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Haryanto Dhanutirto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Penapisan Aktivitas dan Senyawa Antidiabetes Ekstrak Air Daun Dandang Gendis (Clinacanthus nutans) Nurulita, Yuana; Dhanutirto, Haryanto; Soemardji, Andreanus A.
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.499 KB) | DOI: 10.31258/jnat.10.2.98-103

Abstract

An investigation of the antidiabetic activity of Aqueous Extract and its fractions from a different polarity solvent:ethanol, ethyl acetate, and n-hexane of Dandang Gendis Leaves (Clinacanthus nutans Lindau) on Mile SwissWebster Mice has been carried out. The effect of aqueous extract of dose of 50, 100, and 150 mg/kg BW wastested on glucose serum level of alloxan induced that mice. Its fractions were tested by glucose tolerance method.Aqueous extract (150 mg/kg BW) significantly decreased blood glucose serum level (pd”0,05) that had the sameeffect as oral glibenclamide (0,65 mg/kg BW) administrated once a day for 9 days. Aqueous extract that wasfractionated has 5 fraction. Fraction of ethanol extract that was insoluble in ethyl acetate at a dose of 100 mg/kgBW was the most active fraction, that significantly (p<0,05) decreased blood glucose serum level of mice atglucose tolerance method of 18,4% in 3 hours after 2 g/kg BW of glucose administration. Thin layer chromatography(TLC) of that fraction ethanol 95%-chloroform 9:2 as eluent gave 5 spots under ultraviolet ray and showed spesificcompounds by qualitative test. Spot 1 (steroid) gave Rf 0,14; spot 2 and spot 3 that gave Rf 0,53 and 0, 67 wereflavonoid, spot 4 (triterpenoid) gave Rf 0,77; and spot 5 (chlorophyll) gave Rf 0,89. This research show that this plantcan used for hipoglicemic traditional medicine.
Mutation and Characterization of an Albino Mutant of Monascus sp. Isolated from the Cikapundung River, Bandung TIANA MILANDA; MARLIA SINGGIH WIBOWO; TUTUS GUSDINAR; HARYANTO DHANUTIRTO
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.286 KB) | DOI: 10.5454/mi.1.1.5

Abstract

Monascus sp. isolated from Cikapundung River, Bandung was mutated using ethyl methanesulfonate (2.5%, 90 min). Previously, this wild type was identified as Monascus purpureus ITBCC-HD-F001 employing random amplification polymorphic DNA (RAPD). Stability of the mutant was observed using color consistency and mutant stability (sub-culturing for five generations) tests. Genetic variation of the mutant (M. purpureus ITBCC-HDF002) was confirmed by RAPD. One of the DNA bands of 1150 bp was found in the albino mutant but not in the wild type, so it was considered as a genetic variation resulting from the mutation process. The albino mutant was characterized by comparing the growth curve, biomass production curve, and the monascidin A production curve of both strains i.e. wild type and the albino mutant. Monascidin A production of the mutant was higher than that of the wild type.
Co-Authors Andreanus A. Soemardji Marlia Singgih Wibowo TIANA MILANDA TUTUS GUSDINAR Yuana Nurulita