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All Journal Indonesian Journal of Biotechnology BAKTIMU : Jurnal Pengabdian Kepada Masyarakat
Dinar Mindrati Fardhani
Universitas ‘Aisyiyah Yogyakarta
Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Legume Nodulating Bacterium, Achromobacter xylosoxidans Found in Tropical Shrub Agroecosystem, Sumatera, Indonesia Wedhastri, Sri; Fardhani, Dinar Mindrati; Kabirun, Siti; Widada, Jaka; Widianto, Donny; Evizal, Rusdi; Prijambada, Irfan Dwidya
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.716 KB)

Abstract

Legume nodulating bacteria (LNB), known also as rhizobia, are soil bacteria, which are able to form rootnodules and fi x nitrogen in the leguminous plants. The LNB availability in the soil depends on the type ofagroecosystem, where plant grows. In this study, we isolated LNB from the shrub agroecosystem in Sumatera,Indonesia, and obtained four selected bacterial strains. Among them, the isolate UGM48a formed root nodulein Macroptilium atropurpureum and showed highest number of nitrogenase activity. UGM48a also contains nifHand nodA genes. An analysis of the PCR-amplifi ed 16S rDNA and BLASTn analysis showed that UGM48adisplayed 96% similarity with Achromobacter xylosoxidans. In addition, UGM48a were successfully nodulatedGlycine max (L.) merr var. wilis. This is the fi rst report detecting A. xylosoxidans as nodule-forming species forGlycine max possesing the positive copy of nodA gene.Keywords : Legume Nodulating Bacteria, shrub agroecosystem, Achromobacter xylosoxidans, nodA, Glycine max
Legume Nodulating Bacterium, Achromobacter xylosoxidans Found in Tropical Shrub Agroecosystem, Sumatera, Indonesia Sri Wedhastri; Dinar Mindrati Fardhani; Siti Kabirun; Jaka Widada; Donny Widianto; Rusdi Evizal; Irfan Dwidya Prijambada
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.716 KB) | DOI: 10.22146/ijbiotech.7879

Abstract

Legume nodulating bacteria (LNB), known also as rhizobia, are soil bacteria, which are able to form rootnodules and fi x nitrogen in the leguminous plants. The LNB availability in the soil depends on the type ofagroecosystem, where plant grows. In this study, we isolated LNB from the shrub agroecosystem in Sumatera,Indonesia, and obtained four selected bacterial strains. Among them, the isolate UGM48a formed root nodulein Macroptilium atropurpureum and showed highest number of nitrogenase activity. UGM48a also contains nifHand nodA genes. An analysis of the PCR-amplifi ed 16S rDNA and BLASTn analysis showed that UGM48adisplayed 96% similarity with Achromobacter xylosoxidans. In addition, UGM48a were successfully nodulatedGlycine max (L.) merr var. wilis. This is the fi rst report detecting A. xylosoxidans as nodule-forming species forGlycine max possesing the positive copy of nodA gene. Keywords : Legume Nodulating Bacteria, shrub agroecosystem, Achromobacter xylosoxidans, nodA, Glycine max
PEMENUHAN GIZI KELUARGA DI MASA PANDEMI COVID-19 MELALUI BUDIDAYA IKAN DAN TANAMAN SAYUR DALAM EMBER : FULFILLMENT OF FAMILY NUTRITION DURING THE COVID-19 PANDEMIC THROUGH FISH AND VEGETABLE CULTIVATION IN BUCKETS Ika Afifah Nugraheni; Dinar Mindrati Fardhani
BAKTIMU : Jurnal Pengabdian Kepada Masyarakat Vol 2 No 1 (2022)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/bm.v2i1.376

Abstract

Dampak pandemi COVID-19 telah mempengaruhi semua sektor kehidupan. Sektor ekonomi dan ketahanan pangan merupakan beberapa sektor yang terdampak COVID-19. Pendapatan dan daya beli masyarakat mengalami penurunan yang berimbas ke sektor ketahanan pangan. Masyarakat harus memenuhi gizi keluarga di tengah keterbatasan pendapatan. Dengan demikian, alternatif sumber pangan yang murah dan terjangkau diperlukan oleh masyarakat, termasuk juga oleh anggota Balai Sakinah ‘Aisyiyah (BSA) Desa Treko, dalam pemenuhan gizi keluarga. Budidaya ikan dan tanaman sayur dalam ember (budikdamber) dapat menjadi sebuah solusi dalam rangka pemenuhan sumber pangan dan gizi di masa pandemi. Tujuan dari kegiatan pengabdian ini adalah memberikan edukasi mengenai sistem pertanian budikdamber kepada BSA Desa Treko. Peserta dalam kegiatan pengabdian ini sebanyak 11 orang yang terdiri dari ibu rumah tangga. Pelaksanaan pengabdian dilakukan secara luring dengan memberikan edukasi dan pelatihan budikdamber. Tahapan pelaksanaan kegiatan, meliputi tahap persiapan media aquaponik tanaman sayur, tahap persiapan budidaya ikan budikdamber, tahap pemeliharaan, dan tahap pemanenan. Tanaman sayur dipilih tanaman kangkung, sedangkan ikan yang dibudidayakan berupa ikan lele. Kegiatan pengabdian terlaksana dengan baik, dan mitra memberikan respon positif selama pelaksanaan. Anggota BSA sangat antusias mencoba budikdamber di rumah masing-masing. Sebanyak 72,73% anggota BSA masih melakukan pemeliharaan hingga masa panen tanaman kangkung dan ikan lele. Tanaman sayur dan ikan lele hasil budikdamber diharapkan mampu menjadi sumber pangan dan gizi tambahan keluarga dalam rangka menjaga kesehatan tubuh dan menjaga daya tahan tubuh selama menghadapi COVID-19.
Co-Authors Donny Widianto Donny Widianto Ika Afifah Nugraheni Irfan Dwidya Prijambada JAKA WIDADA Rusdi Evizal Siti Kabirun SITI KABIRUN Sri Wedhastri Sri Wedhastri