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All Journal Bioscience Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Techno: Jurnal Penelitian BIOMA : Jurnal Biologi Makassar Jurnal Penelitian Pertanian Terapan Jurnal SOLMA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Biosilampari: Jurnal Biologi Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Medika Malahayati Floribunda Jurnal Ilmiah Farmasi Farmasyifa Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun International Journal of Engineering, Science and Information Technology Journal of Sustainability Science and Technology JURNAL BIOLOGI PAPUA AMMA : Jurnal Pengabdian Masyarakat
Sri Wahyuningsih
Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Transportation Other

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Cytological analysis of Kepok Abu Polyploid's Banana Plantlets (Musa paradisiaca L.) Due to Colchicine's Application in Tissue Culture Media Istiqomah istiqomah; Sri Wahyuningsih; Eti Ernawiati; Rochmah Agustrina
Biosfer: Jurnal Tadris Biologi Vol 12, No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.032 KB) | DOI: 10.24042/biosfer.v12i2.8501

Abstract

This research aimed to determine the mitotic abnormalities, changes in the number of chromosomes, mitotic index, and the number and length of kepok abu banana plantlet roots due to administration of 0.1% colchicine in tissue culture media. This research was performed at the Botanical Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, UNILA. This research compared the cytology by employing the Squash method between root tip cells of kepok abu banana plantlets after administering 0.1% colchicine and the control group. The researchers analyzed the data descriptively and presented them in tables and bar charts. The results showed that the administration of 0.1% colchicine resulted in mitotic abnormalities. It also increased the number of chromosomes in the controls 2n=3x and 2n=3x+4. The mitotic index decreased, and the average number of roots was lower. However, the size was longer compared to the control group.
Identification of Disease and Efforts to Protect Natural Orchid Plants Against Fungi Infection in the Liwa Botanical Garden Mahfut Mahfut; Mitha Valentina Treesya Panjaitan; Sri Wahyuningsih; Tundjung Tripeni Handayani; Sukimin Sukimin
International Journal of Engineering, Science and Information Technology Vol 1, No 1 (2021)
Publisher : Master Program of Information Technology, Universitas Malikussaleh, Aceh Utara, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.665 KB) | DOI: 10.52088/ijesty.v1i1.39

Abstract

Liwa Botanical Garden is located in West Lampung Regency which has an area of 86.6 hectares and is one of the conservation of ornamental plants that presents the flora of the Bukit Barisan Selatan National Park. One of the flagship collections of this botanical garden is a native orchid. Disease infection is still a major obstacle in the cultivation and development of native orchid potential at Liwa Botanical Garden. Based on the results of previous studies, it is known that several individual natural orchid collections from Liwa Botanical Garden showed symptoms of fungal disease infections. Disease infections can inhibit plant growth and resistance and reduce the aesthetic value periodically. Symptoms of infection include necrotic patches on the upper surface of the leaf. This research was conducted to determine the identification of diseases and efforts to protect native orchids against fungal infections at Liwa Botanical Garden through a collection of samples that showed symptoms of infection, analysis of disease symptoms, and analysis of disease resistance levels. The results showed that the response of the native orchid at Liwa Botanical Garden to fungal infections was showed symptoms of wilting in the leaves, namely in the orchids Eria sp., Dendrobium sp., and Cymbidium sp. turns black on the edge of the leaf, Thrixspermum sp. The type of orchid most commonly infected with fungus is Cymbidium sp. a total of 8 samples, and Thrixspermum sp. 3 samples, Eria sp. as many as 4 samples, Dendrobium sp. and Calanthe triplicata as many as 6 samples. The type of native orchid that is most susceptible to fungal infections is Dendrobium sp. and Eria sp. with disease intensity of 74.11% and 22.3%. The results of this activity are expected to be basic information in efforts to protect plants against diseases to support the application of conservation of native orchids on Liwa Botanical Garden.
THE EFFECT OF POLYETHYLENE GLYCOL (PEG) 6000 ON GREEN MUSTARD (Brassica juncea L.) GERMINATION AND GROWTH Garri Abdi Nusantara; Tundjung T. Handayani; Zulkifli Zulkifli; Sri Wahyuningsih
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 2 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i2.64

Abstract

The frequent drought in Indonesia has caused many farmers to experience a decline in vegetable production, one of which is green mustard. The green mustard is not resistant to drought, so water supply is an aspect that must be given special attention in cultivating green mustard. The objective of this study was to know whether the seedling and growth of seedling of green mustard (Brassica juncea L.) can grow well under drought stress by Polyethylene glycol 6000. This study was conducted in the Laboratory of Plant Physiology, Department of Biology, Faculty of Mathematics and Natural Science, the University of Lampung in 2019. This study was conducted in a Completely Randomized Design with the main factor is Polyethylene glycol 6000 with three levels: 0% w/v, 10% w/v, 20% w/v, and 30% w/v. Each combination of treatments was repeated five times. The number of the experimental unit was 20. The observed variables in this study were the percentage of seed germination, length of leaf, the width of leaf, a,b chlorophyll content, and total chlorophyll content. The data was homogenize using the Levene test, then continued to analyze using ANOVA at a 5% significant level and proceed with HSD test significant level 5% to determine the differences between the treatment. The result showed that under drought stress by Polyethylene glycol 6000 started to affect green mustard seedling and growth of seedling at the level of PEG 6000 concentration 20% w/v while the number of leaves not affected. The conclusion was PEG 6000 gave a negative impact on the green mustard and the green mustard induced by PEG 6000 10% w/v more adaptive than PEG 6000 20 w/v.
Efek Induksi Rhizoctonia pada Ketebalan Daun Anggrek yang diinfeksi Odontoglossum ringspot virus (ORSV) Sahira Josy Arifannisa; Sri Wahyuningsih; Tundjung Tripeni Handayani; Mahfut Mahfut
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 3 (2021): October 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i3.4372

Abstract

Anggrek memiliki nilai ekonomis yang tinggi sehingga sangat potensial untuk dibudidayakan. Jenis yang paling banyak diminati masyarakat adalah Dendrobium dan Phalaenopsis. Namun terdapat kendala utama yang dapat memengaruhi pertumbuhan anggrek yaitu infeksi virus. Jenis virus yang dilaporkan paling banyak menginfeksi adalah Odontoglossum ringspot virus (ORSV). Infeksi virus ini dapat menghambat pertumbuhan dan ketahanan tanaman, menurunkan kualitas bunga dan nilai estetika serta daya jual. Didapati pula gejala infeksi virus berupa mosaik, klorotik, streak, dan nekrosis. Salah satu upaya untuk mengatasi infeksi virus adalah dengan memanfaatkan mikoriza. Mikoriza yang dapat digunakan yaitu Rhizoctonia sp. Asosiasi mikoriza pada tumbuhan anggrek dapat memberikan pengaruh positif pada penyerapan nutrisi dan pertumbuhan inangnya, sehingga diharapkan dapat melindungi anggrek dari infeksi virus. Tujuan penelitian ini untuk mengetahui efek induksi Rhizoctonia pada karakter anatomi daun Phalaenopsis amabilis dan Dendrobium discolor yang diinfeksi ORSV dan mengetahui apakah terdapat perbedaan anatomi diantara kedua anggrek tersebut. Penelitian dilakukan di Laboratorium Botani Biologi FMIPA Unila dengan menggunakan Rancangan Acak lengkap Faktorial. Hasil dari penelitian ini adalah terdapat perbedaan ketebalan daun antara daun yang diinokulasi virus dan daun dengan perlakuan mikoriza. Daun anggrek dengan perlakuan mikoriza memiliki ketebalan tertinggi sedangkan yang terinfeksi virus memiliki ketebalan terendah.
Variasi Respon Anggrek Hasil Induksi Rhizoctonia Terhadap Infeksi Odontoglossum ringspot virus (ORSV) Mahfut Mahfut; Fania Nur Izzati; Eti Ernawiati; Sri Wahyuningsih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 7, No 1 (2022): February 2022
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v7i1.4373

Abstract

Anggrek sangat terkenal karena memiliki corak, bentuk, ukuran, dan warna bunga beranekaragam. Phalaenopsis dan Dendrobium termasuk jenis anggrek yang diminati sehingga produksinya perlu ditingkatkan secara kuantitas maupun kualitas. Adanya infeksi virus Odontoglossum ringspot pada anggrek menjadi salah satu kendala dalam budidaya anggrek. Odontoglossum ringspot virus (ORSV) merupakan virus yang banyak menginfeksi anggrek dan menimbulkan gejala pada bagian daun berupa mosaik, nekrosis, klorotik, kelayuan serta daun menggulung. Upaya pengendalian infeksi ORSV dapat memanfaatkan mikroorganisme, seperti Rhizoctonia yang akan berasosiasi dengan perakaran anggrek membentuk simbiosis mutualisme yang kemudian dapat menyediakan nutrisi bagi anggrek. Tujuan penelitian ini adalah untuk mempelajari respon anggrek Phalaenopsis amabilis dan Dendrobium discolor terinduksi Rhizoctonia terhadap infeksi ORSV. Penelitian dilaksanakan dengan Rancangan Acak Lengkap (RAL) faktorial menggunakan 6 perlakuan dan 4 ulangan. Hasil penelitian menunjukkan bahwa Phalaenopsis amabilis lebih rentan terinfeksi ORSV daripada Dendrobium discolor, dengan gejala yaitu daun mosaik, nekrotik, dan malformasi pada kisaran waktu 11 hari setelah inokulasi dengan Rhizoctonia . Hal ini mengindikasikan bahwa inokulasi Rhizoctonia pada Dendrobium discolor yang terinfeksi ORSV memberikan ketahanan yang lebih baik dibandingkan pada Phalaenopsis amabilis.  
ANALISIS BROWNING BUAH PISANG KEPOK (Musa paradisiaca L.) SETELAH PERLAKUAN ASAM ASKORBAT DAN LIDAH BUAYA (Aloe barbadensis L.) Ratri Mauluti Larasati; Martha Lulus Lande; Zulkifli Zulkifli; Sri Wahyuningsih
Jurnal Penelitian Pertanian Terapan Vol 19 No 1 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i1.1400

Abstract

Fruit is a necessity for most Indonesian people. Kepok banana is a climacteric fruit that can experience browning quickly. Therefore, this study was conducted to find a safe and effective material to prevent the browning process of kepok bananas. This study aims to determine the differences in the browning index, and the activity of the enzyme dehydrogenase in kepok banana cells. This study was conducted using a 2x3 factorial design. Factor A is ascorbic acid with 2 concentration levels namely 0% (b / v) and 5% (b / v). Factor B is Aloe (Aloebarbadensis L.) leaf extract with 3 levels, namely 0%, 5%, and 10%. The quantitative parameters are browning index and total dissolved carbohydrate content. The qualitative parameter is dehydrogenase enzyme activity. Levene test and variance analysis were carried out with 5% real level. The results obtained were kepok bananas with treatments coloured brighter than control. Ascorbic acid and Aloe (Aloe barbadensis L.) affect the browning index and dissolved total carbohydrate content of the sample. A decrease in dehydrogenase enzyme activity happened in the ascorbic acid treatment. Conclusions obtained from the study are ascorbic acid with a concentration of 5% can reduce the browning index of kepok banana by 31%, ascorbic acid with a concentration of 5% can maintain total dissolved carbohydrate content kepok banana as much as 53%, and Aloe extract 10% retain total dissolved carbohydrate content sample is 20%.
Pengaruh Pemberian Air Kelapa (Cocos nucifera L.) Dan Atonik Terhadap Pertumbuhan Tanaman Cabai Merah Besar (Capsicum annuum L.) Hasti Maulidya Fassya; Tundjung Tripeni Handayani; Sri Wahyuningsih; Mahfut Mahfut
TECHNO: JURNAL PENELITIAN Vol 9, No 1 (2020): Techno Jurnal Penelitian
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tjp.v9i1.1681

Abstract

Large red chili pepper (Capsicum annuum L.) has a fairly complete nutrition and economic value. The supply of large red chili in the country is considered low compared to its demand, that’s why we need to increase its production. Increasing production can be done by fertilizing using natural fertilizers is coconut water (Cocos nucifera L.) and administration of Growth Regulatory Substances (ZPT) is atonik. The purpose of this research is to determine the effect of coconut water and atonic to the growth of large red chilli pepper sprouts determine which consentration of coconut water and atonic that has the most effective effect to the growth of large chilli pepper. This research was conducted in October - November 2019 at the Botany Laboratory, Department of Biology, Faculty of Mathematics and Science, University of Lampung. This research was carried out using a 2x3 factorial design using young coconut water (a) with 3 levels of concentration ie 0% v / v (a1), 25% v / v (a2) and 50% v / v (a3) and atonic solution ( b) with 2 concentrations, namely 0% v / v (b1) and 10% v / v (b2). Data analysis is the data homogeneous first with the Levene test at 5% significance level. Data analysis using analysis of variance (Analysis Of Variance) ɑ = 5%. If the interaction between the two factors is real, then proceed with the determination of the simple effect of coconut water at each level of atonic concentration with the F test at 5% significance level. The results of this research shows that the use of atonic and coconut water effects the growth of large red chili plants, and the most effective concentration to the growth of large red chilli pepper plants is the combination of 0% coconut water + 10% atonic.
Ceratorhiza Induction towards ORSV Infection on Viability of Phalaenopsis and Dendrobium Valentina Dwi Anggita Sari; Mahfut Mahfut; Sri Wahyuningsih; Tundjung Tripeni Handayani
TECHNO: JURNAL PENELITIAN Vol 10, No 1 (2021): Techno Jurnal Penelitian
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tjp.v10i1.3041

Abstract

Orchids have a high level of biodiversity, such as Dendrobium and Phalaenopsis, which are epiphytes. Dendrobium can adapt to the condition of where it lives while Phalaenopsis can grow in highlands and depends on sunlight and humidity. Virus infection has become one of the obstacles in cultivating Dendrobium and Phalaenopsis. Efforts to increase fitness and control in Dendrobium and Phalaenopsis cultivation can be done by inducing the plant’s fitness using a mycorrhiza, such as Ceratorhiza. A mycorrhiza is a form of mutualism between fungi and the plant’s root. This research is aimed to give information related to the utilization of Cerathoriza for inducing orchids to suppress Odontoglossum ringspot virus (ORSV) infection, giving it better growth. The research was done in February-March 2021 at Botany Laboratory University of Lampung. A completely randomized factorial design was used on two factors, kind of orchid and mycorrhiza treatment (M), virus (V), and mycorrhiza virus (MV). Variables examined in this research are the amount of living and dead roots and leaves. Data obtained is homogenized using Levene’s test and continued by ANOVA with the significance level of 5% and further testing using Tukey’s test with the significance level of 5%. From this research, it is known that interaction between Phalaenopsis and Dendrobium exists during virus and mycorrhiza administration. It is concluded that Phalaenopsis anabilis is more vulnerable than Dendrobium discolor.
Identification of dendrobium Natural Orchids in Liwa Botanical Garden based on leaf morphological characters Mahfut Mahfut; Tundjung Tripeni Handayani; Sri Wahyuningsih; Sukimin Sukimin
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v5i1.2938

Abstract

Lampung Indonesia is one of the provinces on the island of Sumatra, which has a flora conservation area located in Liwa Botanical Garden, West Lampung Regency. One of the flora species in the area with a high level of diversity is the orchid plant. The Dendrobium is the largest orchid species, where the public widely hunts this orchid for an economic reason due to the beauty of its various flower shapes and colors. Given the high level of human encroachment, this can threaten the existence of natural orchid plants, causing loss of their original habitat and causing the extinction of orchid species. Based on data in Liwa Botanical Garden, there has been no further identification of natural orchids as a plant conservation effort. To protect and immediately save these natural orchids, it is necessary to identify the Dendrobium orchid species based on the leaf morphological approach. This research will be conducted in April-September 2020. Observations of leaf morphology include leaf shape, leaf tip shape, leaf base shape, leaf edge, leaf reinforcement, leaf arrangement, leaf surface texture, leaf symmetry, and leaf sitting based on guidelines for ornamental plant characterization books. Observations as secondary data were added to the character of the pseudobulb shape and orchid habitat. Observations as secondary data were added on the habitat character of pseudobulbs and orchids. The implementation of this activity is still in the period of the Covid-19 pandemic, so most of the activities are carried out online. The coaching and training phase was conducted through internal virtual discussions on Tuesday, July 7, 2020, while the coaching phase was conducted through meetings or verbal communication via telephone groups and WhatsApp. Based on the evaluation data, it is known that there is an increase in knowledge and understanding of training participants by 21.64 points. The percentage increase in value before and after training is 34.72%. Orchid-related activities at Liwa Botanical Garden were never carried out, thus imparting knowledge to Liwa Botanical Garden technicians and workers on the development of natural orchid identification methods based on the morphological character in local natural resource conservation in the 4.0 industrial revolution era.
KETAHANAN KULTIVAR BUAH TOMAT (Solanum lycopersicum L.) TERHADAP JAMUR Colletotrichum acutatum J. H. Simmonds PENYEBAB PENYAKIT ANTRAKNOSA Aryani Refiliya; Yulianty Yulianty; Martha Lulus Lande; Sri Wahyuningsih
Jurnal Medika Malahayati Vol 4, No 3 (2020): Volume 4 Nomor 3
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.212 KB) | DOI: 10.33024/jmm.v4i3.2918

Abstract

Tomat merupakan tanaman semusim seperti halnya tanaman cabe, terong dan sebagainya. Salah satu penyebab menurunnya produksibuah tomat adalah penyakit antraknosa yang disebabkan oleh jamur Colletotrichumacutatum. Penelitian ini dilaksanakan untuk mengetahui ketahanan buah tomat terhadap serangan penyakit antraknosa oleh jamur Colletotrichumacutatum. Penelitian ini dilaksanakan di Laboratorium Mikrobiologi dan Laboratorium Botani, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung pada bulan Januari sampai dengan Februari 2020. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan buah tomat yaitu rampai, tomat bandung, tomat ceri, dan tomat biasa dengan menggunakan 6 ulangan. Parameter yang diukur yaitu diameter bercak jamur Colletotrichumacutatum, keterjadian penyakit dan keparahan penyakit antraknosa pada masing-masing buah tomat, serta jumlah konidia jamur Colletotrichumacutatum. Hasil penelitian menunjukkan bahwa keterjadian dan keparahan penyakit yang lebih kecil terdapat padatomatbandung sedangkan jumlah konidia yang lebih sedikit terdapat pada rampai.
Co-Authors Achmad Arifiyanto Admi Syarif Agustrina, Rochmah Aryani Refiliya Bambang Irawan Deby Arifiani Emantis Rosa Endang Nurcahyani Eti Ernawiati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fania Nur Izzati Fania Nur Izzati Fersiana Riska Devilia Garri Abdi Nusantara Hardoko Insan Qudus Hasti Maulidya Fassya Herta M. Manullang Istiqomah istiqomah Kinasih Cahyono Kurnia Muludi Mahfut Martha Lulus Lande Martha Lulus Lande Martha Lulus Lande Mitha Valentina Treesya Panjaitan Muhammad Irfan Ardiansyah Putri Kendari Ratri Mauluti Larasati Sahira Josy Arifannisa Sukimin Sukimin Sukimin Sukimin Sumardi, Sumardi Sutyarso Tundjung T. Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Valentina Dwi Anggita Sari Wanda Amelia Widi Aryani Yoga Aji Saputra Yulianty Yulianty - Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zulkifli Zulkifli Zulkifli Zulkifli