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Aplikasi Ribavirin Pada Shoot Tip Bawang Merah untuk Eliminasi Virus OYDV (Application of Ribavirin on Shoot Tip of Shallot for OYDV Eradication) Aqlima, nFN; Purwoko, Bambang Sapto; Hidayat, Sri Hendrastuti; Dinarti, Diny
Jurnal Hortikultura Vol 28, No 2 (2018): Desember 2018
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v28n2.2018.p251-258

Abstract

Kemoterapi merupakan aplikasi senyawa kimia (ribavirin) yang memiliki aktivitas antiviral guna menghambat ataupun menghentikan multiplikasi virus pada jaringan tanaman. Penelitian bertujuan mengetahui pengaruh konsentrasi ribavirin terhadap pertumbuhan shoot tip bawang merah untuk mengeliminasi virus OYDV. Penelitian dilaksanakan di Laboratorium Kultur Jaringan 3, Departemen AGH IPB dan Laboratorium Virologi Tumbuhan, Departemen Proteksi Tanaman IPB, sejak bulan Oktober 2015 hingga Juni 2016. Rancangan percobaan yang digunakan adalah kelompok lengkap teracak dengan dua faktor dan empat ulangan, setiap ulangan terdiri atas empat tabung kultur yang ditanam satu eksplan. Percobaan dilakukan secara terpisah pada dua kultivar bawang merah, yaitu Bima Brebes dan Tiron. Faktor pertama adalah konsentrasi ribavirin, yaitu 0, 5, 10, 15, dan 20 mg/L. Faktor kedua adalah ukuran eksplan (shoot tip), yaitu 1,1–2,0 mm dan 2,1–3,0 mm. Hasil penelitian menunjukkan bahwa peningkatan konsentrasi ribavirin menekan tinggi tunas cv. Bima Brebes, waktu muncul daun, tinggi tunas, dan jumlah daun cv. Tiron. Ukuran shoot tip yang lebih besar (2,1–3,0 mm) dapat meningkatkan persentase tumbuh eksplan dan mempercepat waktu muncul daun pada cv. Bima Brebes dan Tiron. Konsentrasi ribavirin yang diaplikasikan pada dua ukuran shoot tip masih belum dapat mengeliminasi virus OYDV pada kedua kultivar bawang merah.KeywordsBawang merah; In vitro; Ribavirin; Shoot tipAbstractChemotherapy is an apllication of chemistry compound (ribavirin) that has antiviral activity to inhibit virus multiplication in plant tissues. The objective of the experiment was to evaluate the effect of ribavirin concentrations on shoot tip growth of shallot for OYDV eradication. The experiment was cunducted at Tissue Culture Laboratory 3, Agronomy Department and Plant Virology Laboratory, Plant Protection Department of IPB from October 2015 until June 2016. The experiment was arranged in a completely rendomized block design with two factors and four replications. Each experimental unit consisted of four bottles with one explant in it. The first factor was ribavirin concentrations, i.e. 0, 5, 10, 15, and 20 mg/L. Second factor was shoot tip sizes, i.e. 1.1–2.0 mm and 2.1–3.0 mm. The experiment showed that increasing ribavirin concentrations suppressed the shoot length of cv. Bima Brebes, and it also inhibited the time of leaf to emerge, shoot length, and leaf number of cv. Tiron. Increasing shoot tip size (2.1–3.0 mm) influenced percentage of explant growth and speed the time of leaf emergance of cv. Bima Brebes and Tiron. Ribavirin concentrations used in this treatment did not eradicate OYDV in both shoot tipe sizes of the two cultivars.
TIGA SPESIES PERONOSCLEROSPORA PENYEBAB PENYAKIT BULAI JAGUNG DI INDONESIA Rustiani, Ummu S.; Sinaga, Meity S.; Hidayat, Sri Hendrastuti; Wiyono, Suryo
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1860

Abstract

Downy mildew is very detrimental disease of maize production in Indonesia. Adequate information regarding the identification key based on morphological and morphometric characteristic of the causal fungi of maize downy mildew in Indonesia is limited. Study for detection and identification of morphological, morphometric, and moleculer base is urgently required. Artificial sporulation induction method performed to obtain the morphology of the fungus as a whole.The fungi were morphologically identified as symptomatic maize downy mildew collected from 13 provinces in Indonesia.Three species, namely P. maydis, P.sorghi, and P. philippinensis were identified based on the shape,size, and conidial cell wall thickness, size and number of branching coniodiophores, and long sterigmata.Confirmation by PCR succesfully amplified target DNA of P. maydis, P. sorghi, and P. philippinensis. The identification key of third species of Peronosclerospora accurately to be used in identification of Peronosclerospora causes downy mildew of maize in Indonesia.This identification key is recommended as a method of identification of the causal downy mildew of maize in Indonesia.
Determination of Endophytic Fungi as Induce Resistance Agent of Chilli Pepper Against Pepper Yellow Leaf Curl Disease Lestari, Susanti Mugi; Hidayat, Sri Hendrastuti; Widodo, Widodo
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.989

Abstract

Pepper yellow leaf curl disease caused by Pepper yellow leaf curl virus becomes a major problem on chilli pepper in Indonesia since 2000. Endophytic fungi has the potency to suppress plant diseases by acting as induced resistant agent. The objective of this research was to determine the effect of endophytic fungi application on incidence of yellow leaf curl disease of chilli pepper. Four isolates of endophytic fungi, i.e. Cercospora nicotianae isolate H5, Curvularia sp. isolate H12, Fusarium sp. isolate AC-2.7 and AC-4.5 were applied as seed treatment and leaf spray on 2 chilli varieties, ‘Biola’ and ‘Luwes’. Observations of incubation period showed that delayed symptom occurred on application of Fusarium sp. isolate AC-2.7 and Curvularia sp. isolate H12. Disease incidence reached 100 % in all treatments. Less severe symptom was observed on application of Curvularia sp. isolate H12. Most plants infected by PYLCIV showed obvious symptoms of yellow mosaic, leaf curling and stunting. Plant productivity analysis showed that application of Fusarium sp. isolate AC-2.7 resulted higher fruit weight and this treatment may induce tolerant response of the plants to PYLCIV infection. Initial effort to evaluate endophytic fungi as biocontrol agents for pepper yellow leaf curl disease should be studied further.
INDUCED MUTATION BY GAMMA RAYS IRRADIATION TO INCREASE CHILLI RESISTANCE TO BEGOMOVIRUS Gaswanto, Redy; Syukur, Muhamad; Purwoko, Bambang Sapta; Hidayat, Sri Hendrastuti
AGRIVITA, Journal of Agricultural Science Vol 38, No 1 (2016): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i1.581

Abstract

Begomovirus infection has a significant impact of lowering chilli yield in Indonesia. A constraint of narrow genetic variability of chilli in Indonesia has made the mutation breeding program as a solution worth-pursuing in increasing the genetic variability. The objective of this study was to determine the LD50 point for each of the five irradiated chilli genotypes and the optimum dose of gamma irradiation in inducing chilli resistance to Begomovirus and other improved agronomical traits. The study was conducted in the Indonesian Vegetable Research Institute (IVEGRI) at Cikole-Lembang, elevation 1,200 m above sea level, from March to December 2013. Split plot design was used with genotype as main factor (Kencana, Lembang-1, SSP, Tanjung 2, Seloka) and irra-diation dosage as sub-factor (0, 200, 400, 600, 800 Gy). All treatments were replicated three times. The results showed that LD50 points of the five irradiated chilli genotypes were in the range of 422.64-629.68 Gy. There were some chilli genotypes in the population of M2 that had high coefficient variance genetic (CVG) and broad sense heritability (h2bs) value for disease incu-bation time. This could be used as resistance parameter to Begomovirus and improvement parameter of several agronomical traits.
Identifikasi Molekuler Begomovirus Penyebab Penyakit Kuning Keriting pada Tanaman Cabai (Capsicum annum L.) di Sumatera Barat Trisno, Jumsu; Hidayat, Sri Hendrastuti; Jamsari, Jamsari; Habazar, Trimuri; Manti, Ishak
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.888 KB) | DOI: 10.31258/jnat.13.1.41-46

Abstract

Pepper plants showing Begomovirus-like symtoms, consisting of yellowing, leaf curling, and distortion, werecollected from fields located in the Padang, West Sumatra. The aim of this research is to identification ofbegomovirus associated with yellow leaf curl diseases on pepper. Total DNA was extracted from infected leaftissue according to Doyle and Doyle (1999) with slight modification. Polymerase chain reaction (PCR) was used toamplify the coat protein region of the virus using universal degenerate primers pAV494 and pAC1048. The PCRamplified DNA product (approx. 560 bp) was sequenced. The nucleotide and amino acid sequences and BLASTsearch revealed highest homology with pepper yellow leaf curl Indonesia virus isolated pepper, tomato andAgeratum conyzoides from Java, but differences from those of tomato yellow leaf curl virus. The isolate was thententatively called pepper yellow leaf curl Indonesia virus-Padang (PYLCIV-Pdg).
PENGARUH INFEKSI VIRUS MOSAIK TERHADAP PRODUKSI DAN KADAR MINYAK TIGA VARIETAS NILAM Noveriza, Rita; Suastika, Gede; Hidayat, Sri Hendrastuti; Kartosuwondo, Utomo
Buletin Penelitian Tanaman Rempah dan Obat Vol 23, No 1 (2012): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v23n1.2012.%p

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Penyakit mosaik tercatat sebagai salah satu faktor pembatas dalam produksi tanaman nilam (Pogostemon cablin). Penelitian ini dilakukan untuk mengukur pengaruh infeksi virus penyebab penyakit mosaik terhadap produksi dan kadar minyak tanaman nilam. Bahan yang digunakan dalam penelitian ini adalah tiga varietas unggul tanaman nilam yaitu Sidikalang, Lhokseumawe dan Tapak Tuan. Infeksi Potyvirus berhasil dideteksi pada varietas Tapak Tuan dan Lhok-seumawe berdasarkan hasil metode ELISA. Pengukuran berat terna basah, terna kering, kadar minyak dan kadar patchouli alcohol (PA) yang dilakukan pada tanaman berumur enam bulan menunjukkan terjadinya penurunan produksi dan kadar minyak. Penurunan tertinggi berat terna basah, terna kering, kadar minyak dan kadar PA berturut-turut dapat mencapai 34,65, 40,42, 9,09 dan 5,06%.
Deteksi Virus Tungro pada Gulma Padi Sawah Menggunakan Teknik PCR Ladja, Fausiah T.; Hidayat, Sri Hendrastuti; Damayanti, Tri Asmira; Rauf, Aunu
Jurnal Penelitian Pertanian Tanaman Pangan Vol 35, No 1 (2016): April 2016
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.393 KB) | DOI: 10.21082/jpptp.v35n1.2016.p39-44

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Virus tungro disease is a serious problem to rice crop in a certain area of rice production in Indonesia. The disease is caused by a combined infection of Rice Tungro Bacilliform Virus (RTBV) and Rice Tungro Spherical Virus (RTSV). Both viruses were reported to infect ratoon rice plants, weeds, and wild rice. The study was conducted to detect RTBV and RTSV on some weeds. Weed samples were collected from rice fields in West Java, Bali, West Nusa Tenggara, Papua, and West Sumatera. The detection of RTBV and RTSV were carried out using Polymerase Chain Reaction (PCR) and Reverse Transcription (RT) – PCR, employing coat protein gene specific primers. RTBV specific DNA fragment of ~1400 bp size was successfully amplified from various weed species including: F. miliacea, C. iria, M. vaginalis, L. adscendens, S. zeylanica, D. sanguinalis, and E. crusgalli. RTSV specific DNA fragment of ~787 bp size was successfully amplified from weed species of F. miliacea, L. octovalvis, and D. sanguinalis. RTBV or RTSV specific DNA fragment was not amplified from L. flava and P. distichum. Weed samples infected by both viruses did not show any tungro symptom. Virus detection based on molecular technique was able to determine the status of weed whether it is as an alternate host of viruses. Weeds sanitation prior to rice planting, therefore, should be considered as an integral part of virus disease management.
Evaluation of Plant Growth Promoting Rhizobacteria as a Protecting Agent Against Cucumber Mosaic Virus and Chilli Veinal Mottle Virus on Chillipepper MUHAMMAD TAUFIK; SRI HENDRASTUTI HIDAYAT; GEDE SUASTIKA; SIENTJE MANDANG SUMARAW; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.974 KB) | DOI: 10.4308/hjb.12.4.139

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This study was conducted to evaluate the effectiveness of plant growth promoting rhizobacteria (PGPR) in protecting chillipepper plant from infection of cucumber mosaic virus (CMV) and chilli veinal mottle virus (ChiVMV). Seven isolates of PGPR, i.e. BC1, BTP2H, BTP3G, BTP3O BTP1, BTP2D, and T1F were applied as seed treatment and soil drench. Plants height, number of branch, and fruits weight were measured every one and ten weeks after virus inoculation. Virus concentration in plants and disease incidence were confirmed by enzyme-linked immunosorbant assay (ELISA). Results showed that inoculation with PGPR improved the seed germination. Eight days after sowing, the percentage of PGPR treated seed germination reached 50-84%; whereas those of untreated seed reached only 18%. In general, PGPR treatment significantly reduced (p < 0.05) the effect of virus infection on plant growth. Two PGPR isolates, i.e. BTP1 and BTP2H, maintained fruit weight of infected plants as good as those of healthy plants. Based on ELISA, PGPR was able to inhibit the disease incidence. The BTP3O and BTP2D isolates even protected the plant from ChiVMV infection. Concentration of salicylic acid and peroxidase were relatively higher on plants treated with PGPR than those without PGPR treatment. This gave an indication that PGPR may act as induction agents for systemic acquired resistance. Therefore, PGPR treatment is a promising strategy to control viral diseases on chillipepper.
Detection and Host Range Study of Virus Associated with Pepper Yellow Leaf Curl Disease SRI SULANDARI; RUSMILAH SUSENO; SRI HENDRASTUTI HIDAYAT; JUMANTO HARJOSUDARMO; SOEMARTONO SOSROMARSONO
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.61 KB) | DOI: 10.4308/hjb.13.1.1

Abstract

High incidence of Pepper yellow leaf curl virus (PepYLCV) was observed in Indonesia since early 2000. Disease incidence in Yogyakarta, Central and West Java reached 100% on Capsicum frutescens, but only 10-35% on C. annuum. As an exception, the disease incidence on C. annuum cv. TM 999 was in the range of 70-100%. The causal agent of the disease, PepYLCV, was detected by polymerase chain reaction. Viral specific DNA fragment of the size ~1600 bp and ~550 bp was amplified from infected plants using two pairs of geminivirus universal primers pAL1v1978/pAL1c715, and pAv494/pAc1048, respectively. The PepYLCV has an intermediate host range including plants belonging to the family of Solanaceae, Leguminosae, and Compositae. The species belonging to the families of Cucurbitaceae, Malvaceae, Chenopodiaceae, and Amaranthaceae were resistant to the virus. Physalis floridana, is very prospective as a propagation host for the geminivirus infecting pepper. Nicotiana spp., cucumber, watermelon, cotton, and Sida sp. could be used as a differential host. Besides, Capsicum frutescens cv. Cakra, tomato, N. benthamiana, N. glutinosa, and Ageratum conyzoides could be used as indicator plants for the geminivirus infecting pepper. Key words: Pepper yellow leaf curl virus, disease incidence, host range
Cucumber Mosaic Virus and Chili Veinal Mottle Virus Infection on Growth and Yield Component of Chilli DWI SUBEKTI; SRI HENDRASTUTI HIDAYAT; ENDANG NURHAYATI; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.44 KB) | DOI: 10.4308/hjb.13.2.53

Abstract

A research was undergone to study the effect of single and double infection of Cucumber Mosaic Virus (CMV) and Chili Veinal Mottle Virus (ChiVMV) on the growth and yield of five chilli cultivars, i.e. Prabu, Taro, Jatilaba, Laris, and Keriting Bogor. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA. Severe symptom was observed on plant given double infection compared to those given single infection. The rate of plant growth and the amount and weight of fruits were reduced. The type of interaction between CMV and ChiVMV on most chilli cultivar can be considered as interference and additive. Synergism interaction was only observed on cultivar Laris. Based on symptom expression and reduction on yield, it can be concluded that all chilli cultivars used in this study could not hold up the virus infection. Key words: CMV, ChiVMV, interaction, additive, interference, synergism
Co-Authors . SUDARSONO Abdul Muin Adnan ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT AUNU RAUF Ayu Kartini Parawansa Azmi Khoirin Nada Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Subekti DWI SUBEKTI Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Erika Rosminim Purba Evan P. Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Gede Suastika GEDE SUASTIKA Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika Giyanto Giyanto Hamdayanty Hamdayanty Hari Priwiratama Harwan Susetio Heri Harti Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro ISHAK MANTI Ishak Manti Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muhamad Syukur Muhammad Herman MUHAMMAD TAUFIK Muhammad Taufik Naimatul Farida Neni Gunaeni Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purnama Hidayat Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat Rahmi Yunianti Rahmi Yunianti Redy Gaswanto Redy Gaswanto, Redy Reymas M. R. Ruimassa Rina Rachmawati Rita Noveriza RITA NOVERIZA Rita Noveriza Rita Noveriza Rokhana Faizah Roy Ibrahim Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sakinah Inayatur Rizqiyah Sari Nurulita Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Sarsidi Sastrosumarjo Sherli Anggraini Sientje Mandang Sumaraw Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Suryo Wiyono Susanti Mugi Lestari Susanti Mugi Lestari Taufik, Muh Tega Kintasari Titiek Yulianti TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati Ummu S. Rustiani Ummu Salamah Rustiani UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Utomo Kartosuwondo Utomo Kartosuwondo Vinsen Willi Wardhana Wayan Winasa Widodo Widodo . Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Yudia Nurhaelena Zahratul Millah