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Journal : Serambi Biologi

The Activity Xylanase Enzyme Thermophilic Bacteria SSA 2 in Starter Variation Elfah rahma; Irdawati Irdawati
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11853RF00

Abstract

Xylanase is extracellular enzyme that hydrolyzes xylan into xylose and xylooligosaccharides. The bacteria that produce the xylanase enzyme, namely SSA 2 isolate from the Sapan River Aro Solok Selatan hot spring, has a temperature of 75ºC and a pH of 8 or alkaline. Thermophilic bacteria can produce xylanase enzymes under optimum environmental conditions. Environmental optimization can be done by optimizing media composition, dissolved oxygen content, aeration, agitation, starter concentration, pH and temperature. This study aims to determine the specific activity of the xylanse enzyme at the starter concentration. The starter concentrations used were 2%, 4%, 6%, 8% and 10%. The highest specific activity of the xylanse enzyme was at a starter concentration of 4% (0.916 U/mL) this indicates that a low starter concentration can lead to good microbial growth.
THE EFFECT OF TEMPERATURE ON THE ACTIVITIES OF AMOBIL XILANASE ENZYMES IN THE PAPER BIOBLEACHING PROCESS (PULP) Irdawati Irdawati; Fauzana Ahmad; Syamsuardi Syamsuardi; Agustien Agustien; Y Rilda
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/6023RF00

Abstract

Abstrak. Enzim xilanase merupakan enzim extraseluler yang mampu menghidrolisis xilan menjadi xilosa. Isolat SSA 2 merupakan isolat dari bakteri termofilik hasil isolasi dari air panas sapan sungai aro, yang memiliki kemampuan menghasilkan enzim xilanase. penggunaan xilanase merupakan salah satu alternative mengurangi pemakain clorin dalam proses pemutihan pulp. xilanase diamobilisasi dengan menggunkan NA alginate. pulp difermentasi dengan suhu 50ºC, 55ºC, 60ºC, 65ºC, 70 ºC, 75 ºC, 80ºC, selama 6 jam, suhu optimum yang menghasilkan bilangan kappa terendah dan aktivitas enzim yang tertinggi pada suhu 60ºC.