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Journal : Borneo Journal of Medical Laboratory Technology

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Efektivitas Antimikroba Ekstrak Biji Manjakani (Quercus Infectoria) Terhadap Penghambatan Candida sp.: Antimicrobial Effectiveness of Manjakani Seed Extract (Quercus infectoria) Against Inhibition of Candida sp. Djailani, Armivia Puteri; Aina, Ganea Qorry; Harlita, Tiara Dini
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.5848

Abstract

Biji manjakani secara empiris digunakan untuk mengobati berbagai macam penyakit sebagai antiinflamasi, antibakteri dan antijamur. Tujuan penelitian ini Menganalisis efektivitas antimikroba ekstrak biji manjakani (Q. infectoria) terhadap penghambatan Candida sp. Penelitian ini merupakan adalah penelitian eksperimental dengan acak lengkap (RAL) dua faktor. Faktor 1 yaitu jenis pelarut (n­-heksan, etil asetat dan etanol 96%) dan faktor 2 yaitu konsentrasi (50%, 75% dan 100%). Sampel yang digunakan adalah ekstrak biji manjakani yang diperoleh dengan maserasi bertingkat menggunakan pelarut n-heksan, etil asetat dan etanol 96%. Pada penelitian ini mikroba uji yang digunakan adalah Candida sp. uji antimikroba menggunakan metode difusi agar (Kirby Bauer), dengan kontrol positif flukonazol 150 mg dan kontrol negatif aquadest.Analisis data yang digunakan adalah One Way Anova dan uji lanjutan metode Duncan dengan taraf signifikasi 0,05. Hasil yang didapatkan pada penelitian ini terdapat pengaruh diameter zona hambat ekstrak biji manjakani terhadap penghambatan Candida sp. Konsentrasi yang paling efektif pada etanol 96% konsentrasi 100% sebesar 14,80 mm dan efektivitas sebesar 47,31% dengan kontrol positif flukonazol 150 mg sebesar 31,28 mm. Dapat disimpulkan bahwa ekstrak biji manjakani (Q. infectoria) memiliki efektivitas antimikroba terhadap penghambatan Candida sp., namun potensinya masih dibawah flukonazol.
Aktivitas Antibakteri Kombinasi Ekstrak Biji Manjakani (Quercus infectoria) Dan Daun Sirih Merah (Piper crocatum) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae: Antibacterial Activity Combination of Manjakani Seed Extract (Quercus infectoria) and Red Betel Leaf (Piper crocatum) Against the Growth of Klebsiella pneumoniae. Ashari, Ayu; Harlita, Tiara Dini; Aina, Ganea Qorry
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6075

Abstract

Potential plants found in Indonesia that can be used for the treatment of urinary tract infections include: Manjakani seeds (Quercus infectoria) and red betel leaves (Piper crocatum). Manjakani seeds are pharmacologically claimed to have biological activities such as anti-inflammatory, antibacterial and antifungal. Red betel leaf has the potential as an antibacterial in the treatment of infectious diseases. The purpose of this study was to determine the antibacterial activity of the combination of manjakani seed extract and red betel leaf in inhibiting the growth of Klebsiella pneumoniae bacteria. The type of research used istrue experiment study with a completely randomized design (CRD) with three factors, namely the type of solvent n-hexane, ethyl acetate, ethanol 96%, ratio (1:1,1:2,2:1) and concentration (75%,100%) with each treatment being repeated twice. This research was conducted in the microbiology laboratory, Labkes, East Kalimantan. The population in this study is the manjakani plant and red betel plant and tested using the agar diffusion technique method Kirby Bauer with positive control (Ciprofloxacin) and negative control (aquadest). Data analysis used is One Way ANOVA and advanced test methods Duncan with a significance level of 0,05. The results obtained showed that there was an effect of antibacterial activity of Manjakani seed extract and red betel leaf on growth K. pneumoniae. The most effective concentration was found in ethanol 96% extract, (2:1 combination of 100% concentration) of 13,97 mm and an effectiveness of 55,74% with a positive control ciprofloxacin 5µg of 25,06 mm. It can be concluded that the combined extract of manjakani seeds and red betel leaves has antibacterial activity against bacteria Klebsiella pneumoniae,but the potential is still low of ciprofloxacin.
Co-Authors Aina, Ganea Qorry Ardiana Finda Widya Rahmawati Ashari, Ayu Azahra, Sresta Djailani, Armivia Puteri Ganea Qorry Aina I Gede Andika Sukarya Khairani, Ulfiyah Mitha Anggreni Nurul Anggrieni Rahmawati, Yosiana Sari, Renita Tasya Choirifah Parwanti Yusran, Dini Indriaty