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Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation Hardiyanti, Citra; Khairullinas, Khairullinas; Lumban, Jeky Sasemar; Nugroho, Titania Tjandrawati; Nurulita, Yuana
Jurnal Kimia Sains dan Aplikasi Vol 23, No 3 (2020): Volume 23 Issue 3 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2803.553 KB) | DOI: 10.14710/jksa.23.3.89-95

Abstract

An antibiotic-resistant and multidrug-resistant (MDR) issue open the role of researchers to continue to search for natural potential as a source of new antimicrobials. One of the potential fungi isolates that can produce antimicrobial active compounds from Indonesian tropical peat soils is Penicillium sp. LBKURCC34. In this study, the production of antimicrobial compounds from local isolates was carried out by batch fermentation method in liquid media with the addition of biotic elicitors to increase the extraction activity and yield. This study aims to optimize the results based on the time the elicitor is added. Staphylococcus aureus was used as a biotic elicitor, which was added on days 2, 3, and 4 in the production of antibiotics by fermentation incubation of 6-14 days. The antibiotic production media was extracted with ethyl acetate and evaporated. The antimicrobial test was carried out by the disk diffusion method against pathogenic bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Staphylococcus epidermidis using three crude extract contents (19; 38; and 57 µg/disc). Amoxicillin® was used as a positive control (10 µL/disc). The results showed that the addition of S. aureus biotic elicitor extended the log phase growth of the fungus Penicillium sp. LBKURCC34. The optimum condition of production was obtained by adding initiator treatment on the 3rd day for 14 days incubation with the highest yield and could inhibit the growth of all pathogenic microbes.
Sintesis dan Uji Antibakteri Senyawa Bromo Kalkon Piridin Jasril, Jasril; Teruna, Hilwan Yuda; Zamri, Adel; Alfatos, Darian; Yuslinda, Elka; Nurulita, Yuana
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.23 KB) | DOI: 10.31258/jnat.14.3.172-175

Abstract

Three pyridine chalcones including (Z)-1-(4-bromophenyl)-3-(pyridin-2-yl)prop-2-en-1-one (1), (Z)-1-(4-bromophenyl)-3- (pyridin- 3-yl)prop-2-en-1-one (2) and (Z)-1-(4-bromophenyl)-3-(pyridin-4-yl)prop-2-en-1-one (3) were synthesized by aldol condensation reactions from pyridinecarbaldehyde with 4-bromoacetophenone. In antibacterial assay, compound 3 exhibited strong activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli with the inhibition zone of 19.9; 19.5, and 17.5 mm, respectively.
Penapisan Aktivitas dan Senyawa Antidiabetes Ekstrak Air Daun Dandang Gendis (Clinacanthus nutans) Nurulita, Yuana; Dhanutirto, Haryanto; Soemardji, Andreanus A.
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.499 KB) | DOI: 10.31258/jnat.10.2.98-103

Abstract

An investigation of the antidiabetic activity of Aqueous Extract and its fractions from a different polarity solvent:ethanol, ethyl acetate, and n-hexane of Dandang Gendis Leaves (Clinacanthus nutans Lindau) on Mile SwissWebster Mice has been carried out. The effect of aqueous extract of dose of 50, 100, and 150 mg/kg BW wastested on glucose serum level of alloxan induced that mice. Its fractions were tested by glucose tolerance method.Aqueous extract (150 mg/kg BW) significantly decreased blood glucose serum level (pd”0,05) that had the sameeffect as oral glibenclamide (0,65 mg/kg BW) administrated once a day for 9 days. Aqueous extract that wasfractionated has 5 fraction. Fraction of ethanol extract that was insoluble in ethyl acetate at a dose of 100 mg/kgBW was the most active fraction, that significantly (p<0,05) decreased blood glucose serum level of mice atglucose tolerance method of 18,4% in 3 hours after 2 g/kg BW of glucose administration. Thin layer chromatography(TLC) of that fraction ethanol 95%-chloroform 9:2 as eluent gave 5 spots under ultraviolet ray and showed spesificcompounds by qualitative test. Spot 1 (steroid) gave Rf 0,14; spot 2 and spot 3 that gave Rf 0,53 and 0, 67 wereflavonoid, spot 4 (triterpenoid) gave Rf 0,77; and spot 5 (chlorophyll) gave Rf 0,89. This research show that this plantcan used for hipoglicemic traditional medicine.
Potensi Metabolit Sekunder dari Trichoderma sp. LBKURCC22 Tanah Gambut Hutan Sekunder Sebagai Antibiotik Narasswati, Nungki; Oktavia, Rani; Nenci, Nuryani; Eryanti, Yum; Nugroho, Titania Tjandrawati; Nurulita, Yuana
Chimica et Natura Acta Vol 5, No 2 (2017)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.778 KB) | DOI: 10.24198/cna.v5.n2.14692

Abstract

Trichoderma sp. LBKURCC22 adalah isolat lokal yang diisolasi dari lahan hutan rawa gambut sekunder. Isolat ini berpotensi menghasilkan metabolit sekunder. Tujuan dari penelitian ini adalah untuk menggali potensi metabolit sekunder dari isolat Trichoderma sp. LBKURCC22 dalam fermentasi batch. Metabolit sekunder diekstraksi dengan etil asetat. Ekstrak diuapkan, kemudian diperoleh ekstrak kasar. Analisis dilakukan dengan uji fitokimia, KLT, dan KCKT. Selanjutnya, aktivitas antibiotik dilakukan terhadap Escherichia coli dan Staphylococcus aureus dengan metode dilusi. Uji fitokimia menunjukkan bahwa ekstrak mengandung alkaloid. Namun, aktivitas antibakteri, senyawa metabolit sekunder dari ekstrak tidak aktif terhadap bakteri-bakteri yang diuji. Hasil penelitian menunjukkan bahwa KLT dengan eluen etil asetat:n-heksana (6:4) setelah disemprot dengan 0,5% p-anisaldehid menunjukkan tidak adanya peptaibol pada noda P1 (Rf :0,78), P2 (Rf 0,65), dan P3 (Rf 0). Analisis KCKT menghasilkan ekstrak mengandung satu puncak pada waktu retensi (tR) 4,115 menit pada 214 nm dan 4,106 menit pada 227 nm. Penelitian ini menunjukkan bahwa isolat Trichoderma sp. LBKURCC22 tidak memproduksi metabolit sekunder yang berpotensi sebagai antibiotik. Perlu dilakukan analisis lebih lanjut untuk mengeksplorasi potensi isolat ini.
Penerapan teknologi biopori dalam pencegahan banjir dan kekeringan yang sekaligus pembuatan biokompos di Kelurahan Delima Kecamatan Tampan Pekanbaru Andi Dahliaty; Yuana Nurulita; Titania Tjandrawati Nugroho; Sri Helianty
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.255-261

Abstract

Flooding is a problem that almost every year affects urban and rural areas. Generally, in urban areas, floods are caused more by the lack of smooth flow of water (sewage) due to waste dumped into the water stream and the reduction of water catchment areas. The Delima Subdistrict of Tampan Subdistrict has a simple housing area that has a limited home yard measuring around 7m x13m up to 10m x 15m per house unit. To meet clean water needs, this area uses bore wells which over time will reduce underground water reserves. The housing area is sometimes disemenised, which results in a lack of infiltration of rain water so that it is prone to flooding and drought. To overcome this, the community can apply biopore infiltration technology. This service activity aims to introduce biopore infiltration technology and invites the community to apply it and make biocompost from the infiltration hole. The method of service activities was carried out with material delivery techniques and discussions as well as demonstrations or practices in making biopori infiltration holes as an effort to overcome floods and droughts in settlements around the Delima Village, Tampan District, Pekanbaru. From the results of this activity it is known that the target community seemed enthusiastic about the material presented and there was an increase in the knowledge and insights of the participants about biopori absorption technology by around 80%.
Teknologi Biokompos dalam Biopori vertikal di Kelurahan Pematang Pudu Kecamatan Mandau Kabupaten Bengkalis Andi Dahliaty; Yuana Nurulita; Titania Tjandrawati Nugroho
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.156-162

Abstract

Pematang Pudu Village is one of the villages in Mandau District, Bengkalis Regency which is approximately 116 km from Pekanbaru city. Based on information from residents of RT 02 RW 10 Km 5 Jalan Rangau, Pematang Pudu Village often floods. Floods in this area can be caused by overflowing river water, reduced rainwater catchment areas due to construction and land clearing. This biopore infiltration hole is a simple technology that does not require expensive and effective rainwater absorption. In addition, through this technology, it can reduce organic waste and make biocompost to be used as organic fertilizer that can fertilize plants. The purpose of this service activity is to provide knowledge and skills to the community about the benefits of vertical biopore technology and increase public awareness to prevent flooding. The method used in this service activity is the delivery of material using powerpoint media and field practice and involving community members in RT 02 RW 10 Pematang Pudu Village. The results of this service activity show that 38% of socialization participants have a score range between 75-80, 13.66% of socialization participants have a range of values between 85-90, and 28% of socialization participants have a range of values between 95-100. It can be stated that the provision of training has an effect on increasing public knowledge about biopore infiltration holes.