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All Journal Jurnal Fitopatologi Indonesia Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Jurnal Biosilampari: Jurnal Biologi
Vinsen Willi Wardhana
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Agriculture, Biological Sciences & Forestry Environmental Science Medicine & Pharmacology

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Pathogenicity of Endophytic Fusarium oxysporum Isolated from Weeds in Banana Plantations against Bananas Seedlings var. Raja Bulu Vinsen Willi Wardhana; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 17 No 1 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.1.1-8

Abstract

Fusarium oxysporum merupakan penyebab penyakit layu dan banyak mematikan tanaman pisang. Cendawan ini diketahui mampu hidup dan bertahan sebagai endofit pada gulma dan menjadi sumber inokulum yang nantinya menyebabkan penyakit pada tanaman pisang. Penelitian ini dilakukan untuk mengisolasi F. oxysporum dari akar gulma yang tumbuh di sekitar pertanaman pisang di Bogor, Jawa Barat, kemudian menguji sifat patogenisitasnya. Identifikasi morfologi dilakukan dengan mengamati koloni, konidium, dan klamidosporanya. Uji patogenisitas isolat endofit dilakukan di laboratorium menggunakan bibit pisang var. Raja Bulu. Sebanyak 9 isolat F. oxysporum berhasil diisolasi dari gulma yang tumbuh di sekitar pertanaman pisang. Semua isolat menunjukkan ciri morfologi sebagai F. oxysporum, yaitu koloni berwarna ungu, makrokonidium berbentuk kano dengan tiga sekat false head pendek, mikrokonidium berbentuk silindris atau ginjal, dan klamidospora umumnya tersusun tunggal. Semua isolat menyebabkan insidensi penyakit sebesar 100% pada bibit pisang var. Raja Bulu dengan keparahan gejala layu pada daun antara 58.33% sampai 77.78% dan nekrosis pada bonggolnya antara 50.00% sampai 69.44%.
Analisis Molecular Docking Senyawa dari Jamur Endofit Bawang Dayak (Eleutherine bulbosa) Sebagai Inhibitor Lipase Pankreas Frans Grovy Naibaho; Ebrry Dwi Putra; Desimaria Panjaitan4; Vinsen Willi Wardhana
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (511.546 KB) | DOI: 10.33474/e-jbst.v7i2.484

Abstract

Nowadays some efforts are being made to reduce obesity by using drugs that aim to reduce appetite of the drug users. However, the use of drugs still has harmful side effects. Endophytic fungi originating from the Dayak onion plant are thought to have secondary metabolites that have potential as anti-obesity. The aims of this study were to determine the compounds produced by endophytic fungi of Dayak onion and to determine the potential components as inhibitors of pancreatic lipase enzymes. Samples of Dayak onions were collected from Palangka Raya using purposive sampling method. Isolation of endophytic fungi was conducted using surface sterilization method and the isolates were grown on PDA. Isolates were characterized and fermented on PDB for 14 days. The selected isolate was analyzed by using GCMS then molecular docking was performed using Autodock Vina and PyRx. The research results showed that there were 7 isolates of endophytic fungi isolated from leaves and roots tissues of Dayak onion. GCMS analysis showed that EBD1 contains 38 compounds. Molecular docking analysis showed that 1,3-Di-O-Acetylpentopyranose, a test ligand, has the closest binding affinity value (-6.6 kcal/mol) to orlistat (control) (-6.8 kcal/mol). Thus, it has the potential as an inhibitor of pancreatic lipase enzyme protein.
ANALISIS VEGETASI HABITAT NEPENTHES sp. DI HUTAN GAMBUT KAMPUS UNIVERSITAS PALANGKA RAYA, KALIMANTAN TENGAH Siti Sunariyati; Vinsen Willi Wardhana; Muhammad Rizki
Jurnal Biosilampari : Jurnal Biologi Vol 5 No 1 (2022): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v5i1.1849

Abstract

This research is a continuation of previous research on the diversity of pitcher plants (Nepenthes sp) at the University of Palangka Raya. This study aims to determine the habitat characteristics of the pitcher plant (Nepenthes sp) at the University of Palangka Raya. We carried out this research in June-September 2022. The habitat characteristics of pitcher plants were collected by purposive sampling to analyze habitat characteristics data using a quadratic sampling method consisting of two transects with 15 plots based on the findings of Nepenthes sp. at the research site. The first transect is in a secondary forest, and the second transect is in the scrub with slightly open conditions after the fire. The research area was dominated by tree species Cratoxylum glaucum, Acacia mangium, Melaleuca cajuputi, and understorey species dominated by Stenochlaena palustris. A diversity index value is classified as a medium for understorey and saplings and low for poles and trees.
Co-Authors Desimaria Panjaitan Ebrry Dwi Putra Frans Grovy Naibaho Muhammad Rizki Saiful Akhyar Lubis Siti Sunariyati SRI HENDRASTUTI HIDAYAT Suryo Wiyono