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Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan Purnama Hidayat; Djoko Prijono
Jurnal Entomologi Indonesia Vol 3 No 1 (2006): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.993 KB) | DOI: 10.5994/jei.3.1.50

Abstract

Soil samples were collected from each plot at the two field sites at 0, 1, 3, and 5 months after planting, and at one-month intervals after harvest for three months. Soil suspension from each sample was mixed with agar based diet (1 g soil/20 ml soil-diet mixture) then was presented to neonate larvae of H. armigera. Two reference standards were used, i.e. pure Cry1Ac protein (0.165 x 104 to 1.436 x 105 ng per ml of the proteindiet mixture) and non-Bt-soil spiked with Cry1Ac protein (0.11 x 104 to 9.573 x 104 ng per ml of the soil-diet-mixture). Larval mortality was recorded at 7 days of treatment. In both reference standards, mortality of H. armigera larvae occurred in a concentrationdependent fashion, i.e. larval mortality increased with the increase in Cry1Ac protein concentration, with LC50 of 0.95 x 104 and 1.65 x 104 ng/ml, respectively, for the pure protein and spiked non-Bt-soil standards. Result of the bioassay of soil samples from the field sites showed the residues from both transgenic cotton soils did not cause any mortality (0%) in the test insects. This indicates that the concentrations of Cry1Ac residue in soil at the study sites were very low (in the second standard, Cry1Ac at a concentration of 0.33 x 104 ng/ml caused about 10% larval mortality).
Keberhasilan hidup parasitoid Diadegma semiclausum Hellen dan serangga inangnya Plutella xylostella (L.) terhadap aplikasi ekstrak biji srikaya (Annona squamosa L.) Bonjok Istiaji; Djoko Prijono; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 15 No 1 (2018): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.621 KB) | DOI: 10.5994/jei.15.1.10

Abstract

Control of Plutella xylostella L. can be done both biologically and chemically. Biological control is generally done by utilizing plant extracts to kill insect pests. This study aims to determine the effect of the extract of seeds on the mortality and development of parasitoid Diadegma semiclausum Hellen and its host, P. xylostella. The effect of Srikaya seed extract on P. xylostella larvae in the initial stage was tested by foliar rest method, while the residue method on the glass surface was used to test the effect of the extract of seed extract of Srikaya on the death of imago D. semiclausum. The effect of seed extract on parasitization and development of D. semiclausum was performed by exposing P. xylostella larvae contaminated by the extract of srikaya seeds in sublethal concentration (LC5 and LC10) in imago D. semiclausum. The results showed that extract of srikaya seeds at a concentration of 0.0632–0.1% at 24-hour contamination affected the death of the larva P. xylostella and the imago parasitoids D. semiclausum. Imago parasitoids are more susceptible to increased concentration of extracts than P. xylostella larvae. The developmental resistance of P. xylostella by Srikaya seed extract at concentrations used is generally unreal. Similarly, the barriers to the development of parasitoid D. semiclausum in hosts contaminated with sublethal concentrations (LC5 and LC10) of seed extract are generally not significantly different. The presence of Srikaya seed extract in the host also has no significant effect on the extent of parasitization, cocoon length, cocoon width, and cocoon weight. Therefore, there is a possibility to chemically and biologically integrate P. xylostella control.
PENGARUH FRAKSI AKTIF KULIT BATANG DYSOXYLUM ACUTANGULUM MIQ. (MELIACEAE) TERHADAP REPRODUKSI CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) Edy Syahputra, Djoko Prijono, dan Partomuan Simanjuntak .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 2 No. 1 (2002): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.454 KB) | DOI: 10.23960/j.hptt.121-7

Abstract

Effects of an active fraction of stem bark of Dysoxylum acutangulum Miq. (Meliaceae) on reproduction of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae). This study was conducted to determine the effect of an active fraction of Dysoxylum acutangulum stem bark on reproduction and longevity of the cabbage pest Crocidolomia pavonana. The feeding treatment with the test material was given to the second-instar larvae C. pavonana at concentrations of 2.54, 3.29 and 3.93 ppm (equivalent to LC10, LC30 and LC50), then the emerging adults were observed for their fecundity, ovarial protein content and longevity. The treatment with the test material at LC50 shortened the lifespan of adult males and females C. pavonana by 2.9 and 1.6 days, respectively, compared with controls. The treatments at LC10, LC30 and LC50 decreased the fecundity of the adult females by 44.0, 64.7 and 58.1%, respectively, compared with controls, and this effect could be associated with the decrease in protein content of ovaries of the females (29.0, 47.8 and 42.8% decrease, respectively). Such treatments also delayed the maturity of ovaries of the females. Suppression of reproductive capacity of C. pavonana could contribute significantly to overall suppression of the pest population if the test material is used in the field.
Potensi Insektisida Melur (Brucea javanica L. Merr) dalam Mengendalikan Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) dan Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) Lina, Eka Candra; Arneti, Arneti; Prijono, Djoko; Dadang, Dadang
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.412 KB) | DOI: 10.31258/jnat.12.2.109-116

Abstract

This study was conducted to evaluate the potency of Brucea javanica (melur) for controlling two species ofcrucifer pests, i.e. Crocidolomia pavonana and Plutella xylostella. Melur fruits, twigs, and leaves were extracteddirectly with methanol or sequentially with hexane, ethyl acetate, and methanol. The most active extract was thenfractionated by preparative layer chromatography using hexane, mixtures of ethyl acetate and methanol, andmethanol as eluents. The most active fraction was formulated as EC (emulsifiable concentrate) and WP (wettablepowder) formulations, and tested for their toxicity and antifeedant effect against C. pavonana and P. xylostellalarvae. The results showed that methanol extract of melur fruits was more active than that of twigs and leaves.Fractionation of methanol extract of melur fruits yielded an active fraction which was eluted with ethyl acetate-methanol 9:1. EC and WP formulations of melur fruits were active against C. pavonana larvae with LC50 of 0.39%and 0.21%, respectively. The same formulations were also active against P. xylostella larvae with LC50 of 0.31% and0.54%, respectively. In no-choice tests, the antifeedant effect of the EC formulation on C. pavonana larvae (feedinginhibition [FI]: 70.9%-97.5%) was higher than on P. xylostella larvae (FI: 52.2%-83.9%), but the antifeedant effect ofthe WP formulation on the two species was relatively the same. In a choice test, the EC formulation at LC 85completely inhibited feeding by C. pavonana larvae (FI: 100%).
AKTIVITAS INSEKTISIDA EKSTRAK BUAH CABAI JAWA (Piper retrofractum) TERHADAP Helopeltis antonii (HEMIPTERA: MIRIDAE) PRIJONO, DJOKO; DADANG, DADANG; INDRIATI, GUSTI
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

ABSTRAKHelopeltis antonii merupakan salah satu hama pada tanaman kakao,teh, dan jambu mete yang menyerang pucuk dan buah dengan menusukkanstiletnya untuk mengisap cairan sehingga menyebabkan kerusakan.Penelitian bertujuan menguji toksisitas ekstrak buah Piper retrofractum(cabai jawa) terhadap imago, pengaruh konsentrasi subletal terhadap nimfaketurunan, persistensi terhadap mortalitas dan oviposisi H. antonii.Penelitian dilaksanakan di Laboratorium Fisiologi dan ToksikologiSerangga, Institut Pertanian Bogor dan Laboratorium Proteksi Tanaman,Balai Penelitian Tanaman Industri dan Penyegar Sukabumi, mulai Mei2013 sampai April 2014. Buah mentimun digunakan sebagai inangpengganti untuk perbanyakan serangga uji H. antonii di laboratorium danmedia pengujian. Hasil penelitian menunjukkan kematian imago H.antonii, akibat perlakuan ekstrak P. retrofractum 0,05-0,3%, sudah terjadipada 24 jam setelah perlakuan (JSP). Sementara itu, pada 24 dan 48 JSPterjadi penurunan tingkat kematian H. antonii. Setelah 48 JSP, hanyaterjadi sedikit peningkatan kematian H. antonii. LC 50 dan LC 95 ekstrak P.retrofractum pada 120 JSP masing-masing 0,20 dan 0,49%. Jumlah nimfaketurunan yang dihasilkan pada perlakuan subletal ekstrak P. retrofractum0,203% (LC 50 ) lebih sedikit dibandingkan dengan konsentrasi 0,141%(LC 25 ). Ekstrak P. retrofractum yang dipajankan di bawah sinar mataharihingga 5 hari masih efektif terhadap imago (mortalitas 80%), tetapi tidakefektif dalam menghambat peletakan telur H. antonii. Penghambatanpeletakan telur terhadap imago H. antonii pada perlakuan ekstrak P.retrofractum 0,98% (2 × LC 95 ) yang dipajankan di bawah sinar matahariselama 0 dan 1 hari, dengan indeks penghambatan oviposisi 22,7 dan23,8%. Keefektifan ekstrak P. retrofractum perlu diuji di lapangan untukmenilai kelayakan dalam pengendalian H. antonii.Kata kunci: insektisida botani, mortalitas, oviposisi, persistensiABSTRACTHelopeltis antonii is cocoa, tea, and cashew nuts important pest thatcauses damage by sucking plant sap from shoots and nuts. This study wasconducted to test toxicity of Piper retrofractum fruit extract on adults,sublethal effect on the production nymphal progeny, and persistenceagainst mortality and oviposition of H. antonii. This study was conductedat The Fisiology and Insect Toxicology Laboratory, Plant ProtectionDepartement, Bogor Agricultural University and The Plant ProtectionLaboratory of Indonesian Industrial and Beverage Crops Research Institutefrom Mey 2013 to April 2014. Cucumber was used as a host substitute forrearing the test insect and as the testing medium. The results show thatadult mortality, due to the P. retrofractum leaf extract treatment 0.05-0.3%, has occured at 24 hours after treatment (HAT). Meanwhile, H.antonii mortality has decreased on 24 and 48 HAT. After 48 HAT, only aslight increased in H. antonii mortality. LC 50 and LC 95 of P. retrofractumextract at 120 HAT were 0.20 and 0.49%, respectively. The treatment atsublethal concentrations (LC 25 and LC 50 ) markedly decreased nymphalprogeny number. P. retrofractum extract suspensions at LC 95 and 2 × LC 95exposed under sunlight for 5 days were still effective against H. antoniiadults (80% mortality), but were not effective in females inhibitingoviposition. The oviposition inhibiting activity was observed only in thetreatment with P. retrofractum extract at 2 × LC 95  exposed under sunlightfor 0 and 1 day in which the oviposition deterrency indices were 22.7 and23.8% respectively. Key words: botanical insecticides, mortality, oviposition, persistence
KEEFEKTIFAN TIGA JENIS INSEKTISIDA NABATI TERHADAP KUTU PUTIH PEPAYA PARACOCCUS MARGINATUS DAN KEAMANANNYA TERHADAP LARVA KUMBANG PREDATOR CURINUS COERULEUS Ahmad Sifa; Djoko Prijono; Aunu Rauf
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.702 KB) | DOI: 10.23960/j.hptt.213124-132

Abstract

Effectiveness of three botanical insecticides against the papaya mealybug Paracoccus marginatus and their safety to the predatory Curinus coeruleus larvae. Preparations of Tephrosia vogelii (Tv) leaf extract (0.5% and 1% w/v), Annona squamosa (As) seed extract (0.5% and 1%), and Cinnamomum multiforum (Cm) leaf essential oil (1% and 2%), and their mixtures (Mix-1: Tv 0.25% + As 0.25% + Cm 0.5%; Mix-2: Tv 0.5% + As 0.5% + Cm 1%) were tested for their effectiveness on third-instar nymphs of Paracoccus marginatus by spraying the test materials on undetached papaya leaves and by direct spraying on the test insects using Potter spray tower. Tv extract was also applied on the test insects placed on undetached papaya leaves. The safety test was done by direct spraying of the test materials on the predatory Curinus coeruleus larvae using Potter spray tower. Tv and As extracts at a concentration of 1% each are potential to be used for the control of P. marginatus. Spraying of T. vogelii extract on the test P. marginatus nymphs placed on papaya leaves was more effective than spraying of the test materials on papaya leaves or direct spraying on the test insects only. The treatment with Cm essential oil required twice the concentration of Tv and As extracts to obtain the same level of effectiveness. The treatment with Mix-2 caused lower P. marginatus mortality than the sum of mortality caused by its components applied separately. Nonetheless, the three botanical insecticides and their mixtures were safe to C. coeruleus larvae. On the other hand, although the synthetic insecticide imidacloprid (neonicotinoid), included in the study for comparison, was effective against P. marginatus, it was also toxic to the predatory C. coeruleus larvae so its use should be avoided or limited.
SEDIAAN INSEKTISIDA CALOPHYLLUM SOULATTRI: AKTIVITAS INSEKTISIDA DAN RESIDU TERHADAP LARVA CROCIDOLOMIA PAVONANA DAN KEAMANAN PADA TANAMAN Edy Syahputra, Djoko Prijono, .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 1 (2007): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.506 KB) | DOI: 10.23960/j.hptt.17%p

Abstract

Insecticide Preparation of Calophyllum soulattri: Insecticidal and Residual Activity Against Crocidolomia pavonana and Its Savety on Crops. Bioassays were done using leaf-residual method with Crocidolomia pavonana larvae as test insect. The number of dead larvae was recorded daily and was analyzed using probit method. The results showed that methanol extract, and dichloromethane fraction of C. soulattri bark possessed strong lethal effect against C. pavonana larvae with LC50 of 0.15%, and 0.05%, respectively. Dichloromethane fraction of 21 EC and 21 WP sprayed in suspension consentration of 1% on potted broccoli plant had residual activity around 16%-26,7% with range of half-lives of 5.9-7.7 days. Dichloromethane fraction at concentration of 0.2% and 0.3% formulated as EC did not cause phytotoxic to broccoli, tomato, chilli, soybean, red bean, onion, cucumber, and maize. Concerning with this potency, further studies are needed to identify insecticidal compounds.
PENGARUH CAMPURAN EKSTRAK AGLAIA HARMSIANA PERKINS DAN DYSOXYLUM ACUTANGULUM MIQ. (MELIACEAE) TERHADAP MORTALITAS DAN OVIPOSISI PLUTELLA XYLOSTELLA (L.) (LEPIDOPTERA: YPONOMEUTIDAE) Prijono, Djoko; Yuswanti, Lucy
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 4, No 1 (2004): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1645.745 KB) | DOI: 10.23960/j.hptt.141-7

Abstract

Effect of Mixture of Aglaia harmsiana Perkins and  Dysoxylum acuntangum Miq. (Meliaceae) Extracts on Mortality and Oviposition of Plutella xyostella (L.) (Lepidoptera: Yponomeutidae). This study was conducted to evaluate the effect of mixture of extracts of Aglaia harmsiana seeds and Dysoxylum acutangulum leaf petioles on mortality and oviposition of Plutella xyostella (L.) (Lepidoptera: Yponomeutidae). The insecticidal activity of ethyl acetate fraction of A. harmsiana and D. Acutangulum extracts and their mixture (concentration ratio of Ah and Da was 4:1) was evaluated against the thrid-instar P. xylostella was assessed by choice tests using broccoli seddlings. LC50 and LC95 of A. harmsiana extracts against the thirds the+fourth instar larvae were 0.054% and 0.129%, respectively, those of D. acutangulum extract were 0.031% and 0.103%, and those of the the mixture of A. harmsiana and D.acutangulum extract and the mixture at concentrations of 0.06% - 0.38%, 0.3%, and 0.02% - 0.3%, respectively, significantly reduced the number of eggs deposited by the female, P. xylostella on broccoli seedings, with oviposition inhibition indices of 0.30-0.59, 0.28-0.45 and 0.045-0.70. A.harmsiana ectract at all concentrations tested was phytotoxic to broccoli seedlings, whereas D. acutangulum extract was not. Mixture of A. harmsiana and D. acutangulum extract were phytotoxic to broccoli seedlings at concentrations of 0.06-0.3%. Thus, A. hamarsiana extract of mixture containing the extract should be used with caution and their phytotoxicity should be specifically tested on target crops before being used in the field.
Aktivitas insektisida ekstrak buah Piper aduncum L. (Piperaceae) dan Sapindus rarak DC. (Sapindaceae) serta campurannya terhadap larva Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) Yan Yanuar Syahroni; Djoko Prijono
Jurnal Entomologi Indonesia Vol 10 No 1 (2013): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.8 KB) | DOI: 10.5994/jei.10.1.39

Abstract

Crocidolomia pavonana is an important pest of Brassicaceous vegetable crops. Botanical insecticides are potential alternatives for the control of C. pavonana pest. Insecticidal activity of Piper aduncum and Sapindus rarak fruit extracts as well as their mixtures was evaluated against C. pavonana larvae in the laboratory. Ethyl acetate P. aduncum (EtPa) extract as well as methanolic S. rarak (MeSr) and aqueous S. rarak (AqSr) extracts were tested separately and in mixtures against second-instar larvae C. pavonana by a leaf-residue feeding method. At the LC95 level - 72 hours after treatment (HAT), EtPa extract was about 14.5 and 12.8 times, respectively, more toxic to C. pavonana larvae than MeSr and AqSr extracts. At the LC95 level, EtPa + MeSr extract mixture (1 : 10 w/w) was about 1.64 times more toxic to the test larvae than EtPa + AqSr extract mixture (1 : 10 w/w). Based on combination index at 48, 72, and 96 HAT, EtPa + MeSr extract mixture at LC50 level as well as EtPa + AqSr extract mixture at LC50 and LC95 levels had additive joint action, whereas EtPa + MeSr extract mixture at LC95 was weakly synergistic. In addition to lethal effect, the treatment with all test materials delayed the development of C. pavonana larvae from second-instar to fourth instar. Thus, EtPa, MeSr, and AqSr extracts as well as the mixtures between EtPa and S. rarak extracts are potential alternatives for the control of C. pavonana pest.
Physiological Response of Crocidolomia pavonana to the Calophyllum soulattri Active Fraction EDY SYAHPUTRA; DJOKO PRIJONO; DADANG DADANG; SYAFRIDA MANUWOTO; LATIFAH KOSIM DARUSMAN
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.314 KB) | DOI: 10.4308/hjb.13.1.7

Abstract

The objective of this study was to evaluate the physiological response of the cabbage head caterpillar Crocidolomia pavonana treated with an active fraction of Calophyllum soulattri bark extract. Extraction of the test plant materials were performed with maceration method using methanol, continued by counter-current distribution separation in ethylacetate and water. Methanol fractionation of C. soulattri was performed by vaccuum liquid chromatography and the bioassays were conducted by a leaf-feeding method. The results showed that the dichloromethane fraction of C. soulattri had strong insecticidal activity against C. pavonana larvae, with LC50 of 0.05%. Sublethal treatments with the active fraction at LC15, LC50, and LC85 reduced the relative growth rate of the fourth instars by 48.9-94.1%. The treatments with the fraction at LC15 and LC50 to the fourth instars reduced the activity of invertase and protease enzyme by 20.7-24.1 and 14.4-25.14%, respectively, but increased the activity of trehalase by 26.7-120% as compared with controls. Key words: Physiological response, Crocidolomia pavonana, Calophyllum soulattri, botanical insecticide