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All Journal Biosfer: Jurnal Tadris Biologi Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Eti Ernawiati
Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung
Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences Other

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Cytological analysis of Kepok Abu Polyploid's Banana Plantlets (Musa paradisiaca L.) Due to Colchicine's Application in Tissue Culture Media Istiqomah istiqomah; Sri Wahyuningsih; Eti Ernawiati; Rochmah Agustrina
Biosfer: Jurnal Tadris Biologi Vol 12, No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.032 KB) | DOI: 10.24042/biosfer.v12i2.8501

Abstract

This research aimed to determine the mitotic abnormalities, changes in the number of chromosomes, mitotic index, and the number and length of kepok abu banana plantlet roots due to administration of 0.1% colchicine in tissue culture media. This research was performed at the Botanical Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, UNILA. This research compared the cytology by employing the Squash method between root tip cells of kepok abu banana plantlets after administering 0.1% colchicine and the control group. The researchers analyzed the data descriptively and presented them in tables and bar charts. The results showed that the administration of 0.1% colchicine resulted in mitotic abnormalities. It also increased the number of chromosomes in the controls 2n=3x and 2n=3x+4. The mitotic index decreased, and the average number of roots was lower. However, the size was longer compared to the control group.
Aplikasi Penggunaan Alat Pengering Pengganti Oven untuk Pembuatan Herbarium di Kebun Raya Liwa Kabupaten Lampung Barat Yulianty; Amir supriyanto; Eti Ernawiati; Lili Chrisnawati
Bahasa Indonesia Vol 19 No 03 (2022): Sarwahita : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.193.5

Abstract

One of the countries with high biodiversity is Indonesia. The potential of existing plants has not been maximally empowered by the community, especially in the Liwa Botanical Gardens. The involvement of the staff in the KRL is very necessary to be able to directly collect the observed plants and make a herbarium. Constraint faced in making herbarium is in the drying process. So far, the herbarium drying process is still being carried out in Department of Biology, University of Lampung. This PKM activity will begin with a pre-test to determine the initial ability of KRL staff on the importance of the drying process and the practice of making simple herbarium dryers instead of ovens. At the end of the activity, KRL staff were given a post-test to determine the increase in knowledge about the importance of drying herbariums. With this PKM activity, KRL staff increase their knowledge and skills in using simple tools for drying plant samples and in the long term the availability of more diverse herbariums in an effort to conserve potential plants in KRL. The result of this activity was an increase in score points by 28.08 and a percentage increase in knowledge of 51.63%.
Co-Authors Agustrina, Rochmah Amir supriyanto Istiqomah istiqomah Lili Chrisnawati Sri Wahyuningsih Yulianty