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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Jurnal Silvikultur Tropika (JST) Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Bioplante Bumi Lestari Agrikultura Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Keteknikan Pertanian Jurnal Penelitian Hutan Tanaman Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Hexagro Jurnal Bioindustri (Journal of Bioindustry) Jurnal Penelitian Tanaman Industri (Littri) Jurnal Tanah dan Iklim Jurnal Risalah Kebijakan Pertanian dan Lingkungan Proceedings Series on Physical & Formal Sciences

Suryo Wiyono

Department Of Plant Protection, IPB University

Author-ID : 419707

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Social Sciences Veterinary

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PENGARUH PH DAN WAKTU PENYIMPANAN TERHADAP PERTUMBUHAN Lasiodiplodia theobromae PENYEBAB KANKER BATANG TANAMAN KARET Febbiyanti, Tri Rappani; Widodo, Widodo; Wiyono, Suryo; Yahya, Sudirman
Jurnal Penelitian Karet JPK : Volume 37, Nomor 1, Tahun 2019
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v37i1.615

Lasiodiplodia theobromae merupakan cendawan penyebab kanker batang pada tanaman karet (Hevea brasiliensis Muel Arg.), penyakit ini merupakan penyakit baru dan belum pernah dilaporkan sebelumnya di Indonesia. Banyak cara yang dapat dilakukan untuk mengendalikan penyakit kanker batang. Strategi pengendalian yang terbaik adalah dengan pengelolaan penyakit yang membutuhkan pengetahuan mengenai ekobiologi patogen, inang, dan interaksinya dengan lingkungan biotik dan abiotik. Pengendalian patogen harus didasarkan pada pengetahuan tentang bioekologinya. Diharapkan dengan pengetahuan bioekologi bisa dijadikan dasar untuk bisa menghambat perkembangan atau menurunkan populasi inokulum di lapangan. Penelitian ini bertujuan untuk menentukan pengaruh pH secara in vitro terhadap pertumbuhan patogen kanker batang dan laju penurunan viabilitas tubuh buah akibat penyimpanan. Kisaran pengaruh pH media meliputi 3, 4, 5, 6 dan 7. Penyimpanan tubuh buah dilakukan dalam wadah tanpa diberi tanah, diberi tanah steril dan tanah tidak steril. Penentuan viabilitas dilakukan pada 30, 60, 90 dan 120 hari masa penyimpanan. Semakin rendah pH menyebabkan pertumbuhan miselia terhambat.

TIGA SPESIES PERONOSCLEROSPORA PENYEBAB PENYAKIT BULAI JAGUNG DI INDONESIA Rustiani, Ummu S.; Sinaga, Meity S.; Hidayat, Sri Hendrastuti; Wiyono, Suryo
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1860

Downy mildew is very detrimental disease of maize production in Indonesia. Adequate information regarding the identification key based on morphological and morphometric characteristic of the causal fungi of maize downy mildew in Indonesia is limited. Study for detection and identification of morphological, morphometric, and moleculer base is urgently required. Artificial sporulation induction method performed to obtain the morphology of the fungus as a whole.The fungi were morphologically identified as symptomatic maize downy mildew collected from 13 provinces in Indonesia.Three species, namely P. maydis, P.sorghi, and P. philippinensis were identified based on the shape,size, and conidial cell wall thickness, size and number of branching coniodiophores, and long sterigmata.Confirmation by PCR succesfully amplified target DNA of P. maydis, P. sorghi, and P. philippinensis. The identification key of third species of Peronosclerospora accurately to be used in identification of Peronosclerospora causes downy mildew of maize in Indonesia.This identification key is recommended as a method of identification of the causal downy mildew of maize in Indonesia.

ORGANIC FERTILIZER AMENDMENTS REDUCE DISEASE SEVERITY OF Phytophthora palmivora ROOT ROT OF DUKU (Lansium domesticum) SEEDLING Hayati, Islah; Wiyono, Suryo; Widodo, Widodo; Sobir, Sobir
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 19 No. 2 (2019): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.219143-148

Phytophthora palmivora, is a deadly pathogen of duku, causing serious problem in the main production area, especially in Jambi Province, Indonesia. The pathogen can infect duku plant in all stage of growth including seedlings. Organic amended media might become a potential alternative option for this disease management. These studies were performed in soil media containing organic fertilizer of 0%, 10%, 20%, 30% (v/v), served as treatment, and subjected to completely randomized design with 5 replications. This research found that incorporating 20% of organic fertilizer into media was able to lower disease severity by 23%. The ability of organic fertilizer to increase media bacterial and maintain fungal density and increase chemicals of C-org, P, K, Ca, Mg and Zn played important roles in reducing disease development.

ANALYSIS OF CAUSATIVE FACTORS FOR CANKER DISEASE EPIDEMIC ON RUBBER PLANTS IN SOUTH SUMATRA Febbiyanti, Tri Rapani; Wiyono, Suryo; Yahya, Sudirman; Widodo, Widodo
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 19, No 1 (2019): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Analysis of Causative Factors for Canker Disease Epidemic on Rubber Plants in South Sumatra. Lasiodiplodia theobromae is the cause of stem canker in the rubber plant (Hevea brasiliensis), this is a new disease and has never been reported in Indonesia. Currently, data on environmental factors and cultivation techniques related to the development of stem canker are not available so research needs to be done on this subject. The objective of the study was to analyze environmental factors and cultivation techniques related to stem canker on rubber trees in southern Sumatra. The study was conducted by collecting data on environmental factors and cultivation techniques and then analyzed the association with stem canker. Field observation was carried out at 21 locations in PT. Perkebunan Nusantara VII Business Unit Padang Plawi Bengkulu, Tulung Buyut Lampung, Musilandas Palembang and Experimental Garden of Sembawa Research Center, Indonesian Rubber Research Institute at Palembang to measure the severity and incidence of this disease. Besides that, the data of cultivation technique and the condition of the garden were taken from the officer at those locations. Soil sample from each location was taken in a composite way, then its physical and chemical components were analyzed. The analysis was conducted to find out the correlation between cultivation and disease severity using chi-square test. The relationship between chemical and physical factors of the soil with the disease severity was analyzed using a multivariate test of principal component analysis/PCA. Regression analysis was conducted to show the possible relation to the incidence and severity of this disease. The location, clones, weed control and the number of plants per hectare showed a correlation with the disease severity. There was a positive correlation between incidence and severity of the disease. The highest disease severity occurred at the Sembawa location on clone BPM 24, with the number of plant population per ha was ? 555 trees and weed control using herbicide. Content of soil nitrogen, dust, clay and water as well as cation exchange capacity (CEC)were suggested to significantly contribute to the severity of stem canker.

Seleksi dan Identifikasi Bakteri Antagonis sebagai Agens Pengendali Hayati Penyakit Hawar Pelepah Padi Rustam, Rustam; Giyanto, Giyanto; Wiyono, Suryo; Santosa, Dwi Andreas; Susanto, Slamet
Jurnal Penelitian Pertanian Tanaman Pangan Vol 30, No 3 (2011): Desember 2011
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Selection and identification of effective microbes are important steps to obtain biological control agents. The objective of this research was to screen potential bacteria as controlling agents for rice sheath blight disease. The research was conducted at plant bacteriological laboratory and green house of Plant Protection Division of Bogor Agricultural University, Bogor, from May 2010 to February 2011. The trial was arranged in a completely randomized design with bacterial isolates as treatment. The result showed that 30 out of 144 bacterial isolates indicated an antifungal activity to R. solani. In vivo test indicated that 3 of the 30 isolates which have antifungal activity were able significantly to suppress the rice sheath blight disease. Those isolates were marked as TT47, SS19 and BR2, with the ability to suppress rice sheath blight disease at rate of 79.6, 56.4, and 49.4%, disease index 1.7, 3.7, and 4.3, and the disease incidence 33.3%, 73.35, and 80%, respectively. Molecular characterization of partial sequence of 16S rRNA on SS19, TT47, and BR2 isolates showed that those bacteria are Serratia marcescens, Ralstonia pickettii, and Bacillus subtilis, respectively.

Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi ARI SUSILOWATI; ARIS TRI WAHYUDI; YULIN LESTARI; ANTONIUS SUWANTO; SURYO WIYONO
HAYATI Journal of Biosciences Vol. 18 No. 2 (2011): June 2011
Publisher : Bogor Agricultural University, Indonesia

Plants are liable to be attacked by soilborne fungal pathogens which are responsible to reduce plant growth and losses in yield. In Indonesia, indigenous soybeans’ rhizobacteria such as antifungal producing Pseudomonas sp. have not many been reported yet. Therefore, the potential of the Pseudomonas sp. as biocontrol agent should be deeply explored. The aim of this study was to screen the indigenous soybeans’ rhizobacteria Pseudomonas sp. that possessing biocontrol characters against soilborne mainly i.e. Sclerotium rolfsii, Fusarium oxysporum, and Rhizoctonia solani, in vitro and in planta. Eleven isolates identified Pseudomonas sp. CRB numbered by CRB-3, CRB-16, CRB-17, CRB-31, CRB-44, CRB-75, CRB-80, CRB-86, CRB-102, CRB-109, and CRB-112 were affirmed to be candidates of biocontrol agents toward the soilborne fungal pathogens. Pseudomonas sp. CRB inhibited growth of the pathogenic fungi approximately 11.1-60.0% in vitro. Among of them, 7 isolates were also produced siderophore, 2 isolates produced chitinase, and 4 isolates produced hydrogen cyanide. Seed coating with the Pseudomonas sp. CRB accomplished disease suppression in planta about 14.3-100% in sterile soil condition and 5.2-52.6% in non sterile soil condition. Consistency in high performance more than 30% of disease suppression in non sterile soil condition suggested that 5 isolates i.e. CRB-16, CRB-44, CRB-86, CRB-102, and CRB-109 isolates have great promising to be developed as biocontrol agents of soilborne pathogenic fungi.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.2.66

Biological control of bacterial wilt pathogen (Ralstonia solanacearum) of tomato using endophytic bacteria is one of the alternative control methods to support sustainable agriculture. This study was conducted to select and characterize endophytic bacteria isolated from healthy tomato stems and to test their ability to promote plant growth and suppress bacterial wilt disease. Among 49 isolates successfully isolated, 41 were non-plant pathogenic. Green house test on six selected isolates based on antagonistic effect on R. solanacearum or ability to suppress R. solanacearum population in dual culture assays obtained BC4 and BL10 isolates as promising biocontrol agents. At six weeks after transplanting, plants treated with BC4 isolate showed significantly lower disease incidence (33%) than that of control (83%). Plants height was not significantly affected by endophytic bacterial treatments. Based on 16S rRNA sequence, BC4 isolate had 97% similarity with Staphylococcus epidermidis (accession number EU834240.1), while isolate BL10 had 98% similarity with Bacillus amyloliquefaciens strain JK-SD002 (accession number AB547229.1).

Biocontrol Activity, Mode of Action, and Colonization of Aureobasidium pullulans Dmg 30 DEP on Controlling Early Blight Disease on Tomato Plant Wawan Setiawan; Suryo Wiyono; Toga Pangihotan Napitupulu; Atit Kanti; Idris Idris; Masrukhin Masrukhin; Efi Toding Tondok; I Nyoman Sumerta; I Made Sudiana
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.320-329

We investigated the biocontrol activity of A. pullulans Dmg 30 DEP against Alternaria solani causal agent of early blight. Biocontrol activity was tested by the in vivo and ad planta. Biocontrol activity were tested by investigating the antibiosis capabilities with dual culture method, paper dish assay, two-compartment petri dish assay, and trapping and identification of volatile organic compounds (VOCs) with GC-MS. Lysis activity was examined by observing the clear zone formed by growing yeast on chitin agar and skim milk agar. The ability of hyperparasitism was assessed by the agar block method, and observed by light microscopy and scanning electron microscopy (SEM). The results showed that A. pullulans Dmg 30 DEP plays a role in the suppression of early blight disease at 106 cells/ml and 107 cells/ml yeast cell density. The mechanism involved in biocontrol activity is the production of VOCs, the production of chitinase and protease enzymes, the production of siderophore and hyperparasitism. The result shows that A. pullulans Dmg 30 DEP was colonizing the tomato leaves following the areole.

Pengaruh Seed Coating dengan Fungisida Benomil dan Tepung Curcuma terhadap Patogen Antraknosa Terbawa Benih dan Viabilitas Benih Cabai Besar (Capsicum annuum L.) Heny Setiyowati; Memen Surahman; Suryo Wiyono
Indonesian Journal of Agronomy Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this research was to study the effect of seed coating using Benomil and curcuma powder on seedborne diseases of antrachnose and seed viability of Capsicum annuum L.. This research was conducted in Laboratory of Plant Mycology and Laboratory of Seed Science and Technology IPB, from June to July 2006. This research consisted of three experiments, i.e.: (1) the effectiveness of seed coating in decreasing infection level of antrachnose fungus in the seed of chilli, (2) the effect of seed coating on seed viability, (3) the effect of seed coating in reducing infection level of antracnose at hypocotyl of chilli. The Experiment 1 and 3 used Randomized Complete Design and the experiment 2 used Randomized Complete Block Design. The result of this research showed that seed coating with Benomil and curcuma powder significantly decreased the infection level of antrachnose fungus on seed and hypocotyl (Benomil 2.5 g/l and curcuma powder 1 g/l). However, seed coating with curcuma 1 g/l had the lowest vigour index, seedling growth rate and seed germination (3%, 2.26%, 23%). Seedcoating with Benomil 2.5 g/l and curcuma powder did not significantly affect the maximum growth potential, seed germination, and vigour index. However, Benomil significantly decreased the seedling growth rate. Key wods : chilli, seed coating, antrachnose, curcuma, benomil, viability

Aktivitas Kitinase dan Peroksidase dari Ekstrak Protein Daun, Akar, Kalus dan Tunas In Vitro Trichosanthes tricuspidata Lour. Dewi Sukma; Roedhy Poerwanto; , Sudarsono; Nurul Khumaida; Suryo Wiyono; I Made Artika
Indonesian Journal of Agronomy Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

A number of Trichosanthes species has been reported as a source of bioactive protein associated with defense mechanisms such as chitinase. Chitinase and peroxidase of crude protein extracted from leaves, roots, in vitro calli and shoots of T. tricuspidata had been analysed. Calli were induced on MS medium containing combinations of 1 µM NAA + 1 µM BA (K1), 2 µM NAA + 2 µM BA (K2), 3 µM NAA + 3 µM BA (K3), or 4 µM NAA + 4 µM BA (K4). Shoots were cultured in MS with 1 mg/l of BA, while leaves and roots were harvested from six-month old plants grown on the field. Results of the experiment suggested that K1-K4 medium could be used to induce calli although weight of calli from all medium composition was not significantly different (0.19-0.31 g/explant/4 weeks). Calli from K1 medium had the highest of total crude protein content (3.24 mg/ml). The highest of chitinase activity was found in in vitro shoots (6.51 mM pNP/hour/mg protein) and the highest peroxidase activity was in the plant roots (0.25 ∆ 420/minute/mg protein). Key words: in vitro calli, shoots, crude protein, chitinase and peroxidase activities

Co-Authors . Giyanto . Rifka . Widodo . Widodo . Widodo ABDJAD ASIH NAWANGSIH Abdjad Asih Nawangsih Abdul Munif Agus Purwito Ahmad Fauzi Ridwan Ahmad Sutopo ALI NURMANSYAH Ali Wafa Aloysius Rusae Anas Dinurrohman Susila Andika Septiana Suryaningsih Andika Septiana Suryaningsih Andriani, Desta Anna Fariyanti Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R ARI SUSILOWATI Arif Ravi Wibowo Aris Tri Wahyudi Astika Widhi Pratiwi Atit Kanti Atit Kanti Awang Maharijaya Baskoro Sugeng Wibowo Bonny Poernomo Wahyu DADANG DADANG Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewi Sukma DWI ANDREAS SANTOSA Dyah Hariyanti Purnomo Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Efi Toding Tondok Elis Nina Herliyana Eryna Elfasari Rangkuti Evan P. Ramdan Evan Purnama Ramdan Evan Purnama Ramdan Fany Juliarti Panjaitan Febbiyanti, Tri Rapani Firman Hidayat Fitri Kemala Sandra Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Heny Setiyowati Heri Harti Heri Harti Hermanu Triwidodo I MADE ARTIKA I Made Sudiana I Made Sudiana I Nyoman Sumerta Ida Parida Idris Idris IKA DAMAYANTI Iskandar Z. Siregar Islah Hayati Istiaji, Bonjok Ita Aprilia Ivone Oley Sumarauw Juang Gema Kartika Kartini Budiastuti Ketty Suketi Ketty Suketi Khoirotul Afifah Kikin H Mutaqin Kristianto Nugroho Kristianto Nugroho Kudang Boro Seminar Kusuma Darma Kusuma Darma Lilik Retnowati Lina Herlina Lina Herlina Listihani, Listihani Masrukhin Masrukhin Mei Lita Fitriani Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Surahman Mutiara Dwi Lestari Napiudin Nazly Aswani Neo Endra Lelana nFN Khaerati Nika Ropiatningsuari Nur Alfi Saryanah Nurul Khumaida Phor Bho Ayuwati Priyo Cahyono Puji Lestari RAHAYU WIDYASTUTI Reflinur Reflinur Reflinur Reflinur Remaja Sitepu Rerenstradika T. Terryana Riana Jumawati Rika Estria Gurusinga Rizki Abi Amrullah ROEDHY POERWANTO Roza Yunita Rustam, Rustam Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Silviana Arsyad Sitti Eha Faihah Sitti Fadhilah Slamet Susanto Sobir Sobir Sobir Sobir Sri Hartati Sri Hartati Sri Hartati SRI HENDRASTUTI HIDAYAT stephanie stephanie Sudarsono SUDIRMAN YAHYA Suryahadi Suryahadi SUSILOWATI1 SUSILOWATI1 Suwarno . Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Thamrin Khamidi Titiek Siti Yuliani Toga Pangihotan Napitupulu Tondok, Efi Toding TRI ASMIRA DAMAYANTI Tri Rapani Febbiyanti, Tri Rapani Ummu S. Rustiani Ummu Salamah Rustiani Utami, Aditya Dyah UTUT WIDYASTUTI Vinsen Willi Wardhana Wartono Wartono Wawan Setiawan Wawan Setiawan Weni Wilia Widodo Widodo . Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widya Sari Winarso D. Widodo Winda Ika Susanti Yayu Siti Nurhasanah Yuli Fitriati Yuliawati YULIN LESTARI Yundari, Yundari Yuyun Andriyani

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