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Pengendalian Penyakit Hawar Daun Phytophthora pada Bibit Kakao dengan Trichoderma asperellum Asti Irawanti Azis; Ade Rosmana; Vien Sartika Dewi
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.793 KB) | DOI: 10.14692/jfi.9.1.15

Abstract

Phytophthora leaf blight caused by Phytophthora palmivora is one constraint in cacao seedling in Indonesia. Generally, synthetic fungicides were applied to control this disease but its negative impact is becoming obvious recently. Research was conducted to evaluate the effectiveness of two Trichoderma asperellum isolates, ARBT-1 and ART-4, to suppress disease development. The two isolates were applied through seed treatment with spore concentrations of 104 mL-1, 105 mL-1, and 106 mL-1. The results showed that disease incidence on seedling treated with different spore concentrations of ARBT-1 was 39.0%, 23.6%, and 21.8%, respectively and those with ART-4 was 30.6%, 25.7%, and 30.8%, respectively, whereas disease incidence reached 90.3% for control at 21 days after inoculation of the pathogen. Observations on cocoa leaf tissues showed that the two isolates were found in leaf tissue, indicating that T. asperellum is an endophyte.Key words: antagonistic fungi, seedling, seed treatment
Pengendalian Penyakit Busuk Buah Phytophthora pada Kakao dengan Cendawan Endofit Trichoderma asperellum Andi Akbar Hakkar; Ade Rosmana; Muhammad Danial Rahim
Jurnal Fitopatologi Indonesia Vol 10 No 5 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.181 KB) | DOI: 10.14692/jfi.10.5.139

Abstract

The Phytophthora pod rot (PPR) caused by Phytophthora palmivora is one of important diseases affecting the decrease of cacao productivity. The use of endophytic Trichoderma have a big potential for controlling this disease. The rate of PPR incidence in twelve weeks after three times application by spraying of ART-4/G.J.S 09-1559 isolate of Trichoderma asperellum with concentration of 1 g L-1, 2 g L-1, 4 g L-1 was 5.4%, 5.3%, and 3.7%, respectively per week; while the rate of PPR incidence on control was 8.4% per week. Apparently healthy pods pretreated with above concentrations of T. asperellum in field showed 30, 0, and 0% infested by PPR on its surface, respectively after one week incubation in laboratory, compared to a 90% of surface infestation by PPR on control. After 12 week of inoculation, T. asperellum was recovered from pod tissue. This data demonstrated the potential of T. asperellum as bio control agent of PPR disease on cacao.  
Trichoderma Asal Akar Kopi Dari Alor: Karakterisasi Morfologi dan Keefektifannya Menghambat Colletotrichum Penyebab Penyakit Antraknosa secara in Vitro Didiana Yanuarita Molebila; Ade Rosmana; Untung Surapaty Tresnaputra
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.61-68

Abstract

Trichoderma of Coffee Roots From Alor: Morphological characteristic and in vitro Efficacy to Inhibit Colletotrichum Causing Anthracnose Trichoderma is a fungus capable of intimate associations with plant root systems including on coffee plants. This aim of study is to determine the characteristics of Trichoderma morphospecies from coffee roots of Alor origin, East Nusa Tenggara (NTT) and its ability to inhibit the growth of Colletotrichum causing anthracnose disease in-vitro. Root samples of healthy coffee plants were taken from the location of coffee plantations in Alor District, NTT. Isolation of Trichoderma fungi from coffee roots was done by incubating the sterilized coffee roots in a layer of moist filter paper in a Petri dish for seven days. Identification of Trichoderma by observing the characteristics of the colony on the medium of potato dextrose agar (PDA) and microscopic media using microcultures (slide culture). Inhibition of Trichoderma fungi against Colletotrichum was tested by multiple culture methods on PDA media. The results of root incubation in humid conditions showed that there was four morphospecies of Trichoderma fungi, each of which had different characteristic specifications. In vitro antagonism in test on PDA medium, the first three morphospecies against Colletotrichum showed that each Trichoderma could inhibit 70.2%, 65.8%, and 63.3%, respectively, five days after inoculation. This data shows that Trichoderma isolated from coffee roots from Alor has the potential to suppress the growth of anthracnose pathogens.
KEANEKARAGAMAN CENDAWAN RIZOSFER PADA TANAMAN TALAS SATOIMO Eka Wisdawati; Tutik Kuswinanti; Ade Rosmana; Andi Nasruddin
Agroplantae: Jurnal Ilmiah Terapan Budidaya dan Pengelolaan Tanaman Pertanian dan Perkebunan Vol 8 No 2 (2019): Agroplantae: Jurnal Ilmiah Terapan Budidaya dan Pengelolaan Tanaman Pertanian dan
Publisher : Jurusan Budidaya Tanaman Perkebunan, Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.197 KB) | DOI: 10.51978/agro.v8i2.86

Abstract

Rhizosphere is an ideal zone for microorganisms to grow and develop abundantly that influenced by plant roots exudates. Soil microorganisms have a symbiotic mutualism interaction with plants and their interaction influenced the availability of organic soil compound. Therefore, there is a need to investigate and analyze the diversity of fungi at the rhizosphere of economically important food crop like shutterstock taro potato (Satoimo). The study was conducted through several steps as follows: collection of soil samples, dilution of soil samples and isolation of fungi, identification and analyzing of variability of the isolates. The results showed that Satoimo rhizosphere had a diversity index 2.85 which was categorized as a modest index. This result indicated the stability of the fungi community at the Satoimo rhizosphere and its diversity as modest category. The diversity of the microorganisms for each soil sample indicate its organic compounds and as an important factor that determine quality and soil healthy status. Keywords: diversity, rhizosphere, fungi, organic matter
Microbial Isolation Derived from Pineapple Extract and Its Application on Cocoa Pod Rot Disease, Phytophthora palmivora Sylvia Sjam; Ade Rosmana; Danial Rahim; Vien Sartika Dewi; Untung Surapati
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 30 No 1 (2014)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v30i1.196

Abstract

Cocoa pod rot, caused by Phytophthora palmivora,is one of the most important diseases in cocoa farm of Indonesia. Yield losses due to the disease were estimated in the range of 33–99%. Pod rot disease could be controlled using antagonistic fungi, Trichodermasp. and Gliocladiumsp., isolated from pineapple extract. The objective of this study was to evaluate the effectiveness of the two microbes derived from pineapple extract in suppressing the cocoa pod rot disease. The research was conducted at the Plant Pathology Laboratory, Faculty of Agriculture, Hasanuddin University and Cocoa Plantation of Pattalassang II Farmers Group, Pattalassang Village, District of Bantaeng, South Sulawesi from May to September 2012. The results indicated that two species of antagonistic fungi, Trichodermasp. and Gliocladiumsp. were dominant in pineapples extract. The mixture of both of microbes showed the ability to suppress the development of pod rot disease on cocoa plantation. Key worlds: cocoa pod rod, Phytophthora palmivora, Trichoderma sp., Gliocladium sp.