Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Lutfi Sukma Pratiwi
Universitas Ahmad Dahlan
Health Professions

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Uji Kualitas DNA Darah Pada Kertas Whatman Yang Diisolasi Dengan CHELEX-100 Serta Variasi Waktu Penyimpanan Rita Maliza; Lutfi Sukma Pratiwi; Dyah Aryani Perwitasari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7936

Abstract

DNA extraction from dried blood spots was used for molecular analysis. Dried blood spots sample usually used FTA cards, but short-term use would come at a cost. This study aims to identify DNA extraction quality from dried blood spots in Whatman filter paper as an alternative storage sample. This study used 15 samples with three different storage time for 1, 3, and 7 days on 4°C. Dried blood samples were extracted using the Chelex-100 method, and qualitative were identified by electrophoresis. The DNA extraction was used as a template on PCR amplification of the gapdh gene. The result showed that DNA extraction showed band from 1, 3, and 7 days, and PCR amplification showed bands in 200 bp. In conclusions, DNA from dried blood samples was stored for 1, 3 and 7 days in Whatman filter paper were successfully extracted by the Chelex-100 method and can be used as a DNA template for PCR amplification.
Co-Authors Dyah Aryani Perwitasari Maliza, Rita