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All Journal As-syifaa Jurnal Farmasi Window of Health : Jurnal Kesehatan
Seniwati Dali
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Total Flavonoid Levels of Ethanol Extract of Lemongrass Leaves and Thatch Grass Leaves Using UV-VIS Spectrophotometry Nuryadin, Yudhi; Naid, Tadjuddin; Dahlia, Andi Amaliah; Dali, Seniwati
Window of Health : Jurnal Kesehatan Vol. 1 No. 4 (Oktober, 2018)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.445 KB)

Abstract

Lemongrass Leaves (Cymbopogoncitratus DC.) and Thatch Grass Leaves (Imperata cylindrical (L.)Raeusch) are medicinal plants belong to similary family. Their activity as natural antioxidant sources because it’s contains the flavonoids compound. This study aimed to determine the total flavonoid content on the ethanol extract of lemongrass leaves and the thatch grass leaves using UV-Vis spectrophotometry. The extraction wasdone by maceration methodusing ethanol. The first sample is 3,95% lemongrass leaves, and the second one is 2,98% thatch grass leaves. The qualitative analysis by TLC method with the eluent ratio n-Hexane:ethyl acetate (6:4) showed that both samples positively contain flavonoids. The quantitative analysis by spectrophotometry at the maximum wavelength 431 nm showed that total flavonoid content in the first sample is 2,8666 % and the second sample is 3,4866 %.
Total Flavonoid Levels of Ethanol Extract of Lemongrass Leaves and Thatch Grass Leaves Using UV-VIS Spectrophotometry Nuryadin, Yudhi; Naid, Tadjuddin; Dahlia, Andi Amaliah; Dali, Seniwati
Window of Health : Jurnal Kesehatan Vol. 1 No. 4 (Oktober, 2018)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.445 KB) | DOI: 10.33368/woh.v1i4.107

Abstract

Lemongrass Leaves (Cymbopogoncitratus DC.) and Thatch Grass Leaves (Imperata cylindrical (L.)Raeusch) are medicinal plants belong to similary family. Their activity as natural antioxidant sources because it’s contains the flavonoids compound. This study aimed to determine the total flavonoid content on the ethanol extract of lemongrass leaves and the thatch grass leaves using UV-Vis spectrophotometry. The extraction wasdone by maceration methodusing ethanol. The first sample is 3,95% lemongrass leaves, and the second one is 2,98% thatch grass leaves. The qualitative analysis by TLC method with the eluent ratio n-Hexane:ethyl acetate (6:4) showed that both samples positively contain flavonoids. The quantitative analysis by spectrophotometry at the maximum wavelength 431 nm showed that total flavonoid content in the first sample is 2,8666 % and the second sample is 3,4866 %.
Aktivitas Antioksidan Ekstrak Etanol Buah Merah (Pandanus conoideus Lam.) dengan Metode FRAP Wabula, Rheytno Asdin; Dali, Seniwati; Widiastuti, Harti
Window of Health : Jurnal Kesehatan Vol. 2 No. 4 (Oktober, 2019)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (901.39 KB) | DOI: 10.33368/woh.v0i0.203

Abstract

Red fruit (Pandanus conoideus Lam.) Is an endemic plant that grows in the Papua region. Based on previous research, red fruit contains several active compounds with a fairly high level, including carotenoids, tocopherol, beta-carotene, α tocopherol, and fatty acids such as oleic acid, linoleic acid, linolenic acid, and dexoic acid. The high tocopherol content in red fruit has antioxidant activity. This study aims to determine the antioxidant capacity of the ethanol extract of red fruit (Pandanus conoideus Lam.) Using the FRAP method. Three replications of red fruit ethanol extract were measured for antioxidant capacity using standard α-tocopherol solution in a UV-Vis spectrophotometer at a maximum wavelength of 757 nm. The results showed that the sample of red fruit ethanol extract (Pandanus conoideus Lam.) Had total antioxidant activity with an activity of 1,392 x 10-3 g ATE / g extract.
Isolasi Bakteri Rhizosfer Tanaman Nilam (Pogostemon cablin Benth.) yang Berpotensi sebagai Penghasil Senyawa Antibakteri terhadap Bakteri Penyebab Infeksi Saluran Pencernaan Maulidiyah, Zakiya; Dali, Seniwati; Rusli, Rusli; Naid, Tadjuddin
Window of Health : Jurnal Kesehatan Vol. 3 No. 2 (April, 2020)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.974 KB) | DOI: 10.33368/woh.v0i0.295

Abstract

Research on isolation of rhizosphere bacteria in patchouli (Pogostemon cablin Benth.) Plants. This study aims to obtain patchouli plant rhizosphere bacteria which have the potential to produce antibacterial compounds against bacteria that cause digestive tract infections. The first stage, isolation of bacteria originating from the patchouli plant rhizosphere. Antibacterial screening test using a match method, obtained IBRN-3 and IBRN-5 isolates inhibited the growth of all test bacteria. IBRN-3 and IBRN-5 isolates were fermented. The fermentation results are extracted with ethyl acetate solvent to produce dry fermentate ethyl acetate extract. Thin Layer Chromatography was identified on the ethyl acetate extract of IBRN-3 and IBRN-5 using chloroform: methanol (8: 1) eluent and followed by TLC-Bioautography test. The results of spotting on IBRN-3 with Rf value 0.93 inhibited the bacteria E. coli, S. aureus, Rf value 0.82 inhibited B. subtillis, S. dysenteriae, Rf value 0.64 inhibited S. aureus and S. thypii, Rf value 0.89 , 0.36, 0.24 inhibited S. dysenteriae, Rf values ​​0.84 and 0.74 inhibited S. aureus. Whereas for IBRN-5, spots were obtained with an Rf value of 0.91 inhibiting B. subtillis, E. coli S. thypii, an Rf value of 0.95 inhibiting S. aureus S. dysenteriae, an Rf value of 0.84 inhibiting B. subtillis, S. thypii, Rf value of 0.83 inhibits S. dysenteriae, and Rf value of 0.74 inhibits S. thypii.
AKTIVITAS ANTIOKSIDAN FRAKSI RIMPANG KENCUR (KAEMPFERIA RHIZOMA) DENGAN MENGGUNAKAN METODE PEREDAMAN 1,1 DIPHENYL-2-PICRYLHYDRAZIL (DPPH) Muhafidzah, Zahrah; Dali, Seniwati; Syarif, Rezky Amriati
Jurnal Ilmiah As-Syifaa Vol 10, No 1 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.818 KB)

Abstract

Kaempferia is one of the most widely used plants in society is a traditional at the medicine. Empirically Kaempferia rhizome has a special quality as a wound healer, good for digestion, antibacterial, and as an antioksidant. Based on research been conducted that Kaempferia extract has antioxidant activity. This research aimed to test antioxidant activity base on IC50 value on kaempferia rhizome fraction by the reduction method 1,1 diphenil-2-phicrylhydrazil (DPPH). The extract obtained was fractionated using a vacuum liquid chromatography method, then performed free radical reduction meansurements using UV-VIS spectrophotometry. A positive fraction of antioxidants activity is the n-hexane fraction : ethyl acetate (7:3 and 8:2). The n-hexane fraction 8:2 showed higher antioxidant activity with IC50  731.832 µg/mL. The n-hexane fraction: ethyl acetate 7:3 with IC50  829.737 µg/mL
ISOLASI KITOSAN DARI LIMBAH CANGKANG KEPITING BAKAU (Scylla serrata) DAN APLIKASINYA TERHADAP PENYERAPAN TRIGLISERIDA Dali, Seniwati; Safitri, Nur Ramadhana Dewi; Fawwaz, Muammar
Jurnal Ilmiah As-Syifaa Vol 8, No 2 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.392 KB) | DOI: 10.33096/jifa.v8i2.200

Abstract

Chitosan is the result of deacetylation process from chitin which it can be found on Crustacean outer shell such as crabs. Chitosan can bind fat if it was consumed by human. The fat-binding ability of chitosan depends on the deacetylation degree. The research have been made into two phases. The first phase, chitosan was made from crab shell using NaOH. The deacetylation degree from chitosan that was made from the earlier process was analyzed with FTIR. The deacetylation degree result of the research was 59.39% using NaOH 50%. The second phase was the process of adsorbing triglycerides using chitosan in 10, 30, 45, and 60 minutes which was analyzed using spectrophotometer UV-Vis. The result of this research showed that the optimum triglycerides with 0.5, 1, 3 gram of chitosan was 2.99%, 3.14%, and 3.36%.
Co-Authors A Amaliah Dahlia Harti Widiastuti Maulidiyah, Zakiya Muammar Fawwaz Muhafidzah, Zahrah Nasution, Annio Indah Lestari Nur Ramadhana Dewi Safitri, Nur Ramadhana Dewi Nuryadin, Yudhi Nuryadin, Yudhi R. Rusli Syarif, Rezky Amriati Tadjuddin Naid Wabula, Rheytno Asdin