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All Journal Cropsaver : Journal of Plant Protection Al-Kimia Jurnal Ilmiah Berkala: Sains dan Terapan Kimia

Safri Ishmayana

Unknown Affiliation

Author-ID : 2470119

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

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Pembuatan Dan Aktivitas Antibakteri Yogurt Hasil Fermentasi Tiga Bakteri (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacilus acidophilus) Dian S. Kamara; Saadah D. Rachman; Ridya Widya Pasisca; Sadiah Djajasoepana; O. Suprijana; Idar Idar; Safri Ishmayana
Al-Kimia Vol 4 No 2 (2016): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Exploitation of synthetic antibiotics compounds not only have positive effect for human, but also have side effect that can be unfavorable, therefore many researches are being conducted to find natural antibiotics compounds that are safer. Lactic acid bacteria has the abilitytoproduce antibacterial compound when used in fermentation process.For example, Lactobacillus acidophilus produces acidophilin and acidolin. The main purpose of the present study is to investigate antibacterial activity of yogurt fermented with mixed bacterial culture of L. bulgaricus, S. thermophilus and L. acidophilus against Escherichia coli (representing Gram negative bacteria) and Bacillus subtilis (representing Gram positive bacteria). The antibacterial activity of the yogurt at three different time points (5, 7 and 9 hours) were examined. We also investigate the fermentation parameters of the yogurt production. The results of the present study indicate that the crude yogurt extract has antibacterial activity, where the highest activity was observed at 7 hour of incubation, resulting 0.35 and 0.30 cm of clear zone against E. coli and B. subtilis, respectively. It is most likely that the compound is non protein compound.

Resistance Level of Crocidolomia pavonana Against Profenofos Synthetic Insecticide and its Susceptibility To Azadirachta Indica Seed Extract Danar Dono; Yogas Dwi Pratiwi; Safri Ishmayana; Djoko Prijono
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

This research was conducted to know resistance level of Crocidolomia pavonana to a profenofos synthetic insecticide and to know susceptibility of C. pavonana to Azadirachta indica seed extract. The experiment using two bioassay methods, dry film method and feeding method. Field population of C. pavonana obtained from 3 villages in Lembang district i.e.Cibogo, Cikole and Kayu Ambon village. The standard insect has been maintenance more than 20 generations in the laboratory as a comparison. The result of the experiment indicated that population of C. pavonana form Cikole, Cibogo and Kayu Ambon village were resistant to profenofos insecticide with resistance ratio (RR) was low (1< RR ≤ 5). Resistance Ratio of C. pavonana from the third village were 4.69 and 4.83; 1.02 and 1.1 and 1.16 and 1.9 respectively (dry film and feeding method). The value of the resistance ratio was greater through the feeding assay compared to the contact assay. This indicated that the mechanism of C. pavonana resistance was likely to occur mainly in the digestive tract of insects. Enzime activity of the different sample population of C. pavonana indicated variation in their activity. This was indicated that every sample from different field could develop different mechanism of resistance. The toxicity study of A. indica seed extract to C. pavonana indicate that the field population of C. pavonana from the third village was susceptible to seed extract of A.indica with value RR of less than one point. Therefore seed extract of A. indica could be used as alternative control against C. pavonana which resistant to profenofos synthetic insecticide.Keywords: Resistance mechanism, Resistance ratio, Synthetic insecticide, Botanical insecticide

AN INSECTICIDAL COMPOUND FROM Barringtonia asiatica Rani Maharani; Safri Ishmayana; Yusuf Hidayat; Danar Dono
Jurnal Sains dan Terapan Kimia Vol 3, No 1 (2009)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Satu buah glikosida oleanan berhasil diisolasi dari biji Barringtonia asiatica. Struktur senyawa ini ditentukan oleh 1H- dan 13C-NMR satu dan dua dimensi serta oleh perbandingan langsung dengan standar. Senyawa ini menunjukkan aktivitas insektisida yang paling tinggi terhadap Crocidolomia pavonana. Dari penelitian ini diketahui bahwa biji B. asiatica mengandung senyawa insektisida yang paling aktif dengan nilai LC50 sebesar 290 ppm yang potensial untuk aplikasi insektisida.Kata Kunci: Barringtonia asiatica, glikosida oleanan, aktivitas insektisida, Crocidolomia pavonana

PRODUKSI POLI-Γ-ASAM GLUTAMAT DARI Bacillus subtilis B112 DENGAN VARIASI KONSENTRASI AMONIUM SUFAT SEBAGAI SUMBER NITROGEN DALAM MEDIA FERMENTASI Abubakar Sidik; Linar Z. Udin; Safri Ishmayana
Jurnal Sains dan Terapan Kimia Vol 5, No 1 (2011)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Poli-γ-asam glutamat (PGA) dan hasil degradasinya aman bagi manusia sehingga dapat dimanfaatkan sebagai bahan pengental, pelembab, pelepas berjangka atau sebagai pembawa obat. Meskipun banyak digunakan pada berbagai bidang industri, bahan ini masih diimpor dari luar negeri. Oleh karena itu, perlu dikembangkan suatu proses untuk memproduksi PGA secara efektif dan efisien. Penelitian ini dilakukan untuk menentukan konsentrasi optimum amonium sulfat sebagai sumber nitrogen untuk menghasilkan PGA dalam jumlah yang banyak. Produksi PGA dilakukan dengan fermentasi menggunakan bakteri Bacillus subtilis B112. Analisis yang dilakukan selama proses fermentasi meliputi pengukuran pH, derajat kekeruhan, berat kering sel dan viskositas media. Isolasi PGA dilakukan dengan tahapan sentrifugasi, pengendapan dengan metanol, dialisis dan liofilisasi. PGA yang telah diisolasi kemudian ditentukan berat molekulnya dengan meggunakan SDS-PAGE, sedangkan komposisi asam amino PGA ditentukan dengan menggunakan metode KLT. Hasil penelitian menunjukan bahwa konsentrasi ammonium sulfat yang optimum untuk menghasilkan PGA adalah 0,75% b/v. PGA yang diperoleh dengan variasi 0,75% amonium sulfat sebanyak 61,6 mg. Penentuan berat molekul dengan menggunakan SDS-PAGE menunjukan bahwa PGA yang diproduksi oleh B. Subtilis B112 memiliki berat molekul sekitar 205 kDa. Kata Kunci: Asam L-glutamat, amonium sulfat, B. Subtilis B112, PGA

Co-Authors Abubakar Sidik Danar Dono Dian S. Kamara DJOKO PRIJONO Idar Idar Linar Z. Udin O. Suprijana Rani Maharani Ridya Widya Pasisca Saadah D. Rachman Sadiah Djajasoepana Yogas Dwi Pratiwi Yusuf Hidayat

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