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukin 15 (IL15) against BC cells. Human recombinant IL15 was used to induce NK cells. To evaluate the potential of IL15 in inducing NK cells, we measured the activating and inhibiting receptors (NKG2D, NKG2A), apoptotic potency of NK cells on BC cells (MCF7) using transwell assay. The IL15 inducer on the NK cell were measured NKG2D, NKG2A gene expression with quantitative polymerase chain reaction (qPCR), (GzmB) secretion using ELISA, apoptotic gene expression of MCF7 using qPCR. IL15 increased NKG2D expression 4.01-9.13%, but IL15 could not affect toward NKG2A expression on NK cells. IL15-activated NK cells, inhibited BC cells proliferation, induced apoptotic BC cells 25.89-32.19%, induced apoptotic genes of BC cells bax, p53. IL15 increase NK activating receptor (NKG2D), inhibit BC cells proliferation, induce apoptotic percentage and induce apoptotic gene expression.
Anti-Aging Activities of Asparagus Gel Ethanol Extract in Cosmetic Gel Agent for Facial Skin Henny Safrita Ginting; Edy Fachrial; I Nyoman Ehrich Lister; Adek Amansyah
Majalah Kedokteran Bandung Vol 53, No 3 (2021)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v53n3.2314

Abstract

Asparagus is a vegetable that contains phenolic compounds with antioxidant properties that scavenges aging-triggering free radicals. This study aimed to investigate the components and anti-aging potentials of Ethanol Extract form Asparagus (EEA). The study was performed in February 2020 at the Pharmacy Laboratory, University of North Sumatera. The EEA was obtained through maceration using 96% ethanol. An antioxidant assay was performed and the total phenol and flavodoid content were determined using the spectroscopic method. Three gel formulas with different concentrations of EEA was prepared (F1: 1.5%, F2: 2.5%, and F3: 3.5%), and F0 was used as control. The parameters evaluated were moisture, oil content, texture, collagen, wrinkle, pigment, sensitivity, and pore. The result showed that asparagus had a moderate antioxidant activity (IC50: 118,992) with the total phenol and flavonoid contents of 15,9407 mg GAE/g and 3,2286 mg QE/g extract, respectively. The highest aging activities was seen in F3 (3.5%), followed by F2 (2.5%) and F1 (1.5%). The percentage of moisture, oil, texture, collagen, wrinkle, spot, sensitivity, and pore recovery were found to be 40.15%, 49.73%, 71.76%, 17.70%, 70.93%, 49.34%, 42.56% and 25.31%, respectively. Hence, it can be concluded that the EEA Gel at the highest concentration (3.5%) has a high content of phenol and flavonoid which can improve the skin moisture, oil content, texture, collagen, wrinkles, spots, sensitivity, and pores, which promotes anti-aging activities.
Antioxidant and Anti-tyrosinase Activities of Aloe vera Rind and Gel Extracts Sonya Bonifacia Mahadi; Rr. Anisa Siwianti Handayani; Wahyu Widowati; Wilsen Wilsen; Yunita Dewani; Edy Fachrial; I Nyoman Ehrich Lister
Global Medical & Health Communication (GMHC) Vol 7, No 3 (2019)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.101 KB) | DOI: 10.29313/gmhc.v7i3.4453

Abstract

Aging is a natural process in human that can be characterized by the appearance of black spot on the skin due to hyperpigmentation. Aging may occur due to an excessive amount of free radicals in the body. Antioxidants possess ability to capture free radicals and inhibit tyrosinase which induces skin aging. Aloe vera has been used in traditional medicine because it contains several bioactive compounds that act as antioxidant and prevent aging process. This study aims to determine phytochemical content, antioxidant activity and tyrosinase inhibition activity of Aloe vera rind (AVRE) and gel (AVGE) extract. This research was carried out at the laboratorium of Aretha Medika Utama-Biomolecular and Biomedical Research Center in Bandung city in September–November 2018. Phytochemical assay was determined using modified Farnsworth method. Antioxidant assay was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and antiaging assay was obtained using tyrosinase inhibition assay. AVRE contains flavonoid, phenol, steroid, and alkaloid. Meanwhile, AVGE contains steroid and alkaloid. IC50 DPPH scavenging activity of AVRE was 113.18 µg/mL followed by AVGE was 291.96 µg/mL. IC50 tyrosinase inhibition activity of AVRE was 65.04 µg/mL followed by AVGE was 111.89 µg/mL. AVRE had more active DPPH scavenging activity and tyrosinase inhibition activity than AVGE. AKTIVITAS ANTIOKSIDAN DAN ANTITIROSINASE KULIT DAN DAGING LIDAH BUAYA (ALOE VERA)Penuaan merupakan proses alamiah pada manusia. Penuaan dapat terjadi akibat kadar radikal bebas yang tinggi di dalam tubuh. Antioksidan memiliki kemampuan memerangkap radikal bebas dan menghambat kerja enzim yang berperan dalam proses penuaan. Lidah buaya (Aloe vera) telah digunakan dalam pengobatan tradisional karena diketahui mengandung senyawa bioaktif yang bermanfaat dalam menangkal radikal bebas dan menghambat penuaan. Penelitian ini bertujuan mengetahui kandungan fitokimia dalam ekstrak etanol kulit lidah buaya (EKLB) dan ekstrak etanol daging lidah buaya (EDLB), mengetahui aktivitas antioksidan, pemerangkapan 2,2-difenil-1-pikrilhidrazil (DPPH), serta aktivitas antitirosinase EKLB dan EDLB. Penelitian ini dilakukan pada September–November 2018 di laboratorium Aretha Medika Utama Biomolecular and Biomedical Research Center (BBRC). Analisis fitokimia dilakukan menggunakan metode Farnsworth yang dimodifikasi. Aktivitas antioksidan dianalisis menggunakan metode pemerangkapan DPPH, sementara uji antipenuaan dilakukan menggunakan metode uji aktivitas antitirosinase. Hasil uji fitokimia menunjukkan EKLB mengandung flavonoid, fenol, steroid, dan alkaloid; sedangkan EDLB mengandung senyawa steroid dan alkaloid. EKLB dan EDLB memiliki aktivitas pemerangkapan DPPH dengan nilai IC50 secara berurutan 113,18 µg/mL dan 291,96 µg/mL. Penghambatan tirosinase EKLB dan EDLB dengan nilai IC50 secara berurutan 65,04 µg/mL dan 111,89 µg/mL. EKLB memiliki aktivitas antioksidan dan penghambatan tirosinase yang lebih baik dibanding dengan EDLB.
Antioxidant Activities of Ficus elastica Leaves Ethanol Extract and Its Compounds Chrismis Novalinda Ginting; I Nyoman Ehrich Lister; Ermi Girsang; Dewi Riastawati; Hanna Sari Widya Kusuma; Wahyu Widowati
Molecular and Cellular Biomedical Sciences Vol 4, No 1 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i1.86

Abstract

Background: The excessive free radicals condition called oxidative stress can harmful for the body. To prevent and cure it, the antioxidant agents are required. Nowadays, the natural product extracted from plants have been widely used in folk medicine as antioxidant for the treatment of many diseases. Ficus elastica (rubber tree) has some compounds that has several biological activities, i.e., quercitrin, myricitrin, morin, and eleutheroside B. The F. elastica works against the free radicals and can be potential as antioxidant agent. The purpose of this study was to evaluate antioxidant properties of F. elastica ethanolic extract (FEE), quercitrin, myricitrin, morin, and eleutheroside B.Materials and Methods: The antioxidant activities of FEE and standard compounds were evaluated by free radical-scavenging activity of 2,2-diphenyl-1-picrylhydrazil (DPPH), hydrogen peroxide (H2O2), 2,2’-azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) activities using spectrophotometry method.Results: FEE has the lowest of DPPH scavenging activity (IC50=13.82 µg/mL) than other compounds. In ABTS scavenging activity, FEE has moderate activity with IC50 value 23.29 µg/mL. In FRAP activity, FEE has moderate activity with value 241.58 µM Fe(II)/µg, while in H2O2 scavenging activity, FEE also show moderate activity with IC50=83.97 µg/mL compared to other compounds.Conclusion: In summary, FEE and the pure compounds (quercitrin, myricitrin, morin, and eleutheroside B) have potential as antioxidant agent.Keywords: free radical, morin, myricitrin, quercitrin, rubber tree, scavenging activities
Comparison of Antiaging and Antioxidant Activities of Protocatechuic and Ferulic Acids Ermi Girsang; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Maulidwina Bethasari; Annisa Amalia; Wahyu Widowati
Molecular and Cellular Biomedical Sciences Vol 4, No 2 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1345.012 KB) | DOI: 10.21705/mcbs.v4i2.90

Abstract

Background: Skin-aging is a progressive changes in the skin combine with cumulative extrinsic factors which are mostly caused by free radicals caused by exposure to lots of free radicals molecules from pollutant, wrongly food intake, or too much sun bathing. These free radicals can be tackled by a treatment using antioxidants. Prevention of aging can be done by escalating antioxidant intake. Protocatechuic acid (PCA) and Ferulic acid (FA) have been known for their scavenging properties on free radicals and antiaging activity. Antioxidant and antiaging activity of both compounds have not been compared comprehensively before. Hence, current study was conducted to compare the potential of PCA and FA for their antioxidant and antiaging activities using various methods.Materials and Methods: Antioxidant analysis of PCA and FA was conducted using H2O2 scavenging assay, 2,2’-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazil (DPPH), and ferric reducing antioxidant power (FRAP). Meanwhile, antiaging activities of PCA and FA were examined using inhibitory activities of tyrosinase, collagenase, elastase, hyaluronidase and tyrosinase.Results: IC50 of scavenging activity of ABTS were 125.18 µg/mL (PCA) and 35.55 µg/mL (FA), inhibition activity of collagenase were 126.16 µg/mL (PCA) and 52.85 µg/mL (FA) and inhibition activity of tyrosinase were 246.42 µg/mL (PCA), 253.58 µg/mL (FA).Conclusion: In conclusion, FA has better ABTS scavenging and collagenase inhibition activities compared to PCA. Meanwhile, PCA has better activity of tyrosinase inhibition than FA.Keywords: antioxidant, antiaging, ferulic acid, protocatechuic acid
Chemical Constituents of Snake Fruit (Salacca zalacca (Gaert.) Voss) Peel and in silico Anti-aging Analysis Ermi Girsang; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Adrian Khu; Butter Samin; Wahyu Widowati; Satrio Wibowo; Rizal Rizal
Molecular and Cellular Biomedical Sciences Vol 3, No 2 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1926.128 KB) | DOI: 10.21705/mcbs.v3i2.80

Abstract

Background: Skin aging is a condition where skin is unable to retain both its physiological and structural integrity. Plants is the main source of phtytochemicals compound with wide range of biological activities. Through the efforts of ongoing scientific researches, an increasing number of plant extracts and phytochemicals have been showed promising result as anti-aging agent. Snake fruit (Salacca zalacca (Gaert.) Voss) is tropical plant belongs to the palm tree family (Arecaceae) that served as important crop in Indonesia. Despite its utilization, the phytochemical compound available in snake fruit, especially its peel have not been well documented. Present study aimed to elucidate the phytochemical constituent of snake fruit peel and its anti-aging potency.Materials and Methods: Snake fruit peel extract (SPE) was subjected to qualitative phytochemical assay, high performance liquid chromatography, and molecular docking towards protein related in skin aging.Results: The screening showed SPE contained phytochemical compound belong to flavonoid, tannin, phenol, triterpenoid, saponin and alkaloid. Thus, based on the analysis only chlorogenic acid was present in SPE whilst rutin and caffeic acid were not detected. The SPE was contained chlorogenic acid around 1.074 mg/g dry weight. Chlorogenic acid had the high binding affinity towards matrix metalloproteinase (MMP)-1 (-9.4 kcal/mol).Conclusion: Current findings may provide scientific evidence for possible usage of SPE and its compounds as antioxidant and anti-aging agent.Keywords: Salacca zalacca, phytochemical compound, high performance liquid chromatography, anti-aging
Co-Authors Adek Amansyah Adrian Adrian Ahmad Faried Alya Mardhotillah Azizah Annisa Amalia Butter Samin Chrismis Novalinda Ginting Dewi Riastawati Diana Krisanti Jasaputra Edy Fachrial Ermi Girsang Hanna Sari Widya Kusuma Henny Safrita Ginting Maulidwina Bethasari Rizal Rizal Rr. Anisa Siwianti Handayani Satrio Wibowo Sonya Bonifacia Mahadi Subangkit, Mawar Teresa Liliana Wargasetia The Fransiska Eltania Wahyu Widowati Wahyu Widowati Wahyu Widowati Wahyu Widowati Wilsen Wilsen Yunita Dewani