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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Agrikultura Jurnal Natur Indonesia Cropsaver : Journal of Plant Protection Agrovigor: Jurnal Agroekoteknologi Jurnal Penelitian Tanaman Industri Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroteknos Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri)

DJOKO PRIJONO

Departemen Proteksi Tanaman, Fakultas Pertanian Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680

Author-ID : 255063

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Industrial & Manufacturing Engineering Medicine & Pharmacology

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AKTIVITAS INSEKTISIDA EKSTRAK BUAH CABAI JAWA (Piper retrofractum) TERHADAP Helopeltis antonii (HEMIPTERA: MIRIDAE) INDRIATI, GUSTI; DADANG, DADANG; PRIJONO, DJOKO
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRAKHelopeltis antonii merupakan salah satu hama pada tanaman kakao,teh, dan jambu mete yang menyerang pucuk dan buah dengan menusukkanstiletnya untuk mengisap cairan sehingga menyebabkan kerusakan.Penelitian bertujuan menguji toksisitas ekstrak buah Piper retrofractum(cabai jawa) terhadap imago, pengaruh konsentrasi subletal terhadap nimfaketurunan, persistensi terhadap mortalitas dan oviposisi H. antonii.Penelitian dilaksanakan di Laboratorium Fisiologi dan ToksikologiSerangga, Institut Pertanian Bogor dan Laboratorium Proteksi Tanaman,Balai Penelitian Tanaman Industri dan Penyegar Sukabumi, mulai Mei2013 sampai April 2014. Buah mentimun digunakan sebagai inangpengganti untuk perbanyakan serangga uji H. antonii di laboratorium danmedia pengujian. Hasil penelitian menunjukkan kematian imago H.antonii, akibat perlakuan ekstrak P. retrofractum 0,05-0,3%, sudah terjadipada 24 jam setelah perlakuan (JSP). Sementara itu, pada 24 dan 48 JSPterjadi penurunan tingkat kematian H. antonii. Setelah 48 JSP, hanyaterjadi sedikit peningkatan kematian H. antonii. LC 50 dan LC 95 ekstrak P.retrofractum pada 120 JSP masing-masing 0,20 dan 0,49%. Jumlah nimfaketurunan yang dihasilkan pada perlakuan subletal ekstrak P. retrofractum0,203% (LC 50 ) lebih sedikit dibandingkan dengan konsentrasi 0,141%(LC 25 ). Ekstrak P. retrofractum yang dipajankan di bawah sinar mataharihingga 5 hari masih efektif terhadap imago (mortalitas 80%), tetapi tidakefektif dalam menghambat peletakan telur H. antonii. Penghambatanpeletakan telur terhadap imago H. antonii pada perlakuan ekstrak P.retrofractum 0,98% (2 Ã LC 95 ) yang dipajankan di bawah sinar matahariselama 0 dan 1 hari, dengan indeks penghambatan oviposisi 22,7 dan23,8%. Keefektifan ekstrak P. retrofractum perlu diuji di lapangan untukmenilai kelayakan dalam pengendalian H. antonii.Kata kunci: insektisida botani, mortalitas, oviposisi, persistensiABSTRACTHelopeltis antonii is cocoa, tea, and cashew nuts important pest thatcauses damage by sucking plant sap from shoots and nuts. This study wasconducted to test toxicity of Piper retrofractum fruit extract on adults,sublethal effect on the production nymphal progeny, and persistenceagainst mortality and oviposition of H. antonii. This study was conductedat The Fisiology and Insect Toxicology Laboratory, Plant ProtectionDepartement, Bogor Agricultural University and The Plant ProtectionLaboratory of Indonesian Industrial and Beverage Crops Research Institutefrom Mey 2013 to April 2014. Cucumber was used as a host substitute forrearing the test insect and as the testing medium. The results show thatadult mortality, due to the P. retrofractum leaf extract treatment 0.05-0.3%, has occured at 24 hours after treatment (HAT). Meanwhile, H.antonii mortality has decreased on 24 and 48 HAT. After 48 HAT, only aslight increased in H. antonii mortality. LC 50 and LC 95 of P. retrofractumextract at 120 HAT were 0.20 and 0.49%, respectively. The treatment atsublethal concentrations (LC 25 and LC 50 ) markedly decreased nymphalprogeny number. P. retrofractum extract suspensions at LC 95 and 2 Ã LC 95exposed under sunlight for 5 days were still effective against H. antoniiadults (80% mortality), but were not effective in females inhibitingoviposition. The oviposition inhibiting activity was observed only in thetreatment with P. retrofractum extract at 2 Ã LC 95Â exposed under sunlightfor 0 and 1 day in which the oviposition deterrency indices were 22.7 and23.8% respectively. Key words: botanical insecticides, mortality, oviposition, persistence

Potensi Insektisida Melur (Brucea javanica L. Merr) dalam Mengendalikan Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) dan Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) Lina, Eka Candra; Arneti, Arneti; Prijono, Djoko; Dadang, Dadang
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This study was conducted to evaluate the potency of Brucea javanica (melur) for controlling two species ofcrucifer pests, i.e. Crocidolomia pavonana and Plutella xylostella. Melur fruits, twigs, and leaves were extracteddirectly with methanol or sequentially with hexane, ethyl acetate, and methanol. The most active extract was thenfractionated by preparative layer chromatography using hexane, mixtures of ethyl acetate and methanol, andmethanol as eluents. The most active fraction was formulated as EC (emulsifiable concentrate) and WP (wettablepowder) formulations, and tested for their toxicity and antifeedant effect against C. pavonana and P. xylostellalarvae. The results showed that methanol extract of melur fruits was more active than that of twigs and leaves.Fractionation of methanol extract of melur fruits yielded an active fraction which was eluted with ethyl acetate-methanol 9:1. EC and WP formulations of melur fruits were active against C. pavonana larvae with LC50 of 0.39%and 0.21%, respectively. The same formulations were also active against P. xylostella larvae with LC50 of 0.31% and0.54%, respectively. In no-choice tests, the antifeedant effect of the EC formulation on C. pavonana larvae (feedinginhibition [FI]: 70.9%-97.5%) was higher than on P. xylostella larvae (FI: 52.2%-83.9%), but the antifeedant effect ofthe WP formulation on the two species was relatively the same. In a choice test, the EC formulation at LC 85completely inhibited feeding by C. pavonana larvae (FI: 100%).

AKTIVITAS INSEKTISIDA BAGIAN TUMBUHAN CALOPHYLLUM SOULTTRI BURM.F. (CLU IACEAE) TERHADAP LARVA LEPIDOPTERA Syahputra, Edy; Manuwoto, Syafrida; Darusman, Latifah K; ., Dadang; Prijono, Djoko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 4, No 1 (2004): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

The objective of this test was evaluate the insecticidal activity of ectract of some parts of Calophyllum soulattri (Clusiaceae) against larvae of three species of Lepidoptera, i. e Crocidolomia pavonana, Plutella xylostela, and Pieris sp.. Extraction of plant materials was done by infusion method using ethanol. The bioassays were conducted by leaf-feeding method. Second-instar larvae were fed extract-treated broccoli leaves of 48 hours, then they were presented with untreated leaves until the surviving larvae larvae reached the fourth-instar stage. The number of dead larvae was recorded daily an larval mortality date were analyzed by probit method. The result showed the gummy bark exudates and bark extract of old and young C. soulattri plants were highly active against C. pavonana. The abrk extact of old C. soulattri plant was also effective against P. xilostella and Pieris sp. The gummy exudates possessed strong insecticidal activity against C. pavonana larvae with LC50 of 0.04% and prolonged the developmental time from second to fourth instar of C. soulattri 2.03-7.25 days compared with control. The bark excudate gave positive respon to alkaloid flavonoid, and tannin test. Futher studies are needed to identify insecticidal compound in those active extracts.

PENGARUH CAMPURAN EKSTRAK AGLAIA HARMSIANA PERKINS DAN DYSOXYLUM ACUTANGULUM MIQ. (MELIACEAE) TERHADAP MORTALITAS DAN OVIPOSISI PLUTELLA XYLOSTELLA (L.) (LEPIDOPTERA: YPONOMEUTIDAE) Yuswanti, Lucy; Prijono, Djoko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 4, No 1 (2004): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Effect of Mixture of Aglaia harmsiana Perkins and Dysoxylum acuntangum Miq. (Meliaceae) Extracts on Mortality and Oviposition of Plutella xyostella (L.) (Lepidoptera: Yponomeutidae). This study was conducted to evaluate the effect of mixture of extracts of Aglaia harmsiana seeds and Dysoxylum acutangulum leaf petioles on mortality and oviposition of Plutella xyostella (L.) (Lepidoptera: Yponomeutidae). The insecticidal activity of ethyl acetate fraction of A. harmsiana and D. Acutangulum extracts and their mixture (concentration ratio of Ah and Da was 4:1) was evaluated against the thrid-instar P. xylostella was assessed by choice tests using broccoli seddlings. LC50 and LC95 of A. harmsiana extracts against the thirds the+fourth instar larvae were 0.054% and 0.129%, respectively, those of D. acutangulum extract were 0.031% and 0.103%, and those of the the mixture of A. harmsiana and D.acutangulum extract and the mixture at concentrations of 0.06% - 0.38%, 0.3%, and 0.02% - 0.3%, respectively, significantly reduced the number of eggs deposited by the female, P. xylostella on broccoli seedings, with oviposition inhibition indices of 0.30-0.59, 0.28-0.45 and 0.045-0.70. A.harmsiana ectract at all concentrations tested was phytotoxic to broccoli seedlings, whereas D. acutangulum extract was not. Mixture of A. harmsiana and D. acutangulum extract were phytotoxic to broccoli seedlings at concentrations of 0.06-0.3%. Thus, A. hamarsiana extract of mixture containing the extract should be used with caution and their phytotoxicity should be specifically tested on target crops before being used in the field.

INSECTICIDAL JOINT ACTION OF TERNARY EXTRACT MIXTURES OF FOUR SPECIES OF TROPICAL PLANTS AGAINST Plutella xylostella AND Crocidolomia pavonana Prijono, Djoko; Anggraini, Maya; Maryani, Sri Riski; Surya, Eliana Diana
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12134-42

Insecticidal joint action of ternary extract mixtures of four species of tropical plants against Plutella xylostella andCrocidolomia pavonana. The diamondback moth (DBM), Plutella xylostella, and the cabbage head caterpillar (CHC),Crocidolomia pavonana, were two main pests of cruciferous vegetable crops. Among the viable alternative ingredients thatcould be utilized to control those two pests were eco-friendly botanical insecticides. This study was conducted to evaluatethe joint action of ternary extract mixtures of four tropical plant species, i.e. Piper aduncum (Pa), Piper retrofractum (Pr),Sapindus rarak (Sr), and Tephrosia vogelii (Tv), against DBM and CHC. Results of leaf-residue feeding bioassays showedthat based on co-toxicity ratio at both LC 50 and LC levels, ternary mixtures of Pa extract with Sr and Tv extracts at concentrationratios of 1:5:1, 1:5:2, and 2:5:1 indicated synergistic joint action on DBM larvae. A mixture of Pa, Sr and Tv extracts at a ratio of2:5:1 was also synergistic to CHC at both LC level but antagonistic at the LC 95 50 95 and LC 95 levels. This mixture at the 1:5:1 ratio was synergistic on CHC at the LC level whereas at the 1:5:2 ratio was antagonistic to CHC at both LC levels. Furthermore, ternary mixtures of Pr extract with Sr and Tv extracts at the three concentration ratios were synergistic to CHC. Thus, ternary mixtures of Pa or Pr extract with Sr and Tv extracts at appropriate concentration ratios are potential alternatives for the control of DBM and CHC.

Physiological Response of Crocidolomia pavonana to the Calophyllum soulattri Active Fraction EDY SYAHPUTRA; DJOKO PRIJONO; DADANG DADANG; SYAFRIDA MANUWOTO; LATIFAH KOSIM DARUSMAN
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

The objective of this study was to evaluate the physiological response of the cabbage head caterpillar Crocidolomia pavonana treated with an active fraction of Calophyllum soulattri bark extract. Extraction of the test plant materials were performed with maceration method using methanol, continued by counter-current distribution separation in ethylacetate and water. Methanol fractionation of C. soulattri was performed by vaccuum liquid chromatography and the bioassays were conducted by a leaf-feeding method. The results showed that the dichloromethane fraction of C. soulattri had strong insecticidal activity against C. pavonana larvae, with LC50 of 0.05%. Sublethal treatments with the active fraction at LC15, LC50, and LC85 reduced the relative growth rate of the fourth instars by 48.9-94.1%. The treatments with the fraction at LC15 and LC50 to the fourth instars reduced the activity of invertase and protease enzyme by 20.7-24.1 and 14.4-25.14%, respectively, but increased the activity of trehalase by 26.7-120% as compared with controls. Key words: Physiological response, Crocidolomia pavonana, Calophyllum soulattri, botanical insecticide

Pengembangan teknologi formulasi insektisida nabati untuk pengendalian hama sayuran dalam upaya menghasilkan produk sayuran sehat . Dadang; Djoko Prijono
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The use of synthetic insecticide for pest management to protect insect attacking the holticulture product have been done intensivelly and seems not wise which predicted could cause a negative impact to the environmental and human health. Due to that reason then the research prepared to know the pest management technology especially for holticultural product through the use of material or something that come from the agricultural planting material as natural insecticide which could be developed as commercial products that practice and safe to produce healty holticultural product. Three species of fruits and holticultural plant used for the research namely Annona squamosa (Annonaceae/ seed), Piper retrofractum (Piperaceae/fruit) and Tephrosia vogelii (Leguminosae/Leaves) extracted with methanol. Every extract product tested with larva of Cricidolomia pavonana F. (Lepidoptera : Crambidae), that is one of the pest for holticultural product which treated with direction the planting and in the leaves. The extract was tested also to the food barrier of C. Pavonana. The extract of A. Squamosa was contact toxic than abdomen toxic, while on the contrary the extract of P. retrofractum have more impart to the abdomen toxic than contact toxic. The extract of P. Retrofractum and A. Squamosa at the concertation of 0.2 °/o could protect food activities of the larvas that was about 80 °/o. The mixed extract of T. Voge/ii and A. Squamosa more toxic or more effective than the mixed extract of T. Vogelii and P. Retrofractum. In the developing natural insecticide formula, the using of agristic adjuvant was better than tween and miracle especially in formulation establishization. The treatment of P. Retrofractum and T. Vogelii in the field could reduce the development of C. Pavonana which finally those both extract could be used wider as combined with A. Squamosa extract in order to increase the effectiveness.

Pengendalian Penyakit Busuk Pangkal Batang Lada dengan Ekstrak Pinang, Gambir, Sirih, dan Kapur Sirih Dedek Kusvianti; Widodo Widodo; Djoko Prijono
Jurnal Fitopatologi Indonesia Vol 10 No 4 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Pepper is an important commodity in Indonesia due to its export value. Infection of Phytophthora capsici causing stem rot disease is very critical for the production of pepper plants. Research was conducted to evaluate the efficiency of extracts of areca, gambir, betle, and lime paste for controlling stem rot disease. In vitro and in vivo experiments was designed to evaluate 2 factors, i.e. type and concentration of extracts. In vitro experiment was conducted by growing 4 day old and 5 mm in diameter of P. capsici isolate on V8 media containing the extracts tested. In vivo experiment was done by dipping pepper leaves on the extracts, followed by dropping zoospores of P. capsici on underneath of leaf surface. Mixture of 4 extracts (areca + gambir + betel + lime paste) of 0.005% and 0.01%; and areca extract of 0.04% was able to inhibit the growth of P. capsici colonies effectively in vitro. Similarly, mixture of 4 extracts of 0.01% effectively suppressed disease severity in vivo with equal effect with those of propineb fungicide of 0.2%.

Resistance Level of Crocidolomia pavonana Against Profenofos Synthetic Insecticide and its Susceptibility To Azadirachta Indica Seed Extract Danar Dono; Yogas Dwi Pratiwi; Safri Ishmayana; Djoko Prijono
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

This research was conducted to know resistance level of Crocidolomia pavonana to a profenofos synthetic insecticide and to know susceptibility of C. pavonana to Azadirachta indica seed extract. The experiment using two bioassay methods, dry film method and feeding method. Field population of C. pavonana obtained from 3 villages in Lembang district i.e.Cibogo, Cikole and Kayu Ambon village. The standard insect has been maintenance more than 20 generations in the laboratory as a comparison. The result of the experiment indicated that population of C. pavonana form Cikole, Cibogo and Kayu Ambon village were resistant to profenofos insecticide with resistance ratio (RR) was low (1< RR ≤ 5). Resistance Ratio of C. pavonana from the third village were 4.69 and 4.83; 1.02 and 1.1 and 1.16 and 1.9 respectively (dry film and feeding method). The value of the resistance ratio was greater through the feeding assay compared to the contact assay. This indicated that the mechanism of C. pavonana resistance was likely to occur mainly in the digestive tract of insects. Enzime activity of the different sample population of C. pavonana indicated variation in their activity. This was indicated that every sample from different field could develop different mechanism of resistance. The toxicity study of A. indica seed extract to C. pavonana indicate that the field population of C. pavonana from the third village was susceptible to seed extract of A.indica with value RR of less than one point. Therefore seed extract of A. indica could be used as alternative control against C. pavonana which resistant to profenofos synthetic insecticide.Keywords: Resistance mechanism, Resistance ratio, Synthetic insecticide, Botanical insecticide

Synergistic Activity of Mixtures of Piper aduncum Fruit Extract and Three Microorganism-Derived Insecticides against the Diamond Back Moth, Plutella xylostella Wanda Russianzi; Djoko Prijono
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Plutella xylostella is an important pest of Brassicaceae vegetable crops. Frequent use of insecticides containing single active ingredients can cause resistance in P. xylostella. This work was done to evaluate the synergism between Piper aduncum fruit extract and three microorganism-derived insecticides, i.e. abamectin, chlorfenapyr, and spinetoram, against P. xylostella from Pacet District, Cianjur Regency. Results of leaf-residue feeding bioassays with 48-h feeding treatment showed that LC95 of abamectin, chlorfenapyr, and spinetoram at 96 h after treatment (HAT) was about 5.8, 7.0, and 1.9-fold higher than their respective field rates. Thus, based on leaf-feeding assays P. xylostella larvae from Pacet-Cianjur were not susceptible to abamectin and chlorfenapyr, but were still fairly susceptible to spinetoram. LC95 of P. aduncum extract at 96 HAT was 0.68% (w/v). Based on combination index at the LC95 level - 96 HAT, mixtures of P. aduncum extract with all three test insecticides were synergistic. Thus, P. aduncum extract is potential to be used as an alternative ingredient to increase the effectiveness of the three test insecticides against P. xylostella.Keywords: Botanical insecticides, cabbage pest, insecticide mixtures, microorganism-derived insecticides, synergism.

Co-Authors . Dadang Abdurakhman Abdurakhman Ade Syahputra Agustin Zarkani Ahmad Sifa Arneti Arneti AUNU RAUF Bambang W. Nugroho Dadang . DADANG DADANG DADANG DADANG DADANG DADANG DAMAYANTI BUCHORI DANAR DONO Danar Dono Dedek Kusvianti Edy Syahputra EDY SYAHPUTRA EDY SYAHPUTRA Edy Syahputra Eka Candra Lina Eka Chandra Lina Erwin Cuk Surahmat Ferdi Ferdi Gracia Mediana GUSTI INDRIATI Gusti Indriati IDAR IDAR IKHA MUSLIKHA Indah Arastuti Nasution Istiaji, Bonjok Joni Hidayat latifah K Darusman Latifah K Darusman Latifah Kosim Darusman Maryani, Sri Riski Maya Anggraini Muhammad Sigit Susanto Mustopha Ahad Nur Alfi Saryanah Partomuan Simanjuntak PURNAMA HIDAYAT R. Dewi Ratna Wulan Safri Ishmayana Shinta Puspitasari Sudarmo Sudarmo Surya, Eliana Diana SYAFRI ISMAYANA SYAFRIDA MANUWOTO Syafrida Manuwoto SYAFRIDA MANUWOTO Wanda Russianzi Widodo Widodo Yan Yanuar Syahroni Yogas Dwi Pratiwi Yuswanti, Lucy

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