Garuda - Garba Rujukan Digital

Potency of Rhodotorula minuta as Plant Growth Promoter on Red Chili Plant Infected by Colletotrichum acutatum Sri Hartati; Linda Tarina; Endah Yulia; Luciana Djaya
CROPSAVER Vol 2, No 2 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Some yeast species were reported to have the ability to promote plant growth. Rhodotorula minuta is a yeast species that has the potency as biocontrol agent. This research was objected to evaluate the potency of R. minuta in promoting the growth of red chilli infected by Colletotrichum acutatum. The experiment was carried out at the Laboratory of Plant Protection Biotechnology and Green House of Ciparanje Experiment Station, Faculty of Agriculture, Universitas Padjadjaran. The experiment was arranged in the randomized completely block design with 9 treatments and 5 replications. The treatments were infestation of R. minuta and inoculation of C. acutatum on 5, 7, and 10 days after replanting, and a positive control with no R. minuta infestation but inoculated with the pathogen and a negative control without R. minuta infestation nor pathogen inoculation. Results of the experiment showed that the effectiveness of the treatments with R. minuta on the increase of the chilli plant height was 3.78-17.90%, leaf number was 4.09-15.19%, and dry weight of the biomass was 0.14-0.58%. This results indicated that treatments with R. minuta were able to promote the growth of chilli plants.

The Effect of Methyl Eugenol Block Plus on Bactrocera dorsalis Complex Total Captured in Chili Plantation Agus Susanto; Wahyu Daradjat Natawigena; Luciana Djaya; Tohidin Tohidin; Fauza Saputra
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.9708

Fruit flies (Bactrocera spp.) are an important pest for horticultural crops, especially fruits and vegetables. One of the most effective and eco-friendly methods to control male and female fruit flies is by usingtraps that use Methyl Eugenol (ME) block plus fruit essence as an attractant. The purposes of this research were to acquire the most effective formulation of ME Block plus fruit essence to catch the most male and female fruit flies on the chili plantation and to detremine the increase in total of fruit flies caught. This research started from December 2017 to January 2018 at Cibeureum Village, Sukamantri District, Ciamis Regency, West Java Province. The research was conducted using a randomized block design consisting of 8 treatments and 3 repetitions. The treatments consisted of adding fruit essence to the ME block: 2 ml of ME on cotton; 2 ml of ME block; 2 ml of ME block + 4 ml of mango essence; 2 ml of ME block + 4 ml of orange essence; 2 ml of ME block + 4 ml of guava essence; 2 ml of ME block + 4 ml of star fruit essence; 2 ml of ME block + 4 ml of chili essence; Antilat (Organic pesticide) as a comparison. The results showed that ME block plus fruit essence has increased the total of male fruit flies caught, but it did not attract female fruit flies. The best combinations with the highest average of male fruit flies caught were ME block plus orange essence with 750.67/5 week, followed by ME block plus chili essence with 746.00/5 week

Populasi Hama Wereng Batang Coklat (Nilaparvata Lugens Stal.) Dan Keragaman Serangga Predatornya Pada Padi Sawah Lahan Dataran Tinggi Di Desa Panyocokan, Kecamatan Ciwidey, Kabupaten Bandung Martua Suhunan Sianipar; Luciana Djaya; Entun Santosa; RC Hidayat Soesilohadi; Wahyu Daradjat Natawigena; Mochamad Ardiansyah
Agrikultura Vol 26, No 2 (2015): Agustus, 2015
Publisher : Fakultas Pertanian Universitas Padjadjaran

ABSTRACTPopulation of Brown Plant Hopper (Nilaparvata lugens Stal.) and the Diversity of Its Natural Enemyin Highland Paddy Rice Field in the Village of Panyocokan, Ciwidey District, Bandung RegencyBrown lanthopper/BPH (Nilaparvata lugens Stal.) is a main pest of rice in the field. This researchwas aimed to study the population of BPH and the diversity of its natural enemy in highland paddyrice field. The survey was conducted in three paddy plots (15 m x 20 m) located in the village ofPanyocokan, Ciwidey District, Bandung Regency, West Java. The experiment was conducted in thegreenhouse of the Department of Plant Pests and Diseases, Faculty of Agriculture, UniversitasPadjadjaran. The samples of insects were collected systematically using yellow trap and net trap,and visually observed in each rice cluster. The number of BPH were recorded and accumulatedeach week. Fecundity, life cycle, and sex ratio of the new generation of BPH were observed in 3replications by using 1 pair of WBC on each replication. The result showed that the population ofBPH in the highland was below 10, which means that BPH population was still below theeconomic threshold and control threshold. Temperature, humidity, and rainfall did notsignificantly affect the BPH population. This was indicated by the regression analysis of eachtemperature (Y = 0.557-8.167x; R2 = 0.039; P = 0.465), humidity (Y = -0.077+ 9.112x; R2 = 0.045; P =0.428), and rainfall (Y = -0.118 + 3.412x; R2 = 0.136; P = 0.159). Natural enemy diversity indextended to fluctuate widely from low to moderate. In the temperature range from 21.1°C to 34.8°C,BPH could produce 127-207 new generation during its lifetime. BPH needed an average of 37.66days to produce a new generation. The ratio of male to female was 1.06 : 1.Keywords: population, brown planthopper, diversity, predator, highland paddy rice field, Ciwidey,BandungABSTRAKHama wereng batang cklat/WBC (Nilaparvata lugens Stal.) merupakan hama utama tanaman padi.Penelitian ini bertujuan untuk mempelajari populasi WBC dan keragaman musuh alaminya padatanaman padi sawah di dataran tinggi. Penelitian dilakukan dengan metode survei dan eksperimen.Survei dilakukan pada 3 petak lahan percobaan berukuran 15 mx 20 m bertempat di DesaPanyocokan, Kecamatan Ciwidey, Kabupaten Bandung dan eksperimen dilakukan di rumah kacaDepartemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran. Metodepengambilan sampel dilakukan secara diagonal sistematis. Populasi WBC ditentukan denganmenggunakan perangkap kuning dan perangkap jaring dan dengan pengamatan langsung padarumpun padi. Eksperimen dilakukan dengan meletakkan sepasang WBC dalam wadah, lalu diamatikeperidian, siklus hidup, dan sex ratio keturunannya. WBC yang tertangkap dihitung jumlahnyadan diakumulasikan setiap minggunya. Hasil penelitian menunjukkan bahwa kepadatan populasi dilahan survey di bawah 10 ekor/rumpun yang artinya populasi WBC masih di bawah ambangekonomi atau ambang kendali. Suhu, kelembaban, dan curah hujan tidak memberikan pengaruhyang signifikan terhadap populasi WBC. Hal tersebut ditunjukkan dengan analisis regresi masingmasingpada suhu (Y= 0,557 – 8,167x; R2 = 0,039; P= 0,465), kelembaban (Y = -0,077 + 9,112x; R2 =0,045; P = 0,428), dan curah hujan (Y = -0,118 + 3,412x; R2 = 0,136; P = 0,159). Indeks keragamanmusuh alami cenderung mengalami fluktuasi dari rendah sampai sedang. Pada kisaran suhu 21,1oC–34,8oC, hasil pengamatan keperidian menunjukkan WBC dapat menghasilkan 127-207 individubaru selama masa hidupnya. Pada pengamatan siklus hidup, WBC memerlukan rata-rata 37,66 harisampai menghasilkan generasi baru. Pengamatan sex ratio menunjukkan perbandingan (jantan :betina) 1,06 :1.Kata kunci: populasi, wereng batang coklat, keragaman, predator, sawah, dataran tinggi, Ciwidey,Bandung

Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi Noor Istifadah; Muhamad Salman Umar; Sudarjat Sudarjat; Luciana Djaya
Agrikultura Vol 27, No 3 (2016): Desember, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

Kemampuan Bacillus subtilis dan Trichoderma harzianum dalam Campuran Serat Karbon dan Silika Nano untuk Meningkatkan Ketahanan Tanaman Padi Terhadap Penyakit Blas (Pyricularia oryzae) Hersanti Wartono; Nurul Safitri; Luciana Djaya; Martua Suhunan Sianipar
Agrikultura Vol 31, No 3 (2020): Desember, 2020
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v31i3.29483

Penyakit blas yang disebabkan Pyricularia oryzae merupakan salah satu penyakit penting pada tanaman padi di Indonesia. Pengendalian yang dapat digunakan untuk mengendalikan penyakit blas ialah menggunakan agens biokontrol. Dalam penelitian ini bakteri Bacillus subtilis dan jamur Trichoderma harzianum diformulasikan dengan bahan pembawa berupa serat karbon dan diperkaya dengan unsur hara mikro berupa silika dalam ukuran nano. Penelitian ini dilakukan untuk menguji kemampuan B. subtilis dan T. harzianum yang diaplikasikan secara tunggal maupun kombinasi, dengan dan tanpa campuran serat karbon dan silika nano, dalam meningkatkan ketahanan tanaman padi terhadap penyakit blas. Pengujian pada tanaman padi dilakukan dengan merendam benih dan akar semai padi dalam delapan perlakuan formulasi. Percobaan disusun menggunakan rancangan acak kelompok. Hasil percobaan menunjukkan bahwa aplikasi B. subtilis dan T. harzianum, baik secara tunggal maupun kombinasi, mampu meningkatkan ketahanan tanaman padi terhadap penyakit blas dengan penghambatan penyakit berkisar antara 15,64% - 21,59%. Selain itu, aplikasi tunggal maupun kombinasi B. subtilis dan T. harzianum yang diformulasikan dengan serat karbon dan silika nano mampu meningkatkan ketahanan tanaman padi terhadap penyakit blas, dengan penghambatan penyakit berkisar antara 18,75% - 25,12%.

Bacillus subtilis dan Lysinibacillus sp. (CK U3) dalam Serat Karbon dan Silika Nano Menekan Pertumbuhan Fusarium oxysporum f.sp. lycopersici dan Perkembangan Penyakit Hawar Kecambah Tomat Hersanti Wartono; Nurul Hidayati Emilia; Luciana Djaya; Endah Yulia
Agrikultura Vol 32, No 2 (2021): Agustus, 2021
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v32i2.33387

Fusarium oxysporum f.sp. lycopersici (Fol) merupakan patogen yang dapat menginfeksi pada semua fase pertumbuhan tanaman tomat, mulai dari semai sampai fase generatif. Salah satu metode yang dapat digunakan untuk mengendalikan patogen ini yaitu dengan memanfaatkan agen pengendali hayati, diantaranya Bacillus subtilis dari kelompok Plant Growth Promoting Rhizobacteria (PGPR) dan bakteri endofit Lysinibacillus sp. Kedua bakteri diformulasikan dalam serat karbon sebagai bahan pembawa dan diperkaya dengan unsur hara silika yang berukuran nano. Penelitian ini bertujuan untuk menguji kemampuan B. subtilis dan Lysinibacillus sp. (CK U3) dalam serat karbon dan silika nano untuk menghambat pertumbuhan koloni Fol dan menekan perkembangan penyakit yang disebabkan oleh Fol pada benih tomat. Suspensi B. subtilis dan Lysinibacillus sp. (CK U3) diformulasikan secara tunggal dan campuran dalam serat karbon 80 mesh dan silika nano 1%. Percobaan menggunakan Rancangan Acak Lengkap dan dilakukan dalam dua tahap. Pertama, pengujian terhadap penghambatan pertumbuhan koloni Fol yang terdiri atas delapan perlakuan dan tiga ulangan. Kedua, pengujian terhadap penekanan perkembangan penyakit yang disebabkan oleh Fol pada benih tomat yang terdiri atas delapan perlakuan dan tiga ulangan. Hasil penelitian menunjukkan B. subtilis dalam serat karbon dan silika nano mampu menghambat pertumbuhan koloni Fol sebesar 59,49% dan menekan perkembangan penyakit hawar kecambah benih tomat sebesar 66,7%.

Pengaruh Induksi Resistensi oleh Khamir Candida tropicalis terhadap Pertumbuhan Tanaman Cabai Terinfeksi Colletotrichum acutatum Sri Hartati; Linda Tarina; Endah Yulia; Luciana Djaya
Agrikultura Vol 30, No 1 (2019): April, 2019
Publisher : Fakultas Pertanian Universitas Padjadjaran

ABSTRACTEffect of induced resistance by Candida tropicalis on the growth of chili plants infected by Colletotrichum acutatumColletotrichum acutatum is a major pathogen of chili that causes a big yield loss. Candida tropicalis has been reported as a biocontrol agent antagonistic to C. acutatum. The research was objected tofind out the effect of induced resistance by C. tropicalis on the growth of chili plants infected by C. acutatum. The application of the yeast was carried out by seed dipping and seedling spraying methods. As treatments, C. acutatum, the pathogen was inoculated at different time, i.e. A = inoculation at 3 days after the induction (dai) by C. tropicalis, B = inoculation at 7 dai, C = inoculation at 10 dai, D = positive control on inoculation at 3 dai, E = positive control on inoculation at 7 dai, F = positive control on inoculation at 10 dai, G = negative control with no induction nor inoculation. The results showed that the induction by C. tropicalis was able to increase the plant height, leaf number, and dry weight. The highest increase of plant height of 30.72% was caused by induction of C. tropicalis inoculated at 7 dai. Induction of C. tropicalis inoculated at 3 dai increased the leaf number by 49.47%, and the plant dry weight by 50%. C. tropicalis has a potency to be developed as plant growth inducer.Keywords: Antagonist, Inoculation, Fungi, YeastABSTRAKJamur Colletotrichum acutatum merupakan patogen penyebab kehilangan hasil panen yang cukup besar pada tanaman cabai. Salah satu mikrob antagonis jamur ini yang telah dilaporkan adalah khamir Candida tropicalis. Penelitian ini bertujuan untuk mengetahui pengaruh induksi resistensioleh C. tropicalis terhadap pertumbuhan tanaman cabai terinfeksi C. acutatum. Penelitian dilaksanakan dengan menggunakan teknik perendaman benih dan penyiraman bibit tanaman cabaimenggunakan suspensi C. tropicalis dengan waktu inokulasi patogen C. acutatum yang berbeda. Perlakuan tersebut adalah A = Inokulasi C. acutatum 3 hari setelah perlakuan induksi C. tropicalis (hsp), B = Inokulasi C. acutatum 7 hsp, C = Inokulasi C. acutatum 10 hsp, D = Kontrol (+) dengan inokulasi C. acutatum 3 hsp, E = Kontrol (+) dengan inokulasi C. acutatum 7 hsp, F = Kontrol (+) dengan inokulasi C. acutatum 10 hsp, G = Kontrol (-) tanpa induksi dan inokulasi. Hasil penelitianmenunjukkan bahwa perlakuan induksi resistensi oleh khamir C. tropicalis mampu meningkatkan tinggi, jumlah daun, dan bobot kering tanaman cabai. Kemampuan meningkatkan tinggi tanamancabai tertinggi terjadi pada perlakuan C. tropicalis dengan waktu inokulasi 7 hsp yaitu sebesar 30,72%. Perlakuan khamir C. tropicalis dengan inokulasi patogen 3 hsp mampu meningkatkan jumlah daun tanaman cabai sebesar 49,47%. Perlakuan C. tropicalis dengan waktu inokulasi 3 hsp mampu meningkatkan bobot kering tanaman sebesar 50%. Khamir C. tropicalis berpotensi untuk dikembangkan sebagai agens pemacu pertumbuhan tanaman.Kata Kunci: Antagonis, Inokulasi, Jamur, Patogen

Potency of Yeasts to Promote Induced Resistance on Chili Plant (Capsicum annuum L.) against Yellow Leaf Curl Disease Sri Hartati; Rika Meliansyah; Luciana Djaya; Ceppy Nasahi; Ramadhan Kamal Putra
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.52089

Yellow leaf curl is a major disease on chili plants. The use of antagonistic yeasts as the control agents is a good alternative in an environmentally friendly control method. This study was objected to evaluate the potencies of Rhodotorula minuta and Candida tropicalis to promote induced resistance on chili plants against yellow leaf curl disease. The experiment was arranged in the randomized complete block design with 9 treatments and 3 replications. The treatments were the application of the yeasts at three different times of virus inoculation, as follows: A. R. minuta, virus inoculation at 3 days after transplanting (dat), B. R. minuta, virus inoculation at 7 dat, C. R. minuta, virus inoculation at 10 dat, D. C. tropicalis, virus inoculation at 3 dat, E. C. tropicalis, virus inoculation at 7 dat, F. C. tropicalis, virus inoculation at 10 dat, G. control, virus inoculation at 3 dat, H. control, virus inoculation at 7 dat, and I. control, virus inoculation at 10 dat. No treatment was applied to the control. The yeasts were applied by soaking the chili seeds, and pouring the suspension into the growth media at transplanting. The variables observed were incubation period, disease severity, and disease incidence. Peroxidase activity, phenylalanine ammonia lyase activity, and salicylic acid accumulation were also analyzed. The results showed that the application of R. minuta or C. tropicalis was able to extend the disease incubation period, but did not reduce the yellow leaf curl disease incidence and severity. The treatment of R. minuta, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6870.93 units (0.5 minute) and from 577.367 units to 1131.300 units (2.5 minutes), PAL activity from 16.059 to 17.911 A290/mg, and accumulation of salicylic acid from 2.785 to 6.263 ppm. Application of C. tropicalis, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6033.067 units (0.5 minute) and from 577.367 units to 950.967 units (2.5 minutes), and accumulation of salicylic acid from 2.785 to 6.982 ppm.

Title

Found 8 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 8 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search